P43570 (GYP8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase-activating protein GYP8

Gene names

Name:	GYP8
Ordered Locus Names:	YFL027C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous	Present with 573 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 Rab-GAP TBC domain.

Ontologies

Keywords
Molecular function	GTPase activation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	vesicle-mediated transport Inferred from genetic interaction. Source: SGD
Cellular component	intracellular Inferred from direct assay. Source: SGD
Molecular function	Rab GTPase activator activity Inferred from physical interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	GTPase-activating protein GYP8		PRO_0000208018
Regions
	Domain	69 – 281	213	Rab-GAP TBC

Sequences

	Sequence		Length	Mass (Da)
	P43570 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C93C57821B50F1E5	FASTA	497	57,619
	10 20 30 40 50 60 MPLRSLFHTN QSSHDKDALT RGGYNAYLES LSRCDSGKAE EQKGKVISKL LEKKDVRALR 70 80 90 100 110 120 YIGLGPLGFV NNSLRKDCWY ELLASQLLID DATEYITPVE KHKDEGQVIL DAERSFGGIV 130 140 150 160 170 180 DKNLKLQLRK LLVELITRVL RKYPTLNYYQ GYHDIVSVFI MCFSWNVTKE NGLELENLSL 190 200 210 220 230 240 QEEIDMERLF YCIEAFTLLY LRDFMMNSLD FSFEQLRVIS SLIKESNMKF YNLFKFDENE 250 260 270 280 290 300 PLFAIGSILT IFAHNLKPID SGDNNLHKIL FQIFDMTISM QSMRLPLIIY KNLLLQNASE 310 320 330 340 350 360 ISKQIEANSD FFENDFDLRH GAIQTVLQKK LYDEALWEEV LQITRKDATT ASKKALKRVS 370 380 390 400 410 420 LNKYSALLNT ACGKPGCFDM STIIFYLSEQ TKMNEHYKEE KYHGVAARSK TRALVQRLGH 430 440 450 460 470 480 FLPSKYNRWS KISLLIGIVA ILYQLRTTRS LSLVLNLRYM ISTKLKDLSH ININLHHVSH 490 IWVDPIRDIL KLGHPTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09211.1. AY693137 Genomic DNA. Translation: AAT93156.1. BK006940 Genomic DNA. Translation: DAA12413.1.
PIR	S56227.
RefSeq	NP_444296.1. NM_001179939.1.
3D structure databases
ProteinModelPortal	P43570.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5431N.
IntAct	P43570. 2 interactions.
MINT	MINT-485987.
STRING	P43570.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL027C; YFL027C; YFL027C.
GeneID	850517.
KEGG	sce:YFL027C.
NMPDR	fig|4932.3.peg.2253.
Organism-specific databases
CYGD	YFL027c.
SGD	S000001867. GYP8.
Phylogenomic databases
eggNOG	fuNOG06258.
GeneTree	EFGT00050000006740.
OMA	HININLH.
OrthoDB	EOG49KK0G.
Gene expression databases
ArrayExpress	P43570.
Genevestigator	P43570.
GermOnline	YFL027C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000195. RabGAP/TBC_dom. [Graphical view]
Pfam	PF00566. TBC. 1 hit. [Graphical view]
SUPFAM	SSF47923. RabGAP_TBC. 1 hit.
PROSITE	PS50086. TBC_RABGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966242.

Entry information

Entry name

GYP8_YEAST

Accession

Primary (citable) accession number: P43570
Secondary accession number(s): D6VTK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program