P43569 (CAF16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCR4-associated factor 16

Gene names

Name:	CAF16
Ordered Locus Names:	YFL028C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with CCR4 and SSN2. Ref.3
Subcellular location	Cytoplasm. Nucleus Ref.4.
Miscellaneous	Present with 1620 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the ABC transporter superfamily. Contains 1 ABC transporter domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.3. Source: SGD
Cellular component	CCR4-NOT complex Inferred from direct assay Ref.3. Source: SGD cytoplasm Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SAH1	P39954	3	EBI-22808,EBI-16451

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	CCR4-associated factor 16		PRO_0000093464
Regions
	Domain	7 – 249	243	ABC transporter
	Nucleotide binding	41 – 48	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P43569 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2250CC15F9CE1354	FASTA	289	33,090
	10 20 30 40 50 60 MVSQFAIEVR NLTYKFKESS DPSVVDINLQ IPWNTRSLVV GANGAGKSTL LKLLSGKHLC 70 80 90 100 110 120 LDGKILVNGL DPFSPLSMNQ VDDDESVEDS TNYQTTTYLG TEWCHMSIIN RDIGVLELLK 130 140 150 160 170 180 SIGFDHFRER GERLVRILDI DVRWRMHRLS DGQKRRVQLA MGLLKPWRVL LLDEVTVDLD 190 200 210 220 230 240 VIARARLLEF LKWETETRRC SVVYATHIFD GLAKWPNQVY HMKSGKIVDN LDYQKDVEFS 250 260 270 280 EVVNAKVNGQ VAFENDNNKV VISKVNSLHP LALEWLKRDN QIPDKEIGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Characterization of CAF4 and CAF16 reveals a functional connection between the CCR4-NOT complex and a subset of SRB proteins of the RNA polymerase II holoenzyme." Liu H.-Y., Chiang Y.-C., Pan J., Chen J., Salvadore C., Audino D.C., Badarinarayana V., Palaniswamy V., Anderson B., Denis C.L. J. Biol. Chem. 276:7541-7548(2001) [PubMed: 11113136] [Abstract] Cited for: INTERACTION WITH CCR4 AND SSN2.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09210.1. BK006940 Genomic DNA. Translation: DAA12412.1.
PIR	S56226.
RefSeq	NP_116625.1. NM_001179938.1.
3D structure databases
ProteinModelPortal	P43569.
SMR	P43569. Positions 6-248.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5946N.
IntAct	P43569. 3 interactions.
STRING	P43569.
Proteomic databases
PeptideAtlas	P43569.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL028C; YFL028C; YFL028C.
GeneID	850516.
KEGG	sce:YFL028C.
NMPDR	fig|4932.3.peg.2252.
Organism-specific databases
CYGD	YFL028c.
SGD	S000001866. CAF16.
Phylogenomic databases
eggNOG	fuNOG07136.
GeneTree	EFGT00050000001692.
HOGENOM	HBG316888.
OMA	LETWASH.
OrthoDB	EOG42NN8P.
Gene expression databases
ArrayExpress	P43569.
Genevestigator	P43569.
GermOnline	YFL028C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K12608.
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS00211. ABC_TRANSPORTER_1. False negative. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966239.

Entry information

Entry name

CAF16_YEAST

Accession

Primary (citable) accession number: P43569
Secondary accession number(s): D6VTK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program