P43568 (CAK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase CAK1
EC=2.7.11.22

Alternative name(s):
CDK-activating kinase

Gene names

Name:	CAK1
Synonyms:	CIV1
Ordered Locus Names:	YFL029C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1 phase of mitotic cell cycle Inferred from mutant phenotype Ref.1PubMed 8752211. Source: SGD G2/M transition of mitotic cell cycle Inferred from mutant phenotype Ref.1PubMed 8752211. Source: SGD meiosis Inferred from genetic interaction PubMed 11739722. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 9819350. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein kinase activating kinase activity Inferred from direct assay PubMed 10373527Ref.1PubMed 8752211. Source: SGD cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC28	P00546	3	EBI-3953,EBI-4253

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Serine/threonine-protein kinase CAK1		PRO_0000085690
Regions
	Domain	1 – 368	368	Protein kinase
Sites
	Active site	156	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	205	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P43568 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 852AFD1E95B90C42	FASTA	368	42,186
	10 20 30 40 50 60 MKLDSIDITH CQLVKSTRTA RIYRSDTYAI KCLALDFDIP PHNAKFEVSI LNKLGNKCKH 70 80 90 100 110 120 ILPLLESKAT DNNDLLLLFP FEEMNLYEFM QMHYKRDRRK KNPYYDLLNP SIPIVADPPV 130 140 150 160 170 180 QKYTNQLDVN RYSLSFFRQM VEGIAFLHEN KIIHRDIKPQ NIMLTNNTST VSPKLYIIDF 190 200 210 220 230 240 GISYDMANNS QTSAEPMDSK VTDISTGIYK APEVLFGVKC YDGGVDVWSL LIIISQWFQR 250 260 270 280 290 300 ETSRMGHVPA MIDDGSDDMN SDGSDFRLIC SIFEKLGIPS IQKWEEVAQH GSVDAFVGMF 310 320 330 340 350 360 GADGDGKYVL DQEKDVQISI VERNMPRLDE IADVKVKQKF INCILGMVSF SPNERWSCQR ILQELEKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Cdk-activating kinase (CAK) from budding yeast." Kaldis P., Sutton A., Solomon M.J. Cell 86:553-564(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-205, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60192 Genomic DNA. Translation: AAB17052.1. D50617 Genomic DNA. Translation: BAA09209.1. BK006940 Genomic DNA. Translation: DAA12411.1.
PIR	S56225.
RefSeq	NP_116624.1. NM_001179937.1.
3D structure databases
ProteinModelPortal	P43568.
SMR	P43568. Positions 25-363.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1637N.
IntAct	P43568. 5 interactions.
MINT	MINT-400933.
STRING	4932.YFL029C.
Proteomic databases
PaxDb	P43568.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL029C; YFL029C; YFL029C.
GeneID	850515.
KEGG	sce:YFL029C.
Organism-specific databases
CYGD	YFL029c.
SGD	S000001865. CAK1.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000111296.
KO	K06667.
OMA	SIDITHC.
OrthoDB	EOG4V46HW.
Enzyme and pathway databases
BRENDA	2.7.11.22. 984.
Gene expression databases
Genevestigator	P43568.
GermOnline	YFL029C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P43568.
ChEMBL	CHEMBL5003.
NextBio	966236.

Entry information

Entry name

CAK1_YEAST

Accession

Primary (citable) accession number: P43568
Secondary accession number(s): D6VTK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program