Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alanine--glyoxylate aminotransferase 1

Gene

AGX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has alanine:glyoxylate aminotransferase activity.2 Publications

Catalytic activityi

L-alanine + glyoxylate = pyruvate + glycine.

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=2.26 mM for L-alanine1 Publication
  2. KM=0.18 mM for glyoxylate1 Publication
  1. Vmax=180 µmol/min/mg enzyme1 Publication

Pathwayi: glycine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glycine from glyoxylate.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine--glyoxylate aminotransferase 1 (AGX1)
This subpathway is part of the pathway glycine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from glyoxylate, the pathway glycine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei354Substrate1

GO - Molecular functioni

  • alanine-glyoxylate transaminase activity Source: SGD

GO - Biological processi

  • glycine biosynthetic process, by transamination of glyoxylate Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-372.
YEAST:MONOMER3O-372.
BRENDAi2.6.1.44. 984.
ReactomeiR-SCE-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKP43567.
UniPathwayiUPA00288; UER00428.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--glyoxylate aminotransferase 1 (EC:2.6.1.44)
Gene namesi
Name:AGX1
Ordered Locus Names:YFL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL030W.
SGDiS000001864. AGX1.

Pathology & Biotechi

Disruption phenotypei

Alanine:glyoxylate aminotransferase activity is reduced by 98% relative to wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001502391 – 385Alanine--glyoxylate aminotransferase 1Add BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201N6-(pyridoxal phosphate)lysine1

Proteomic databases

MaxQBiP43567.
PRIDEiP43567.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi31116. 18 interactors.
DIPiDIP-5433N.
IntActiP43567. 1 interactor.
MINTiMINT-513813.

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 13Combined sources4
Helixi18 – 22Combined sources5
Helixi34 – 50Combined sources17
Helixi55 – 57Combined sources3
Beta strandi60 – 66Combined sources7
Helixi70 – 79Combined sources10
Beta strandi88 – 92Combined sources5
Helixi96 – 107Combined sources12
Beta strandi111 – 115Combined sources5
Helixi126 – 135Combined sources10
Beta strandi139 – 147Combined sources9
Turni148 – 151Combined sources4
Helixi156 – 166Combined sources11
Beta strandi170 – 175Combined sources6
Turni177 – 182Combined sources6
Turni187 – 191Combined sources5
Beta strandi193 – 201Combined sources9
Beta strandi209 – 214Combined sources6
Helixi216 – 222Combined sources7
Helixi225 – 228Combined sources4
Helixi238 – 249Combined sources12
Helixi261 – 277Combined sources17
Helixi279 – 298Combined sources20
Turni299 – 301Combined sources3
Beta strandi304 – 307Combined sources4
Beta strandi309 – 311Combined sources3
Beta strandi319 – 322Combined sources4
Helixi326 – 335Combined sources10
Turni346 – 348Combined sources3
Helixi349 – 351Combined sources3
Beta strandi352 – 355Combined sources4
Helixi359 – 361Combined sources3
Helixi369 – 380Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BKWX-ray2.57A1-385[»]
ProteinModelPortaliP43567.
SMRiP43567.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43567.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171814.
InParanoidiP43567.
KOiK00830.
OMAiDTMIYNY.
OrthoDBiEOG092C33EN.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSVDTLLI PGPIILSGAV QKALDVPSLG HTSPEFVSIF QRVLKNTRAV
60 70 80 90 100
FKSAAASKSQ PFVLAGSGTL GWDIFASNFI LSKAPNKNVL VVSTGTFSDR
110 120 130 140 150
FADCLRSYGA QVDVVRPLKI GESVPLELIT EKLSQNSYGA VTVTHVDTST
160 170 180 190 200
AVLSDLKAIS QAIKQTSPET FFVVDAVCSI GCEEFEFDEW GVDFALTASQ
210 220 230 240 250
KAIGAPAGLS ISLCSSRFMD YALNDSKNGH VHGYFSSLRR WTPIMENYEA
260 270 280 290 300
GKGAYFATPP VQLINSLDVA LKEILEEGLH KRWDLHREMS DWFKDSLVNG
310 320 330 340 350
LQLTSVSRYP SNMSAHGLTA VYVADPPDVI AFLKSHGVVI AGGIHKDIGP
360 370 380
KYIRIGHMGV TACNKNLPYM KNCFDLIKLA LQRKK
Length:385
Mass (Da):41,907
Last modified:November 1, 1995 - v1
Checksum:i460D5DCCA8FDF79F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09208.1.
BK006940 Genomic DNA. Translation: DAA12410.1.
PIRiS56224.
RefSeqiNP_116623.1. NM_001179936.1.

Genome annotation databases

EnsemblFungiiBAA09208; BAA09208; BAA09208.
YFL030W; YFL030W; YFL030W.
GeneIDi850514.
KEGGisce:YFL030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09208.1.
BK006940 Genomic DNA. Translation: DAA12410.1.
PIRiS56224.
RefSeqiNP_116623.1. NM_001179936.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BKWX-ray2.57A1-385[»]
ProteinModelPortaliP43567.
SMRiP43567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31116. 18 interactors.
DIPiDIP-5433N.
IntActiP43567. 1 interactor.
MINTiMINT-513813.

Proteomic databases

MaxQBiP43567.
PRIDEiP43567.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09208; BAA09208; BAA09208.
YFL030W; YFL030W; YFL030W.
GeneIDi850514.
KEGGisce:YFL030W.

Organism-specific databases

EuPathDBiFungiDB:YFL030W.
SGDiS000001864. AGX1.

Phylogenomic databases

GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171814.
InParanoidiP43567.
KOiK00830.
OMAiDTMIYNY.
OrthoDBiEOG092C33EN.

Enzyme and pathway databases

UniPathwayiUPA00288; UER00428.
BioCyciMetaCyc:MONOMER3O-372.
YEAST:MONOMER3O-372.
BRENDAi2.6.1.44. 984.
ReactomeiR-SCE-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKP43567.

Miscellaneous databases

EvolutionaryTraceiP43567.
PROiP43567.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGX1_YEAST
AccessioniPrimary (citable) accession number: P43567
Secondary accession number(s): D6VTK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 339 molecules/cell in log phase SD medium. Expression levels higher in stationary phase than in exponential growth phase when grown in complex medium with glucose.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.