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P43567

- AGX1_YEAST

UniProt

P43567 - AGX1_YEAST

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Protein

Alanine--glyoxylate aminotransferase 1

Gene

AGX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has alanine:glyoxylate aminotransferase activity.2 Publications

Catalytic activityi

L-alanine + glyoxylate = pyruvate + glycine.

Cofactori

Pyridoxal phosphate.1 Publication

Kineticsi

  1. KM=2.26 mM for L-alanine1 Publication
  2. KM=0.18 mM for glyoxylate1 Publication

Vmax=180 µmol/min/mg enzyme1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei354 – 3541Substrate

GO - Molecular functioni

  1. alanine-glyoxylate transaminase activity Source: SGD
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process, by transamination of glyoxylate Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13656.
YEAST:MONOMER3O-372.
BRENDAi2.6.1.44. 984.
SABIO-RKP43567.
UniPathwayiUPA00288; UER00428.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--glyoxylate aminotransferase 1 (EC:2.6.1.44)
Gene namesi
Name:AGX1
Ordered Locus Names:YFL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL030w.
SGDiS000001864. AGX1.

Pathology & Biotechi

Disruption phenotypei

Alanine:glyoxylate aminotransferase activity is reduced by 98% relative to wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Alanine--glyoxylate aminotransferase 1PRO_0000150239Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011N6-(pyridoxal phosphate)lysine

Proteomic databases

MaxQBiP43567.
PaxDbiP43567.
PeptideAtlasiP43567.

Expressioni

Gene expression databases

GenevestigatoriP43567.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi31116. 17 interactions.
DIPiDIP-5433N.
MINTiMINT-513813.
STRINGi4932.YFL030W.

Structurei

Secondary structure

1
385
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 134
Helixi18 – 225
Helixi34 – 5017
Helixi55 – 573
Beta strandi60 – 667
Helixi70 – 7910
Beta strandi88 – 925
Helixi96 – 10712
Beta strandi111 – 1155
Helixi126 – 13510
Beta strandi139 – 1479
Turni148 – 1514
Helixi156 – 16611
Beta strandi170 – 1756
Turni177 – 1826
Turni187 – 1915
Beta strandi193 – 2019
Beta strandi209 – 2146
Helixi216 – 2227
Helixi225 – 2284
Helixi238 – 24912
Helixi261 – 27717
Helixi279 – 29820
Turni299 – 3013
Beta strandi304 – 3074
Beta strandi309 – 3113
Beta strandi319 – 3224
Helixi326 – 33510
Turni346 – 3483
Helixi349 – 3513
Beta strandi352 – 3554
Helixi359 – 3613
Helixi369 – 38012

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BKWX-ray2.57A1-385[»]
ProteinModelPortaliP43567.
SMRiP43567. Positions 3-383.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43567.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0075.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171814.
InParanoidiP43567.
KOiK00830.
OMAiAMARPTI.
OrthoDBiEOG7BZW2X.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43567 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKSVDTLLI PGPIILSGAV QKALDVPSLG HTSPEFVSIF QRVLKNTRAV
60 70 80 90 100
FKSAAASKSQ PFVLAGSGTL GWDIFASNFI LSKAPNKNVL VVSTGTFSDR
110 120 130 140 150
FADCLRSYGA QVDVVRPLKI GESVPLELIT EKLSQNSYGA VTVTHVDTST
160 170 180 190 200
AVLSDLKAIS QAIKQTSPET FFVVDAVCSI GCEEFEFDEW GVDFALTASQ
210 220 230 240 250
KAIGAPAGLS ISLCSSRFMD YALNDSKNGH VHGYFSSLRR WTPIMENYEA
260 270 280 290 300
GKGAYFATPP VQLINSLDVA LKEILEEGLH KRWDLHREMS DWFKDSLVNG
310 320 330 340 350
LQLTSVSRYP SNMSAHGLTA VYVADPPDVI AFLKSHGVVI AGGIHKDIGP
360 370 380
KYIRIGHMGV TACNKNLPYM KNCFDLIKLA LQRKK
Length:385
Mass (Da):41,907
Last modified:November 1, 1995 - v1
Checksum:i460D5DCCA8FDF79F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50617 Genomic DNA. Translation: BAA09208.1.
BK006940 Genomic DNA. Translation: DAA12410.1.
PIRiS56224.
RefSeqiNP_116623.1. NM_001179936.1.

Genome annotation databases

EnsemblFungiiYFL030W; YFL030W; YFL030W.
GeneIDi850514.
KEGGisce:YFL030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D50617 Genomic DNA. Translation: BAA09208.1 .
BK006940 Genomic DNA. Translation: DAA12410.1 .
PIRi S56224.
RefSeqi NP_116623.1. NM_001179936.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2BKW X-ray 2.57 A 1-385 [» ]
ProteinModelPortali P43567.
SMRi P43567. Positions 3-383.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31116. 17 interactions.
DIPi DIP-5433N.
MINTi MINT-513813.
STRINGi 4932.YFL030W.

Proteomic databases

MaxQBi P43567.
PaxDbi P43567.
PeptideAtlasi P43567.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL030W ; YFL030W ; YFL030W .
GeneIDi 850514.
KEGGi sce:YFL030W.

Organism-specific databases

CYGDi YFL030w.
SGDi S000001864. AGX1.

Phylogenomic databases

eggNOGi COG0075.
GeneTreei ENSGT00390000006648.
HOGENOMi HOG000171814.
InParanoidi P43567.
KOi K00830.
OMAi AMARPTI.
OrthoDBi EOG7BZW2X.

Enzyme and pathway databases

UniPathwayi UPA00288 ; UER00428 .
BioCyci MetaCyc:MONOMER-13656.
YEAST:MONOMER3O-372.
BRENDAi 2.6.1.44. 984.
SABIO-RK P43567.

Miscellaneous databases

EvolutionaryTracei P43567.
NextBioi 966233.
PROi P43567.

Gene expression databases

Genevestigatori P43567.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR000192. Aminotrans_V_dom.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view ]
Pfami PF00266. Aminotran_5. 1 hit.
[Graphical view ]
PIRSFi PIRSF000524. SPT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Characteristics of alanine:glyoxylate aminotransferase from Saccharomyces cerevisiae, a regulatory enzyme in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates."
    Takada Y., Noguchi T.
    Biochem. J. 231:157-163(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Alanine:glyoxylate aminotransferase of Saccharomyces cerevisiae-encoding gene AGX1 and metabolic significance."
    Schlosser T., Gatgens C., Weber U., Stahmann K.-P.
    Yeast 21:63-73(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Crystal structure and confirmation of the alanine:glyoxylate aminotransferase activity of the YFL030w yeast protein."
    Meyer P., Liger D., Leulliot N., Quevillon-Cheruel S., Zhou C.-Z., Borel F., Ferrer J.-L., Poupon A., Janin J., van Tilbeurgh H.
    Biochimie 87:1041-1047(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS) IN COMPLEX WITH PYRODOXAL PHOSPHATE AND GLYOXYLATE, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, DISRUPTION PHENOTYPE, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiAGX1_YEAST
AccessioniPrimary (citable) accession number: P43567
Secondary accession number(s): D6VTK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 339 molecules/cell in log phase SD medium. Expression levels higher in stationary phase than in exponential growth phase when grown in complex medium with glucose.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3