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P43567

- AGX1_YEAST

UniProt

P43567 - AGX1_YEAST

Protein

Alanine--glyoxylate aminotransferase 1

Gene

AGX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Has alanine:glyoxylate aminotransferase activity.2 Publications

    Catalytic activityi

    L-alanine + glyoxylate = pyruvate + glycine.

    Cofactori

    Pyridoxal phosphate.1 Publication

    Kineticsi

    1. KM=2.26 mM for L-alanine1 Publication
    2. KM=0.18 mM for glyoxylate1 Publication

    Vmax=180 µmol/min/mg enzyme1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei354 – 3541Substrate

    GO - Molecular functioni

    1. alanine-glyoxylate transaminase activity Source: SGD
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. glycine biosynthetic process, by transamination of glyoxylate Source: SGD

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13656.
    YEAST:MONOMER3O-372.
    BRENDAi2.6.1.44. 984.
    SABIO-RKP43567.
    UniPathwayiUPA00288; UER00428.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine--glyoxylate aminotransferase 1 (EC:2.6.1.44)
    Gene namesi
    Name:AGX1
    Ordered Locus Names:YFL030W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL030w.
    SGDiS000001864. AGX1.

    Pathology & Biotechi

    Disruption phenotypei

    Alanine:glyoxylate aminotransferase activity is reduced by 98% relative to wild-type.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 385385Alanine--glyoxylate aminotransferase 1PRO_0000150239Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei201 – 2011N6-(pyridoxal phosphate)lysine

    Proteomic databases

    MaxQBiP43567.
    PaxDbiP43567.
    PeptideAtlasiP43567.

    Expressioni

    Gene expression databases

    GenevestigatoriP43567.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi31116. 17 interactions.
    DIPiDIP-5433N.
    MINTiMINT-513813.
    STRINGi4932.YFL030W.

    Structurei

    Secondary structure

    1
    385
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 134
    Helixi18 – 225
    Helixi34 – 5017
    Helixi55 – 573
    Beta strandi60 – 667
    Helixi70 – 7910
    Beta strandi88 – 925
    Helixi96 – 10712
    Beta strandi111 – 1155
    Helixi126 – 13510
    Beta strandi139 – 1479
    Turni148 – 1514
    Helixi156 – 16611
    Beta strandi170 – 1756
    Turni177 – 1826
    Turni187 – 1915
    Beta strandi193 – 2019
    Beta strandi209 – 2146
    Helixi216 – 2227
    Helixi225 – 2284
    Helixi238 – 24912
    Helixi261 – 27717
    Helixi279 – 29820
    Turni299 – 3013
    Beta strandi304 – 3074
    Beta strandi309 – 3113
    Beta strandi319 – 3224
    Helixi326 – 33510
    Turni346 – 3483
    Helixi349 – 3513
    Beta strandi352 – 3554
    Helixi359 – 3613
    Helixi369 – 38012

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2BKWX-ray2.57A1-385[»]
    ProteinModelPortaliP43567.
    SMRiP43567. Positions 3-383.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43567.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0075.
    GeneTreeiENSGT00390000006648.
    HOGENOMiHOG000171814.
    KOiK00830.
    OMAiAMARPTI.
    OrthoDBiEOG7BZW2X.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000192. Aminotrans_V/Cys_dSase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR024169. SP_NH2Trfase/AEP_transaminase.
    [Graphical view]
    PfamiPF00266. Aminotran_5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000524. SPT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43567-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKSVDTLLI PGPIILSGAV QKALDVPSLG HTSPEFVSIF QRVLKNTRAV    50
    FKSAAASKSQ PFVLAGSGTL GWDIFASNFI LSKAPNKNVL VVSTGTFSDR 100
    FADCLRSYGA QVDVVRPLKI GESVPLELIT EKLSQNSYGA VTVTHVDTST 150
    AVLSDLKAIS QAIKQTSPET FFVVDAVCSI GCEEFEFDEW GVDFALTASQ 200
    KAIGAPAGLS ISLCSSRFMD YALNDSKNGH VHGYFSSLRR WTPIMENYEA 250
    GKGAYFATPP VQLINSLDVA LKEILEEGLH KRWDLHREMS DWFKDSLVNG 300
    LQLTSVSRYP SNMSAHGLTA VYVADPPDVI AFLKSHGVVI AGGIHKDIGP 350
    KYIRIGHMGV TACNKNLPYM KNCFDLIKLA LQRKK 385
    Length:385
    Mass (Da):41,907
    Last modified:November 1, 1995 - v1
    Checksum:i460D5DCCA8FDF79F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09208.1.
    BK006940 Genomic DNA. Translation: DAA12410.1.
    PIRiS56224.
    RefSeqiNP_116623.1. NM_001179936.1.

    Genome annotation databases

    EnsemblFungiiYFL030W; YFL030W; YFL030W.
    GeneIDi850514.
    KEGGisce:YFL030W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09208.1 .
    BK006940 Genomic DNA. Translation: DAA12410.1 .
    PIRi S56224.
    RefSeqi NP_116623.1. NM_001179936.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2BKW X-ray 2.57 A 1-385 [» ]
    ProteinModelPortali P43567.
    SMRi P43567. Positions 3-383.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31116. 17 interactions.
    DIPi DIP-5433N.
    MINTi MINT-513813.
    STRINGi 4932.YFL030W.

    Proteomic databases

    MaxQBi P43567.
    PaxDbi P43567.
    PeptideAtlasi P43567.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL030W ; YFL030W ; YFL030W .
    GeneIDi 850514.
    KEGGi sce:YFL030W.

    Organism-specific databases

    CYGDi YFL030w.
    SGDi S000001864. AGX1.

    Phylogenomic databases

    eggNOGi COG0075.
    GeneTreei ENSGT00390000006648.
    HOGENOMi HOG000171814.
    KOi K00830.
    OMAi AMARPTI.
    OrthoDBi EOG7BZW2X.

    Enzyme and pathway databases

    UniPathwayi UPA00288 ; UER00428 .
    BioCyci MetaCyc:MONOMER-13656.
    YEAST:MONOMER3O-372.
    BRENDAi 2.6.1.44. 984.
    SABIO-RK P43567.

    Miscellaneous databases

    EvolutionaryTracei P43567.
    NextBioi 966233.
    PROi P43567.

    Gene expression databases

    Genevestigatori P43567.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR000192. Aminotrans_V/Cys_dSase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR024169. SP_NH2Trfase/AEP_transaminase.
    [Graphical view ]
    Pfami PF00266. Aminotran_5. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000524. SPT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Characteristics of alanine:glyoxylate aminotransferase from Saccharomyces cerevisiae, a regulatory enzyme in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates."
      Takada Y., Noguchi T.
      Biochem. J. 231:157-163(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Alanine:glyoxylate aminotransferase of Saccharomyces cerevisiae-encoding gene AGX1 and metabolic significance."
      Schlosser T., Gatgens C., Weber U., Stahmann K.-P.
      Yeast 21:63-73(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Crystal structure and confirmation of the alanine:glyoxylate aminotransferase activity of the YFL030w yeast protein."
      Meyer P., Liger D., Leulliot N., Quevillon-Cheruel S., Zhou C.-Z., Borel F., Ferrer J.-L., Poupon A., Janin J., van Tilbeurgh H.
      Biochimie 87:1041-1047(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS) IN COMPLEX WITH PYRODOXAL PHOSPHATE AND GLYOXYLATE, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, DISRUPTION PHENOTYPE, FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiAGX1_YEAST
    AccessioniPrimary (citable) accession number: P43567
    Secondary accession number(s): D6VTK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 339 molecules/cell in log phase SD medium. Expression levels higher in stationary phase than in exponential growth phase when grown in complex medium with glucose.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3