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Protein

Alanine--glyoxylate aminotransferase 1

Gene

AGX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has alanine:glyoxylate aminotransferase activity.2 Publications

Catalytic activityi

L-alanine + glyoxylate = pyruvate + glycine.

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=2.26 mM for L-alanine1 Publication
  2. KM=0.18 mM for glyoxylate1 Publication

Vmax=180 µmol/min/mg enzyme1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei354 – 3541Substrate

GO - Molecular functioni

  1. alanine-glyoxylate transaminase activity Source: SGD
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process, by transamination of glyoxylate Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13656.
YEAST:MONOMER3O-372.
BRENDAi2.6.1.44. 984.
ReactomeiREACT_246681. Glyoxylate metabolism.
SABIO-RKP43567.
UniPathwayiUPA00288; UER00428.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--glyoxylate aminotransferase 1 (EC:2.6.1.44)
Gene namesi
Name:AGX1
Ordered Locus Names:YFL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL030w.
SGDiS000001864. AGX1.

Pathology & Biotechi

Disruption phenotypei

Alanine:glyoxylate aminotransferase activity is reduced by 98% relative to wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Alanine--glyoxylate aminotransferase 1PRO_0000150239Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011N6-(pyridoxal phosphate)lysine

Proteomic databases

MaxQBiP43567.
PaxDbiP43567.
PeptideAtlasiP43567.

Expressioni

Gene expression databases

GenevestigatoriP43567.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi31116. 17 interactions.
DIPiDIP-5433N.
MINTiMINT-513813.
STRINGi4932.YFL030W.

Structurei

Secondary structure

1
385
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 134Combined sources
Helixi18 – 225Combined sources
Helixi34 – 5017Combined sources
Helixi55 – 573Combined sources
Beta strandi60 – 667Combined sources
Helixi70 – 7910Combined sources
Beta strandi88 – 925Combined sources
Helixi96 – 10712Combined sources
Beta strandi111 – 1155Combined sources
Helixi126 – 13510Combined sources
Beta strandi139 – 1479Combined sources
Turni148 – 1514Combined sources
Helixi156 – 16611Combined sources
Beta strandi170 – 1756Combined sources
Turni177 – 1826Combined sources
Turni187 – 1915Combined sources
Beta strandi193 – 2019Combined sources
Beta strandi209 – 2146Combined sources
Helixi216 – 2227Combined sources
Helixi225 – 2284Combined sources
Helixi238 – 24912Combined sources
Helixi261 – 27717Combined sources
Helixi279 – 29820Combined sources
Turni299 – 3013Combined sources
Beta strandi304 – 3074Combined sources
Beta strandi309 – 3113Combined sources
Beta strandi319 – 3224Combined sources
Helixi326 – 33510Combined sources
Turni346 – 3483Combined sources
Helixi349 – 3513Combined sources
Beta strandi352 – 3554Combined sources
Helixi359 – 3613Combined sources
Helixi369 – 38012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BKWX-ray2.57A1-385[»]
ProteinModelPortaliP43567.
SMRiP43567. Positions 3-383.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43567.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0075.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171814.
InParanoidiP43567.
KOiK00830.
OMAiAFLCEKP.
OrthoDBiEOG7BZW2X.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43567-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKSVDTLLI PGPIILSGAV QKALDVPSLG HTSPEFVSIF QRVLKNTRAV
60 70 80 90 100
FKSAAASKSQ PFVLAGSGTL GWDIFASNFI LSKAPNKNVL VVSTGTFSDR
110 120 130 140 150
FADCLRSYGA QVDVVRPLKI GESVPLELIT EKLSQNSYGA VTVTHVDTST
160 170 180 190 200
AVLSDLKAIS QAIKQTSPET FFVVDAVCSI GCEEFEFDEW GVDFALTASQ
210 220 230 240 250
KAIGAPAGLS ISLCSSRFMD YALNDSKNGH VHGYFSSLRR WTPIMENYEA
260 270 280 290 300
GKGAYFATPP VQLINSLDVA LKEILEEGLH KRWDLHREMS DWFKDSLVNG
310 320 330 340 350
LQLTSVSRYP SNMSAHGLTA VYVADPPDVI AFLKSHGVVI AGGIHKDIGP
360 370 380
KYIRIGHMGV TACNKNLPYM KNCFDLIKLA LQRKK
Length:385
Mass (Da):41,907
Last modified:November 1, 1995 - v1
Checksum:i460D5DCCA8FDF79F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09208.1.
BK006940 Genomic DNA. Translation: DAA12410.1.
PIRiS56224.
RefSeqiNP_116623.1. NM_001179936.1.

Genome annotation databases

EnsemblFungiiYFL030W; YFL030W; YFL030W.
GeneIDi850514.
KEGGisce:YFL030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09208.1.
BK006940 Genomic DNA. Translation: DAA12410.1.
PIRiS56224.
RefSeqiNP_116623.1. NM_001179936.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BKWX-ray2.57A1-385[»]
ProteinModelPortaliP43567.
SMRiP43567. Positions 3-383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31116. 17 interactions.
DIPiDIP-5433N.
MINTiMINT-513813.
STRINGi4932.YFL030W.

Proteomic databases

MaxQBiP43567.
PaxDbiP43567.
PeptideAtlasiP43567.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYFL030W; YFL030W; YFL030W.
GeneIDi850514.
KEGGisce:YFL030W.

Organism-specific databases

CYGDiYFL030w.
SGDiS000001864. AGX1.

Phylogenomic databases

eggNOGiCOG0075.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171814.
InParanoidiP43567.
KOiK00830.
OMAiAFLCEKP.
OrthoDBiEOG7BZW2X.

Enzyme and pathway databases

UniPathwayiUPA00288; UER00428.
BioCyciMetaCyc:MONOMER-13656.
YEAST:MONOMER3O-372.
BRENDAi2.6.1.44. 984.
ReactomeiREACT_246681. Glyoxylate metabolism.
SABIO-RKP43567.

Miscellaneous databases

EvolutionaryTraceiP43567.
NextBioi966233.
PROiP43567.

Gene expression databases

GenevestigatoriP43567.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Characteristics of alanine:glyoxylate aminotransferase from Saccharomyces cerevisiae, a regulatory enzyme in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates."
    Takada Y., Noguchi T.
    Biochem. J. 231:157-163(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Alanine:glyoxylate aminotransferase of Saccharomyces cerevisiae-encoding gene AGX1 and metabolic significance."
    Schlosser T., Gatgens C., Weber U., Stahmann K.-P.
    Yeast 21:63-73(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Crystal structure and confirmation of the alanine:glyoxylate aminotransferase activity of the YFL030w yeast protein."
    Meyer P., Liger D., Leulliot N., Quevillon-Cheruel S., Zhou C.-Z., Borel F., Ferrer J.-L., Poupon A., Janin J., van Tilbeurgh H.
    Biochimie 87:1041-1047(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS) IN COMPLEX WITH PYRODOXAL PHOSPHATE AND GLYOXYLATE, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, DISRUPTION PHENOTYPE, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiAGX1_YEAST
AccessioniPrimary (citable) accession number: P43567
Secondary accession number(s): D6VTK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 339 molecules/cell in log phase SD medium. Expression levels higher in stationary phase than in exponential growth phase when grown in complex medium with glucose.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.