P43564 (YFD4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized membrane protein YFL034W

Gene names

Ordered Locus Names:

YFL034W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1073 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with RPP0. Ref.4
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 1110 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the TMCO4 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GDE1	Q02979	1	EBI-22823,EBI-31287
MYO1	P08964	1	EBI-22823,EBI-11650

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1073	1073	Uncharacterized membrane protein YFL034W		PRO_0000202674
Regions
	Topological domain	1 – 552	552	Cytoplasmic Potential
	Transmembrane	553 – 573	21	Helical; Potential
	Topological domain	574 – 576	3	Extracellular Potential
	Transmembrane	577 – 597	21	Helical; Potential
	Topological domain	598 – 694	97	Cytoplasmic Potential
	Transmembrane	695 – 715	21	Helical; Potential
	Topological domain	716 – 752	37	Extracellular Potential
	Transmembrane	753 – 773	21	Helical; Potential
	Topological domain	774 – 1073	300	Cytoplasmic Potential
Amino acid modifications
	Modified residue	94	1	Phosphoserine Ref.6
	Modified residue	211	1	Phosphothreonine Ref.6
	Modified residue	215	1	Phosphothreonine Ref.6
	Modified residue	435	1	Phosphoserine Ref.6
	Modified residue	1037	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	323	1	K → N in BAA09205. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P43564 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: 2CB2CAB4F9E24DA2	FASTA	1,073	119,510
	10 20 30 40 50 60 MSDSEEDLGV QLKGLKIARH LKESGEHTDE ESNSSPEHDC GLSNQDDLTV MHTQAKEEVF 70 80 90 100 110 120 KRREEDGTRT EDALHEGEAG KEGTGFPSSQ SVCSPNEADS GIDRADKPIL LDPFKSVHDT 130 140 150 160 170 180 DPVPGTKSRS NSDSDSDSDD GGWQEMPAVS SFNIYNHRGE LELTSKVRNS EQASETSPTV 190 200 210 220 230 240 PPGKNCKSVN DSRFDYTKMA AEQQAQRSYR TNKKTDFLFD HKVLKKKINS SQTSVNLTSS 250 260 270 280 290 300 PSTTSLNNEK NNDDDDDDSY DEYEDDVEPV NDLNRDSQLN ITKNLLSDME KFAYVGAINI 310 320 330 340 350 360 LANQMCTNLA TLCLCIDIKS HKKLAHRLQF TQKDMAAWKT VVLSRLYDHL GISQEEIVMI 370 380 390 400 410 420 EKLSLHKIQL EDLCKCLKTT QSIDNPWEND RDHEEDGIEE TTERMSPNEQ NGSVQASTPD 430 440 450 460 470 480 PEQSATPETP KAKQSPLSSD VPGKVLDPEN VKSQDKLNID VAWTIICDLF LICLQSSTYD 490 500 510 520 530 540 SRSRTLLINF AKVLNMTSLE ICEFERRVTD SLDMEQSTED QVWDEQDHMR NRRRSKRRKK 550 560 570 580 590 600 MAYVALAMVG GSLVLGLSGG LLAPVIGGGI AAGLSTIGIT GATSFLTGVG GTTVVAVSST 610 620 630 640 650 660 AIGANIGARG MSKRMGSVRT FEFRPLHNNR RVNLILTVSG WMVGNEDDVR LPFSTVDPVE 670 680 690 700 710 720 GDLYSLYWEP EMLKSIGQTV SIVATEIFTT SLQQILGATV LTALISSIQW PMALSKLGYI 730 740 750 760 770 780 LDNPWNVSLD RAWSAGKILA DTLIARNLGA RPITLVGFSI GARVIFSCLI ELCKKKALGL 790 800 810 820 830 840 IENVYLFGTP AVMKKEQLVM ARSVVSGRFV NGYSDKDWFL AYLFRAAAGG FSAVMGISTI 850 860 870 880 890 900 ENVEGIENIN CTEFVDGHLN YRKSMPKLLK RIGIAVLSEE FVEIEEMMNP EEVKRKRKLI 910 920 930 940 950 960 NDVDAAQKKL SERKKHNSWV PKWLKPKKSK WKVMVEEAVE EGRDMQDLPE NDVNNNENEN 970 980 990 1000 1010 1020 PDEHEGIARQ KRRDAALVDH GALMHELQLI KQAMHEDEIK NKACLPGEDK EVESSNDFLG 1030 1040 1050 1060 1070 ESHYKPPSTP KINPPQSPNN FQLLSAGRTI LPEDDDFDPR GKKKVEFSFP DDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 323. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Identification of a hypothetical membrane protein interactor of ribosomal phosphoprotein P0." Aruna K., Chakraborty T., Nambeesan S., Mannan A.B., Sehgal A., Balachandara S.R., Sharma S. J. Biosci. 29:33-43(2004) [PubMed: 15286401] [Abstract] Cited for: INTERACTION WITH RPP0.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94; THR-211; THR-215; SER-435 AND SER-1037, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09205.1. BK006940 Genomic DNA. Translation: DAA12407.2.
PIR	S56220.
RefSeq	NP_116583.2. NM_001179932.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6508N.
IntAct	P43564. 4 interactions.
MINT	MINT-706942.
STRING	P43564.
Proteomic databases
PeptideAtlas	P43564.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	850510.
KEGG	sce:YFL034W.
NMPDR	fig|4932.3.peg.2245.
Organism-specific databases
CYGD	YFL034w.
SGD	S000001860. YFL034W.
Phylogenomic databases
eggNOG	fuNOG05197.
GeneTree	EFGT00050000003007.
HOGENOM	HBG397864.
OMA	MSAIQLP.
OrthoDB	EOG4QJVWS.
Gene expression databases
ArrayExpress	P43564.
Genevestigator	P43564.
GermOnline	YFL034W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007941. DUF726. [Graphical view]
PANTHER	PTHR17920. DUF726. 1 hit.
Pfam	PF05277. DUF726. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966224.

Entry information

Entry name

YFD4_YEAST

Accession

Primary (citable) accession number: P43564
Secondary accession number(s): D6VTJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	September 21, 2011
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program