P43563 (MOB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CBK1 kinase activator protein MOB2

Alternative name(s):
MPS1 binder 2
Maintenance of ploidy protein MOB2

Gene names

Name:	MOB2
Ordered Locus Names:	YFL034C-B
ORF Names:	YFL035C, YFL035C-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as an activator subunit for the CBK1 protein kinase. Part of the regulation of ACE2 activity and cellular morphogenesis (RAM) signaling network. Required for coordinating polarized cell growth during interphase with the onset of mitosis. Required for mother/daughter cell separation after cytokinesis. Also has a role in the prevention of nuclear export of ACE2 from the daughter cell nucleus after mitotic exit. It coordinates ACE2-dependent transcription with mitotic exit network activation. Ref.4 Ref.5
Subunit structure	Interacts with protein kinase CBK1 to form the RAM CBK1-MOB2 kinase complex. Ref.5 Ref.7
Subcellular location	Nucleus. Cytoplasm. Note: Localizes to the bud cortex during polarized growth and to the bud neck and daughter cell nucleus during late mitosis. Ref.4 Ref.5
Miscellaneous	Present with 2250 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the MOB1/phocein family.
Sequence caution	The sequence BAA09204.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	budding cell apical bud growth Inferred from mutant phenotype. Source: SGD cytokinetic cell separation involved in cell cycle cytokinesis Inferred from mutant phenotype Ref.4Ref.5. Source: SGD establishment or maintenance of cell polarity Inferred from mutant phenotype Ref.5. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cellular bud neck Inferred from direct assay Ref.4Ref.5. Source: SGD cellular bud tip Inferred from direct assay Ref.4. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell mating projection tip Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay Ref.4Ref.5. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein kinase activator activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CBK1	P53894	5	EBI-11125,EBI-4110

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	CBK1 kinase activator protein MOB2		PRO_0000193583

Sequences

	Sequence		Length	Mass (Da)
	P43563 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: CFBA15FF83BFAA33	FASTA	287	33,276
	10 20 30 40 50 60 MSFFNFKAFG RNSKKNKNQP LNVAQPPAMN TIYSSPHSSN SRLSLRNKHH SPKRHSQTSF 70 80 90 100 110 120 PAQKSTPQSQ QLTSTTPQSQ QQEASERSES QQIMFLSEPF VRTALVKGSF KTIVQLPKYV 130 140 150 160 170 180 DLGEWIALNV FEFFTNLNQF YGVVAEYVTP DAYPTMNAGP HTDYLWLDAN NRQVSLPASQ 190 200 210 220 230 240 YIDLALTWIN NKVNDKNLFP TKNGLPFPQQ FSRDVQRIMV QMFRIFAHIY HHHFDKIVHL 250 260 270 280 SLEAHWNSFF SHFISFAKEF KIIDRKEMAP LLPLIESFEK QGKIIYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy." Luca F.C., Winey M. Mol. Biol. Cell 9:29-46(1998) [PubMed: 9436989] [Abstract] Cited for: IDENTIFICATION OF INTRON.
[4]	"Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates." Colman-Lerner A., Chin T.E., Brent R. Cell 107:739-750(2001) [PubMed: 11747810] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor." Weiss E.L., Kurischko C., Zhang C., Shokat K., Drubin D.G., Luca F.C. J. Cell Biol. 158:885-900(2002) [PubMed: 12196508] [Abstract] Cited for: FUNCTION, INTERACTION WITH CBK1, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Structural and functional analysis of Saccharomyces cerevisiae Mob1." Mrkobrada S., Boucher L., Ceccarelli D.F.J., Tyers M., Sicheri F. J. Mol. Biol. 362:430-440(2006) [PubMed: 16934835] [Abstract] Cited for: INTERACTION WITH MOB1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09204.1. Sequence problems. BK006940 Genomic DNA. Translation: DAA12405.1.
PIR	S58648.
RefSeq	NP_116618.1. NM_001179931.1.
3D structure databases
ProteinModelPortal	P43563.
SMR	P43563. Positions 95-283.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1449N.
IntAct	P43563. 13 interactions.
MINT	MINT-391527.
STRING	P43563.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL034C-B; YFL034C-B; YFL034C-B.
GeneID	850508.
KEGG	sce:YFL034C-B.
NMPDR	fig|4932.3.peg.2243.
Organism-specific databases
CYGD	YFL034c-b.
SGD	S000001859. MOB2.
Phylogenomic databases
eggNOG	fuNOG05198.
GeneTree	EFGT00050000002022.
HOGENOM	HBG737456.
OMA	FAHFISF.
OrthoDB	EOG4PP1S4.
Gene expression databases
ArrayExpress	P43563.
Genevestigator	P43563.
GermOnline	YFL034C-B. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005301. Mob1_phocein. [Graphical view]
Gene3D	G3DSA:1.20.140.30. Mob1_phocein. 1 hit.
PANTHER	PTHR22599. Mob1_phocein. 1 hit.
Pfam	PF03637. Mob1_phocein. 1 hit. [Graphical view]
SUPFAM	SSF101152. Mob1_phocein. 1 hit.
ProtoNet	Search...
Other
NextBio	966218.

Entry information

Entry name

MOB2_YEAST

Accession

Primary (citable) accession number: P43563
Secondary accession number(s): D6VTJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 17, 2006
Last modified:	December 14, 2011
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program