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Protein

CBK1 kinase activator protein MOB2

Gene

MOB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an activator subunit for the CBK1 protein kinase. Part of the regulation of ACE2 activity and cellular morphogenesis (RAM) signaling network. Required for coordinating polarized cell growth during interphase with the onset of mitosis. Required for mother/daughter cell separation after cytokinesis. Also has a role in the prevention of nuclear export of ACE2 from the daughter cell nucleus after mitotic exit. It coordinates ACE2-dependent transcription with mitotic exit network activation.2 Publications

GO - Molecular functioni

  • protein kinase activator activity Source: SGD

GO - Biological processi

  • activation of protein kinase activity Source: GOC
  • budding cell apical bud growth Source: SGD
  • cell separation after cytokinesis Source: SGD
  • establishment or maintenance of cell polarity Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of protein phosphorylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CBK1 kinase activator protein MOB2
Alternative name(s):
MPS1 binder 2
Maintenance of ploidy protein MOB2
Gene namesi
Name:MOB2
Ordered Locus Names:YFL034C-B
ORF Names:YFL035C, YFL035C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL034C-B.
SGDiS000001859. MOB2.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Localizes to the bud cortex during polarized growth and to the bud neck and daughter cell nucleus during late mitosis.

GO - Cellular componenti

  • cellular bud Source: SGD
  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytoplasm Source: UniProtKB-SubCell
  • mating projection tip Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287CBK1 kinase activator protein MOB2PRO_0000193583Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331PhosphotyrosineCombined sources
Modified residuei59 – 591PhosphoserineCombined sources
Modified residuei76 – 761PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43563.

PTM databases

iPTMnetiP43563.

Interactioni

Subunit structurei

Interacts with protein kinase CBK1 to form the RAM CBK1-MOB2 kinase complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CBK1P538943EBI-11125,EBI-4110

Protein-protein interaction databases

BioGridi31111. 34 interactions.
DIPiDIP-1449N.
IntActiP43563. 14 interactions.
MINTiMINT-391527.

Structurei

Secondary structure

1
287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni112 – 1143Combined sources
Helixi122 – 14322Combined sources
Helixi183 – 19210Combined sources
Turni196 – 1983Combined sources
Helixi211 – 23121Combined sources
Helixi234 – 2374Combined sources
Helixi238 – 2403Combined sources
Helixi243 – 25917Combined sources
Turni265 – 2717Combined sources
Helixi272 – 2754Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LQPX-ray4.50B46-287[»]
4LQQX-ray3.60B/E46-287[»]
4LQSX-ray3.30B46-287[»]
ProteinModelPortaliP43563.
SMRiP43563. Positions 111-278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MOB1/phocein family.Curated

Phylogenomic databases

GeneTreeiENSGT00770000121489.
HOGENOMiHOG000164685.
InParanoidiP43563.
OMAiMNAGPHT.
OrthoDBiEOG708W97.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. MOB_kinase_act_fam.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SMARTiSM01388. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.

Sequencei

Sequence statusi: Complete.

P43563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFNFKAFG RNSKKNKNQP LNVAQPPAMN TIYSSPHSSN SRLSLRNKHH
60 70 80 90 100
SPKRHSQTSF PAQKSTPQSQ QLTSTTPQSQ QQEASERSES QQIMFLSEPF
110 120 130 140 150
VRTALVKGSF KTIVQLPKYV DLGEWIALNV FEFFTNLNQF YGVVAEYVTP
160 170 180 190 200
DAYPTMNAGP HTDYLWLDAN NRQVSLPASQ YIDLALTWIN NKVNDKNLFP
210 220 230 240 250
TKNGLPFPQQ FSRDVQRIMV QMFRIFAHIY HHHFDKIVHL SLEAHWNSFF
260 270 280
SHFISFAKEF KIIDRKEMAP LLPLIESFEK QGKIIYN
Length:287
Mass (Da):33,276
Last modified:October 17, 2006 - v2
Checksum:iCFBA15FF83BFAA33
GO

Sequence cautioni

The sequence BAA09204.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09204.1. Sequence problems.
BK006940 Genomic DNA. Translation: DAA12405.1.
PIRiS58648.
RefSeqiNP_116618.1. NM_001179931.1.

Genome annotation databases

EnsemblFungiiBAA09204; BAA09204; BAA09204.
YFL034C-B; YFL034C-B; YFL034C-B.
GeneIDi850508.
KEGGisce:YFL034C-B.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09204.1. Sequence problems.
BK006940 Genomic DNA. Translation: DAA12405.1.
PIRiS58648.
RefSeqiNP_116618.1. NM_001179931.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LQPX-ray4.50B46-287[»]
4LQQX-ray3.60B/E46-287[»]
4LQSX-ray3.30B46-287[»]
ProteinModelPortaliP43563.
SMRiP43563. Positions 111-278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31111. 34 interactions.
DIPiDIP-1449N.
IntActiP43563. 14 interactions.
MINTiMINT-391527.

PTM databases

iPTMnetiP43563.

Proteomic databases

MaxQBiP43563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09204; BAA09204; BAA09204.
YFL034C-B; YFL034C-B; YFL034C-B.
GeneIDi850508.
KEGGisce:YFL034C-B.

Organism-specific databases

EuPathDBiFungiDB:YFL034C-B.
SGDiS000001859. MOB2.

Phylogenomic databases

GeneTreeiENSGT00770000121489.
HOGENOMiHOG000164685.
InParanoidiP43563.
OMAiMNAGPHT.
OrthoDBiEOG708W97.

Enzyme and pathway databases

BioCyciYEAST:G3O-30427-MONOMER.

Miscellaneous databases

NextBioi966218.
PROiP43563.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. MOB_kinase_act_fam.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SMARTiSM01388. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy."
    Luca F.C., Winey M.
    Mol. Biol. Cell 9:29-46(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INTRON.
  4. "Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates."
    Colman-Lerner A., Chin T.E., Brent R.
    Cell 107:739-750(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor."
    Weiss E.L., Kurischko C., Zhang C., Shokat K., Drubin D.G., Luca F.C.
    J. Cell Biol. 158:885-900(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CBK1, SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Structural and functional analysis of Saccharomyces cerevisiae Mob1."
    Mrkobrada S., Boucher L., Ceccarelli D.F.J., Tyers M., Sicheri F.
    J. Mol. Biol. 362:430-440(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MOB1.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-33; SER-59 AND THR-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMOB2_YEAST
AccessioniPrimary (citable) accession number: P43563
Secondary accession number(s): D6VTJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 17, 2006
Last modified: May 11, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.