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Protein

CBK1 kinase activator protein MOB2

Gene

MOB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an activator subunit for the CBK1 protein kinase. Part of the regulation of ACE2 activity and cellular morphogenesis (RAM) signaling network. Required for coordinating polarized cell growth during interphase with the onset of mitosis. Required for mother/daughter cell separation after cytokinesis. Also has a role in the prevention of nuclear export of ACE2 from the daughter cell nucleus after mitotic exit. It coordinates ACE2-dependent transcription with mitotic exit network activation.2 Publications

GO - Molecular functioni

  • protein kinase activator activity Source: SGD

GO - Biological processi

  • budding cell apical bud growth Source: SGD
  • cell separation after cytokinesis Source: SGD
  • establishment or maintenance of cell polarity Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of protein phosphorylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CBK1 kinase activator protein MOB2
Alternative name(s):
MPS1 binder 2
Maintenance of ploidy protein MOB2
Gene namesi
Name:MOB2
Ordered Locus Names:YFL034C-B
ORF Names:YFL035C, YFL035C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL034C-B.
SGDiS000001859. MOB2.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Localizes to the bud cortex during polarized growth and to the bud neck and daughter cell nucleus during late mitosis.

GO - Cellular componenti

  • cellular bud Source: SGD
  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytoplasm Source: UniProtKB-SubCell
  • mating projection tip Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001935831 – 287CBK1 kinase activator protein MOB2Add BLAST287

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33PhosphotyrosineCombined sources1
Modified residuei59PhosphoserineCombined sources1
Modified residuei76PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43563.
PRIDEiP43563.

PTM databases

iPTMnetiP43563.

Interactioni

Subunit structurei

Interacts with protein kinase CBK1 to form the RAM CBK1-MOB2 kinase complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CBK1P538943EBI-11125,EBI-4110

Protein-protein interaction databases

BioGridi31111. 34 interactors.
DIPiDIP-1449N.
IntActiP43563. 14 interactors.
MINTiMINT-391527.

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni112 – 114Combined sources3
Helixi122 – 143Combined sources22
Helixi183 – 192Combined sources10
Turni196 – 198Combined sources3
Helixi211 – 231Combined sources21
Helixi234 – 237Combined sources4
Helixi238 – 240Combined sources3
Helixi243 – 259Combined sources17
Turni265 – 271Combined sources7
Helixi272 – 275Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LQPX-ray4.50B46-287[»]
4LQQX-ray3.60B/E46-287[»]
4LQSX-ray3.30B46-287[»]
ProteinModelPortaliP43563.
SMRiP43563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MOB1/phocein family.Curated

Phylogenomic databases

HOGENOMiHOG000164685.
InParanoidiP43563.
OMAiSEPFVRT.
OrthoDBiEOG092C4XVR.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. MOB_kinase_act_fam.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SMARTiSM01388. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.

Sequencei

Sequence statusi: Complete.

P43563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFNFKAFG RNSKKNKNQP LNVAQPPAMN TIYSSPHSSN SRLSLRNKHH
60 70 80 90 100
SPKRHSQTSF PAQKSTPQSQ QLTSTTPQSQ QQEASERSES QQIMFLSEPF
110 120 130 140 150
VRTALVKGSF KTIVQLPKYV DLGEWIALNV FEFFTNLNQF YGVVAEYVTP
160 170 180 190 200
DAYPTMNAGP HTDYLWLDAN NRQVSLPASQ YIDLALTWIN NKVNDKNLFP
210 220 230 240 250
TKNGLPFPQQ FSRDVQRIMV QMFRIFAHIY HHHFDKIVHL SLEAHWNSFF
260 270 280
SHFISFAKEF KIIDRKEMAP LLPLIESFEK QGKIIYN
Length:287
Mass (Da):33,276
Last modified:October 17, 2006 - v2
Checksum:iCFBA15FF83BFAA33
GO

Sequence cautioni

The sequence BAA09204 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09204.1. Sequence problems.
BK006940 Genomic DNA. Translation: DAA12405.1.
PIRiS58648.
RefSeqiNP_116618.1. NM_001179931.1.

Genome annotation databases

EnsemblFungiiBAA09204; BAA09204; BAA09204.
YFL034C-B; YFL034C-B; YFL034C-B.
GeneIDi850508.
KEGGisce:YFL034C-B.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09204.1. Sequence problems.
BK006940 Genomic DNA. Translation: DAA12405.1.
PIRiS58648.
RefSeqiNP_116618.1. NM_001179931.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LQPX-ray4.50B46-287[»]
4LQQX-ray3.60B/E46-287[»]
4LQSX-ray3.30B46-287[»]
ProteinModelPortaliP43563.
SMRiP43563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31111. 34 interactors.
DIPiDIP-1449N.
IntActiP43563. 14 interactors.
MINTiMINT-391527.

PTM databases

iPTMnetiP43563.

Proteomic databases

MaxQBiP43563.
PRIDEiP43563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09204; BAA09204; BAA09204.
YFL034C-B; YFL034C-B; YFL034C-B.
GeneIDi850508.
KEGGisce:YFL034C-B.

Organism-specific databases

EuPathDBiFungiDB:YFL034C-B.
SGDiS000001859. MOB2.

Phylogenomic databases

HOGENOMiHOG000164685.
InParanoidiP43563.
OMAiSEPFVRT.
OrthoDBiEOG092C4XVR.

Enzyme and pathway databases

BioCyciYEAST:G3O-30427-MONOMER.

Miscellaneous databases

PROiP43563.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. MOB_kinase_act_fam.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SMARTiSM01388. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMOB2_YEAST
AccessioniPrimary (citable) accession number: P43563
Secondary accession number(s): D6VTJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.