Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Iron transport multicopper oxidase FET5

Gene

FET5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron transport multicopper oxidase, which is required for Fe2+ high affinity uptake. May be required to oxidize Fe2+ and release it from the transporter. Essential component of copper-dependent iron transport.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi79 – 791Copper 1; type 2By similarity
Metal bindingi81 – 811Copper 2; type 3By similarity
Metal bindingi128 – 1281Copper 2; type 3By similarity
Metal bindingi130 – 1301Copper 3; type 3By similarity
Metal bindingi418 – 4181Copper 4; type 1By similarity
Metal bindingi421 – 4211Copper 1; type 2By similarity
Metal bindingi423 – 4231Copper 3; type 3By similarity
Metal bindingi496 – 4961Copper 3; type 3By similarity
Metal bindingi497 – 4971Copper 4; type 1By similarity
Metal bindingi498 – 4981Copper 2; type 3By similarity
Metal bindingi502 – 5021Copper 4; type 1By similarity

GO - Molecular functioni

  • copper ion binding Source: GO_Central
  • ferroxidase activity Source: SGD

GO - Biological processi

  • iron assimilation by reduction and transport Source: GO_Central
  • iron ion transmembrane transport Source: GOC
  • iron ion transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Copper, Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YFL041W-MONOMER.

Protein family/group databases

TCDBi2.A.108.1.4. the iron/lead transporter (ilt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron transport multicopper oxidase FET5 (EC:1.-.-.-)
Gene namesi
Name:FET5
Ordered Locus Names:YFL041W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL041W.
SGDiS000001853. FET5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 573555ExtracellularSequence analysisAdd
BLAST
Transmembranei574 – 59421HelicalSequence analysisAdd
BLAST
Topological domaini595 – 62228CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 622604Iron transport multicopper oxidase FET5PRO_0000002960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence analysis
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence analysis
Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence analysis
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence analysis
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence analysis
Glycosylationi364 – 3641N-linked (GlcNAc...)Sequence analysis
Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP43561.
TopDownProteomicsiP43561.

PTM databases

iPTMnetiP43561.

Interactioni

Subunit structurei

Interacts with FTH1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FTH1P383108EBI-6891,EBI-20959

Protein-protein interaction databases

BioGridi31105. 55 interactions.
DIPiDIP-6774N.
IntActiP43561. 7 interactions.
MINTiMINT-639126.

Structurei

3D structure databases

ProteinModelPortaliP43561.
SMRiP43561. Positions 8-555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 146104Plastocyanin-like 1Add
BLAST
Domaini192 – 301110Plastocyanin-like 2Add
BLAST
Domaini392 – 514123Plastocyanin-like 3Add
BLAST

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000064508.
HOGENOMiHOG000159793.
InParanoidiP43561.
KOiK19791.
OrthoDBiEOG7VB2Q3.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFYSFVWSV LAASVALAKT HKLNYTASWV TANPDGLHEK RMIGFNGEWP
60 70 80 90 100
LPDIHVEKGD RVELYLTNGF QDNTATSLHF HGLFQNTSLG NQLQMDGPSM
110 120 130 140 150
VTQCPIVPGQ TYLYNFTVPE QVGTFWYHAH MGAQYGDGMR GAFIIHDPEE
160 170 180 190 200
PFEYDHERVI TLSDHYHENY KTVTKEFLSR YNPTGAEPIP QNILFNNTMN
210 220 230 240 250
VTLDFTPGET YLFRFLNVGL FVSQYIILED HEMSIVEVDG VYVKPNFTDS
260 270 280 290 300
IYLSAGQRMS VLIKAKDKMP TRNYAMMQIM DETMLDVVPP ELQLNQTIQM
310 320 330 340 350
RYGHSLPEAR ALNIEDCDLD RATNDFYLEP LIERDLLAHY DHQIVMDVRM
360 370 380 390 400
VNLGDGVKYA FFNNITYVTP KVPTLTTLLT SGKLASDPRI YGDNINAQLL
410 420 430 440 450
KHNDIIEVVL NNYDSGRHPF HLHGHNFQIV QKSPGFHVDE AYDESEQDEM
460 470 480 490 500
TVPYNESAPL QPFPERPMVR DTVVLEPSGH VVLRFRADNP GVWYFHCHVD
510 520 530 540 550
WHLQQGLASV FIEAPVLLQE REKLNENYLD ICKAADIPVV GNAAGHSNDW
560 570 580 590 600
FDLKGLPRQP EPLPKGFTTE GYLALIISTI IGVWGLYSIA QYGIGEVIPN
610 620
DEKVYHTLRE ILAENEIEVS RG
Length:622
Mass (Da):70,880
Last modified:November 1, 1995 - v1
Checksum:i961757A6C86B7FAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09199.1.
BK006940 Genomic DNA. Translation: DAA12399.1.
PIRiS56214.
RefSeqiNP_116612.1. NM_001179925.1.

Genome annotation databases

EnsemblFungiiBAA09199; BAA09199; BAA09199.
YFL041W; YFL041W; YFL041W.
GeneIDi850502.
KEGGisce:YFL041W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09199.1.
BK006940 Genomic DNA. Translation: DAA12399.1.
PIRiS56214.
RefSeqiNP_116612.1. NM_001179925.1.

3D structure databases

ProteinModelPortaliP43561.
SMRiP43561. Positions 8-555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31105. 55 interactions.
DIPiDIP-6774N.
IntActiP43561. 7 interactions.
MINTiMINT-639126.

Protein family/group databases

TCDBi2.A.108.1.4. the iron/lead transporter (ilt) family.

PTM databases

iPTMnetiP43561.

Proteomic databases

MaxQBiP43561.
TopDownProteomicsiP43561.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09199; BAA09199; BAA09199.
YFL041W; YFL041W; YFL041W.
GeneIDi850502.
KEGGisce:YFL041W.

Organism-specific databases

EuPathDBiFungiDB:YFL041W.
SGDiS000001853. FET5.

Phylogenomic databases

GeneTreeiENSGT00530000064508.
HOGENOMiHOG000159793.
InParanoidiP43561.
KOiK19791.
OrthoDBiEOG7VB2Q3.

Enzyme and pathway databases

BioCyciYEAST:YFL041W-MONOMER.

Miscellaneous databases

PROiP43561.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The yeast FET5 gene encodes a FET3-related multicopper oxidase implicated in iron transport."
    Spizzo T., Byersdorfer C., Duesterhoeft S., Eide D.
    Mol. Gen. Genet. 256:547-556(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "The iron transporter Fth1p forms a complex with the Fet5 iron oxidase and resides on the vacuolar membrane."
    Urbanowski J.L., Piper R.C.
    J. Biol. Chem. 274:38061-38070(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FTH1.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFET5_YEAST
AccessioniPrimary (citable) accession number: P43561
Secondary accession number(s): D6VTI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.