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Protein

Iron transport multicopper oxidase FET5

Gene

FET5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron transport multicopper oxidase, which is required for Fe2+ high affinity uptake. May be required to oxidize Fe2+ and release it from the transporter. Essential component of copper-dependent iron transport.

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.1 Publication

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79Copper 1; type 2By similarity1
Metal bindingi81Copper 2; type 3By similarity1
Metal bindingi128Copper 2; type 3By similarity1
Metal bindingi130Copper 3; type 3By similarity1
Metal bindingi418Copper 4; type 1By similarity1
Metal bindingi421Copper 1; type 2By similarity1
Metal bindingi423Copper 3; type 3By similarity1
Metal bindingi496Copper 3; type 3By similarity1
Metal bindingi497Copper 4; type 1By similarity1
Metal bindingi498Copper 2; type 3By similarity1
Metal bindingi502Copper 4; type 1By similarity1

GO - Molecular functioni

  • copper ion binding Source: InterPro
  • ferroxidase activity Source: SGD

GO - Biological processi

  • iron assimilation by reduction and transport Source: GO_Central
  • iron ion transmembrane transport Source: GOC
  • iron ion transport Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processIon transport, Iron transport, Transport
LigandCopper, Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YFL041W-MONOMER.

Protein family/group databases

CAZyiAA1. Auxiliary Activities 1.
TCDBi2.A.108.1.4. the iron/lead transporter (ilt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron transport multicopper oxidase FET5 (EC:1.-.-.-)
Gene namesi
Name:FET5
Ordered Locus Names:YFL041W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL041W.
SGDiS000001853. FET5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 573ExtracellularSequence analysisAdd BLAST555
Transmembranei574 – 594HelicalSequence analysisAdd BLAST21
Topological domaini595 – 622CytoplasmicSequence analysisAdd BLAST28

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000000296019 – 622Iron transport multicopper oxidase FET5Add BLAST604

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi115N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi196N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi200N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi246N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi295N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi364N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi455N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP43561.
PRIDEiP43561.
TopDownProteomicsiP43561.

PTM databases

iPTMnetiP43561.

Interactioni

Subunit structurei

Interacts with FTH1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FTH1P383107EBI-6891,EBI-20959

Protein-protein interaction databases

BioGridi31105. 72 interactors.
DIPiDIP-6774N.
IntActiP43561. 7 interactors.
MINTiMINT-639126.
STRINGi4932.YFL041W.

Structurei

3D structure databases

ProteinModelPortaliP43561.
SMRiP43561.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 146Plastocyanin-like 1Add BLAST104
Domaini192 – 301Plastocyanin-like 2Add BLAST110
Domaini392 – 514Plastocyanin-like 3Add BLAST123

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000064508.
HOGENOMiHOG000159793.
InParanoidiP43561.
KOiK19791.
OMAiHPHCNTL.
OrthoDBiEOG092C10OL.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiView protein in InterPro
IPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
PfamiView protein in Pfam
PF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiView protein in PROSITE
PS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFYSFVWSV LAASVALAKT HKLNYTASWV TANPDGLHEK RMIGFNGEWP
60 70 80 90 100
LPDIHVEKGD RVELYLTNGF QDNTATSLHF HGLFQNTSLG NQLQMDGPSM
110 120 130 140 150
VTQCPIVPGQ TYLYNFTVPE QVGTFWYHAH MGAQYGDGMR GAFIIHDPEE
160 170 180 190 200
PFEYDHERVI TLSDHYHENY KTVTKEFLSR YNPTGAEPIP QNILFNNTMN
210 220 230 240 250
VTLDFTPGET YLFRFLNVGL FVSQYIILED HEMSIVEVDG VYVKPNFTDS
260 270 280 290 300
IYLSAGQRMS VLIKAKDKMP TRNYAMMQIM DETMLDVVPP ELQLNQTIQM
310 320 330 340 350
RYGHSLPEAR ALNIEDCDLD RATNDFYLEP LIERDLLAHY DHQIVMDVRM
360 370 380 390 400
VNLGDGVKYA FFNNITYVTP KVPTLTTLLT SGKLASDPRI YGDNINAQLL
410 420 430 440 450
KHNDIIEVVL NNYDSGRHPF HLHGHNFQIV QKSPGFHVDE AYDESEQDEM
460 470 480 490 500
TVPYNESAPL QPFPERPMVR DTVVLEPSGH VVLRFRADNP GVWYFHCHVD
510 520 530 540 550
WHLQQGLASV FIEAPVLLQE REKLNENYLD ICKAADIPVV GNAAGHSNDW
560 570 580 590 600
FDLKGLPRQP EPLPKGFTTE GYLALIISTI IGVWGLYSIA QYGIGEVIPN
610 620
DEKVYHTLRE ILAENEIEVS RG
Length:622
Mass (Da):70,880
Last modified:November 1, 1995 - v1
Checksum:i961757A6C86B7FAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09199.1.
BK006940 Genomic DNA. Translation: DAA12399.1.
PIRiS56214.
RefSeqiNP_116612.1. NM_001179925.1.

Genome annotation databases

EnsemblFungiiBAA09199; BAA09199; BAA09199.
YFL041W; YFL041W; YFL041W.
GeneIDi850502.
KEGGisce:YFL041W.

Similar proteinsi

Entry informationi

Entry nameiFET5_YEAST
AccessioniPrimary (citable) accession number: P43561
Secondary accession number(s): D6VTI9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 25, 2017
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names