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P43561 (FET5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Iron transport multicopper oxidase FET5
EC=1.-.-.-
Gene names
Name:FET5
Ordered Locus Names:YFL041W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Iron transport multicopper oxidase, which is required for Fe2+ high affinity uptake. May be required to oxidize Fe2+ and release it from the transporter. Essential component of copper-dependent iron transport.

Cofactor

Binds 4 copper ions per monomer By similarity.

Subunit structure

Interacts with FTH1. Ref.4

Subcellular location

Cell membrane; Single-pass membrane protein Potential.

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the multicopper oxidase family.

Contains 3 plastocyanin-like domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FTH1P383107EBI-6891,EBI-20959

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 622604Iron transport multicopper oxidase FET5
PRO_0000002960

Regions

Topological domain19 – 573555Extracellular Potential
Transmembrane574 – 59421Helical; Potential
Topological domain595 – 62228Cytoplasmic Potential
Domain43 – 146104Plastocyanin-like 1
Domain192 – 301110Plastocyanin-like 2
Domain392 – 514123Plastocyanin-like 3

Sites

Metal binding791Copper 1; type 2 By similarity
Metal binding811Copper 2; type 3 By similarity
Metal binding1281Copper 2; type 3 By similarity
Metal binding1301Copper 3; type 3 By similarity
Metal binding4181Copper 4; type 1 By similarity
Metal binding4211Copper 1; type 2 By similarity
Metal binding4231Copper 3; type 3 By similarity
Metal binding4961Copper 3; type 3 By similarity
Metal binding4971Copper 4; type 1 By similarity
Metal binding4981Copper 2; type 3 By similarity
Metal binding5021Copper 4; type 1 By similarity

Amino acid modifications

Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation1151N-linked (GlcNAc...) Potential
Glycosylation1961N-linked (GlcNAc...) Potential
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation3641N-linked (GlcNAc...) Potential
Glycosylation4551N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P43561 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 961757A6C86B7FAF

FASTA62270,880
        10         20         30         40         50         60 
MLFYSFVWSV LAASVALAKT HKLNYTASWV TANPDGLHEK RMIGFNGEWP LPDIHVEKGD 

        70         80         90        100        110        120 
RVELYLTNGF QDNTATSLHF HGLFQNTSLG NQLQMDGPSM VTQCPIVPGQ TYLYNFTVPE 

       130        140        150        160        170        180 
QVGTFWYHAH MGAQYGDGMR GAFIIHDPEE PFEYDHERVI TLSDHYHENY KTVTKEFLSR 

       190        200        210        220        230        240 
YNPTGAEPIP QNILFNNTMN VTLDFTPGET YLFRFLNVGL FVSQYIILED HEMSIVEVDG 

       250        260        270        280        290        300 
VYVKPNFTDS IYLSAGQRMS VLIKAKDKMP TRNYAMMQIM DETMLDVVPP ELQLNQTIQM 

       310        320        330        340        350        360 
RYGHSLPEAR ALNIEDCDLD RATNDFYLEP LIERDLLAHY DHQIVMDVRM VNLGDGVKYA 

       370        380        390        400        410        420 
FFNNITYVTP KVPTLTTLLT SGKLASDPRI YGDNINAQLL KHNDIIEVVL NNYDSGRHPF 

       430        440        450        460        470        480 
HLHGHNFQIV QKSPGFHVDE AYDESEQDEM TVPYNESAPL QPFPERPMVR DTVVLEPSGH 

       490        500        510        520        530        540 
VVLRFRADNP GVWYFHCHVD WHLQQGLASV FIEAPVLLQE REKLNENYLD ICKAADIPVV 

       550        560        570        580        590        600 
GNAAGHSNDW FDLKGLPRQP EPLPKGFTTE GYLALIISTI IGVWGLYSIA QYGIGEVIPN 

       610        620 
DEKVYHTLRE ILAENEIEVS RG

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The yeast FET5 gene encodes a FET3-related multicopper oxidase implicated in iron transport."
Spizzo T., Byersdorfer C., Duesterhoeft S., Eide D.
Mol. Gen. Genet. 256:547-556(1997) [PubMed: 9413439] [Abstract]
Cited for: CHARACTERIZATION.
[4]"The iron transporter Fth1p forms a complex with the Fet5 iron oxidase and resides on the vacuolar membrane."
Urbanowski J.L., Piper R.C.
J. Biol. Chem. 274:38061-38070(1999) [PubMed: 10608875] [Abstract]
Cited for: INTERACTION WITH FTH1.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D50617 Genomic DNA. Translation: BAA09199.1.
BK006940 Genomic DNA. Translation: DAA12399.1.
PIRS56214.
RefSeqNP_116612.1. NM_001179925.1.

3D structure databases

ProteinModelPortalP43561.
SMRP43561. Positions 22-538.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6774N.
IntActP43561. 7 interactions.
MINTMINT-639126.
STRINGP43561.

Protein family/group databases

TCDB9.A.10.1.4. iron/lead transporter (ILT) superfamily.

Proteomic databases

PeptideAtlasP43561.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFL041W; YFL041W; YFL041W.
GeneID850502.
KEGGsce:YFL041W.
NMPDRfig|4932.3.peg.2237.

Organism-specific databases

CYGDYFL041w.
SGDS000001853. FET5.

Phylogenomic databases

eggNOGfuNOG05204.
GeneTreeEFGT00050000000233.
HOGENOMHBG324004.
OrthoDBEOG4T4H3S.

Gene expression databases

ArrayExpressP43561.
GenevestigatorP43561.
GermOnlineYFL041W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 3 hits.
KOK00540.
PfamPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMSSF49503. Cupredoxin. 3 hits.
PROSITEPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966200.

Entry information

Entry nameFET5_YEAST
AccessionPrimary (citable) accession number: P43561
Secondary accession number(s): D6VTI9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: December 14, 2011
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

SIMILARITY comments

Index of protein domains and families

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