P43560 (YFE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YFL042C

Gene names

Ordered Locus Names:

YFL042C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the YSP2 family. Contains 1 GRAM domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	Uncharacterized protein YFL042C		PRO_0000202673
Regions
	Transmembrane	634 – 654	21	Helical; Potential
	Domain	198 – 265	68	GRAM
	Compositional bias	81 – 140	60	Ser-rich
Amino acid modifications
	Modified residue	43	1	Phosphoserine Ref.5
	Modified residue	110	1	Phosphothreonine Ref.4 Ref.5
	Modified residue	112	1	Phosphothreonine Ref.5
	Modified residue	113	1	Phosphoserine Ref.4 Ref.5
	Modified residue	140	1	Phosphoserine Ref.5
	Modified residue	143	1	Phosphothreonine Ref.4
	Modified residue	149	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P43560 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 161F12303E1102C4	FASTA	674	76,347
	10 20 30 40 50 60 MSDVDNWEPV SDNEDSTDSV KQLGPPFEHA SNNDNAGDTE AESLQEVPLN TETNDVRKNL 70 80 90 100 110 120 VVITNQSAAD EHPTEIKHDQ SRTSSTSSFF SGMISSFKSN VPSPVSRSTT PTSPVSQPSI 130 140 150 160 170 180 ISHRREPSMG SKRRSSRRIS NATIAEIGSP LQQVEKPDEV KTRLTPSQMK EDNYDHRRFV 190 200 210 220 230 240 EERYMDTPYH YASEQRNKDF HETFKSVPKD DRLLDDFNCG LNRELLYQGK LYITETHLCF 250 260 270 280 290 300 NSNVLGWIAK VLIAFEDVTF MEKTSAAGLF PSAISIETKM GKTLFNGFIS RDAAFGLMKE 310 320 330 340 350 360 VWSRTLLQKD MASENINTKA EKSGNGKEID DAINSIDEEN NDKDANDNDT NENDDENIST 370 380 390 400 410 420 NETTPNSTSS SPDKEKEKAY KLRADSSYQY DGPIYHHSTS FPAEPMANNE FVLKELPFDC 430 440 450 460 470 480 APGILFEIMF NSEQNEFLLD FLRGQEGSQI TTIPNFTSID GSSMTLKREY SYEKALHFPA 490 500 510 520 530 540 GPKSTTCYVA EVIKRKDPDT YYEVISSIRT PNVPSGGSFS TKTRYLIRWN DEITCLLRVS 550 560 570 580 590 600 FWVEWTGSSW IKGMVENGCK NGQLEAAQLM ERILSKFIKN NVEECQITIS KEEEEQDDKE 610 620 630 640 650 660 VKNKLKEVDL EQPREAVVTA PAIAEQQGLK VTMETWLFLY LIVVVLLLFN LFYIRSIAVS 670 LHQLVKLQLV ELKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Murakami Y. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-110; SER-113; THR-143 AND SER-149, MASS SPECTROMETRY. Strain: ADR376.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; THR-110; THR-112; SER-113 AND SER-140, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA24424.1. BK006940 Genomic DNA. Translation: DAA12397.1.
PIR	S78570.
RefSeq	NP_116611.1. NM_001179924.1.
3D structure databases
ProteinModelPortal	P43560.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1182N.
IntAct	P43560. 5 interactions.
MINT	MINT-393348.
Proteomic databases
PaxDb	P43560.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL042C; YFL042C; YFL042C.
GeneID	850501.
KEGG	sce:YFL042C.
Organism-specific databases
CYGD	YFL042c.
SGD	S000001852. YFL042C.
Phylogenomic databases
eggNOG	NOG304071.
GeneTree	ENSGT00580000081380.
OMA	MASENIN.
OrthoDB	EOG42FWSS.
Gene expression databases
Genevestigator	P43560.
GermOnline	YFL042C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004182. GRAM. [Graphical view]
Pfam	PF02893. GRAM. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966197.

Entry information

Entry name

YFE2_YEAST

Accession

Primary (citable) accession number: P43560
Secondary accession number(s): D6VTI7, P43559

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 15, 1999
Last modified:	April 3, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program