Ubiquitin thioesterase OTU1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P43558 (OTU1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase OTU1
EC=3.4.19.12

Alternative name(s):
OTU domain-containing protein 1

Gene names

Name:	OTU1
Ordered Locus Names:	YFL044C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Participates in the regulation of the ubiquin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May be indireclty involved in PIS1 gene expression. Ref.5 Ref.6
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with CDC48 and DOA1/UFD3. Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.3.
Domain	The OTU domain mediates the hydrolase activity and the interaction with CDC48. Ref.6
Miscellaneous	Present with 2770 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from direct assay Ref.6. Source: SGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.3. Source: SGD nucleus Inferred from direct assay Ref.3. Source: SGD
Molecular_function	ubiquitin-specific protease activity Inferred from direct assay Ref.6. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 301

301

Ubiquitin thioesterase OTU1

PRO_0000202672

Regions

Domain

109 – 229

121

OTU

Zinc finger

270 – 294

C2H2-type

Sites

Active site

117

By similarity

Active site

120

Nucleophile Probable

Active site

294

By similarity

Experimental info

Mutagenesis

120

C → S: Loss of function. Ref.6

Secondary structure

301

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P43558 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 32ABA3CFEA4BB5EB
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FASTA

301

33,510

        10         20         30         40         50         60 
MKLKVTGAGI NQVVTLKQDA TLNDLIEHIN VDVKTMRFGY PPQRINLQGE DASLGQTQLD 

        70         80         90        100        110        120 
ELGINSGEKI TIESSDSNES FSLPPPQPKP KRVLKSTEMS IGGSGENVLS VHPVLDDNSC 

       130        140        150        160        170        180 
LFHAIAYGIF KQDSVRDLRE MVSKEVLNNP VKFNDAILDK PNKDYAQWIL KMESWGGAIE 

       190        200        210        220        230        240 
IGIISDALAV AIYVVDIDAV KIEKFNEDKF DNYILILFNG IHYDSLTMNE FKTVFNKNQP 

       250        260        270        280        290        300 
ESDDVLTAAL QLASNLKQTG YSFNTHKAQI KCNTCQMTFV GEREVARHAE STGHVDFGQN 


R

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Genomic analysis of PIS1 gene expression." Gardocki M.E., Bakewell M., Kamath D., Robinson K., Borovicka K., Lopes J.M. Eukaryot. Cell 4:604-614(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone." Rumpf S., Jentsch S. Mol. Cell 21:261-269(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DOMAIN OTU, INTERACTION WITH CDC48 AND DOA1, MUTAGENESIS OF CYS-120.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D50617 Genomic DNA. Translation: BAA09197.1.
BK006940 Genomic DNA. Translation: DAA12396.1.

PIR

S56211.

RefSeq

NP_116610.1. NM_001179923.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BY4	X-ray	1.55	A	91-301	[»]
3C0R	X-ray	2.31	A/C	91-301	[»]

ProteinModelPortal

P43558.

SMR

P43558. Positions 92-266.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4672N.

IntAct

P43558. 10 interactions.

MINT

MINT-561059.

STRING

4932.YFL044C.

Protein family/group databases

MEROPS

C85.006.

Proteomic databases

PaxDb

P43558.

PeptideAtlas

P43558.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFL044C; YFL044C; YFL044C.

GeneID

850500.

KEGG

sce:YFL044C.

Organism-specific databases

CYGD

YFL044c.

SGD

S000001850. OTU1.

Phylogenomic databases

eggNOG

COG5539.

GeneTree

ENSGT00390000009989.

HOGENOM

HOG000193461.

K13719.

OMA

SCLFHAI.

OrthoDB

EOG42NN8R.

Gene expression databases

Genevestigator

P43558.

GermOnline

YFL044C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR003323. OTU.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]

Pfam

PF02338. OTU. 1 hit.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 1 hit.
[Graphical view]

PROSITE

PS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P43558.

NextBio

966194.

Entry information

Entry name

OTU1_YEAST

Accession

Primary (citable) accession number: P43558
Secondary accession number(s): D6VTI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents