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Protein

Ubiquitin thioesterase OTU1

Gene

OTU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Participates in the regulation of the ubiquin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May be indireclty involved in PIS1 gene expression.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei117By similarity1
Active sitei120NucleophileCurated1
Binding sitei221Substrate; via carbonyl oxygenBy similarity1
Active sitei222By similarity1
Active sitei294By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri270 – 294C2H2-typeAdd BLAST25

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • thiol-dependent ubiquitin-specific protease activity Source: SGD

GO - Biological processi

  • endoplasmic reticulum unfolded protein response Source: GO_Central
  • ER-associated ubiquitin-dependent protein catabolic process Source: GO_Central
  • protein deubiquitination Source: SGD
  • regulation of transcription, DNA-templated Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30420-MONOMER.
ReactomeiR-SCE-5689896. Ovarian tumor domain proteases.

Protein family/group databases

MEROPSiC85.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase OTU1 (EC:3.4.19.12By similarity)
Alternative name(s):
OTU domain-containing protein 1
Gene namesi
Name:OTU1
Ordered Locus Names:YFL044C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL044C.
SGDiS000001850. OTU1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120C → S: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026721 – 301Ubiquitin thioesterase OTU1Add BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki160Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP43558.
PRIDEiP43558.

PTM databases

iPTMnetiP43558.

Interactioni

Subunit structurei

Interacts with CDC48 and DOA1/UFD3.1 Publication

Protein-protein interaction databases

BioGridi31103. 77 interactors.
DIPiDIP-4672N.
IntActiP43558. 13 interactors.
MINTiMINT-561059.

Structurei

Secondary structure

1301
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 7Combined sources7
Beta strandi10 – 16Combined sources7
Helixi22 – 28Combined sources7
Beta strandi34 – 38Combined sources5
Turni39 – 42Combined sources4
Beta strandi43 – 46Combined sources4
Helixi54 – 56Combined sources3
Helixi60 – 62Combined sources3
Beta strandi68 – 73Combined sources6
Beta strandi98 – 101Combined sources4
Turni102 – 104Combined sources3
Beta strandi107 – 112Combined sources6
Helixi120 – 130Combined sources11
Helixi136 – 148Combined sources13
Turni150 – 153Combined sources4
Helixi155 – 158Combined sources4
Helixi162 – 168Combined sources7
Helixi178 – 188Combined sources11
Beta strandi191 – 196Combined sources6
Turni197 – 200Combined sources4
Beta strandi201 – 206Combined sources6
Turni207 – 209Combined sources3
Beta strandi211 – 218Combined sources8
Beta strandi223 – 228Combined sources6
Turni229 – 231Combined sources3
Helixi242 – 258Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BY4X-ray1.55A91-301[»]
3C0RX-ray2.31A/C91-301[»]
4KDIX-ray1.86C/D1-73[»]
4KDLX-ray1.81B1-73[»]
ProteinModelPortaliP43558.
SMRiP43558.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43558.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 229OTUPROSITE-ProRule annotationAdd BLAST121

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 80UBX-likeBy similarityAdd BLAST77
Regioni114 – 120Cys-loopBy similarity7
Regioni169 – 179Variable-loopBy similarityAdd BLAST11
Regioni218 – 222His-loopBy similarity5
Regioni243 – 248S2 siteBy similarity6

Domaini

The OTU domain mediates the hydrolase activity and the interaction with CDC48.1 Publication

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri270 – 294C2H2-typeAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000009989.
HOGENOMiHOG000193461.
InParanoidiP43558.
KOiK13719.
OMAiRFGYPPQ.
OrthoDBiEOG092C3UUZ.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR007087. Znf_C2H2.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKVTGAGI NQVVTLKQDA TLNDLIEHIN VDVKTMRFGY PPQRINLQGE
60 70 80 90 100
DASLGQTQLD ELGINSGEKI TIESSDSNES FSLPPPQPKP KRVLKSTEMS
110 120 130 140 150
IGGSGENVLS VHPVLDDNSC LFHAIAYGIF KQDSVRDLRE MVSKEVLNNP
160 170 180 190 200
VKFNDAILDK PNKDYAQWIL KMESWGGAIE IGIISDALAV AIYVVDIDAV
210 220 230 240 250
KIEKFNEDKF DNYILILFNG IHYDSLTMNE FKTVFNKNQP ESDDVLTAAL
260 270 280 290 300
QLASNLKQTG YSFNTHKAQI KCNTCQMTFV GEREVARHAE STGHVDFGQN

R
Length:301
Mass (Da):33,510
Last modified:November 1, 1995 - v1
Checksum:i32ABA3CFEA4BB5EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09197.1.
BK006940 Genomic DNA. Translation: DAA12396.1.
PIRiS56211.
RefSeqiNP_116610.1. NM_001179923.1.

Genome annotation databases

EnsemblFungiiBAA09197; BAA09197; BAA09197.
YFL044C; YFL044C; YFL044C.
GeneIDi850500.
KEGGisce:YFL044C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09197.1.
BK006940 Genomic DNA. Translation: DAA12396.1.
PIRiS56211.
RefSeqiNP_116610.1. NM_001179923.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BY4X-ray1.55A91-301[»]
3C0RX-ray2.31A/C91-301[»]
4KDIX-ray1.86C/D1-73[»]
4KDLX-ray1.81B1-73[»]
ProteinModelPortaliP43558.
SMRiP43558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31103. 77 interactors.
DIPiDIP-4672N.
IntActiP43558. 13 interactors.
MINTiMINT-561059.

Protein family/group databases

MEROPSiC85.006.

PTM databases

iPTMnetiP43558.

Proteomic databases

MaxQBiP43558.
PRIDEiP43558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09197; BAA09197; BAA09197.
YFL044C; YFL044C; YFL044C.
GeneIDi850500.
KEGGisce:YFL044C.

Organism-specific databases

EuPathDBiFungiDB:YFL044C.
SGDiS000001850. OTU1.

Phylogenomic databases

GeneTreeiENSGT00390000009989.
HOGENOMiHOG000193461.
InParanoidiP43558.
KOiK13719.
OMAiRFGYPPQ.
OrthoDBiEOG092C3UUZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30420-MONOMER.
ReactomeiR-SCE-5689896. Ovarian tumor domain proteases.

Miscellaneous databases

EvolutionaryTraceiP43558.
PROiP43558.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR007087. Znf_C2H2.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTU1_YEAST
AccessioniPrimary (citable) accession number: P43558
Secondary accession number(s): D6VTI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.