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P43558 (OTU1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin thioesterase OTU1

EC=3.4.19.12
Alternative name(s):
OTU domain-containing protein 1
Gene names
Name:OTU1
Ordered Locus Names:YFL044C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Participates in the regulation of the ubiquin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May be indireclty involved in PIS1 gene expression. Ref.5 Ref.6

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with CDC48 and DOA1/UFD3. Ref.6

Subcellular location

Cytoplasm. Nucleus Ref.3.

Domain

The OTU domain mediates the hydrolase activity and the interaction with CDC48. Ref.6

Miscellaneous

Present with 2770 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 C2H2-type zinc finger.

Contains 1 OTU domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301Ubiquitin thioesterase OTU1
PRO_0000202672

Regions

Domain109 – 229121OTU
Zinc finger270 – 29425C2H2-type

Sites

Active site1171 By similarity
Active site1201Nucleophile Probable
Active site2941 By similarity

Experimental info

Mutagenesis1201C → S: Loss of function. Ref.6

Secondary structure

.............................. 301
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43558 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 32ABA3CFEA4BB5EB

FASTA30133,510
        10         20         30         40         50         60 
MKLKVTGAGI NQVVTLKQDA TLNDLIEHIN VDVKTMRFGY PPQRINLQGE DASLGQTQLD 

        70         80         90        100        110        120 
ELGINSGEKI TIESSDSNES FSLPPPQPKP KRVLKSTEMS IGGSGENVLS VHPVLDDNSC 

       130        140        150        160        170        180 
LFHAIAYGIF KQDSVRDLRE MVSKEVLNNP VKFNDAILDK PNKDYAQWIL KMESWGGAIE 

       190        200        210        220        230        240 
IGIISDALAV AIYVVDIDAV KIEKFNEDKF DNYILILFNG IHYDSLTMNE FKTVFNKNQP 

       250        260        270        280        290        300 
ESDDVLTAAL QLASNLKQTG YSFNTHKAQI KCNTCQMTFV GEREVARHAE STGHVDFGQN 


R 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Genomic analysis of PIS1 gene expression."
Gardocki M.E., Bakewell M., Kamath D., Robinson K., Borovicka K., Lopes J.M.
Eukaryot. Cell 4:604-614(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone."
Rumpf S., Jentsch S.
Mol. Cell 21:261-269(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DOMAIN OTU, INTERACTION WITH CDC48 AND DOA1, MUTAGENESIS OF CYS-120.
[7]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50617 Genomic DNA. Translation: BAA09197.1.
BK006940 Genomic DNA. Translation: DAA12396.1.
PIRS56211.
RefSeqNP_116610.1. NM_001179923.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3BY4X-ray1.55A91-301[»]
3C0RX-ray2.31A/C91-301[»]
ProteinModelPortalP43558.
SMRP43558. Positions 92-266.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31103. 74 interactions.
DIPDIP-4672N.
IntActP43558. 11 interactions.
MINTMINT-561059.
STRING4932.YFL044C.

Protein family/group databases

MEROPSC85.006.

Proteomic databases

PaxDbP43558.
PeptideAtlasP43558.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFL044C; YFL044C; YFL044C.
GeneID850500.
KEGGsce:YFL044C.

Organism-specific databases

CYGDYFL044c.
SGDS000001850. OTU1.

Phylogenomic databases

eggNOGCOG5539.
GeneTreeENSGT00390000009989.
HOGENOMHOG000193461.
KOK13719.
OMASCLFHAI.
OrthoDBEOG790GCB.

Enzyme and pathway databases

BioCycYEAST:G3O-30420-MONOMER.

Gene expression databases

GenevestigatorP43558.

Family and domain databases

InterProIPR003323. OTU.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamPF02338. OTU. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP43558.
NextBio966194.
PROP43558.

Entry information

Entry nameOTU1_YEAST
AccessionPrimary (citable) accession number: P43558
Secondary accession number(s): D6VTI6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references