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P43558

- OTU1_YEAST

UniProt

P43558 - OTU1_YEAST

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Protein

Ubiquitin thioesterase OTU1

Gene

OTU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Participates in the regulation of the ubiquin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May be indireclty involved in PIS1 gene expression.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei117 – 1171By similarity
Active sitei120 – 1201NucleophileCurated
Active sitei294 – 2941By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri270 – 29425C2H2-typeAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: SGD

GO - Biological processi

  1. protein deubiquitination Source: SGD
  2. regulation of transcription, DNA-templated Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30420-MONOMER.

Protein family/group databases

MEROPSiC85.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase OTU1 (EC:3.4.19.12)
Alternative name(s):
OTU domain-containing protein 1
Gene namesi
Name:OTU1
Ordered Locus Names:YFL044C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL044c.
SGDiS000001850. OTU1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi120 – 1201C → S: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Ubiquitin thioesterase OTU1PRO_0000202672Add
BLAST

Proteomic databases

MaxQBiP43558.
PaxDbiP43558.
PeptideAtlasiP43558.

Expressioni

Gene expression databases

GenevestigatoriP43558.

Interactioni

Subunit structurei

Interacts with CDC48 and DOA1/UFD3.1 Publication

Protein-protein interaction databases

BioGridi31103. 75 interactions.
DIPiDIP-4672N.
IntActiP43558. 11 interactions.
MINTiMINT-561059.
STRINGi4932.YFL044C.

Structurei

Secondary structure

301
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 77Combined sources
Beta strandi10 – 167Combined sources
Helixi22 – 287Combined sources
Beta strandi34 – 385Combined sources
Turni39 – 424Combined sources
Beta strandi43 – 464Combined sources
Helixi54 – 563Combined sources
Helixi60 – 623Combined sources
Beta strandi68 – 736Combined sources
Beta strandi98 – 1014Combined sources
Turni102 – 1043Combined sources
Beta strandi107 – 1126Combined sources
Helixi120 – 13011Combined sources
Helixi136 – 14813Combined sources
Turni150 – 1534Combined sources
Helixi155 – 1584Combined sources
Helixi162 – 1687Combined sources
Helixi178 – 18811Combined sources
Beta strandi191 – 1966Combined sources
Turni197 – 2004Combined sources
Beta strandi201 – 2066Combined sources
Turni207 – 2093Combined sources
Beta strandi211 – 2188Combined sources
Beta strandi223 – 2286Combined sources
Turni229 – 2313Combined sources
Helixi242 – 25817Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BY4X-ray1.55A91-301[»]
3C0RX-ray2.31A/C91-301[»]
4KDIX-ray1.86C/D1-73[»]
4KDLX-ray1.81B1-73[»]
ProteinModelPortaliP43558.
SMRiP43558. Positions 1-73, 92-266.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43558.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 229121OTUPROSITE-ProRule annotationAdd
BLAST

Domaini

The OTU domain mediates the hydrolase activity and the interaction with CDC48.1 Publication

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri270 – 29425C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5539.
GeneTreeiENSGT00390000009989.
HOGENOMiHOG000193461.
InParanoidiP43558.
KOiK13719.
OMAiSCLFHAI.
OrthoDBiEOG790GCB.

Family and domain databases

InterProiIPR003323. OTU.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43558-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLKVTGAGI NQVVTLKQDA TLNDLIEHIN VDVKTMRFGY PPQRINLQGE
60 70 80 90 100
DASLGQTQLD ELGINSGEKI TIESSDSNES FSLPPPQPKP KRVLKSTEMS
110 120 130 140 150
IGGSGENVLS VHPVLDDNSC LFHAIAYGIF KQDSVRDLRE MVSKEVLNNP
160 170 180 190 200
VKFNDAILDK PNKDYAQWIL KMESWGGAIE IGIISDALAV AIYVVDIDAV
210 220 230 240 250
KIEKFNEDKF DNYILILFNG IHYDSLTMNE FKTVFNKNQP ESDDVLTAAL
260 270 280 290 300
QLASNLKQTG YSFNTHKAQI KCNTCQMTFV GEREVARHAE STGHVDFGQN

R
Length:301
Mass (Da):33,510
Last modified:November 1, 1995 - v1
Checksum:i32ABA3CFEA4BB5EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09197.1.
BK006940 Genomic DNA. Translation: DAA12396.1.
PIRiS56211.
RefSeqiNP_116610.1. NM_001179923.1.

Genome annotation databases

EnsemblFungiiYFL044C; YFL044C; YFL044C.
GeneIDi850500.
KEGGisce:YFL044C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09197.1 .
BK006940 Genomic DNA. Translation: DAA12396.1 .
PIRi S56211.
RefSeqi NP_116610.1. NM_001179923.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3BY4 X-ray 1.55 A 91-301 [» ]
3C0R X-ray 2.31 A/C 91-301 [» ]
4KDI X-ray 1.86 C/D 1-73 [» ]
4KDL X-ray 1.81 B 1-73 [» ]
ProteinModelPortali P43558.
SMRi P43558. Positions 1-73, 92-266.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31103. 75 interactions.
DIPi DIP-4672N.
IntActi P43558. 11 interactions.
MINTi MINT-561059.
STRINGi 4932.YFL044C.

Protein family/group databases

MEROPSi C85.006.

Proteomic databases

MaxQBi P43558.
PaxDbi P43558.
PeptideAtlasi P43558.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL044C ; YFL044C ; YFL044C .
GeneIDi 850500.
KEGGi sce:YFL044C.

Organism-specific databases

CYGDi YFL044c.
SGDi S000001850. OTU1.

Phylogenomic databases

eggNOGi COG5539.
GeneTreei ENSGT00390000009989.
HOGENOMi HOG000193461.
InParanoidi P43558.
KOi K13719.
OMAi SCLFHAI.
OrthoDBi EOG790GCB.

Enzyme and pathway databases

BioCyci YEAST:G3O-30420-MONOMER.

Miscellaneous databases

EvolutionaryTracei P43558.
NextBioi 966194.
PROi P43558.

Gene expression databases

Genevestigatori P43558.

Family and domain databases

InterProi IPR003323. OTU.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: FUNCTION.
  6. "Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone."
    Rumpf S., Jentsch S.
    Mol. Cell 21:261-269(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN OTU, INTERACTION WITH CDC48 AND DOA1, MUTAGENESIS OF CYS-120.
  7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOTU1_YEAST
AccessioniPrimary (citable) accession number: P43558
Secondary accession number(s): D6VTI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3