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P43558

- OTU1_YEAST

UniProt

P43558 - OTU1_YEAST

Protein

Ubiquitin thioesterase OTU1

Gene

OTU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Participates in the regulation of the ubiquin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May be indireclty involved in PIS1 gene expression.2 Publications

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei117 – 1171By similarity
    Active sitei120 – 1201NucleophileCurated
    Active sitei294 – 2941By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri270 – 29425C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ubiquitin-specific protease activity Source: SGD

    GO - Biological processi

    1. protein deubiquitination Source: SGD
    2. regulation of transcription, DNA-templated Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30420-MONOMER.

    Protein family/group databases

    MEROPSiC85.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase OTU1 (EC:3.4.19.12)
    Alternative name(s):
    OTU domain-containing protein 1
    Gene namesi
    Name:OTU1
    Ordered Locus Names:YFL044C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL044c.
    SGDiS000001850. OTU1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi120 – 1201C → S: Loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 301301Ubiquitin thioesterase OTU1PRO_0000202672Add
    BLAST

    Proteomic databases

    MaxQBiP43558.
    PaxDbiP43558.
    PeptideAtlasiP43558.

    Expressioni

    Gene expression databases

    GenevestigatoriP43558.

    Interactioni

    Subunit structurei

    Interacts with CDC48 and DOA1/UFD3.1 Publication

    Protein-protein interaction databases

    BioGridi31103. 75 interactions.
    DIPiDIP-4672N.
    IntActiP43558. 11 interactions.
    MINTiMINT-561059.
    STRINGi4932.YFL044C.

    Structurei

    Secondary structure

    301
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi1 – 77
    Beta strandi10 – 167
    Helixi22 – 287
    Beta strandi34 – 385
    Turni39 – 424
    Beta strandi43 – 464
    Helixi54 – 563
    Helixi60 – 623
    Beta strandi68 – 736
    Beta strandi98 – 1014
    Turni102 – 1043
    Beta strandi107 – 1126
    Helixi120 – 13011
    Helixi136 – 14813
    Turni150 – 1534
    Helixi155 – 1584
    Helixi162 – 1687
    Helixi178 – 18811
    Beta strandi191 – 1966
    Turni197 – 2004
    Beta strandi201 – 2066
    Turni207 – 2093
    Beta strandi211 – 2188
    Beta strandi223 – 2286
    Turni229 – 2313
    Helixi242 – 25817

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3BY4X-ray1.55A91-301[»]
    3C0RX-ray2.31A/C91-301[»]
    4KDIX-ray1.86C/D1-73[»]
    4KDLX-ray1.81B1-73[»]
    ProteinModelPortaliP43558.
    SMRiP43558. Positions 1-73, 92-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43558.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini109 – 229121OTUPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The OTU domain mediates the hydrolase activity and the interaction with CDC48.1 Publication

    Sequence similaritiesi

    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri270 – 29425C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5539.
    GeneTreeiENSGT00390000009989.
    HOGENOMiHOG000193461.
    KOiK13719.
    OMAiSCLFHAI.
    OrthoDBiEOG790GCB.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43558-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLKVTGAGI NQVVTLKQDA TLNDLIEHIN VDVKTMRFGY PPQRINLQGE    50
    DASLGQTQLD ELGINSGEKI TIESSDSNES FSLPPPQPKP KRVLKSTEMS 100
    IGGSGENVLS VHPVLDDNSC LFHAIAYGIF KQDSVRDLRE MVSKEVLNNP 150
    VKFNDAILDK PNKDYAQWIL KMESWGGAIE IGIISDALAV AIYVVDIDAV 200
    KIEKFNEDKF DNYILILFNG IHYDSLTMNE FKTVFNKNQP ESDDVLTAAL 250
    QLASNLKQTG YSFNTHKAQI KCNTCQMTFV GEREVARHAE STGHVDFGQN 300
    R 301
    Length:301
    Mass (Da):33,510
    Last modified:November 1, 1995 - v1
    Checksum:i32ABA3CFEA4BB5EB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09197.1.
    BK006940 Genomic DNA. Translation: DAA12396.1.
    PIRiS56211.
    RefSeqiNP_116610.1. NM_001179923.1.

    Genome annotation databases

    EnsemblFungiiYFL044C; YFL044C; YFL044C.
    GeneIDi850500.
    KEGGisce:YFL044C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50617 Genomic DNA. Translation: BAA09197.1 .
    BK006940 Genomic DNA. Translation: DAA12396.1 .
    PIRi S56211.
    RefSeqi NP_116610.1. NM_001179923.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3BY4 X-ray 1.55 A 91-301 [» ]
    3C0R X-ray 2.31 A/C 91-301 [» ]
    4KDI X-ray 1.86 C/D 1-73 [» ]
    4KDL X-ray 1.81 B 1-73 [» ]
    ProteinModelPortali P43558.
    SMRi P43558. Positions 1-73, 92-266.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31103. 75 interactions.
    DIPi DIP-4672N.
    IntActi P43558. 11 interactions.
    MINTi MINT-561059.
    STRINGi 4932.YFL044C.

    Protein family/group databases

    MEROPSi C85.006.

    Proteomic databases

    MaxQBi P43558.
    PaxDbi P43558.
    PeptideAtlasi P43558.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL044C ; YFL044C ; YFL044C .
    GeneIDi 850500.
    KEGGi sce:YFL044C.

    Organism-specific databases

    CYGDi YFL044c.
    SGDi S000001850. OTU1.

    Phylogenomic databases

    eggNOGi COG5539.
    GeneTreei ENSGT00390000009989.
    HOGENOMi HOG000193461.
    KOi K13719.
    OMAi SCLFHAI.
    OrthoDBi EOG790GCB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30420-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P43558.
    NextBioi 966194.
    PROi P43558.

    Gene expression databases

    Genevestigatori P43558.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. Cited for: FUNCTION.
    6. "Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone."
      Rumpf S., Jentsch S.
      Mol. Cell 21:261-269(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DOMAIN OTU, INTERACTION WITH CDC48 AND DOA1, MUTAGENESIS OF CYS-120.
    7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiOTU1_YEAST
    AccessioniPrimary (citable) accession number: P43558
    Secondary accession number(s): D6VTI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2770 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3