Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein EMP47

Gene

EMP47

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the secretion of glycoproteins and in nucleus architecture and gene silencing. Required for the endoplasmic reticulum exit of EMP46.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Lectin

Enzyme and pathway databases

BioCyciYEAST:G3O-30417-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein EMP47
Alternative name(s):
47 kDa endomembrane protein
Endosomal P44 protein
Gene namesi
Name:EMP47
Ordered Locus Names:YFL048C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL048C.
SGDiS000001846. EMP47.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 412LumenalSequence analysisAdd BLAST384
Transmembranei413 – 433HelicalSequence analysisAdd BLAST21
Topological domaini434 – 445CytoplasmicSequence analysisAdd BLAST12

GO - Cellular componenti

  • endoplasmic reticulum-Golgi intermediate compartment Source: GO_Central
  • ER to Golgi transport vesicle Source: SGD
  • integral component of endoplasmic reticulum membrane Source: SGD
  • integral component of Golgi membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 281 PublicationAdd BLAST28
ChainiPRO_000002117329 – 445Protein EMP47Add BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi179 ↔ 213

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP43555.
PRIDEiP43555.

Interactioni

Subunit structurei

Homooligomers. Interacts with EMP46 in the endoplasmic reticulum membrane. Interacts with the coatomer proteins COP1, SEC21 and SEC23.1 Publication

Protein-protein interaction databases

BioGridi31099. 53 interactors.
DIPiDIP-7675N.
IntActiP43555. 38 interactors.
MINTiMINT-1355466.

Structurei

Secondary structure

1445
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi37 – 39Combined sources3
Helixi42 – 44Combined sources3
Beta strandi59 – 63Combined sources5
Beta strandi66 – 68Combined sources3
Beta strandi71 – 74Combined sources4
Beta strandi81 – 88Combined sources8
Beta strandi90 – 94Combined sources5
Beta strandi96 – 106Combined sources11
Beta strandi114 – 121Combined sources8
Beta strandi123 – 125Combined sources3
Beta strandi139 – 147Combined sources9
Beta strandi152 – 164Combined sources13
Helixi168 – 170Combined sources3
Helixi171 – 174Combined sources4
Beta strandi176 – 180Combined sources5
Beta strandi186 – 197Combined sources12
Helixi198 – 200Combined sources3
Beta strandi201 – 208Combined sources8
Beta strandi211 – 217Combined sources7
Beta strandi223 – 233Combined sources11
Helixi237 – 239Combined sources3
Beta strandi243 – 253Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A6YX-ray1.42A29-282[»]
2A6ZX-ray1.00A35-255[»]
2A70X-ray1.10A/B35-255[»]
2A71X-ray2.70A/B/C/D35-255[»]
ProteinModelPortaliP43555.
SMRiP43555.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43555.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 254L-type lectin-likePROSITE-ProRule annotationAdd BLAST219

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni430 – 433Mediates the interactions with COPI and COPII coat complexesBy similarity4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi441 – 445Di-lysine motif5

Domaini

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.By similarity

Sequence similaritiesi

Belongs to the EMP46/EMP47 family.Curated
Contains 1 L-type lectin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000053646.
HOGENOMiHOG000112365.
InParanoidiP43555.
KOiK10080.
OMAiEVREQHY.
OrthoDBiEOG092C4CI2.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016710. Emp46/Emp47.
IPR005052. Lectin_leg.
[Graphical view]
PfamiPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
PIRSFiPIRSF018136. L-type_lectin_fungi. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43555-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMMLITMKST VLLSVFTVLA TWAGLLEAHP LGDTSDASKL SSDYSLPDLI
60 70 80 90 100
NARKVPNNWQ TGEQASLEEG RIVLTSKQNS KGSLWLKQGF DLKDSFTMEW
110 120 130 140 150
TFRSVGYSGQ TDGGISFWFV QDSNVPRDKQ LYNGPVNYDG LQLLVDNNGP
160 170 180 190 200
LGPTLRGQLN DGQKPVDKTK IYDQSFASCL MGYQDSSVPS TIRVTYDLED
210 220 230 240 250
DNLLKVQVDN KVCFQTRKVR FPSGSYRIGV TAQNGAVNNN AESFEIFKMQ
260 270 280 290 300
FFNGVIEDSL IPNVNAMGQP KLITKYIDQQ TGKEKLIEKT AFDADKDKIT
310 320 330 340 350
NYELYKKLDR VEGKILANDI NALETKLNDV IKVQQELLSF MTTITKQLSS
360 370 380 390 400
KPPANNEKGT STDDAIAEDK ENFKDFLSIN QKLEKVLVEQ EKYREATKRH
410 420 430 440
GQDGPQVDEI ARKLMIWLLP LIFIMLVMAY YTFRIRQEII KTKLL
Length:445
Mass (Da):50,330
Last modified:November 1, 1995 - v1
Checksum:i3978462BC2AFCD8A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52A → T in CAA60953 (PubMed:7490292).Curated1
Sequence conflicti77K → N in CAA60953 (PubMed:7490292).Curated1
Sequence conflicti125V → I in CAA60953 (PubMed:7490292).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87622 Genomic DNA. Translation: CAA60953.1.
D50617 Genomic DNA. Translation: BAA09193.1.
AY693033 Genomic DNA. Translation: AAT93052.1.
BK006940 Genomic DNA. Translation: DAA12392.1.
PIRiS56207.
RefSeqiNP_116606.1. NM_001179919.1.

Genome annotation databases

EnsemblFungiiBAA09193; BAA09193; BAA09193.
YFL048C; YFL048C; YFL048C.
GeneIDi850496.
KEGGisce:YFL048C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87622 Genomic DNA. Translation: CAA60953.1.
D50617 Genomic DNA. Translation: BAA09193.1.
AY693033 Genomic DNA. Translation: AAT93052.1.
BK006940 Genomic DNA. Translation: DAA12392.1.
PIRiS56207.
RefSeqiNP_116606.1. NM_001179919.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A6YX-ray1.42A29-282[»]
2A6ZX-ray1.00A35-255[»]
2A70X-ray1.10A/B35-255[»]
2A71X-ray2.70A/B/C/D35-255[»]
ProteinModelPortaliP43555.
SMRiP43555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31099. 53 interactors.
DIPiDIP-7675N.
IntActiP43555. 38 interactors.
MINTiMINT-1355466.

Proteomic databases

MaxQBiP43555.
PRIDEiP43555.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09193; BAA09193; BAA09193.
YFL048C; YFL048C; YFL048C.
GeneIDi850496.
KEGGisce:YFL048C.

Organism-specific databases

EuPathDBiFungiDB:YFL048C.
SGDiS000001846. EMP47.

Phylogenomic databases

GeneTreeiENSGT00510000053646.
HOGENOMiHOG000112365.
InParanoidiP43555.
KOiK10080.
OMAiEVREQHY.
OrthoDBiEOG092C4CI2.

Enzyme and pathway databases

BioCyciYEAST:G3O-30417-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP43555.
PROiP43555.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016710. Emp46/Emp47.
IPR005052. Lectin_leg.
[Graphical view]
PfamiPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
PIRSFiPIRSF018136. L-type_lectin_fungi. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMP47_YEAST
AccessioniPrimary (citable) accession number: P43555
Secondary accession number(s): D6VTI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.