Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P43555 (EMP47_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein EMP47
Alternative name(s):
47 kDa endomembrane protein
Endosomal P44 protein
Gene names
Name:EMP47
Ordered Locus Names:YFL048C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the secretion of glycoproteins and in nucleus architecture and gene silencing. Required for the endoplasmic reticulum exit of EMP46. Ref.6 Ref.9

Subunit structure

Homooligomers. Interacts with EMP46 in the endoplasmic reticulum membrane. Interacts with the coatomer proteins COP1, SEC21 and SEC23. Ref.7

Subcellular location

Golgi apparatus membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein Ref.1 Ref.7.

Domain

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins By similarity. Ref.6

Miscellaneous

Present with 2900 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the EMP46/EMP47 family.

Contains 1 L-type lectin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.5
Chain29 – 445417Protein EMP47
PRO_0000021173

Regions

Topological domain29 – 412384Lumenal Potential
Transmembrane413 – 43321Helical; Potential
Topological domain434 – 44512Cytoplasmic Potential
Domain36 – 254219L-type lectin-like
Region430 – 4334Mediates the interactions with COPI and COPII coat complexes By similarity
Motif441 – 4455Di-lysine motif

Amino acid modifications

Disulfide bond179 ↔ 213

Experimental info

Sequence conflict521A → T in CAA60953. Ref.1
Sequence conflict771K → N in CAA60953. Ref.1
Sequence conflict1251V → I in CAA60953. Ref.1

Secondary structure

.......................................... 445
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43555 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 3978462BC2AFCD8A

FASTA44550,330
        10         20         30         40         50         60 
MMMLITMKST VLLSVFTVLA TWAGLLEAHP LGDTSDASKL SSDYSLPDLI NARKVPNNWQ 

        70         80         90        100        110        120 
TGEQASLEEG RIVLTSKQNS KGSLWLKQGF DLKDSFTMEW TFRSVGYSGQ TDGGISFWFV 

       130        140        150        160        170        180 
QDSNVPRDKQ LYNGPVNYDG LQLLVDNNGP LGPTLRGQLN DGQKPVDKTK IYDQSFASCL 

       190        200        210        220        230        240 
MGYQDSSVPS TIRVTYDLED DNLLKVQVDN KVCFQTRKVR FPSGSYRIGV TAQNGAVNNN 

       250        260        270        280        290        300 
AESFEIFKMQ FFNGVIEDSL IPNVNAMGQP KLITKYIDQQ TGKEKLIEKT AFDADKDKIT 

       310        320        330        340        350        360 
NYELYKKLDR VEGKILANDI NALETKLNDV IKVQQELLSF MTTITKQLSS KPPANNEKGT 

       370        380        390        400        410        420 
STDDAIAEDK ENFKDFLSIN QKLEKVLVEQ EKYREATKRH GQDGPQVDEI ARKLMIWLLP 

       430        440 
LIFIMLVMAY YTFRIRQEII KTKLL 

« Hide

References

« Hide 'large scale' references
[1]"The Golgi-localization of yeast Emp47p depends on its di-lysine motif but is not affected by the ret1-1 mutation in alpha-COP."
Schroeder S., Schimmoeller F., Singer-Krueger B., Riezman H.
J. Cell Biol. 131:895-912(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Partial purification and characterization of early and late endosomes from yeast. Identification of four novel proteins."
Singer-Krueger B., Frank R., Crausaz F., Riezman H.
J. Biol. Chem. 268:14376-14386(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-40.
Strain: RH732.
[6]"Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae."
Sato K., Nakano A.
Mol. Biol. Cell 13:2518-2532(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DOMAINS.
[7]"Oligomerization of a cargo receptor directs protein sorting into COPII-coated transport vesicles."
Sato K., Nakano A.
Mol. Biol. Cell 14:3055-3063(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EMP46, SUBUNIT, SUBCELLULAR LOCATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting."
Sato K., Nakano A.
J. Biol. Chem. 279:1330-1335(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Structures of the carbohydrate recognition domain of Ca2+-independent cargo receptors Emp46p and Emp47p."
Satoh T., Sato K., Kanoh A., Yamashita K., Yamada Y., Igarashi N., Kato R., Nakano A., Wakatsuki S.
J. Biol. Chem. 281:10410-10419(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1 ANGSTROMS) OF 29-282.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87622 Genomic DNA. Translation: CAA60953.1.
D50617 Genomic DNA. Translation: BAA09193.1.
AY693033 Genomic DNA. Translation: AAT93052.1.
BK006940 Genomic DNA. Translation: DAA12392.1.
PIRS56207.
RefSeqNP_116606.1. NM_001179919.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2A6YX-ray1.42A29-282[»]
2A6ZX-ray1.00A35-255[»]
2A70X-ray1.10A/B35-255[»]
2A71X-ray2.70A/B/C/D35-255[»]
ProteinModelPortalP43555.
SMRP43555. Positions 35-255.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31099. 45 interactions.
DIPDIP-7675N.
IntActP43555. 38 interactions.
MINTMINT-1355466.
STRING4932.YFL048C.

Proteomic databases

PaxDbP43555.
PeptideAtlasP43555.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFL048C; YFL048C; YFL048C.
GeneID850496.
KEGGsce:YFL048C.

Organism-specific databases

CYGDYFL048c.
SGDS000001846. EMP47.

Phylogenomic databases

eggNOGNOG68488.
GeneTreeENSGT00510000053646.
HOGENOMHOG000112365.
OMALVMAYYT.
OrthoDBEOG7N6414.

Enzyme and pathway databases

BioCycYEAST:G3O-30417-MONOMER.

Gene expression databases

GenevestigatorP43555.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR016710. L-typ_lectin_fun.
IPR005052. Lectin_leg.
[Graphical view]
PANTHERPTHR12223. PTHR12223. 1 hit.
PfamPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
PIRSFPIRSF018136. L-type_lectin_fungi. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP43555.
NextBio966182.

Entry information

Entry nameEMP47_YEAST
AccessionPrimary (citable) accession number: P43555
Secondary accession number(s): D6VTI2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references