Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P43555

- EMP47_YEAST

UniProt

P43555 - EMP47_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein EMP47

Gene

EMP47

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the secretion of glycoproteins and in nucleus architecture and gene silencing. Required for the endoplasmic reticulum exit of EMP46.2 Publications

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. glycoprotein binding Source: SGD

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Ligandi

Lectin

Enzyme and pathway databases

BioCyciYEAST:G3O-30417-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein EMP47
Alternative name(s):
47 kDa endomembrane protein
Endosomal P44 protein
Gene namesi
Name:EMP47
Ordered Locus Names:YFL048C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL048c.
SGDiS000001846. EMP47.

Subcellular locationi

GO - Cellular componenti

  1. ER to Golgi transport vesicle Source: SGD
  2. integral component of endoplasmic reticulum membrane Source: SGD
  3. integral component of Golgi membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28281 PublicationAdd
BLAST
Chaini29 – 445417Protein EMP47PRO_0000021173Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi179 ↔ 213

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP43555.
PaxDbiP43555.
PeptideAtlasiP43555.

Expressioni

Gene expression databases

GenevestigatoriP43555.

Interactioni

Subunit structurei

Homooligomers. Interacts with EMP46 in the endoplasmic reticulum membrane. Interacts with the coatomer proteins COP1, SEC21 and SEC23.1 Publication

Protein-protein interaction databases

BioGridi31099. 45 interactions.
DIPiDIP-7675N.
IntActiP43555. 38 interactions.
MINTiMINT-1355466.
STRINGi4932.YFL048C.

Structurei

Secondary structure

1
445
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi37 – 393
Helixi42 – 443
Beta strandi59 – 635
Beta strandi66 – 683
Beta strandi71 – 744
Beta strandi81 – 888
Beta strandi90 – 945
Beta strandi96 – 10611
Beta strandi114 – 1218
Beta strandi123 – 1253
Beta strandi139 – 1479
Beta strandi152 – 16413
Helixi168 – 1703
Helixi171 – 1744
Beta strandi176 – 1805
Beta strandi186 – 19712
Helixi198 – 2003
Beta strandi201 – 2088
Beta strandi211 – 2177
Beta strandi223 – 23311
Helixi237 – 2393
Beta strandi243 – 25311

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A6YX-ray1.42A29-282[»]
2A6ZX-ray1.00A35-255[»]
2A70X-ray1.10A/B35-255[»]
2A71X-ray2.70A/B/C/D35-255[»]
ProteinModelPortaliP43555.
SMRiP43555. Positions 35-255.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43555.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 412384LumenalSequence AnalysisAdd
BLAST
Topological domaini434 – 44512CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei413 – 43321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 254219L-type lectin-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni430 – 4334Mediates the interactions with COPI and COPII coat complexesBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi441 – 4455Di-lysine motif

Domaini

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.By similarity

Sequence similaritiesi

Belongs to the EMP46/EMP47 family.Curated
Contains 1 L-type lectin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG68488.
GeneTreeiENSGT00510000053646.
HOGENOMiHOG000112365.
InParanoidiP43555.
OMAiLVMAYYT.
OrthoDBiEOG7N6414.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016710. L-typ_lectin_fun.
IPR005052. Lectin_leg.
[Graphical view]
PANTHERiPTHR12223. PTHR12223. 1 hit.
PfamiPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
PIRSFiPIRSF018136. L-type_lectin_fungi. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43555 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMMLITMKST VLLSVFTVLA TWAGLLEAHP LGDTSDASKL SSDYSLPDLI
60 70 80 90 100
NARKVPNNWQ TGEQASLEEG RIVLTSKQNS KGSLWLKQGF DLKDSFTMEW
110 120 130 140 150
TFRSVGYSGQ TDGGISFWFV QDSNVPRDKQ LYNGPVNYDG LQLLVDNNGP
160 170 180 190 200
LGPTLRGQLN DGQKPVDKTK IYDQSFASCL MGYQDSSVPS TIRVTYDLED
210 220 230 240 250
DNLLKVQVDN KVCFQTRKVR FPSGSYRIGV TAQNGAVNNN AESFEIFKMQ
260 270 280 290 300
FFNGVIEDSL IPNVNAMGQP KLITKYIDQQ TGKEKLIEKT AFDADKDKIT
310 320 330 340 350
NYELYKKLDR VEGKILANDI NALETKLNDV IKVQQELLSF MTTITKQLSS
360 370 380 390 400
KPPANNEKGT STDDAIAEDK ENFKDFLSIN QKLEKVLVEQ EKYREATKRH
410 420 430 440
GQDGPQVDEI ARKLMIWLLP LIFIMLVMAY YTFRIRQEII KTKLL
Length:445
Mass (Da):50,330
Last modified:November 1, 1995 - v1
Checksum:i3978462BC2AFCD8A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521A → T in CAA60953. (PubMed:7490292)Curated
Sequence conflicti77 – 771K → N in CAA60953. (PubMed:7490292)Curated
Sequence conflicti125 – 1251V → I in CAA60953. (PubMed:7490292)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87622 Genomic DNA. Translation: CAA60953.1.
D50617 Genomic DNA. Translation: BAA09193.1.
AY693033 Genomic DNA. Translation: AAT93052.1.
BK006940 Genomic DNA. Translation: DAA12392.1.
PIRiS56207.
RefSeqiNP_116606.1. NM_001179919.1.

Genome annotation databases

EnsemblFungiiYFL048C; YFL048C; YFL048C.
GeneIDi850496.
KEGGisce:YFL048C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87622 Genomic DNA. Translation: CAA60953.1 .
D50617 Genomic DNA. Translation: BAA09193.1 .
AY693033 Genomic DNA. Translation: AAT93052.1 .
BK006940 Genomic DNA. Translation: DAA12392.1 .
PIRi S56207.
RefSeqi NP_116606.1. NM_001179919.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2A6Y X-ray 1.42 A 29-282 [» ]
2A6Z X-ray 1.00 A 35-255 [» ]
2A70 X-ray 1.10 A/B 35-255 [» ]
2A71 X-ray 2.70 A/B/C/D 35-255 [» ]
ProteinModelPortali P43555.
SMRi P43555. Positions 35-255.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31099. 45 interactions.
DIPi DIP-7675N.
IntActi P43555. 38 interactions.
MINTi MINT-1355466.
STRINGi 4932.YFL048C.

Proteomic databases

MaxQBi P43555.
PaxDbi P43555.
PeptideAtlasi P43555.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL048C ; YFL048C ; YFL048C .
GeneIDi 850496.
KEGGi sce:YFL048C.

Organism-specific databases

CYGDi YFL048c.
SGDi S000001846. EMP47.

Phylogenomic databases

eggNOGi NOG68488.
GeneTreei ENSGT00510000053646.
HOGENOMi HOG000112365.
InParanoidi P43555.
OMAi LVMAYYT.
OrthoDBi EOG7N6414.

Enzyme and pathway databases

BioCyci YEAST:G3O-30417-MONOMER.

Miscellaneous databases

EvolutionaryTracei P43555.
NextBioi 966182.

Gene expression databases

Genevestigatori P43555.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR016710. L-typ_lectin_fun.
IPR005052. Lectin_leg.
[Graphical view ]
PANTHERi PTHR12223. PTHR12223. 1 hit.
Pfami PF03388. Lectin_leg-like. 1 hit.
[Graphical view ]
PIRSFi PIRSF018136. L-type_lectin_fungi. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Golgi-localization of yeast Emp47p depends on its di-lysine motif but is not affected by the ret1-1 mutation in alpha-COP."
    Schroeder S., Schimmoeller F., Singer-Krueger B., Riezman H.
    J. Cell Biol. 131:895-912(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Partial purification and characterization of early and late endosomes from yeast. Identification of four novel proteins."
    Singer-Krueger B., Frank R., Crausaz F., Riezman H.
    J. Biol. Chem. 268:14376-14386(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-40.
    Strain: RH732.
  6. "Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae."
    Sato K., Nakano A.
    Mol. Biol. Cell 13:2518-2532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAINS.
  7. "Oligomerization of a cargo receptor directs protein sorting into COPII-coated transport vesicles."
    Sato K., Nakano A.
    Mol. Biol. Cell 14:3055-3063(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EMP46, SUBUNIT, SUBCELLULAR LOCATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting."
    Sato K., Nakano A.
    J. Biol. Chem. 279:1330-1335(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Structures of the carbohydrate recognition domain of Ca2+-independent cargo receptors Emp46p and Emp47p."
    Satoh T., Sato K., Kanoh A., Yamashita K., Yamada Y., Igarashi N., Kato R., Nakano A., Wakatsuki S.
    J. Biol. Chem. 281:10410-10419(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1 ANGSTROMS) OF 29-282.

Entry informationi

Entry nameiEMP47_YEAST
AccessioniPrimary (citable) accession number: P43555
Secondary accession number(s): D6VTI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3