Protein EMP47 precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P43555 (EMP47_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein EMP47

Alternative name(s):
47 kDa endomembrane protein
Endosomal P44 protein

Gene names

Name:	EMP47
Ordered Locus Names:	YFL048C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the secretion of glycoproteins and in nucleus architecture and gene silencing. Required for the endoplasmic reticulum exit of EMP46. Ref.6 Ref.9
Subunit structure	Homooligomers. Interacts with EMP46 in the endoplasmic reticulum membrane. Interacts with the coatomer proteins COP1, SEC21 and SEC23. Ref.7
Subcellular location	Golgi apparatus membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein Ref.1 Ref.7.
Domain	The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins By similarity. Ref.6
Miscellaneous	Present with 2900 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the EMP46/EMP47 family. Contains 1 L-type lectin-like domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Lectin
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from physical interaction PubMed 11157978. Source: SGD
Cellular_component	ER to Golgi transport vesicle Inferred from direct assay PubMed 11157978. Source: SGD fungal-type vacuole membrane Inferred from direct assay PubMed 14562095. Source: SGD integral to Golgi membrane Inferred from direct assay Ref.7. Source: SGD integral to endoplasmic reticulum membrane Inferred from direct assay Ref.7. Source: SGD
Molecular_function	glycoprotein binding Inferred from sequence alignment Ref.10. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

Ref.5

Chain

29 – 445

417

Protein EMP47

PRO_0000021173

Regions

Topological domain

29 – 412

384

Lumenal Potential

Transmembrane

413 – 433

Helical; Potential

Topological domain

434 – 445

Cytoplasmic Potential

Domain

36 – 254

219

L-type lectin-like

Region

430 – 433

Mediates the interactions with COPI and COPII coat complexes By similarity

Motif

441 – 445

Di-lysine motif

Amino acid modifications

Disulfide bond

179 ↔ 213

Experimental info

Sequence conflict

A → T in CAA60953. Ref.1

Sequence conflict

K → N in CAA60953. Ref.1

Sequence conflict

125

V → I in CAA60953. Ref.1

Secondary structure

445

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P43555 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 3978462BC2AFCD8A
Show »

FASTA

445

50,330

        10         20         30         40         50         60 
MMMLITMKST VLLSVFTVLA TWAGLLEAHP LGDTSDASKL SSDYSLPDLI NARKVPNNWQ 

        70         80         90        100        110        120 
TGEQASLEEG RIVLTSKQNS KGSLWLKQGF DLKDSFTMEW TFRSVGYSGQ TDGGISFWFV 

       130        140        150        160        170        180 
QDSNVPRDKQ LYNGPVNYDG LQLLVDNNGP LGPTLRGQLN DGQKPVDKTK IYDQSFASCL 

       190        200        210        220        230        240 
MGYQDSSVPS TIRVTYDLED DNLLKVQVDN KVCFQTRKVR FPSGSYRIGV TAQNGAVNNN 

       250        260        270        280        290        300 
AESFEIFKMQ FFNGVIEDSL IPNVNAMGQP KLITKYIDQQ TGKEKLIEKT AFDADKDKIT 

       310        320        330        340        350        360 
NYELYKKLDR VEGKILANDI NALETKLNDV IKVQQELLSF MTTITKQLSS KPPANNEKGT 

       370        380        390        400        410        420 
STDDAIAEDK ENFKDFLSIN QKLEKVLVEQ EKYREATKRH GQDGPQVDEI ARKLMIWLLP 

       430        440 
LIFIMLVMAY YTFRIRQEII KTKLL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Golgi-localization of yeast Emp47p depends on its di-lysine motif but is not affected by the ret1-1 mutation in alpha-COP." Schroeder S., Schimmoeller F., Singer-Krueger B., Riezman H. J. Cell Biol. 131:895-912(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Partial purification and characterization of early and late endosomes from yeast. Identification of four novel proteins." Singer-Krueger B., Frank R., Crausaz F., Riezman H. J. Biol. Chem. 268:14376-14386(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-40. Strain: RH732.
[6]	"Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae." Sato K., Nakano A. Mol. Biol. Cell 13:2518-2532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DOMAINS.
[7]	"Oligomerization of a cargo receptor directs protein sorting into COPII-coated transport vesicles." Sato K., Nakano A. Mol. Biol. Cell 14:3055-3063(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH EMP46, SUBUNIT, SUBCELLULAR LOCATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting." Sato K., Nakano A. J. Biol. Chem. 279:1330-1335(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Structures of the carbohydrate recognition domain of Ca2+-independent cargo receptors Emp46p and Emp47p." Satoh T., Sato K., Kanoh A., Yamashita K., Yamada Y., Igarashi N., Kato R., Nakano A., Wakatsuki S. J. Biol. Chem. 281:10410-10419(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1 ANGSTROMS) OF 29-282.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X87622 Genomic DNA. Translation: CAA60953.1.
D50617 Genomic DNA. Translation: BAA09193.1.
AY693033 Genomic DNA. Translation: AAT93052.1.
BK006940 Genomic DNA. Translation: DAA12392.1.

PIR

S56207.

RefSeq

NP_116606.1. NM_001179919.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A6Y	X-ray	1.42	A	29-282	[»]
2A6Z	X-ray	1.00	A	35-255	[»]
2A70	X-ray	1.10	A/B	35-255	[»]
2A71	X-ray	2.70	A/B/C/D	35-255	[»]

ProteinModelPortal

P43555.

SMR

P43555. Positions 35-255.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-7675N.

IntAct

P43555. 29 interactions.

MINT

MINT-1355466.

STRING

4932.YFL048C.

Proteomic databases

PaxDb

P43555.

PeptideAtlas

P43555.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFL048C; YFL048C; YFL048C.

GeneID

850496.

KEGG

sce:YFL048C.

Organism-specific databases

CYGD

YFL048c.

SGD

S000001846. EMP47.

Phylogenomic databases

eggNOG

NOG68488.

GeneTree

ENSGT00510000053646.

HOGENOM

HOG000112365.

OMA

LVMAYYT.

OrthoDB

EOG45TGX3.

Gene expression databases

Genevestigator

P43555.

GermOnline

YFL048C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.60.120.200. 1 hit.

InterPro

IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR016710. L-typ_lectin_fun.
IPR005052. Lectin_leg.
[Graphical view]

PANTHER

PTHR12223. PTHR12223. 1 hit.

Pfam

PF03388. Lectin_leg-like. 1 hit.
[Graphical view]

PIRSF

PIRSF018136. L-type_lectin_fungi. 1 hit.

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

PROSITE

PS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P43555.

NextBio

966182.

Entry information

Entry name

EMP47_YEAST

Accession

Primary (citable) accession number: P43555
Secondary accession number(s): D6VTI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents