ID   SWP82_YEAST             Reviewed;         623 AA.
AC   P43554; D6VTI1;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 165.
DE   RecName: Full=SWI/SNF global transcription activator complex subunit SWP82;
GN   Name=SWP82; OrderedLocusNames=YFL049W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7670463; DOI=10.1038/ng0795-261;
RA   Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA   Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA   Eki T.;
RT   "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT   cerevisiae.";
RL   Nat. Genet. 10:261-268(1995).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, AND ELECTRON MICROSCOPY OF
RP   THE SWI/SNF COMPLEX.
RX   PubMed=12524530; DOI=10.1038/nsb888;
RA   Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L.,
RA   Peterson C.L.;
RT   "Structural analysis of the yeast SWI/SNF chromatin remodeling complex.";
RL   Nat. Struct. Biol. 10:141-145(2003).
RN   [6]
RP   IDENTIFICATION IN THE SWI/SNF COMPLEX, AND INTERACTION WITH SWI3 AND SNF12.
RX   PubMed=16204215; DOI=10.1534/genetics.105.047589;
RA   Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.;
RT   "The RSC chromatin remodeling complex bears an essential fungal-specific
RT   protein module with broad functional roles.";
RL   Genetics 172:795-809(2006).
CC   -!- FUNCTION: Involved in transcriptional activation. Component of the
CC       SWI/SNF complex, an ATP-dependent chromatin remodeling complex, which
CC       is required for the positive and negative regulation of gene expression
CC       of a large number of genes. It changes chromatin structure by altering
CC       DNA-histone contacts within a nucleosome, leading eventually to a
CC       change in nucleosome position, thus facilitating or repressing binding
CC       of gene-specific transcription factors.
CC   -!- SUBUNIT: Interacts with SWI3 and SNF12. Component of the SWI/SNF global
CC       transcription activator complex. The 1.14 MDa SWI/SNF complex is
CC       composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2,
CC       SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3,
CC       SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.
CC       {ECO:0000269|PubMed:16204215}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 3180 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the RSC7/SWP82 family. SWP82 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D50617; BAA09192.1; -; Genomic_DNA.
DR   EMBL; BK006940; DAA12391.1; -; Genomic_DNA.
DR   PIR; S56206; S56206.
DR   RefSeq; NP_116605.1; NM_001179918.1.
DR   PDB; 7C4J; EM; 2.89 A; E=1-623.
DR   PDB; 7EGM; EM; 3.60 A; J=1-623.
DR   PDB; 7EGP; EM; 6.90 A; J=1-623.
DR   PDBsum; 7C4J; -.
DR   PDBsum; 7EGM; -.
DR   PDBsum; 7EGP; -.
DR   AlphaFoldDB; P43554; -.
DR   EMDB; EMD-30285; -.
DR   EMDB; EMD-31136; -.
DR   EMDB; EMD-31137; -.
DR   SMR; P43554; -.
DR   BioGRID; 31098; 168.
DR   ComplexPortal; CPX-1150; SWI/SNF chromatin remodelling complex.
DR   DIP; DIP-4202N; -.
DR   IntAct; P43554; 17.
DR   MINT; P43554; -.
DR   STRING; 4932.YFL049W; -.
DR   MaxQB; P43554; -.
DR   PaxDb; 4932-YFL049W; -.
DR   PeptideAtlas; P43554; -.
DR   EnsemblFungi; YFL049W_mRNA; YFL049W; YFL049W.
DR   GeneID; 850495; -.
DR   KEGG; sce:YFL049W; -.
DR   AGR; SGD:S000001845; -.
DR   SGD; S000001845; SWP82.
DR   VEuPathDB; FungiDB:YFL049W; -.
DR   eggNOG; ENOG502QVDZ; Eukaryota.
DR   HOGENOM; CLU_493537_0_0_1; -.
DR   InParanoid; P43554; -.
DR   OMA; YWQYKAG; -.
DR   OrthoDB; 1967408at2759; -.
DR   BioCyc; YEAST:G3O-30416-MONOMER; -.
DR   Reactome; R-SCE-4551638; SUMOylation of chromatin organization proteins.
DR   BioGRID-ORCS; 850495; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P43554; -.
DR   Proteomes; UP000002311; Chromosome VI.
DR   RNAct; P43554; Protein.
DR   GO; GO:0000785; C:chromatin; NAS:ComplexPortal.
DR   GO; GO:0005829; C:cytosol; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0016586; C:RSC-type complex; IBA:GO_Central.
DR   GO; GO:0016514; C:SWI/SNF complex; IDA:UniProtKB.
DR   GO; GO:0031490; F:chromatin DNA binding; IBA:GO_Central.
DR   GO; GO:0006338; P:chromatin remodeling; IDA:ComplexPortal.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:ComplexPortal.
DR   InterPro; IPR013933; CRC_Rsc7/Swp82.
DR   Pfam; PF08624; CRC_subunit; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Chromatin regulator; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..623
FT                   /note="SWI/SNF global transcription activator complex
FT                   subunit SWP82"
FT                   /id="PRO_0000076350"
FT   HELIX           32..49
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   STRAND          53..56
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           122..130
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           188..194
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           196..198
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   STRAND          200..202
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   STRAND          205..210
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           231..240
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           283..295
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   STRAND          316..318
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           328..334
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   STRAND          443..446
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           451..460
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           463..465
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           466..501
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   HELIX           512..524
FT                   /evidence="ECO:0007829|PDB:7C4J"
FT   STRAND          551..553
FT                   /evidence="ECO:0007829|PDB:7C4J"
SQ   SEQUENCE   623 AA;  70276 MW;  A8AC00CAC8F0ED0E CRC64;
     MLGEDEGNTV LEKGNNPSVK QGEVGAVFIV PKILIREHER VILKQILQIL DQDELVQPPL
     DKFPYKKLEL PKYIDELKTR DATNTSYKMI QLDAYGEKKV GSNGELFGGR HYLFNTFTFT
     AHMGVLLVLL QDVIKVLYQS NATHDEDEFI VQHDQILVME TSEEQTKFLA KNGVIPEESK
     GSFKYITARS AFVEFGASVI AGGQRIVDDY WESLAKKQNL SSHQRVFKLS TNLISKISLL
     RPSFQNNRIS NANEISANTN NTCTISTSKF ESQYPIVTEQ PSAEIREAYI ENFAKGEHIS
     AIVPGQSISG TLELSAQFRV PRYHSKNSFQ QALQMKAMDI PIGRHEELLA QYESQAPDGS
     ASISLPNHIP SVNPSNKPIK RMLSSILDIN VSSSKNKKSE ENEMIKPMNK GQHKNNTSLN
     INGWKFESLP LKSAENSGKQ QYYRGLPLYE KNTLLERLKQ LTPNEIKELE HLHDAVFVNT
     GLQNVRKVRT KKWKKYWQYK AGIPIGLKRS QLDEFKNKYL KDVLAQTSVT TNFNEITNTD
     ETITTKRVPN PNFLGNCNIK DFKPPYIYSH VNKVPQNVAG DKTAVKLDTE VKNTNANPVV
     ATDPVAAKPD NLANFSNEVA MNN
//