P43553 (ALR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Magnesium transporter ALR2

Alternative name(s):
Aluminum resistance protein 2

Gene names

Name:	ALR2
Ordered Locus Names:	YFL050C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plasma membrane magnesium transporter. Ref.3
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 2130 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	magnesium ion transport Inferred from direct assay PubMed 12167543. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD plasma membrane Inferred from mutant phenotype Ref.3. Source: SGD
Molecular_function	inorganic cation transmembrane transporter activity Inferred from mutant phenotype Ref.3. Source: SGD metal ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 858	858	Magnesium transporter ALR2		PRO_0000201536
Regions
	Topological domain	1 – 741	741	Cytoplasmic Potential
	Transmembrane	742 – 762	21	Helical; Potential
	Topological domain	763 – 771	9	Extracellular Potential
	Transmembrane	772 – 792	21	Helical; Potential
	Topological domain	793 – 858	66	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P43553 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 7AAFEC6086FF0618	FASTA	858	96,684
	10 20 30 40 50 60 MSSLSTSFDS SSDLPRSKSV DNTAASMKTG KYPKLENYRQ YSDAQPIRHE ALALKVDETK 70 80 90 100 110 120 DSRHKFSSSN GENSGVENGG YVEKTNISTS GRMDFEGEAE AEAVKRYQLR SFALLSSNAR 130 140 150 160 170 180 PSRLAKSETH QKQIHVESIA PSLPKNAALE RGHDTALPAG TSSNRCNLEA SSSARTFTSA 190 200 210 220 230 240 RKASLVSAIF ETSAESEHGT HPKQAKLKRR TYSTISTHSS VNPTTLLTRT ASQKSDMGND 250 260 270 280 290 300 TRRIKPLRMD SRVSFHSEIS QASRDSQETE EDVCFPMFRL LHTRVNGVDF DELEEYAQIS 310 320 330 340 350 360 NAERNLSLAN HQRHSERTYN HTDQDTGFTN SASTSGSSAA LKYTPEISRT LEKNCSVNEM 370 380 390 400 410 420 YVSENNESVR EDDKPDLHPD VTFGRNKIEG EKEGNDSSYS RAYYTLQNTE YQIPSRFSFF 430 440 450 460 470 480 RSESDETVHA SDIPSLISEG QTFYELFKGG DPTWWLDCSC PTDDEMRCIA KTFGIHPLTA 490 500 510 520 530 540 EDIRMQETRE KVELFKSYYF VCFHTFENDK ESENYLEPIN VYIVVFRSGV LTFHFDPISH 550 560 570 580 590 600 CANVRRRVRQ LRDYVSVNSD WLCYALIDDI TDSFAPVIQS IEYEADSIDD SVFMTRDMDF 610 620 630 640 650 660 AAMLQRIGES RRKTMTLMRL LSGKADVIKM FAKRCQDETN GIGPVLKSQT NMVNLQAEQE 670 680 690 700 710 720 NVNQNNSNNQ ISLSNSYMQT TSQPRGDIAL YLGDIQDHLL TMFQNLLAYE KIFSRSHANY 730 740 750 760 770 780 LAQLQVESFN SNNKVTEMLG KVTMLGTMLV PLNVITGLFG MNVKVPGRNG SIAWWYGILG 790 800 810 820 830 840 VLLLLAVISW FLASYWIKKI DPPATLNEAA GSGAKSVISS FLPKRDKRFN DDSKNGNARV 850 GVRRKSTVSL PSRYSRYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Overexpression of the Saccharomyces cerevisiae magnesium transport system confers resistance to aluminum ion." MacDiarmid C.W., Gardner R.C. J. Biol. Chem. 273:1727-1732(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09191.1. BK006940 Genomic DNA. Translation: DAA12390.1.
PIR	S56205.
RefSeq	NP_116604.1. NM_001179917.1.
3D structure databases
ProteinModelPortal	P43553.
SMR	P43553. Positions 413-799.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4581N.
MINT	MINT-492745.
STRING	4932.YFL050C.
Proteomic databases
PaxDb	P43553.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL050C; YFL050C; YFL050C.
GeneID	850494.
KEGG	sce:YFL050C.
Organism-specific databases
CYGD	YFL050c.
SGD	S000001844. ALR2.
Phylogenomic databases
eggNOG	COG0598.
GeneTree	ENSGT00390000009621.
HOGENOM	HOG000171963.
KO	K16073.
OrthoDB	EOG4SXRMW.
Gene expression databases
Genevestigator	P43553.
GermOnline	YFL050C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002523. MgTranspt_CorA/ZnTranspt_ZntB. [Graphical view]
Pfam	PF01544. CorA. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	966176.

Entry information

Entry name

ALR2_YEAST

Accession

Primary (citable) accession number: P43553
Secondary accession number(s): D6VTI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program