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Protein

Dihydroxyacetone kinase 2

Gene

DAK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.By similarity

Catalytic activityi

ATP + glycerone = ADP + glycerone phosphate.
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.

Pathwayi: glycerol fermentation

This protein is involved in step 2 of the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Dihydroxyacetone kinase 1 (DAK1), Dihydroxyacetone kinase 2 (DAK2)
This subpathway is part of the pathway glycerol fermentation, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route), the pathway glycerol fermentation and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109SubstrateBy similarity1
Binding sitei114SubstrateBy similarity1
Active sitei223Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi413 – 416ATPBy similarity4
Nucleotide bindingi459 – 460ATPBy similarity2
Nucleotide bindingi511 – 512ATPBy similarity2
Nucleotide bindingi572 – 574ATPBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glycerone kinase activity Source: SGD
  • triokinase activity Source: UniProtKB-EC

GO - Biological processi

  • anaerobic glycerol catabolic process Source: UniProtKB-UniPathway
  • cellular response to toxic substance Source: SGD
  • glycerol catabolic process Source: SGD

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycerol metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YFL053W-MONOMER
YEAST:YFL053W-MONOMER
BRENDAi2.7.1.29 984
ReactomeiR-SCE-70350 Fructose catabolism
UniPathwayiUPA00617; UER00669

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxyacetone kinase 2 (EC:2.7.1.28, EC:2.7.1.29)
Short name:
DHA kinase 2
Alternative name(s):
Glycerone kinase 2
Triokinase 2
Triose kinase 2
Gene namesi
Name:DAK2
Ordered Locus Names:YFL053W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL053W
SGDiS000001841 DAK2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001215231 – 591Dihydroxyacetone kinase 2Add BLAST591

Proteomic databases

PaxDbiP43550
PRIDEiP43550

PTM databases

iPTMnetiP43550

Interactioni

Protein-protein interaction databases

BioGridi31094, 40 interactors
DIPiDIP-6539N
IntActiP43550, 2 interactors
MINTiP43550
STRINGi4932.YFL053W

Structurei

3D structure databases

ProteinModelPortaliP43550
SMRiP43550
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 344DhaKPROSITE-ProRule annotationAdd BLAST337
Domaini384 – 587DhaLPROSITE-ProRule annotationAdd BLAST204

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 61Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000015415
HOGENOMiHOG000234158
InParanoidiP43550
KOiK00863
OMAiSTKGMEA
OrthoDBiEOG092C1OGC

Family and domain databases

Gene3Di1.25.40.340, 1 hit
InterProiView protein in InterPro
IPR012734 DhaK_ATP
IPR004006 DhaK_dom
IPR004007 DhaL_dom
IPR036117 DhaL_dom_sf
PfamiView protein in Pfam
PF02733 Dak1, 1 hit
PF02734 Dak2, 1 hit
SMARTiView protein in SMART
SM01120 Dak2, 1 hit
SUPFAMiSSF101473 SSF101473, 1 hit
TIGRFAMsiTIGR02361 dak_ATP, 1 hit
PROSITEiView protein in PROSITE
PS51481 DHAK, 1 hit
PS51480 DHAL, 1 hit

Sequencei

Sequence statusi: Complete.

P43550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHKQFKSDG NIVTPYLLGL ARSNPGLTVI KHDRVVFRTA SAPNSGNPPK
60 70 80 90 100
VSLVSGGGSG HEPTHAGFVG EGALDAIAAG AIFASPSTKQ IYSAIKAVES
110 120 130 140 150
PKGTLIIVKN YTGDIIHFGL AAERAKAAGM KVELVAVGDD VSVGKKKGSL
160 170 180 190 200
VGRRGLGATV LVHKIAGAAA SHGLELAEVA EVAQSVVDNS VTIAASLDHC
210 220 230 240 250
TVPGHKPEAI LGENEYEIGM GIHNESGTYK SSPLPSISEL VSQMLPLLLD
260 270 280 290 300
EDEDRSYVKF EPKEDVVLMV NNMGGMSNLE LGYAAEVISE QLIDKYQIVP
310 320 330 340 350
KRTITGAFIT ALNGPGFGIT LMNASKAGGD ILKYFDYPTT ASGWNQMYHS
360 370 380 390 400
AKDWEVLAKG QVPTAPSLKT LRNEKGSGVK ADYDTFAKIL LAGIAKINEV
410 420 430 440 450
EPKVTWYDTI AGDGDCGTTL VSGGEALEEA IKNHTLRLED AALGIEDIAY
460 470 480 490 500
MVEDSMGGTS GGLYSIYLSA LAQGVRDSGD KELTAETFKK ASNVALDALY
510 520 530 540 550
KYTRARPGYR TLIDALQPFV EALKAGKGPR AAAQAAYDGA EKTRKMDALV
560 570 580 590
GRASYVAKEE LRKLDSEGGL PDPGAVGLAA LLDGFVTAAG Y
Length:591
Mass (Da):62,135
Last modified:November 1, 1995 - v1
Checksum:iEE317EC120C0E313
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA Translation: BAA09188.1
BK006940 Genomic DNA Translation: DAA12387.1
PIRiS56202
RefSeqiNP_116602.1, NM_001179914.1

Genome annotation databases

EnsemblFungiiBAA09188; BAA09188; BAA09188
YFL053W; YFL053W; YFL053W
GeneIDi850491
KEGGisce:YFL053W

Similar proteinsi

Entry informationi

Entry nameiDAK2_YEAST
AccessioniPrimary (citable) accession number: P43550
Secondary accession number(s): D6VTH7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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