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Protein

Dihydroxyacetone kinase 2

Gene

DAK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.By similarity

Catalytic activityi

ATP + glycerone = ADP + glycerone phosphate.
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.

Pathwayi: glycerol fermentation

This protein is involved in step 2 of the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Dihydroxyacetone kinase 1 (DAK1), Dihydroxyacetone kinase 2 (DAK2)
This subpathway is part of the pathway glycerol fermentation, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route), the pathway glycerol fermentation and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091SubstrateBy similarity
Binding sitei114 – 1141SubstrateBy similarity
Active sitei223 – 2231Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi413 – 4164ATPBy similarity
Nucleotide bindingi459 – 4602ATPBy similarity
Nucleotide bindingi511 – 5122ATPBy similarity
Nucleotide bindingi572 – 5743ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glycerone kinase activity Source: SGD
  • triokinase activity Source: UniProtKB-EC

GO - Biological processi

  • anaerobic glycerol catabolic process Source: UniProtKB-UniPathway
  • cellular response to toxic substance Source: SGD
  • glycerol catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16997.
YEAST:YFL053W-MONOMER.
BRENDAi2.7.1.29. 984.
ReactomeiR-SCE-70350. Fructose catabolism.
UniPathwayiUPA00617; UER00669.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxyacetone kinase 2 (EC:2.7.1.28, EC:2.7.1.29)
Short name:
DHA kinase 2
Alternative name(s):
Glycerone kinase 2
Triokinase 2
Triose kinase 2
Gene namesi
Name:DAK2
Ordered Locus Names:YFL053W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL053W.
SGDiS000001841. DAK2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591Dihydroxyacetone kinase 2PRO_0000121523Add
BLAST

PTM databases

iPTMnetiP43550.

Interactioni

Protein-protein interaction databases

BioGridi31094. 16 interactions.
DIPiDIP-6539N.
IntActiP43550. 1 interaction.
MINTiMINT-688910.

Structurei

3D structure databases

ProteinModelPortaliP43550.
SMRiP43550. Positions 9-525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 344337DhaKPROSITE-ProRule annotationAdd
BLAST
Domaini384 – 587204DhaLPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 614Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 DhaK domain.PROSITE-ProRule annotation
Contains 1 DhaL domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000015415.
HOGENOMiHOG000234158.
InParanoidiP43550.
KOiK00863.
OMAiVCALPGQ.
OrthoDBiEOG092C1OGC.

Family and domain databases

InterProiIPR012734. DhaK_ATP.
IPR004006. DhaK_dom.
IPR004007. DhaL_dom.
[Graphical view]
PfamiPF02733. Dak1. 1 hit.
PF02734. Dak2. 1 hit.
[Graphical view]
SMARTiSM01120. Dak2. 1 hit.
[Graphical view]
SUPFAMiSSF101473. SSF101473. 1 hit.
TIGRFAMsiTIGR02361. dak_ATP. 1 hit.
PROSITEiPS51481. DHAK. 1 hit.
PS51480. DHAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHKQFKSDG NIVTPYLLGL ARSNPGLTVI KHDRVVFRTA SAPNSGNPPK
60 70 80 90 100
VSLVSGGGSG HEPTHAGFVG EGALDAIAAG AIFASPSTKQ IYSAIKAVES
110 120 130 140 150
PKGTLIIVKN YTGDIIHFGL AAERAKAAGM KVELVAVGDD VSVGKKKGSL
160 170 180 190 200
VGRRGLGATV LVHKIAGAAA SHGLELAEVA EVAQSVVDNS VTIAASLDHC
210 220 230 240 250
TVPGHKPEAI LGENEYEIGM GIHNESGTYK SSPLPSISEL VSQMLPLLLD
260 270 280 290 300
EDEDRSYVKF EPKEDVVLMV NNMGGMSNLE LGYAAEVISE QLIDKYQIVP
310 320 330 340 350
KRTITGAFIT ALNGPGFGIT LMNASKAGGD ILKYFDYPTT ASGWNQMYHS
360 370 380 390 400
AKDWEVLAKG QVPTAPSLKT LRNEKGSGVK ADYDTFAKIL LAGIAKINEV
410 420 430 440 450
EPKVTWYDTI AGDGDCGTTL VSGGEALEEA IKNHTLRLED AALGIEDIAY
460 470 480 490 500
MVEDSMGGTS GGLYSIYLSA LAQGVRDSGD KELTAETFKK ASNVALDALY
510 520 530 540 550
KYTRARPGYR TLIDALQPFV EALKAGKGPR AAAQAAYDGA EKTRKMDALV
560 570 580 590
GRASYVAKEE LRKLDSEGGL PDPGAVGLAA LLDGFVTAAG Y
Length:591
Mass (Da):62,135
Last modified:November 1, 1995 - v1
Checksum:iEE317EC120C0E313
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09188.1.
BK006940 Genomic DNA. Translation: DAA12387.1.
PIRiS56202.
RefSeqiNP_116602.1. NM_001179914.1.

Genome annotation databases

EnsemblFungiiBAA09188; BAA09188; BAA09188.
YFL053W; YFL053W; YFL053W.
GeneIDi850491.
KEGGisce:YFL053W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09188.1.
BK006940 Genomic DNA. Translation: DAA12387.1.
PIRiS56202.
RefSeqiNP_116602.1. NM_001179914.1.

3D structure databases

ProteinModelPortaliP43550.
SMRiP43550. Positions 9-525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31094. 16 interactions.
DIPiDIP-6539N.
IntActiP43550. 1 interaction.
MINTiMINT-688910.

PTM databases

iPTMnetiP43550.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09188; BAA09188; BAA09188.
YFL053W; YFL053W; YFL053W.
GeneIDi850491.
KEGGisce:YFL053W.

Organism-specific databases

EuPathDBiFungiDB:YFL053W.
SGDiS000001841. DAK2.

Phylogenomic databases

GeneTreeiENSGT00390000015415.
HOGENOMiHOG000234158.
InParanoidiP43550.
KOiK00863.
OMAiVCALPGQ.
OrthoDBiEOG092C1OGC.

Enzyme and pathway databases

UniPathwayiUPA00617; UER00669.
BioCyciMetaCyc:MONOMER-16997.
YEAST:YFL053W-MONOMER.
BRENDAi2.7.1.29. 984.
ReactomeiR-SCE-70350. Fructose catabolism.

Miscellaneous databases

PROiP43550.

Family and domain databases

InterProiIPR012734. DhaK_ATP.
IPR004006. DhaK_dom.
IPR004007. DhaL_dom.
[Graphical view]
PfamiPF02733. Dak1. 1 hit.
PF02734. Dak2. 1 hit.
[Graphical view]
SMARTiSM01120. Dak2. 1 hit.
[Graphical view]
SUPFAMiSSF101473. SSF101473. 1 hit.
TIGRFAMsiTIGR02361. dak_ATP. 1 hit.
PROSITEiPS51481. DHAK. 1 hit.
PS51480. DHAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAK2_YEAST
AccessioniPrimary (citable) accession number: P43550
Secondary accession number(s): D6VTH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.