Reviewed, UniProtKB/Swiss-Prot P43550 (DAK2_YEAST)

Names and origin

Protein names

Recommended name:
    Dihydroxyacetone kinase 2
      Short name=DHA kinase 2
    EC=2.7.1.29
Alternative name(s):
    Glycerone kinase 2

Gene names

Name:	DAK2
Ordered Locus Names:	YFL053W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + glycerone = ADP + glycerone phosphate.
Pathway	Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.
Sequence similarities	Belongs to the dihydroxyacetone kinase (DAK) family. Contains 1 DAK1 (dihydroxyacetone kinase subunit 1) domain. Contains 1 DAK2 (dihydroxyacetone kinase subunit 2) domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from mutant phenotype. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerone kinase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Dihydroxyacetone kinase 2		PRO_0000121523
Regions
	Domain	17 – 345	329	DAK1
	Domain	410 – 587	178	DAK2
Amino acid modifications
	Modified residue	242	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P43550-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: EE317EC120C0E313	FASTA	591	62,135
	10 20 30 40 50 60 MSHKQFKSDG NIVTPYLLGL ARSNPGLTVI KHDRVVFRTA SAPNSGNPPK VSLVSGGGSG 70 80 90 100 110 120 HEPTHAGFVG EGALDAIAAG AIFASPSTKQ IYSAIKAVES PKGTLIIVKN YTGDIIHFGL 130 140 150 160 170 180 AAERAKAAGM KVELVAVGDD VSVGKKKGSL VGRRGLGATV LVHKIAGAAA SHGLELAEVA 190 200 210 220 230 240 EVAQSVVDNS VTIAASLDHC TVPGHKPEAI LGENEYEIGM GIHNESGTYK SSPLPSISEL 250 260 270 280 290 300 VSQMLPLLLD EDEDRSYVKF EPKEDVVLMV NNMGGMSNLE LGYAAEVISE QLIDKYQIVP 310 320 330 340 350 360 KRTITGAFIT ALNGPGFGIT LMNASKAGGD ILKYFDYPTT ASGWNQMYHS AKDWEVLAKG 370 380 390 400 410 420 QVPTAPSLKT LRNEKGSGVK ADYDTFAKIL LAGIAKINEV EPKVTWYDTI AGDGDCGTTL 430 440 450 460 470 480 VSGGEALEEA IKNHTLRLED AALGIEDIAY MVEDSMGGTS GGLYSIYLSA LAQGVRDSGD 490 500 510 520 530 540 KELTAETFKK ASNVALDALY KYTRARPGYR TLIDALQPFV EALKAGKGPR AAAQAAYDGA 550 560 570 580 590 EKTRKMDALV GRASYVAKEE LRKLDSEGGL PDPGAVGLAA LLDGFVTAAG Y

References

[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-242, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09188.1.
PIR	S56202.
RefSeq	NP_116602.1.
3D structure databases
SMR	P43550. Positions 11-345.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6539N.
IntAct	P43550. 7 interactions.
STRING	P43550.
Genome annotation databases
Ensembl	YFL053W; YFL053W; YFL053W; Saccharomyces cerevisiae. [Genome view]
GeneID	850491.
KEGG	sce:YFL053W.
NMPDR	fig|4932.3.peg.2225.
Organism-specific databases
CYGD	YFL053w.
SGD	S000001841. DAK2.
Phylogenomic databases
eggNOG	fuNOG05468.
HOGENOM	HBG473357.
OMA	CILEASI.
OrthoDB	EOG9P2RJV.
PhylomeDB	P43550.
Enzyme and pathway databases
BRENDA	2.7.1.29. 250.
Gene expression databases
ArrayExpress	P43550.
Genevestigator	P43550.
GermOnline	YFL053W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004006. Dak1. IPR004007. Dak2. IPR012734. DhaK_ATP. [Graphical view]
Pfam	PF02733. Dak1. 1 hit. PF02734. Dak2. 1 hit. [Graphical view]
TIGRFAMs	TIGR02361. dak_ATP. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	966167.

Entry information

Entry name

DAK2_YEAST

Accession

Primary (citable) accession number: P43550

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)