Reviewed, UniProtKB/Swiss-Prot P43549 (YFF4_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized membrane protein YFL054C

Gene names

Ordered Locus Names:

YFL054C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transporter activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-22863,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Uncharacterized membrane protein YFL054C		PRO_0000064099
Regions
	Topological domain	1 – 350	350	Cytoplasmic Potential
	Transmembrane	351 – 371	21	Potential
	Topological domain	372 – 383	12	Extracellular Potential
	Transmembrane	384 – 404	21	Potential
	Topological domain	405 – 427	23	Cytoplasmic Potential
	Transmembrane	428 – 448	21	Potential
	Topological domain	449 – 481	33	Extracellular Potential
	Transmembrane	482 – 502	21	Potential
	Topological domain	503 – 509	7	Cytoplasmic Potential
	Transmembrane	510 – 530	21	Potential
	Topological domain	531 – 569	39	Extracellular Potential
	Transmembrane	570 – 590	21	Potential
	Topological domain	591 – 646	56	Cytoplasmic Potential
	Motif	408 – 410	3	NPA 1
	Motif	538 – 540	3	NPA 2
Amino acid modifications
	Modified residue	107	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P43549-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C6A4BC452168C1F9	FASTA	646	70,507
	10 20 30 40 50 60 MSYESGRSSS SSESTRPPTL KEEPNGKIAW EESVKKSREN NENDSTLLRR KLGETRKAIE 70 80 90 100 110 120 TGGSSRNKLS ALTPLKKVVD ERKDSVQPQV PSMGFTYSLP NLKTLNSFSD AEQARIMQDY 130 140 150 160 170 180 LSRGVNQGNS NNYVDPLYRQ LNPTMGSSRN RPVWSLNQPL PHVLDRGLAA KMIQKNMDAR 190 200 210 220 230 240 SRASSRRGST DISRGGSTTS VKDWKRLLRG AAPGKKLGDI EAQTQRDNTV GADVKPTKLE 250 260 270 280 290 300 PENPQKPSNT HIENVSRKKK RTSHNVNFSL GDESYASSIA DAESRKLKNM QTLDGSTPVY 310 320 330 340 350 360 TKLPEELIEE ENKSTSALDG NEIGASEDED ADIMTFPNFW AKIRYHMREP FAEFLGTLVL 370 380 390 400 410 420 VIFGVGGNLQ ATVTKGSGGS YESLSFAWGF GCMLGVYVAG GISGGHINPA VTISMAIFRK 430 440 450 460 470 480 FPWKKVPVYI VAQIIGAYFG GAMAYGYFWS SITEFEGGPH IRTTATGACL FTDPKSYVTW 490 500 510 520 530 540 RNAFFDEFIG ASILVGCLMA LLDDSNAPPG NGMTALIIGF LVAAIGMALG YQTSFTINPA 550 560 570 580 590 600 RDLGPRIFAS MIGYGPHAFH LTHWWWTWGA WGGPIAGGIA GALIYDIFIF TGCESPVNYP 610 620 630 640 DNGYIENRVG KLLHAEFHQN DGTVSDESGV NSNSNTGSKK SVPTSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09187.1. AY692603 Genomic DNA. Translation: AAT92622.1.
PIR	S56201.
RefSeq	NP_116601.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4535N.
IntAct	P43549. 3 interactions.
STRING	P43549.
Protein family/group databases
TCDB	1.A.8.7.1. major intrinsic protein (MIP) family.
Genome annotation databases
Ensembl	YFL054C; YFL054C; YFL054C; Saccharomyces cerevisiae. [Genome view]
GeneID	850490.
KEGG	sce:YFL054C.
NMPDR	fig|4932.3.peg.2224.
Organism-specific databases
CYGD	YFL054c.
SGD	S000001840. YFL054C.
Phylogenomic databases
HOGENOM	P43549.
OMA	SITEFEG
OrthoDB	EOG9FR283
Gene expression databases
ArrayExpress	P43549.
Genevestigator	P43549.
GermOnline	YFL054C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012269. Aquaporin. IPR000425. MIP. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	966164.

Entry information

Entry name

YFF4_YEAST

Accession

Primary (citable) accession number: P43549

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)