Reviewed, UniProtKB/Swiss-Prot P43544 (SNO3_YEAST)

Names and origin

Protein names

Recommended name:
    Probable glutamine amidotransferase SNO3
    EC=2.6.-.-

Gene names

Name:	SNO3
Ordered Locus Names:	YFL060C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be required for the production of pyridoxine (vitamin B6). Ref.2
Miscellaneous	Present with 195 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the glutamine amidotransferase pdxT/SNO family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thiamin biosynthetic process Ref.2 Inferred from mutant phenotype. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SNZ1	Q03148	2	EBI-22871,EBI-17618
SNZ2	P53824	2	EBI-22871,EBI-17623

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Probable glutamine amidotransferase SNO3		PRO_0000135621
Sites
	Active site	91	1	Nucleophile By similarity
	Active site	197	1	By similarity
	Active site	199	1	By similarity
	Binding site	120	1	Glutamine Potential

Sequences

	Sequence		Length	Mass (Da)
	P43544-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C7A0EFA9826FB77C	FASTA	222	25,132
	10 20 30 40 50 60 MTVVIGVLAL QGAFIEHVRH VEKCIVENRD FYEKKLSVMT VKDKNQLAQC DALIIPGGES 70 80 90 100 110 120 TAMSLIAERT GFYDDLYAFV HNPSKVTWGT CAGLIYISQQ LSNEAKLVKT LNLLKVKVKR 130 140 150 160 170 180 NAFGRQAQSS TRICDFSNFI PHCNDFPATF IRAPVIEEVL DPEHVQVLYK LDGKDNGGQE 190 200 210 220 LIVAAKQKNN ILATSFHPEL AENDIRFHDW FIREFVLKNY SK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6." Rodriguez-Navarro S., Llorente B., Rodriguez-Manzaneque M.T., Ramne A., Uber G., Marchesan D., Dujon B., Herrero E., Sunnerhagen P., Perez-Ortin J.E. Yeast 19:1261-1276(2002) [PubMed: 12271461] [Abstract] Cited for: FUNCTION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09181.1.
PIR	S56195.
RefSeq	NP_116595.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1645N.
IntAct	P43544. 6 interactions.
STRING	P43544.
Genome annotation databases
Ensembl	YFL060C; YFL060C; YFL060C; Saccharomyces cerevisiae. [Genome view]
GeneID	850484.
KEGG	sce:YFL060C.
Organism-specific databases
CYGD	YFL060c.
SGD	S000001834. SNO3.
Phylogenomic databases
HOGENOM	P43544.
OMA	YDFMEPL
OrthoDB	EOG9322FF
Gene expression databases
Genevestigator	P43544.
GermOnline	YFL060C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002161. SNO. [Graphical view]
Pfam	PF01174. SNO. 1 hit. [Graphical view]
PIRSF	PIRSF005639. Glut_amidoT_SNO. 1 hit.
PROSITE	PS01236. PDXT_SNO_1. 1 hit. PS51130. PDXT_SNO_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	966146.

Entry information

Entry name

SNO3_YEAST

Accession

Primary (citable) accession number: P43544

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)