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Protein

Probable pyridoxal 5'-phosphate synthase subunit SNO3

Gene

SNO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of a SNZ isoform.By similarity1 Publication

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.By similarity
L-glutamine + H2O = L-glutamate + NH3.By similarity

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91NucleophileBy similarity1
Binding sitei120L-glutamineBy similarity1
Active sitei197Charge relay systemBy similarity1
Active sitei199Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • pyridoxal phosphate biosynthetic process Source: GO_Central
  • pyridoxine metabolic process Source: SGD
  • thiamine biosynthetic process Source: SGD

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:G3O-30407-MONOMER
UniPathwayiUPA00245

Protein family/group databases

MEROPSiC26.A32

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pyridoxal 5'-phosphate synthase subunit SNO3 (EC:4.3.3.6)
Alternative name(s):
PDX2 homolog 3
Short name:
Pdx2.3
Pyridoxal 5'-phosphate synthase glutaminase subunit (EC:3.5.1.2)
Gene namesi
Name:SNO3
Ordered Locus Names:YFL060C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL060C
SGDiS000001834 SNO3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001356211 – 222Probable pyridoxal 5'-phosphate synthase subunit SNO3Add BLAST222

Proteomic databases

MaxQBiP43544
PaxDbiP43544
PRIDEiP43544

Interactioni

Protein-protein interaction databases

BioGridi31087, 9 interactors
DIPiDIP-1645N
IntActiP43544, 5 interactors
MINTiP43544
STRINGi4932.YFL060C

Structurei

3D structure databases

ProteinModelPortaliP43544
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 60L-glutamine bindingBy similarity3
Regioni151 – 152L-glutamine bindingBy similarity2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

GeneTreeiENSGT00390000011516
HOGENOMiHOG000039949
InParanoidiP43544
KOiK08681
OrthoDBiEOG092C4BRK

Family and domain databases

CDDicd01749 GATase1_PB, 1 hit
Gene3Di3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR002161 PdxT/SNO
IPR021196 PdxT/SNO_CS
PANTHERiPTHR31559 PTHR31559, 1 hit
PfamiView protein in Pfam
PF01174 SNO, 1 hit
PIRSFiPIRSF005639 Glut_amidoT_SNO, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR03800 PLP_synth_Pdx2, 1 hit
PROSITEiView protein in PROSITE
PS01236 PDXT_SNO_1, 1 hit
PS51130 PDXT_SNO_2, 1 hit

Sequencei

Sequence statusi: Complete.

P43544-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVVIGVLAL QGAFIEHVRH VEKCIVENRD FYEKKLSVMT VKDKNQLAQC
60 70 80 90 100
DALIIPGGES TAMSLIAERT GFYDDLYAFV HNPSKVTWGT CAGLIYISQQ
110 120 130 140 150
LSNEAKLVKT LNLLKVKVKR NAFGRQAQSS TRICDFSNFI PHCNDFPATF
160 170 180 190 200
IRAPVIEEVL DPEHVQVLYK LDGKDNGGQE LIVAAKQKNN ILATSFHPEL
210 220
AENDIRFHDW FIREFVLKNY SK
Length:222
Mass (Da):25,132
Last modified:November 1, 1995 - v1
Checksum:iC7A0EFA9826FB77C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA Translation: BAA09181.1
BK006940 Genomic DNA Translation: DAA12380.1
PIRiS56195
RefSeqiNP_116595.1, NM_001179907.1

Genome annotation databases

EnsemblFungiiBAA09181; BAA09181; BAA09181
YFL060C; YFL060C; YFL060C
GeneIDi850484
KEGGisce:YFL060C

Similar proteinsi

Entry informationi

Entry nameiSNO3_YEAST
AccessioniPrimary (citable) accession number: P43544
Secondary accession number(s): D6VTH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

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