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Protein

Probable pyridoxal 5'-phosphate synthase subunit SNO3

Gene

SNO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of a SNZ isoform.By similarity1 Publication

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.By similarity
L-glutamine + H2O = L-glutamate + NH3.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911NucleophileBy similarity
Binding sitei120 – 1201L-glutamineBy similarity
Active sitei197 – 1971Charge relay systemBy similarity
Active sitei199 – 1991Charge relay systemBy similarity

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-EC
  2. pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity Source: UniProtKB-EC
  3. transferase activity Source: UniProtKB-KW

GO - Biological processi

  1. glutamine catabolic process Source: InterPro
  2. pyridoxal phosphate biosynthetic process Source: InterPro
  3. pyridoxine biosynthetic process Source: UniProtKB-KW
  4. pyridoxine metabolic process Source: SGD
  5. thiamine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:G3O-30407-MONOMER.
UniPathwayiUPA00245.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pyridoxal 5'-phosphate synthase subunit SNO3 (EC:4.3.3.6)
Alternative name(s):
PDX2 homolog 3
Short name:
Pdx2.3
Pyridoxal 5'-phosphate synthase glutaminase subunit (EC:3.5.1.2)
Gene namesi
Name:SNO3
Ordered Locus Names:YFL060C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL060c.
SGDiS000001834. SNO3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Probable pyridoxal 5'-phosphate synthase subunit SNO3PRO_0000135621Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP43544.

Interactioni

Protein-protein interaction databases

BioGridi31087. 5 interactions.
DIPiDIP-1645N.
IntActiP43544. 5 interactions.
MINTiMINT-386883.
STRINGi4932.YFL060C.

Structurei

3D structure databases

ProteinModelPortaliP43544.
SMRiP43544. Positions 3-203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 603L-glutamine bindingBy similarity
Regioni151 – 1522L-glutamine bindingBy similarity

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

GeneTreeiENSGT00390000011516.
HOGENOMiHOG000039949.
InParanoidiP43544.
KOiK08681.
OMAiPGAEDII.
OrthoDBiEOG7BW0W3.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR021196. PdxT/SNO_CS.
IPR002161. PDXT_SNO_fam.
[Graphical view]
PfamiPF01174. SNO. 1 hit.
[Graphical view]
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiPS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43544-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVVIGVLAL QGAFIEHVRH VEKCIVENRD FYEKKLSVMT VKDKNQLAQC
60 70 80 90 100
DALIIPGGES TAMSLIAERT GFYDDLYAFV HNPSKVTWGT CAGLIYISQQ
110 120 130 140 150
LSNEAKLVKT LNLLKVKVKR NAFGRQAQSS TRICDFSNFI PHCNDFPATF
160 170 180 190 200
IRAPVIEEVL DPEHVQVLYK LDGKDNGGQE LIVAAKQKNN ILATSFHPEL
210 220
AENDIRFHDW FIREFVLKNY SK
Length:222
Mass (Da):25,132
Last modified:November 1, 1995 - v1
Checksum:iC7A0EFA9826FB77C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09181.1.
BK006940 Genomic DNA. Translation: DAA12380.1.
PIRiS56195.
RefSeqiNP_116595.1. NM_001179907.1.

Genome annotation databases

EnsemblFungiiYFL060C; YFL060C; YFL060C.
GeneIDi850484.
KEGGisce:YFL060C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09181.1.
BK006940 Genomic DNA. Translation: DAA12380.1.
PIRiS56195.
RefSeqiNP_116595.1. NM_001179907.1.

3D structure databases

ProteinModelPortaliP43544.
SMRiP43544. Positions 3-203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31087. 5 interactions.
DIPiDIP-1645N.
IntActiP43544. 5 interactions.
MINTiMINT-386883.
STRINGi4932.YFL060C.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYFL060C; YFL060C; YFL060C.
GeneIDi850484.
KEGGisce:YFL060C.

Organism-specific databases

CYGDiYFL060c.
SGDiS000001834. SNO3.

Phylogenomic databases

GeneTreeiENSGT00390000011516.
HOGENOMiHOG000039949.
InParanoidiP43544.
KOiK08681.
OMAiPGAEDII.
OrthoDBiEOG7BW0W3.

Enzyme and pathway databases

UniPathwayiUPA00245.
BioCyciYEAST:G3O-30407-MONOMER.

Miscellaneous databases

NextBioi966146.

Gene expression databases

GenevestigatoriP43544.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR021196. PdxT/SNO_CS.
IPR002161. PDXT_SNO_fam.
[Graphical view]
PfamiPF01174. SNO. 1 hit.
[Graphical view]
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiPS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: FUNCTION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSNO3_YEAST
AccessioniPrimary (citable) accession number: P43544
Secondary accession number(s): D6VTH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 4, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.