P43544 (SNO3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamine amidotransferase SNO3
EC=2.6.-.-

Gene names

Name:	SNO3
Ordered Locus Names:	YFL060C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be required for the production of pyridoxine (vitamin B6). Ref.3
Miscellaneous	Present with 195 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the glutamine amidotransferase PdxT/SNO family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine metabolic process Inferred from direct assay PubMed 9791124. Source: SGD thiamine biosynthetic process Inferred from mutant phenotype Ref.3. Source: SGD
Molecular_function	transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Probable glutamine amidotransferase SNO3		PRO_0000135621
Sites
	Active site	91	1	Nucleophile By similarity
	Active site	197	1	By similarity
	Active site	199	1	By similarity
	Binding site	120	1	Glutamine Potential

Sequences

	Sequence		Length	Mass (Da)
	P43544 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C7A0EFA9826FB77C	FASTA	222	25,132
	10 20 30 40 50 60 MTVVIGVLAL QGAFIEHVRH VEKCIVENRD FYEKKLSVMT VKDKNQLAQC DALIIPGGES 70 80 90 100 110 120 TAMSLIAERT GFYDDLYAFV HNPSKVTWGT CAGLIYISQQ LSNEAKLVKT LNLLKVKVKR 130 140 150 160 170 180 NAFGRQAQSS TRICDFSNFI PHCNDFPATF IRAPVIEEVL DPEHVQVLYK LDGKDNGGQE 190 200 210 220 LIVAAKQKNN ILATSFHPEL AENDIRFHDW FIREFVLKNY SK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6." Rodriguez-Navarro S., Llorente B., Rodriguez-Manzaneque M.T., Ramne A., Uber G., Marchesan D., Dujon B., Herrero E., Sunnerhagen P., Perez-Ortin J.E. Yeast 19:1261-1276(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09181.1. BK006940 Genomic DNA. Translation: DAA12380.1.
PIR	S56195.
RefSeq	NP_116595.1. NM_001179907.1.
3D structure databases
ProteinModelPortal	P43544.
SMR	P43544. Positions 3-203.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1645N.
IntAct	P43544. 5 interactions.
MINT	MINT-386883.
STRING	4932.YFL060C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL060C; YFL060C; YFL060C.
GeneID	850484.
KEGG	sce:YFL060C.
Organism-specific databases
CYGD	YFL060c.
SGD	S000001834. SNO3.
Phylogenomic databases
GeneTree	ENSGT00390000011516.
HOGENOM	HOG000039949.
KO	K08681.
OMA	EEPARRC.
OrthoDB	EOG4R2742.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30407-MONOMER.
Gene expression databases
Genevestigator	P43544.
GermOnline	YFL060C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002161. Glutamine_amidoTferase_suPdxT. IPR021196. PdxT/SNO_CS. [Graphical view]
Pfam	PF01174. SNO. 1 hit. [Graphical view]
PIRSF	PIRSF005639. Glut_amidoT_SNO. 1 hit.
TIGRFAMs	TIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITE	PS01236. PDXT_SNO_1. 1 hit. PS51130. PDXT_SNO_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966146.

Entry information

Entry name

SNO3_YEAST

Accession

Primary (citable) accession number: P43544
Secondary accession number(s): D6VTH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program