P43542 (COS4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein COS4

Gene names

Name:	COS4
Ordered Locus Names:	YFL062W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the DUP/COS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	fungal-type vacuole Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AGP1	P25376	1	EBI-2050225,EBI-2357
CHO1	P08456	1	EBI-2050225,EBI-14055
ERG11	P10614	1	EBI-2050225,EBI-5127
ERV25	P54837	1	EBI-2050225,EBI-6642
GAP1	P19145	1	EBI-2050225,EBI-7314
ICE2	P40499	1	EBI-2050225,EBI-25109
OST1	P41543	1	EBI-2050225,EBI-12651
PHO84	P25297	1	EBI-2050225,EBI-13320
PHO88	P38264	1	EBI-2050225,EBI-13350
PMP2	P40975	1	EBI-2050225,EBI-2043041
SAC1	P32368	1	EBI-2050225,EBI-16210
SPF1	P39986	1	EBI-2050225,EBI-3128
VCX1	Q99385	1	EBI-2050225,EBI-37444
YCR043C	P25361	1	EBI-2050225,EBI-21909
YHR080C	P38800	1	EBI-2050225,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Protein COS4		PRO_0000207515
Regions
	Transmembrane	43 – 63	21	Helical; Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Transmembrane	233 – 253	21	Helical; Potential
	Transmembrane	255 – 275	21	Helical; Potential
Amino acid modifications
	Cross-link	205		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.3 Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P43542 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6822561BC9890275	FASTA	379	45,312
	10 20 30 40 50 60 MKENELKNEK SVDVLSFKQL ESQKIVLPQD LFRSSFTWFC YEIYKSLAFR IWMLLWLPLS 70 80 90 100 110 120 VWWKLSNNWI YPLMVSLLVL FWGPVFVLVI FRLSRKRSLS KQLTQFCKEI TKSTPSSDPH 130 140 150 160 170 180 DWEVVAANLN SYLYENKAWN IRYFFFNAMG CQEAFRTTLL EPFSLKKDEA AKVKSFKDSV 190 200 210 220 230 240 PYIEEALGVY FREVEKQWKL FNSEKSWSPV GLEDAKLPKE AYRFKLTWFL KRISNIFMLI 250 260 270 280 290 300 PFLNFLCCIY VSRGMCLLLR TLYLGWILFM LVQGFQNIRV LIMSMEHKMQ FLSTIINEQE 310 320 330 340 350 360 SGANGWDEIA RKMNRYLFEK KVWKNEEFFF DGIDCEWFFS HFFYRVLSAK KSMRALSLNV 370 ELWPYIKEAQ LSCSEESLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-205, MASS SPECTROMETRY. Strain: SUB592.
[4]	"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-205, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09179.1. BK006940 Genomic DNA. Translation: DAA12378.1.
PIR	S56193.
RefSeq	NP_116593.1. NM_001179905.1.
3D structure databases
ProteinModelPortal	P43542.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7524N.
IntAct	P43542. 15 interactions.
MINT	MINT-1355277.
STRING	P43542.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL062W; YFL062W; YFL062W.
GeneID	850482.
KEGG	sce:YFL062W.
NMPDR	fig|4932.3.peg.2216.
Organism-specific databases
CYGD	YFL062w.
SGD	S000001832. COS4.
Phylogenomic databases
GeneTree	EFGT00050000008557.
HOGENOM	HBG204451.
Gene expression databases
Genevestigator	P43542.
GermOnline	YFL062W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001142. DUP. [Graphical view]
Pfam	PF00674. DUP. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	966140.

Entry information

Entry name

COS4_YEAST

Accession

Primary (citable) accession number: P43542
Secondary accession number(s): D6VTG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program