P43535 (GCN20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein GCN20

Gene names

Name:	GCN20
Ordered Locus Names:	YFR009W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	752 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive effector of the eIF-2-alpha kinase activity of GCN2.
Subunit structure	Component of a heteromeric complex that includes GCN1 and GCN20.
Miscellaneous	Present with 14600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. Contains 2 ABC transporter domains.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of translational elongation Inferred from physical interaction PubMed 9234705. Source: SGD
Cellular_component	cytosolic ribosome Inferred from direct assay PubMed 9234705. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from sequence or structural similarity Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GCN1	P33892	4	EBI-7423,EBI-7442

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 752	752	Protein GCN20		PRO_0000093461
Regions
	Domain	199 – 464	266	ABC transporter 1
	Domain	532 – 748	217	ABC transporter 2
	Nucleotide binding	232 – 239	8	ATP 1 Potential
	Nucleotide binding	565 – 572	8	ATP 2 Potential
Amino acid modifications
	Modified residue	95	1	Phosphoserine Ref.6
	Modified residue	742	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P43535 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 486FED10305A572E	FASTA	752	85,027
	10 20 30 40 50 60 MASIGSQVRK AASSIDPIVT DYAVGYFNHL SGITFDAVQS KQVDLSTEVQ FVSDLLIDAG 70 80 90 100 110 120 ASKAKVKELS ESILKQLTTQ LKENEAKLEL TGDTSKRLLD INVLKSHNSK SDINVSLSML 130 140 150 160 170 180 GVNGDIEHTG RKMETRVDLK KLAKAEQKIA KKVAKRNNKF VKYEASKLIN DQKEEDYDSF 190 200 210 220 230 240 FLQINPLEFG SSAGKSKDIH IDTFDLYVGD GQRILSNAQL TLSFGHRYGL VGQNGIGKST 250 260 270 280 290 300 LLRALSRREL NVPKHVSILH VEQELRGDDT KALQSVLDAD VWRKQLLSEE AKINERLKEM 310 320 330 340 350 360 DVLRQEFEED SLEVKKLDNE REDLDNHLIQ ISDKLVDMES DKAEARAASI LYGLGFSTEA 370 380 390 400 410 420 QQQPTNSFSG GWRMRLSLAR ALFCQPDLLL LDEPSNMLDV PSIAYLAEYL KTYPNTVLTV 430 440 450 460 470 480 SHDRAFLNEV ATDIIYQHNE RLDYYRGQDF DTFYTTKEER RKNAQREYDN QMVYRKHLQE 490 500 510 520 530 540 FIDKYRYNAA KSQEAQSRIK KLEKLPVLEP PEQDKTIDFK FPECDKLSPP IIQLQDVSFG 550 560 570 580 590 600 YDENNLLLKD VNLDVQMDSR IALVGANGCG KTTLLKIMME QLRPLKGFVS RNPRLRIGYF 610 620 630 640 650 660 TQHHVDSMDL TTSAVDWMSK SFPGKTDEEY RRHLGSFGIT GTLGLQKMQL LSGGQKSRVA 670 680 690 700 710 720 FAALCLNNPH ILVLDEPSNH LDTTGLDALV EALKNFNGGV LMVSHDISVI DSVCKEIWVS 730 740 750 EQGTVKRFEG TIYDYRDYIL QSADAAGVVK KH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells." Vazquez de Aldana C.R., Marton M.J., Hinnebusch A.G. EMBO J. 14:3184-3199(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95 AND SER-742, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19971 Genomic DNA. Translation: AAA75444.1. D50617 Genomic DNA. Translation: BAA09248.1. AY723804 Genomic DNA. Translation: AAU09721.1. BK006940 Genomic DNA. Translation: DAA12449.1.
PIR	S56146.
RefSeq	NP_116664.1. NM_001179974.1.
3D structure databases
ProteinModelPortal	P43535.
SMR	P43535. Positions 211-725.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2345N.
IntAct	P43535. 51 interactions.
MINT	MINT-634628.
STRING	4932.YFR009W.
Proteomic databases
PaxDb	P43535.
PeptideAtlas	P43535.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR009W; YFR009W; YFR009W.
GeneID	850561.
KEGG	sce:YFR009W.
Organism-specific databases
CYGD	YFR009w.
SGD	S000001905. GCN20.
Phylogenomic databases
eggNOG	COG0488.
GeneTree	ENSGT00630000089843.
HOGENOM	HOG000271637.
KO	K06158.
OMA	AEYLKTY.
OrthoDB	EOG44N215.
Gene expression databases
Genevestigator	P43535.
GermOnline	YFR009W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. [Graphical view]
Pfam	PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	966358.

Entry information

Entry name

GCN20_YEAST

Accession

Primary (citable) accession number: P43535
Secondary accession number(s): D6VTN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program