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P43535 (GCN20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein GCN20
Gene names
Name:GCN20
Ordered Locus Names:YFR009W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length752 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positive effector of the eIF-2-alpha kinase activity of GCN2.

Subunit structure

Component of a heteromeric complex that includes GCN1 and GCN20.

Miscellaneous

Present with 14600 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.

Contains 2 ABC transporter domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GCN1P338924EBI-7423,EBI-7442

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 752751Protein GCN20
PRO_0000093461

Regions

Domain199 – 464266ABC transporter 1
Domain532 – 748217ABC transporter 2
Nucleotide binding232 – 2398ATP 1 Potential
Nucleotide binding565 – 5728ATP 2 Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P43535 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 486FED10305A572E

FASTA75285,027
        10         20         30         40         50         60 
MASIGSQVRK AASSIDPIVT DYAVGYFNHL SGITFDAVQS KQVDLSTEVQ FVSDLLIDAG 

        70         80         90        100        110        120 
ASKAKVKELS ESILKQLTTQ LKENEAKLEL TGDTSKRLLD INVLKSHNSK SDINVSLSML 

       130        140        150        160        170        180 
GVNGDIEHTG RKMETRVDLK KLAKAEQKIA KKVAKRNNKF VKYEASKLIN DQKEEDYDSF 

       190        200        210        220        230        240 
FLQINPLEFG SSAGKSKDIH IDTFDLYVGD GQRILSNAQL TLSFGHRYGL VGQNGIGKST 

       250        260        270        280        290        300 
LLRALSRREL NVPKHVSILH VEQELRGDDT KALQSVLDAD VWRKQLLSEE AKINERLKEM 

       310        320        330        340        350        360 
DVLRQEFEED SLEVKKLDNE REDLDNHLIQ ISDKLVDMES DKAEARAASI LYGLGFSTEA 

       370        380        390        400        410        420 
QQQPTNSFSG GWRMRLSLAR ALFCQPDLLL LDEPSNMLDV PSIAYLAEYL KTYPNTVLTV 

       430        440        450        460        470        480 
SHDRAFLNEV ATDIIYQHNE RLDYYRGQDF DTFYTTKEER RKNAQREYDN QMVYRKHLQE 

       490        500        510        520        530        540 
FIDKYRYNAA KSQEAQSRIK KLEKLPVLEP PEQDKTIDFK FPECDKLSPP IIQLQDVSFG 

       550        560        570        580        590        600 
YDENNLLLKD VNLDVQMDSR IALVGANGCG KTTLLKIMME QLRPLKGFVS RNPRLRIGYF 

       610        620        630        640        650        660 
TQHHVDSMDL TTSAVDWMSK SFPGKTDEEY RRHLGSFGIT GTLGLQKMQL LSGGQKSRVA 

       670        680        690        700        710        720 
FAALCLNNPH ILVLDEPSNH LDTTGLDALV EALKNFNGGV LMVSHDISVI DSVCKEIWVS 

       730        740        750 
EQGTVKRFEG TIYDYRDYIL QSADAAGVVK KH 

« Hide

References

« Hide 'large scale' references
[1]"GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells."
Vazquez de Aldana C.R., Marton M.J., Hinnebusch A.G.
EMBO J. 14:3184-3199(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U19971 Genomic DNA. Translation: AAA75444.1.
D50617 Genomic DNA. Translation: BAA09248.1.
AY723804 Genomic DNA. Translation: AAU09721.1.
BK006940 Genomic DNA. Translation: DAA12449.1.
PIRS56146.
RefSeqNP_116664.1. NM_001179974.1.

3D structure databases

ProteinModelPortalP43535.
SMRP43535. Positions 211-738.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31159. 162 interactions.
DIPDIP-2345N.
IntActP43535. 53 interactions.
MINTMINT-634628.
STRING4932.YFR009W.

Proteomic databases

MaxQBP43535.
PaxDbP43535.
PeptideAtlasP43535.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR009W; YFR009W; YFR009W.
GeneID850561.
KEGGsce:YFR009W.

Organism-specific databases

CYGDYFR009w.
SGDS000001905. GCN20.

Phylogenomic databases

eggNOGCOG0488.
GeneTreeENSGT00630000089843.
HOGENOMHOG000271637.
KOK06158.
OMAVAKRNNK.
OrthoDBEOG718KNB.

Enzyme and pathway databases

BioCycYEAST:G3O-30462-MONOMER.

Gene expression databases

GenevestigatorP43535.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio966358.
PROP43535.

Entry information

Entry nameGCN20_YEAST
AccessionPrimary (citable) accession number: P43535
Secondary accession number(s): D6VTN9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families