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P43535

- GCN20_YEAST

UniProt

P43535 - GCN20_YEAST

Protein

Protein GCN20

Gene

GCN20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Positive effector of the eIF-2-alpha kinase activity of GCN2.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi232 – 2398ATP 1PROSITE-ProRule annotation
    Nucleotide bindingi565 – 5728ATP 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. regulation of translational elongation Source: SGD

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30462-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein GCN20
    Gene namesi
    Name:GCN20
    Ordered Locus Names:YFR009W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR009w.
    SGDiS000001905. GCN20.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. cytosolic ribosome Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 752751Protein GCN20PRO_0000093461Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP43535.
    PaxDbiP43535.
    PeptideAtlasiP43535.

    Expressioni

    Gene expression databases

    GenevestigatoriP43535.

    Interactioni

    Subunit structurei

    Component of a heteromeric complex that includes GCN1 and GCN20.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GCN1P338924EBI-7423,EBI-7442

    Protein-protein interaction databases

    BioGridi31159. 164 interactions.
    DIPiDIP-2345N.
    IntActiP43535. 53 interactions.
    MINTiMINT-634628.
    STRINGi4932.YFR009W.

    Structurei

    3D structure databases

    ProteinModelPortaliP43535.
    SMRiP43535. Positions 211-738.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini199 – 464266ABC transporter 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini532 – 748217ABC transporter 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ABC transporter domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0488.
    GeneTreeiENSGT00630000089843.
    HOGENOMiHOG000271637.
    KOiK06158.
    OMAiVAKRNNK.
    OrthoDBiEOG718KNB.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00005. ABC_tran. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43535-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASIGSQVRK AASSIDPIVT DYAVGYFNHL SGITFDAVQS KQVDLSTEVQ    50
    FVSDLLIDAG ASKAKVKELS ESILKQLTTQ LKENEAKLEL TGDTSKRLLD 100
    INVLKSHNSK SDINVSLSML GVNGDIEHTG RKMETRVDLK KLAKAEQKIA 150
    KKVAKRNNKF VKYEASKLIN DQKEEDYDSF FLQINPLEFG SSAGKSKDIH 200
    IDTFDLYVGD GQRILSNAQL TLSFGHRYGL VGQNGIGKST LLRALSRREL 250
    NVPKHVSILH VEQELRGDDT KALQSVLDAD VWRKQLLSEE AKINERLKEM 300
    DVLRQEFEED SLEVKKLDNE REDLDNHLIQ ISDKLVDMES DKAEARAASI 350
    LYGLGFSTEA QQQPTNSFSG GWRMRLSLAR ALFCQPDLLL LDEPSNMLDV 400
    PSIAYLAEYL KTYPNTVLTV SHDRAFLNEV ATDIIYQHNE RLDYYRGQDF 450
    DTFYTTKEER RKNAQREYDN QMVYRKHLQE FIDKYRYNAA KSQEAQSRIK 500
    KLEKLPVLEP PEQDKTIDFK FPECDKLSPP IIQLQDVSFG YDENNLLLKD 550
    VNLDVQMDSR IALVGANGCG KTTLLKIMME QLRPLKGFVS RNPRLRIGYF 600
    TQHHVDSMDL TTSAVDWMSK SFPGKTDEEY RRHLGSFGIT GTLGLQKMQL 650
    LSGGQKSRVA FAALCLNNPH ILVLDEPSNH LDTTGLDALV EALKNFNGGV 700
    LMVSHDISVI DSVCKEIWVS EQGTVKRFEG TIYDYRDYIL QSADAAGVVK 750
    KH 752
    Length:752
    Mass (Da):85,027
    Last modified:November 1, 1995 - v1
    Checksum:i486FED10305A572E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19971 Genomic DNA. Translation: AAA75444.1.
    D50617 Genomic DNA. Translation: BAA09248.1.
    AY723804 Genomic DNA. Translation: AAU09721.1.
    BK006940 Genomic DNA. Translation: DAA12449.1.
    PIRiS56146.
    RefSeqiNP_116664.1. NM_001179974.1.

    Genome annotation databases

    EnsemblFungiiYFR009W; YFR009W; YFR009W.
    GeneIDi850561.
    KEGGisce:YFR009W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19971 Genomic DNA. Translation: AAA75444.1 .
    D50617 Genomic DNA. Translation: BAA09248.1 .
    AY723804 Genomic DNA. Translation: AAU09721.1 .
    BK006940 Genomic DNA. Translation: DAA12449.1 .
    PIRi S56146.
    RefSeqi NP_116664.1. NM_001179974.1.

    3D structure databases

    ProteinModelPortali P43535.
    SMRi P43535. Positions 211-738.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31159. 164 interactions.
    DIPi DIP-2345N.
    IntActi P43535. 53 interactions.
    MINTi MINT-634628.
    STRINGi 4932.YFR009W.

    Proteomic databases

    MaxQBi P43535.
    PaxDbi P43535.
    PeptideAtlasi P43535.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR009W ; YFR009W ; YFR009W .
    GeneIDi 850561.
    KEGGi sce:YFR009W.

    Organism-specific databases

    CYGDi YFR009w.
    SGDi S000001905. GCN20.

    Phylogenomic databases

    eggNOGi COG0488.
    GeneTreei ENSGT00630000089843.
    HOGENOMi HOG000271637.
    KOi K06158.
    OMAi VAKRNNK.
    OrthoDBi EOG718KNB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30462-MONOMER.

    Miscellaneous databases

    NextBioi 966358.
    PROi P43535.

    Gene expression databases

    Genevestigatori P43535.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00005. ABC_tran. 2 hits.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells."
      Vazquez de Aldana C.R., Marton M.J., Hinnebusch A.G.
      EMBO J. 14:3184-3199(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGCN20_YEAST
    AccessioniPrimary (citable) accession number: P43535
    Secondary accession number(s): D6VTN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 14600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3