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Protein

Protein GCN20

Gene

GCN20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a positive activator of the GCN2 protein kinase activity in response to amino acid starvation (PubMed:7621831). Component of the GCN1-GCN20 complex that forms a complex with GCN2 on translating ribosomes; during this process, GCN20 helps GCN1 to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of GCN2, and hence stimulating GCN2 kinase activity (PubMed:7621831, PubMed:9234705, PubMed:10775272, PubMed:15722345). Participates in gene-specific mRNA translation activation, such as the transcriptional activator GCN4, by promoting the GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/SUI2) on 'Ser-52', and hence allowing GCN4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:15722345).4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi232 – 239ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi565 – 572ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • ribosome binding Source: UniProtKB
  • translation initiation factor binding Source: UniProtKB

GO - Biological processi

  • cellular response to histidine Source: UniProtKB
  • positive regulation of phosphorylation Source: UniProtKB
  • positive regulation of translational initiation in response to starvation Source: UniProtKB
  • regulation of translational elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30462-MONOMER.
ReactomeiR-SCE-382556. ABC-family proteins mediated transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GCN20Curated
Alternative name(s):
General control non-derepressible protein 20Imported
Gene namesi
Name:GCN20Imported
Ordered Locus Names:YFR009W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR009W.
SGDiS000001905. GCN20.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • cytosolic ribosome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000934612 – 752Protein GCN20Add BLAST751

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP43535.
PRIDEiP43535.

PTM databases

iPTMnetiP43535.

Interactioni

Subunit structurei

Interacts (via N-terminus) with GCN1 (via C-terminus); this interaction stimulates GCN2 kinase activity in response to amino acid starvation (PubMed:7621831, PubMed:9234705, PubMed:11101534, PubMed:15722345). The GCN1-GCN20 complex interacts with GCN2 on translating ribosomes in amino acid-starved cells; this association stimulates GCN2 kinase activation by uncharged tRNAs, and hence allowing GCN4 translational activation and derepression of amino acid biosynthetic genes (PubMed:7621831, PubMed:9234705, PubMed:10775272, PubMed:11101534). Associates with ribosomes (PubMed:7621831, PubMed:9234705, PubMed:15722345).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GCN1P338924EBI-7423,EBI-7442

GO - Molecular functioni

  • translation initiation factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi31159. 160 interactors.
DIPiDIP-2345N.
IntActiP43535. 54 interactors.
MINTiMINT-634628.

Structurei

3D structure databases

ProteinModelPortaliP43535.
SMRiP43535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini199 – 464ABC transporter 1PROSITE-ProRule annotationAdd BLAST266
Domaini532 – 748ABC transporter 2PROSITE-ProRule annotationAdd BLAST217

Sequence similaritiesi

Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00630000089843.
HOGENOMiHOG000271637.
InParanoidiP43535.
KOiK06158.
OMAiWKNEEVR.
OrthoDBiEOG092C22OB.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR032781. ABC_tran_Xtn.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF12848. ABC_tran_Xtn. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43535-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIGSQVRK AASSIDPIVT DYAVGYFNHL SGITFDAVQS KQVDLSTEVQ
60 70 80 90 100
FVSDLLIDAG ASKAKVKELS ESILKQLTTQ LKENEAKLEL TGDTSKRLLD
110 120 130 140 150
INVLKSHNSK SDINVSLSML GVNGDIEHTG RKMETRVDLK KLAKAEQKIA
160 170 180 190 200
KKVAKRNNKF VKYEASKLIN DQKEEDYDSF FLQINPLEFG SSAGKSKDIH
210 220 230 240 250
IDTFDLYVGD GQRILSNAQL TLSFGHRYGL VGQNGIGKST LLRALSRREL
260 270 280 290 300
NVPKHVSILH VEQELRGDDT KALQSVLDAD VWRKQLLSEE AKINERLKEM
310 320 330 340 350
DVLRQEFEED SLEVKKLDNE REDLDNHLIQ ISDKLVDMES DKAEARAASI
360 370 380 390 400
LYGLGFSTEA QQQPTNSFSG GWRMRLSLAR ALFCQPDLLL LDEPSNMLDV
410 420 430 440 450
PSIAYLAEYL KTYPNTVLTV SHDRAFLNEV ATDIIYQHNE RLDYYRGQDF
460 470 480 490 500
DTFYTTKEER RKNAQREYDN QMVYRKHLQE FIDKYRYNAA KSQEAQSRIK
510 520 530 540 550
KLEKLPVLEP PEQDKTIDFK FPECDKLSPP IIQLQDVSFG YDENNLLLKD
560 570 580 590 600
VNLDVQMDSR IALVGANGCG KTTLLKIMME QLRPLKGFVS RNPRLRIGYF
610 620 630 640 650
TQHHVDSMDL TTSAVDWMSK SFPGKTDEEY RRHLGSFGIT GTLGLQKMQL
660 670 680 690 700
LSGGQKSRVA FAALCLNNPH ILVLDEPSNH LDTTGLDALV EALKNFNGGV
710 720 730 740 750
LMVSHDISVI DSVCKEIWVS EQGTVKRFEG TIYDYRDYIL QSADAAGVVK

KH
Length:752
Mass (Da):85,027
Last modified:November 1, 1995 - v1
Checksum:i486FED10305A572E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19971 Genomic DNA. Translation: AAA75444.1.
D50617 Genomic DNA. Translation: BAA09248.1.
AY723804 Genomic DNA. Translation: AAU09721.1.
BK006940 Genomic DNA. Translation: DAA12449.1.
PIRiS56146.
RefSeqiNP_116664.1. NM_001179974.1.

Genome annotation databases

EnsemblFungiiBAA09248; BAA09248; BAA09248.
YFR009W; YFR009W; YFR009W.
GeneIDi850561.
KEGGisce:YFR009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19971 Genomic DNA. Translation: AAA75444.1.
D50617 Genomic DNA. Translation: BAA09248.1.
AY723804 Genomic DNA. Translation: AAU09721.1.
BK006940 Genomic DNA. Translation: DAA12449.1.
PIRiS56146.
RefSeqiNP_116664.1. NM_001179974.1.

3D structure databases

ProteinModelPortaliP43535.
SMRiP43535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31159. 160 interactors.
DIPiDIP-2345N.
IntActiP43535. 54 interactors.
MINTiMINT-634628.

PTM databases

iPTMnetiP43535.

Proteomic databases

MaxQBiP43535.
PRIDEiP43535.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09248; BAA09248; BAA09248.
YFR009W; YFR009W; YFR009W.
GeneIDi850561.
KEGGisce:YFR009W.

Organism-specific databases

EuPathDBiFungiDB:YFR009W.
SGDiS000001905. GCN20.

Phylogenomic databases

GeneTreeiENSGT00630000089843.
HOGENOMiHOG000271637.
InParanoidiP43535.
KOiK06158.
OMAiWKNEEVR.
OrthoDBiEOG092C22OB.

Enzyme and pathway databases

BioCyciYEAST:G3O-30462-MONOMER.
ReactomeiR-SCE-382556. ABC-family proteins mediated transport.

Miscellaneous databases

PROiP43535.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR032781. ABC_tran_Xtn.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF12848. ABC_tran_Xtn. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCN20_YEAST
AccessioniPrimary (citable) accession number: P43535
Secondary accession number(s): D6VTN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 14600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.