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P43535

- GCN20_YEAST

UniProt

P43535 - GCN20_YEAST

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Protein
Protein GCN20
Gene
GCN20, YFR009W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Positive effector of the eIF-2-alpha kinase activity of GCN2.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi232 – 2398ATP 1 Reviewed prediction
Nucleotide bindingi565 – 5728ATP 2 Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. regulation of translational elongation Source: SGD
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30462-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GCN20
Gene namesi
Name:GCN20
Ordered Locus Names:YFR009W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFR009w.
SGDiS000001905. GCN20.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. cytosolic ribosome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 752751Protein GCN20
PRO_0000093461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP43535.
PaxDbiP43535.
PeptideAtlasiP43535.

Expressioni

Gene expression databases

GenevestigatoriP43535.

Interactioni

Subunit structurei

Component of a heteromeric complex that includes GCN1 and GCN20.

Binary interactionsi

WithEntry#Exp.IntActNotes
GCN1P338924EBI-7423,EBI-7442

Protein-protein interaction databases

BioGridi31159. 164 interactions.
DIPiDIP-2345N.
IntActiP43535. 53 interactions.
MINTiMINT-634628.
STRINGi4932.YFR009W.

Structurei

3D structure databases

ProteinModelPortaliP43535.
SMRiP43535. Positions 211-738.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini199 – 464266ABC transporter 1
Add
BLAST
Domaini532 – 748217ABC transporter 2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0488.
GeneTreeiENSGT00630000089843.
HOGENOMiHOG000271637.
KOiK06158.
OMAiVAKRNNK.
OrthoDBiEOG718KNB.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43535-1 [UniParc]FASTAAdd to Basket

« Hide

MASIGSQVRK AASSIDPIVT DYAVGYFNHL SGITFDAVQS KQVDLSTEVQ    50
FVSDLLIDAG ASKAKVKELS ESILKQLTTQ LKENEAKLEL TGDTSKRLLD 100
INVLKSHNSK SDINVSLSML GVNGDIEHTG RKMETRVDLK KLAKAEQKIA 150
KKVAKRNNKF VKYEASKLIN DQKEEDYDSF FLQINPLEFG SSAGKSKDIH 200
IDTFDLYVGD GQRILSNAQL TLSFGHRYGL VGQNGIGKST LLRALSRREL 250
NVPKHVSILH VEQELRGDDT KALQSVLDAD VWRKQLLSEE AKINERLKEM 300
DVLRQEFEED SLEVKKLDNE REDLDNHLIQ ISDKLVDMES DKAEARAASI 350
LYGLGFSTEA QQQPTNSFSG GWRMRLSLAR ALFCQPDLLL LDEPSNMLDV 400
PSIAYLAEYL KTYPNTVLTV SHDRAFLNEV ATDIIYQHNE RLDYYRGQDF 450
DTFYTTKEER RKNAQREYDN QMVYRKHLQE FIDKYRYNAA KSQEAQSRIK 500
KLEKLPVLEP PEQDKTIDFK FPECDKLSPP IIQLQDVSFG YDENNLLLKD 550
VNLDVQMDSR IALVGANGCG KTTLLKIMME QLRPLKGFVS RNPRLRIGYF 600
TQHHVDSMDL TTSAVDWMSK SFPGKTDEEY RRHLGSFGIT GTLGLQKMQL 650
LSGGQKSRVA FAALCLNNPH ILVLDEPSNH LDTTGLDALV EALKNFNGGV 700
LMVSHDISVI DSVCKEIWVS EQGTVKRFEG TIYDYRDYIL QSADAAGVVK 750
KH 752
Length:752
Mass (Da):85,027
Last modified:November 1, 1995 - v1
Checksum:i486FED10305A572E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U19971 Genomic DNA. Translation: AAA75444.1.
D50617 Genomic DNA. Translation: BAA09248.1.
AY723804 Genomic DNA. Translation: AAU09721.1.
BK006940 Genomic DNA. Translation: DAA12449.1.
PIRiS56146.
RefSeqiNP_116664.1. NM_001179974.1.

Genome annotation databases

EnsemblFungiiYFR009W; YFR009W; YFR009W.
GeneIDi850561.
KEGGisce:YFR009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U19971 Genomic DNA. Translation: AAA75444.1 .
D50617 Genomic DNA. Translation: BAA09248.1 .
AY723804 Genomic DNA. Translation: AAU09721.1 .
BK006940 Genomic DNA. Translation: DAA12449.1 .
PIRi S56146.
RefSeqi NP_116664.1. NM_001179974.1.

3D structure databases

ProteinModelPortali P43535.
SMRi P43535. Positions 211-738.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31159. 164 interactions.
DIPi DIP-2345N.
IntActi P43535. 53 interactions.
MINTi MINT-634628.
STRINGi 4932.YFR009W.

Proteomic databases

MaxQBi P43535.
PaxDbi P43535.
PeptideAtlasi P43535.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFR009W ; YFR009W ; YFR009W .
GeneIDi 850561.
KEGGi sce:YFR009W.

Organism-specific databases

CYGDi YFR009w.
SGDi S000001905. GCN20.

Phylogenomic databases

eggNOGi COG0488.
GeneTreei ENSGT00630000089843.
HOGENOMi HOG000271637.
KOi K06158.
OMAi VAKRNNK.
OrthoDBi EOG718KNB.

Enzyme and pathway databases

BioCyci YEAST:G3O-30462-MONOMER.

Miscellaneous databases

NextBioi 966358.
PROi P43535.

Gene expression databases

Genevestigatori P43535.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00005. ABC_tran. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells."
    Vazquez de Aldana C.R., Marton M.J., Hinnebusch A.G.
    EMBO J. 14:3184-3199(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGCN20_YEAST
AccessioniPrimary (citable) accession number: P43535
Secondary accession number(s): D6VTN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 14600 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi