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P43534

- THI5_YEAST

UniProt

P43534 - THI5_YEAST

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Protein
Pyrimidine precursor biosynthesis enzyme THI5
Gene
THI5, YFL058W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. thiamine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Thiamine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-3915.
YEAST:MONOMER3O-3915.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrimidine precursor biosynthesis enzyme THI5
Gene namesi
Name:THI5
Ordered Locus Names:YFL058W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFL058w.
SGDiS000001836. THI5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Pyrimidine precursor biosynthesis enzyme THI5
PRO_0000211618Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP43534.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-19221,EBI-16219

Protein-protein interaction databases

BioGridi31089. 2 interactions.
DIPiDIP-3951N.
IntActiP43534. 1 interaction.
MINTiMINT-525063.
STRINGi4932.YFL058W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 94
Beta strandi11 – 133
Helixi16 – 183
Helixi19 – 268
Helixi29 – 324
Beta strandi37 – 448
Helixi45 – 473
Helixi48 – 547
Beta strandi55 – 573
Beta strandi59 – 635
Helixi64 – 718
Turni72 – 743
Beta strandi77 – 848
Beta strandi89 – 946
Turni95 – 984
Helixi103 – 1064
Beta strandi110 – 1134
Helixi117 – 12711
Turni128 – 1303
Beta strandi135 – 1406
Helixi142 – 1443
Helixi145 – 1506
Beta strandi153 – 1608
Turni161 – 1633
Helixi164 – 17411
Helixi179 – 1813
Beta strandi182 – 1865
Helixi196 – 1994
Beta strandi201 – 2066
Helixi207 – 2126
Helixi214 – 23320
Helixi235 – 24511
Helixi247 – 2493
Helixi252 – 26110
Turni262 – 2643
Helixi273 – 28513
Helixi315 – 33016

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H65X-ray2.60A/B1-340[»]
4H67X-ray2.70A/B/C/D/E/F/G/H1-340[»]
4H6DX-ray2.90A/B/C/D/E/F/G/H1-340[»]
ProteinModelPortaliP43534.
SMRiP43534. Positions 4-338.

Family & Domainsi

Sequence similaritiesi

Belongs to the NMT1 family.

Phylogenomic databases

GeneTreeiENSGT00390000017029.
HOGENOMiHOG000160891.
KOiK18278.
OMAiGGYINHE.
OrthoDBiEOG7DRJD1.

Family and domain databases

InterProiIPR027939. NMT1/THI5.
IPR015168. SsuA/THI5.
[Graphical view]
PANTHERiPTHR31528. PTHR31528. 1 hit.
PfamiPF09084. NMT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43534-1 [UniParc]FASTAAdd to Basket

« Hide

MSTDKITFLL NWQPTPYHIP IFLAQTKGYF KEQGLDMAIL EPTNPSDVTE    50
LIGSGKVDMG LKAMIHTLAA KARGFPVTSV ASLLDEPFTG VLYLKGSGIT 100
EDFQSLKGKK IGYVGEFGKI QIDELTKHYG MKPEDYTAVR CGMNVAKYII 150
EGKIDAGIGI ECMQQVELEE YLAKQGRPAS DAKMLRIDKL ACLGCCCFCT 200
VLYICNDEFL KKNPEKVRKF LKAIKKATDY VLADPVKAWK EYIDFKPQLN 250
NDLSYKQYQR CYAYFSSSLY NVHRDWKKVT GYGKRLAILP PDYVSNYTNE 300
YLSWPEPEEV SDPLEAQRLM AIHQEKCRQE GTFKRLALPA 340
Length:340
Mass (Da):38,592
Last modified:November 1, 1995 - v1
Checksum:iD810C5D5A86FA6C0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48220 Genomic DNA. Translation: CAA88253.1.
D50617 Genomic DNA. Translation: BAA09183.1.
BK006940 Genomic DNA. Translation: DAA12382.1.
RefSeqiNP_116597.1. NM_001179909.1.

Genome annotation databases

EnsemblFungiiYFL058W; YFL058W; YFL058W.
GeneIDi850486.
KEGGisce:YFL058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48220 Genomic DNA. Translation: CAA88253.1 .
D50617 Genomic DNA. Translation: BAA09183.1 .
BK006940 Genomic DNA. Translation: DAA12382.1 .
RefSeqi NP_116597.1. NM_001179909.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4H65 X-ray 2.60 A/B 1-340 [» ]
4H67 X-ray 2.70 A/B/C/D/E/F/G/H 1-340 [» ]
4H6D X-ray 2.90 A/B/C/D/E/F/G/H 1-340 [» ]
ProteinModelPortali P43534.
SMRi P43534. Positions 4-338.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31089. 2 interactions.
DIPi DIP-3951N.
IntActi P43534. 1 interaction.
MINTi MINT-525063.
STRINGi 4932.YFL058W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFL058W ; YFL058W ; YFL058W .
GeneIDi 850486.
KEGGi sce:YFL058W.

Organism-specific databases

CYGDi YFL058w.
SGDi S000001836. THI5.

Phylogenomic databases

GeneTreei ENSGT00390000017029.
HOGENOMi HOG000160891.
KOi K18278.
OMAi GGYINHE.
OrthoDBi EOG7DRJD1.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER3O-3915.
YEAST:MONOMER3O-3915.

Miscellaneous databases

NextBioi 966152.

Gene expression databases

Genevestigatori P43534.

Family and domain databases

InterProi IPR027939. NMT1/THI5.
IPR015168. SsuA/THI5.
[Graphical view ]
PANTHERi PTHR31528. PTHR31528. 1 hit.
Pfami PF09084. NMT1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Hather R.J., Praekelt U., Meacock P.A.
    Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine."
    Wightman R., Meacock P.A.
    Microbiology 149:1447-1460(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTHI5_YEAST
AccessioniPrimary (citable) accession number: P43534
Secondary accession number(s): D6VTH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

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