P43534 (THI5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrimidine precursor biosynthesis enzyme THI5

Gene names

Name:	THI5
Ordered Locus Names:	YFL058W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety. Ref.4
Sequence similarities	Belongs to the NMT1 family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	thiamine biosynthetic process Inferred from genetic interaction Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSP5	P39940	2	EBI-19221,EBI-16219

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 340

340

Pyrimidine precursor biosynthesis enzyme THI5

PRO_0000211618

Secondary structure

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340

Helix Strand Turn

Details...

Beta strand

6 – 9

4

Beta strand

11 – 13

3

Helix

16 – 18

3

Helix

19 – 26

8

Helix

29 – 32

4

Beta strand

37 – 44

8

Helix

45 – 47

3

Helix

48 – 54

7

Beta strand

55 – 57

3

Beta strand

59 – 63

5

Helix

64 – 71

8

Turn

72 – 74

3

Beta strand

77 – 84

8

Beta strand

89 – 94

6

Turn

95 – 98

4

Helix

103 – 106

4

Beta strand

110 – 113

4

Helix

117 – 127

11

Turn

128 – 130

3

Beta strand

135 – 140

6

Helix

142 – 144

3

Helix

145 – 150

6

Beta strand

153 – 160

8

Turn

161 – 163

3

Helix

164 – 174

11

Helix

179 – 181

3

Beta strand

182 – 186

5

Helix

196 – 199

4

Beta strand

201 – 206

6

Helix

207 – 212

6

Helix

214 – 233

20

Helix

235 – 245

11

Helix

247 – 249

3

Helix

252 – 261

10

Turn

262 – 264

3

Helix

273 – 285

13

Helix

315 – 330

16

Sequences

	Sequence		Length	Mass (Da)
	P43534 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D810C5D5A86FA6C0	FASTA	340	38,592
	10 20 30 40 50 60 MSTDKITFLL NWQPTPYHIP IFLAQTKGYF KEQGLDMAIL EPTNPSDVTE LIGSGKVDMG 70 80 90 100 110 120 LKAMIHTLAA KARGFPVTSV ASLLDEPFTG VLYLKGSGIT EDFQSLKGKK IGYVGEFGKI 130 140 150 160 170 180 QIDELTKHYG MKPEDYTAVR CGMNVAKYII EGKIDAGIGI ECMQQVELEE YLAKQGRPAS 190 200 210 220 230 240 DAKMLRIDKL ACLGCCCFCT VLYICNDEFL KKNPEKVRKF LKAIKKATDY VLADPVKAWK 250 260 270 280 290 300 EYIDFKPQLN NDLSYKQYQR CYAYFSSSLY NVHRDWKKVT GYGKRLAILP PDYVSNYTNE 310 320 330 340 YLSWPEPEEV SDPLEAQRLM AIHQEKCRQE GTFKRLALPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hather R.J., Praekelt U., Meacock P.A. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine." Wightman R., Meacock P.A. Microbiology 149:1447-1460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z48220 Genomic DNA. Translation: CAA88253.1.
D50617 Genomic DNA. Translation: BAA09183.1.
BK006940 Genomic DNA. Translation: DAA12382.1.

RefSeq

NP_116597.1. NM_001179909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4H65	X-ray	2.60	A/B	1-340	[»]
4H67	X-ray	2.70	A/B/C/D/E/F/G/H	1-340	[»]
4H6D	X-ray	2.90	A/B/C/D/E/F/G/H	1-340	[»]

ProteinModelPortal

P43534.

SMR

P43534. Positions 4-338.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3951N.

IntAct

P43534. 1 interaction.

MINT

MINT-525063.

STRING

4932.YFL058W.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFL058W; YFL058W; YFL058W.

GeneID

850486.

KEGG

sce:YFL058W.

Organism-specific databases

CYGD

YFL058w.

SGD

S000001836. THI5.

Phylogenomic databases

GeneTree

ENSGT00390000017029.

HOGENOM

HOG000160891.

OMA

GAYRNFE.

OrthoDB

EOG4SN4XF.

Gene expression databases

Genevestigator

P43534.

GermOnline

YFL058W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR015168. NMT1.
[Graphical view]

Pfam

PF09084. NMT1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

966152.

Entry information

Entry name

THI5_YEAST

Accession

Primary (citable) accession number: P43534
Secondary accession number(s): D6VTH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program