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P43534 (THI5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyrimidine precursor biosynthesis enzyme THI5
Gene names
Name:THI5
Ordered Locus Names:YFL058W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety. Ref.4

Sequence similarities

Belongs to the NMT1 family.

Ontologies

Keywords
   Biological processThiamine biosynthesis
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processthiamine biosynthetic process

Inferred from genetic interaction Ref.4. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RSP5P399402EBI-19221,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Pyrimidine precursor biosynthesis enzyme THI5
PRO_0000211618

Secondary structure

............................................................. 340
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43534 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D810C5D5A86FA6C0

FASTA34038,592
        10         20         30         40         50         60 
MSTDKITFLL NWQPTPYHIP IFLAQTKGYF KEQGLDMAIL EPTNPSDVTE LIGSGKVDMG 

        70         80         90        100        110        120 
LKAMIHTLAA KARGFPVTSV ASLLDEPFTG VLYLKGSGIT EDFQSLKGKK IGYVGEFGKI 

       130        140        150        160        170        180 
QIDELTKHYG MKPEDYTAVR CGMNVAKYII EGKIDAGIGI ECMQQVELEE YLAKQGRPAS 

       190        200        210        220        230        240 
DAKMLRIDKL ACLGCCCFCT VLYICNDEFL KKNPEKVRKF LKAIKKATDY VLADPVKAWK 

       250        260        270        280        290        300 
EYIDFKPQLN NDLSYKQYQR CYAYFSSSLY NVHRDWKKVT GYGKRLAILP PDYVSNYTNE 

       310        320        330        340 
YLSWPEPEEV SDPLEAQRLM AIHQEKCRQE GTFKRLALPA 

« Hide

References

« Hide 'large scale' references
[1]Hather R.J., Praekelt U., Meacock P.A.
Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine."
Wightman R., Meacock P.A.
Microbiology 149:1447-1460(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z48220 Genomic DNA. Translation: CAA88253.1.
D50617 Genomic DNA. Translation: BAA09183.1.
BK006940 Genomic DNA. Translation: DAA12382.1.
RefSeqNP_116597.1. NM_001179909.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4H65X-ray2.60A/B1-340[»]
4H67X-ray2.70A/B/C/D/E/F/G/H1-340[»]
4H6DX-ray2.90A/B/C/D/E/F/G/H1-340[»]
ProteinModelPortalP43534.
SMRP43534. Positions 4-338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31089. 2 interactions.
DIPDIP-3951N.
IntActP43534. 1 interaction.
MINTMINT-525063.
STRING4932.YFL058W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFL058W; YFL058W; YFL058W.
GeneID850486.
KEGGsce:YFL058W.

Organism-specific databases

CYGDYFL058w.
SGDS000001836. THI5.

Phylogenomic databases

GeneTreeENSGT00390000017029.
HOGENOMHOG000160891.
OMAEIEMIEP.
OrthoDBEOG7DRJD1.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER3O-3915.
YEAST:MONOMER3O-3915.

Gene expression databases

GenevestigatorP43534.

Family and domain databases

InterProIPR027939. NMT1/THI5.
IPR015168. SsuA/THI5.
[Graphical view]
PANTHERPTHR31528. PTHR31528. 1 hit.
PfamPF09084. NMT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966152.

Entry information

Entry nameTHI5_YEAST
AccessionPrimary (citable) accession number: P43534
Secondary accession number(s): D6VTH2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references