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P43534

- THI5_YEAST

UniProt

P43534 - THI5_YEAST

Protein

Pyrimidine precursor biosynthesis enzyme THI5

Gene

THI5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. thiamine biosynthetic process Source: SGD

    Keywords - Biological processi

    Thiamine biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-3915.
    YEAST:MONOMER3O-3915.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyrimidine precursor biosynthesis enzyme THI5
    Gene namesi
    Name:THI5
    Ordered Locus Names:YFL058W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL058w.
    SGDiS000001836. THI5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 340340Pyrimidine precursor biosynthesis enzyme THI5PRO_0000211618Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP43534.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSP5P399402EBI-19221,EBI-16219

    Protein-protein interaction databases

    BioGridi31089. 2 interactions.
    DIPiDIP-3951N.
    IntActiP43534. 1 interaction.
    MINTiMINT-525063.
    STRINGi4932.YFL058W.

    Structurei

    Secondary structure

    1
    340
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 94
    Beta strandi11 – 133
    Helixi16 – 183
    Helixi19 – 268
    Helixi29 – 324
    Beta strandi37 – 448
    Helixi45 – 473
    Helixi48 – 547
    Beta strandi55 – 573
    Beta strandi59 – 635
    Helixi64 – 718
    Turni72 – 743
    Beta strandi77 – 848
    Beta strandi89 – 946
    Turni95 – 984
    Helixi103 – 1064
    Beta strandi110 – 1134
    Helixi117 – 12711
    Turni128 – 1303
    Beta strandi135 – 1406
    Helixi142 – 1443
    Helixi145 – 1506
    Beta strandi153 – 1608
    Turni161 – 1633
    Helixi164 – 17411
    Helixi179 – 1813
    Beta strandi182 – 1865
    Helixi196 – 1994
    Beta strandi201 – 2066
    Helixi207 – 2126
    Helixi214 – 23320
    Helixi235 – 24511
    Helixi247 – 2493
    Helixi252 – 26110
    Turni262 – 2643
    Helixi273 – 28513
    Helixi315 – 33016

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4H65X-ray2.60A/B1-340[»]
    4H67X-ray2.70A/B/C/D/E/F/G/H1-340[»]
    4H6DX-ray2.90A/B/C/D/E/F/G/H1-340[»]
    ProteinModelPortaliP43534.
    SMRiP43534. Positions 4-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NMT1 family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000017029.
    HOGENOMiHOG000160891.
    KOiK18278.
    OMAiGGYINHE.
    OrthoDBiEOG7DRJD1.

    Family and domain databases

    InterProiIPR027939. NMT1/THI5.
    IPR015168. SsuA/THI5.
    [Graphical view]
    PANTHERiPTHR31528. PTHR31528. 1 hit.
    PfamiPF09084. NMT1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43534-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTDKITFLL NWQPTPYHIP IFLAQTKGYF KEQGLDMAIL EPTNPSDVTE    50
    LIGSGKVDMG LKAMIHTLAA KARGFPVTSV ASLLDEPFTG VLYLKGSGIT 100
    EDFQSLKGKK IGYVGEFGKI QIDELTKHYG MKPEDYTAVR CGMNVAKYII 150
    EGKIDAGIGI ECMQQVELEE YLAKQGRPAS DAKMLRIDKL ACLGCCCFCT 200
    VLYICNDEFL KKNPEKVRKF LKAIKKATDY VLADPVKAWK EYIDFKPQLN 250
    NDLSYKQYQR CYAYFSSSLY NVHRDWKKVT GYGKRLAILP PDYVSNYTNE 300
    YLSWPEPEEV SDPLEAQRLM AIHQEKCRQE GTFKRLALPA 340
    Length:340
    Mass (Da):38,592
    Last modified:November 1, 1995 - v1
    Checksum:iD810C5D5A86FA6C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48220 Genomic DNA. Translation: CAA88253.1.
    D50617 Genomic DNA. Translation: BAA09183.1.
    BK006940 Genomic DNA. Translation: DAA12382.1.
    RefSeqiNP_116597.1. NM_001179909.1.

    Genome annotation databases

    EnsemblFungiiYFL058W; YFL058W; YFL058W.
    GeneIDi850486.
    KEGGisce:YFL058W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48220 Genomic DNA. Translation: CAA88253.1 .
    D50617 Genomic DNA. Translation: BAA09183.1 .
    BK006940 Genomic DNA. Translation: DAA12382.1 .
    RefSeqi NP_116597.1. NM_001179909.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4H65 X-ray 2.60 A/B 1-340 [» ]
    4H67 X-ray 2.70 A/B/C/D/E/F/G/H 1-340 [» ]
    4H6D X-ray 2.90 A/B/C/D/E/F/G/H 1-340 [» ]
    ProteinModelPortali P43534.
    SMRi P43534. Positions 4-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31089. 2 interactions.
    DIPi DIP-3951N.
    IntActi P43534. 1 interaction.
    MINTi MINT-525063.
    STRINGi 4932.YFL058W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL058W ; YFL058W ; YFL058W .
    GeneIDi 850486.
    KEGGi sce:YFL058W.

    Organism-specific databases

    CYGDi YFL058w.
    SGDi S000001836. THI5.

    Phylogenomic databases

    GeneTreei ENSGT00390000017029.
    HOGENOMi HOG000160891.
    KOi K18278.
    OMAi GGYINHE.
    OrthoDBi EOG7DRJD1.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER3O-3915.
    YEAST:MONOMER3O-3915.

    Miscellaneous databases

    NextBioi 966152.

    Gene expression databases

    Genevestigatori P43534.

    Family and domain databases

    InterProi IPR027939. NMT1/THI5.
    IPR015168. SsuA/THI5.
    [Graphical view ]
    PANTHERi PTHR31528. PTHR31528. 1 hit.
    Pfami PF09084. NMT1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Hather R.J., Praekelt U., Meacock P.A.
      Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine."
      Wightman R., Meacock P.A.
      Microbiology 149:1447-1460(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiTHI5_YEAST
    AccessioniPrimary (citable) accession number: P43534
    Secondary accession number(s): D6VTH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3