ID   ELAH_ECOH1              Reviewed;         258 AA.
AC   P43530; D0Z6T2;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 98.
DE   RecName: Full=Heat-labile enterotoxin A chain;
DE   AltName: Full=LT-A, human;
DE            Short=LTH-A;
DE   Flags: Precursor;
GN   Name=eltA; Synonyms=ltpA, toxA; OrderedLocusNames=ETEC_p666_0660;
OS   Escherichia coli O78:H11 (strain H10407 / ETEC).
OG   Plasmid pEntH10407, and Plasmid p666.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=316401;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H10407 / ETEC;
RX   PubMed=6325417; DOI=10.1016/s0021-9258(17)42952-8;
RA   Yamamoto T., Tamura T., Yokota T.;
RT   "Primary structure of heat-labile enterotoxin produced by Escherichia coli
RT   pathogenic for humans.";
RL   J. Biol. Chem. 259:5037-5044(1984).
RN   [2]
RP   SEQUENCE REVISION TO 207.
RC   STRAIN=H10407 / ETEC;
RX   PubMed=3546273; DOI=10.1128/jb.169.3.1352-1357.1987;
RA   Yamamoto T., Gojobori T., Yokota T.;
RT   "Evolutionary origin of pathogenic determinants in enterotoxigenic
RT   Escherichia coli and Vibrio cholerae O1.";
RL   J. Bacteriol. 169:1352-1357(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H10407 / ETEC;
RX   PubMed=8486242; DOI=10.1111/j.1574-6968.1993.tb06092.x;
RA   Inoue T., Tsuji T., Koto M., Imamura S., Miyama A.;
RT   "Amino acid sequence of heat-labile enterotoxin from chicken
RT   enterotoxigenic Escherichia coli is identical to that of human strain H
RT   10407.";
RL   FEMS Microbiol. Lett. 108:157-161(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H10407 / ETEC; PLASMID=pEntH10407;
RX   PubMed=19767599; DOI=10.1093/dnares/dsp015;
RA   Ochi S., Shimizu T., Ohtani K., Ichinose Y., Arimitsu H., Tsukamoto K.,
RA   Kato M., Tsuji T.;
RT   "Nucleotide sequence analysis of the enterotoxigenic Escherichia coli Ent
RT   plasmid.";
RL   DNA Res. 16:299-309(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H10407 / ETEC; PLASMID=p666;
RX   PubMed=20802035; DOI=10.1128/jb.00710-10;
RA   Crossman L.C., Chaudhuri R.R., Beatson S.A., Wells T.J., Desvaux M.,
RA   Cunningham A.F., Petty N.K., Mahon V., Brinkley C., Hobman J.L.,
RA   Savarino S.J., Turner S.M., Pallen M.J., Penn C.W., Parkhill J.,
RA   Turner A.K., Johnson T.J., Thomson N.R., Smith S.G., Henderson I.R.;
RT   "A commensal gone bad: complete genome sequence of the prototypical
RT   enterotoxigenic Escherichia coli strain H10407.";
RL   J. Bacteriol. 192:5822-5831(2010).
RN   [6]
RP   DISCUSSION OF SEQUENCE.
RX   PubMed=7623669; DOI=10.1111/j.1365-2958.1995.tb02289.x;
RA   Domenighini M., Pizza M., Jobling M.G., Holmes R.K., Rappuoli R.;
RT   "Identification of errors among database sequence entries and comparison of
RT   correct amino acid sequences for the heat-labile enterotoxins of
RT   Escherichia coli and Vibrio cholerae.";
RL   Mol. Microbiol. 15:1165-1167(1995).
CC   -!- FUNCTION: The biological activity of the toxin is produced by the A
CC       chain, which activates intracellular adenyl cyclase.
CC   -!- SUBUNIT: Heterohexamer of one A chain and of five B chains.
CC   -!- SIMILARITY: Belongs to the enterotoxin A family. {ECO:0000305}.
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DR   EMBL; K01995; AAA24685.1; -; Genomic_DNA.
DR   EMBL; S60731; AAC60440.2; -; Genomic_DNA.
DR   EMBL; AP010910; BAI49223.1; -; Genomic_DNA.
DR   EMBL; FN649417; CBJ04426.1; -; Genomic_DNA.
DR   PIR; I55231; QLECA.
DR   RefSeq; WP_001426678.1; NC_017722.1.
DR   RefSeq; YP_003293997.1; NC_013507.1.
DR   AlphaFoldDB; P43530; -.
DR   SMR; P43530; -.
DR   TCDB; 1.C.72.4.1; the pertussis toxin (ptx) family.
DR   KEGG; elh:ETEC_p666_0660; -.
DR   HOGENOM; CLU_091751_0_0_6; -.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.210.10; Heat-Labile Enterotoxin, subunit A; 1.
DR   InterPro; IPR001144; Enterotoxin_A.
DR   Pfam; PF01375; Enterotoxin_a; 1.
DR   PRINTS; PR00771; ENTEROTOXINA.
DR   SUPFAM; SSF56399; ADP-ribosylation; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Enterotoxin; Plasmid; Signal; Toxin; Virulence.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000250"
FT   CHAIN           19..258
FT                   /note="Heat-labile enterotoxin A chain"
FT                   /id="PRO_0000019352"
FT   ACT_SITE        128
FT                   /evidence="ECO:0000250"
FT   BINDING         25..39
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   DISULFID        205..217
FT                   /evidence="ECO:0000250"
FT   CONFLICT        207
FT                   /note="N -> D (in Ref. 1; AAA24685 and 3; AAC60440)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   258 AA;  29872 MW;  2BB153C777FD78B7 CRC64;
     MKNITFIFFI LLASPLYANG DKLYRADSRP PDEIKRSGGL MPRGHNEYFD RGTQMNINLY
     DHARGTQTGF VRYDDGYVST SLSLRSAHLA GQSILSGYST YYIYVIATAP NMFNVNDVLG
     VYSPHPYEQE VSALGGIPYS QIYGWYRVNF GVIDERLHRN REYRDRYYRN LNIAPAEDGY
     RLAGFPPDHQ AWREEPWIHH APQGCGNSSR TITGDTCNEE TQNLSTIYLR KYQSKVKRQI
     FSDYQSEVDI YNRIRNEL
//