P43530 (ELAH_ECOH1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat-labile enterotoxin A chain

Alternative name(s):
LT-A, human
Short name=LTH-A

Gene names

Name:	eltA
Synonyms:	ltpA, toxA
Ordered Locus Names:	ETEC_p666_0660

Encoded on

Plasmid pEntH10407 Ref.4
Plasmid p666 Ref.5

Organism

Escherichia coli O78:H11 (strain H10407 / ETEC) [Complete proteome] [HAMAP]

Taxonomic identifier

316401 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.
Subunit structure	Heterohexamer of one A chain and of five B chains.
Sequence similarities	Belongs to the enterotoxin A family.

Ontologies

Keywords
Domain	Signal
Molecular function	Enterotoxin Toxin
PTM	Disulfide bond
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular_function	catalytic activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Chain	19 – 258	240	Heat-labile enterotoxin A chain		PRO_0000019352
Regions
	Nucleotide binding	25 – 39	15	NAD By similarity
Sites
	Active site	128	1	By similarity
Amino acid modifications
	Disulfide bond	205 ↔ 217		By similarity
Experimental info
	Sequence conflict	207	1	N → D in AAA24685. Ref.1
	Sequence conflict	207	1	N → D in AAC60440. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P43530 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2BB153C777FD78B7	FASTA	258	29,872
	10 20 30 40 50 60 MKNITFIFFI LLASPLYANG DKLYRADSRP PDEIKRSGGL MPRGHNEYFD RGTQMNINLY 70 80 90 100 110 120 DHARGTQTGF VRYDDGYVST SLSLRSAHLA GQSILSGYST YYIYVIATAP NMFNVNDVLG 130 140 150 160 170 180 VYSPHPYEQE VSALGGIPYS QIYGWYRVNF GVIDERLHRN REYRDRYYRN LNIAPAEDGY 190 200 210 220 230 240 RLAGFPPDHQ AWREEPWIHH APQGCGNSSR TITGDTCNEE TQNLSTIYLR KYQSKVKRQI 250 FSDYQSEVDI YNRIRNEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of heat-labile enterotoxin produced by Escherichia coli pathogenic for humans." Yamamoto T., Tamura T., Yokota T. J. Biol. Chem. 259:5037-5044(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: H10407 / ETEC.
[2]	"Evolutionary origin of pathogenic determinants in enterotoxigenic Escherichia coli and Vibrio cholerae O1." Yamamoto T., Gojobori T., Yokota T. J. Bacteriol. 169:1352-1357(1987) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 207. Strain: H10407 / ETEC.
[3]	"Amino acid sequence of heat-labile enterotoxin from chicken enterotoxigenic Escherichia coli is identical to that of human strain H 10407." Inoue T., Tsuji T., Koto M., Imamura S., Miyama A. FEMS Microbiol. Lett. 108:157-161(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: H10407 / ETEC.
[4]	"Nucleotide sequence analysis of the enterotoxigenic Escherichia coli Ent plasmid." Ochi S., Shimizu T., Ohtani K., Ichinose Y., Arimitsu H., Tsukamoto K., Kato M., Tsuji T. DNA Res. 16:299-309(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H10407 / ETEC.
[5]	"A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407." Crossman L.C., Chaudhuri R.R., Beatson S.A., Wells T.J., Desvaux M., Cunningham A.F., Petty N.K., Mahon V., Brinkley C., Hobman J.L., Savarino S.J., Turner S.M., Pallen M.J., Penn C.W., Parkhill J., Turner A.K., Johnson T.J., Thomson N.R., Smith S.G., Henderson I.R. J. Bacteriol. 192:5822-5831(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: H10407 / ETEC.
[6]	"Identification of errors among database sequence entries and comparison of correct amino acid sequences for the heat-labile enterotoxins of Escherichia coli and Vibrio cholerae." Domenighini M., Pizza M., Jobling M.G., Holmes R.K., Rappuoli R. Mol. Microbiol. 15:1165-1167(1995) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K01995 Genomic DNA. Translation: AAA24685.1. S60731 Genomic DNA. Translation: AAC60440.2. AP010910 Genomic DNA. Translation: BAI49223.1. FN649417 Genomic DNA. Translation: CBJ04426.1.
PIR	QLECA. I55231.
RefSeq	YP_003293997.1. NC_013507.1. YP_006203800.1. NC_017722.1.
3D structure databases
ProteinModelPortal	P43530.
SMR	P43530. Positions 22-254.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBJ04426; CBJ04426; ETEC_p666_0660.
GeneID	12796728. 8571459.
KEGG	elh:ETEC_p666_0660.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K10928.
Family and domain databases
InterPro	IPR001144. Enterotoxin_A. [Graphical view]
Pfam	PF01375. Enterotoxin_a. 1 hit. [Graphical view]
PRINTS	PR00771. ENTEROTOXINA.
ProtoNet	Search...

Entry information

Entry name

ELAH_ECOH1

Accession

Primary (citable) accession number: P43530
Secondary accession number(s): D0Z6T2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program