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Protein

Heat-labile enterotoxin A chain

Gene

eltA

Organism
Escherichia coli O78:H11 (strain H10407 / ETEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei128 – 1281By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 3915NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Enterotoxin, Toxin

Enzyme and pathway databases

BioCyciECOL316401:GLD0-5049-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat-labile enterotoxin A chain
Alternative name(s):
LT-A, human
Short name:
LTH-A
Gene namesi
Name:eltA
Synonyms:ltpA, toxA
Ordered Locus Names:ETEC_p666_0660
Encoded oniPlasmid pEntH104071 Publication
Plasmid p6661 Publication
OrganismiEscherichia coli O78:H11 (strain H10407 / ETEC)
Taxonomic identifieri316401 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000006877 Componenti: Plasmid p666

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 258240Heat-labile enterotoxin A chainPRO_0000019352Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi205 ↔ 217By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterohexamer of one A chain and of five B chains.

Structurei

3D structure databases

ProteinModelPortaliP43530.
SMRiP43530. Positions 22-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the enterotoxin A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK10928.
OMAiNITFIFF.

Family and domain databases

InterProiIPR001144. Enterotoxin_A.
[Graphical view]
PfamiPF01375. Enterotoxin_a. 1 hit.
[Graphical view]
PRINTSiPR00771. ENTEROTOXINA.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNITFIFFI LLASPLYANG DKLYRADSRP PDEIKRSGGL MPRGHNEYFD
60 70 80 90 100
RGTQMNINLY DHARGTQTGF VRYDDGYVST SLSLRSAHLA GQSILSGYST
110 120 130 140 150
YYIYVIATAP NMFNVNDVLG VYSPHPYEQE VSALGGIPYS QIYGWYRVNF
160 170 180 190 200
GVIDERLHRN REYRDRYYRN LNIAPAEDGY RLAGFPPDHQ AWREEPWIHH
210 220 230 240 250
APQGCGNSSR TITGDTCNEE TQNLSTIYLR KYQSKVKRQI FSDYQSEVDI

YNRIRNEL
Length:258
Mass (Da):29,872
Last modified:November 1, 1995 - v1
Checksum:i2BB153C777FD78B7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti207 – 2071N → D in AAA24685 (PubMed:6325417).Curated
Sequence conflicti207 – 2071N → D in AAC60440 (PubMed:8486242).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01995 Genomic DNA. Translation: AAA24685.1.
S60731 Genomic DNA. Translation: AAC60440.2.
AP010910 Genomic DNA. Translation: BAI49223.1.
FN649417 Genomic DNA. Translation: CBJ04426.1.
PIRiI55231. QLECA.
RefSeqiWP_001426678.1. NC_017722.1.
YP_003293997.1. NC_013507.1.
YP_006203800.1. NC_017722.1.

Genome annotation databases

EnsemblBacteriaiCBJ04426; CBJ04426; ETEC_p666_0660.
GeneIDi8571459.
KEGGielh:ETEC_p666_0660.
pg:8571459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01995 Genomic DNA. Translation: AAA24685.1.
S60731 Genomic DNA. Translation: AAC60440.2.
AP010910 Genomic DNA. Translation: BAI49223.1.
FN649417 Genomic DNA. Translation: CBJ04426.1.
PIRiI55231. QLECA.
RefSeqiWP_001426678.1. NC_017722.1.
YP_003293997.1. NC_013507.1.
YP_006203800.1. NC_017722.1.

3D structure databases

ProteinModelPortaliP43530.
SMRiP43530. Positions 22-254.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBJ04426; CBJ04426; ETEC_p666_0660.
GeneIDi8571459.
KEGGielh:ETEC_p666_0660.
pg:8571459.

Phylogenomic databases

KOiK10928.
OMAiNITFIFF.

Enzyme and pathway databases

BioCyciECOL316401:GLD0-5049-MONOMER.

Family and domain databases

InterProiIPR001144. Enterotoxin_A.
[Graphical view]
PfamiPF01375. Enterotoxin_a. 1 hit.
[Graphical view]
PRINTSiPR00771. ENTEROTOXINA.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of heat-labile enterotoxin produced by Escherichia coli pathogenic for humans."
    Yamamoto T., Tamura T., Yokota T.
    J. Biol. Chem. 259:5037-5044(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: H10407 / ETEC.
  2. "Evolutionary origin of pathogenic determinants in enterotoxigenic Escherichia coli and Vibrio cholerae O1."
    Yamamoto T., Gojobori T., Yokota T.
    J. Bacteriol. 169:1352-1357(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 207.
    Strain: H10407 / ETEC.
  3. "Amino acid sequence of heat-labile enterotoxin from chicken enterotoxigenic Escherichia coli is identical to that of human strain H 10407."
    Inoue T., Tsuji T., Koto M., Imamura S., Miyama A.
    FEMS Microbiol. Lett. 108:157-161(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: H10407 / ETEC.
  4. "Nucleotide sequence analysis of the enterotoxigenic Escherichia coli Ent plasmid."
    Ochi S., Shimizu T., Ohtani K., Ichinose Y., Arimitsu H., Tsukamoto K., Kato M., Tsuji T.
    DNA Res. 16:299-309(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H10407 / ETEC.
    Plasmid: pEntH10407
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H10407 / ETEC.
    Plasmid: p666
  6. "Identification of errors among database sequence entries and comparison of correct amino acid sequences for the heat-labile enterotoxins of Escherichia coli and Vibrio cholerae."
    Domenighini M., Pizza M., Jobling M.G., Holmes R.K., Rappuoli R.
    Mol. Microbiol. 15:1165-1167(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiELAH_ECOH1
AccessioniPrimary (citable) accession number: P43530
Secondary accession number(s): D0Z6T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 27, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.