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P43528 (E2BA_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heat-labile enterotoxin IIB, A chain

Short name=LT-IIB
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.

Subunit structure

Heterohexamer of one A chain and of five B chains.

Sequence similarities

Belongs to the enterotoxin A family.

Ontologies

Keywords
   DomainSignal
   Molecular functionEnterotoxin
Toxin
   PTMDisulfide bond
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncatalytic activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 263243Heat-labile enterotoxin IIB, A chain
PRO_0000019354

Regions

Nucleotide binding26 – 3914NAD

Sites

Active site1301

Amino acid modifications

Disulfide bond205 ↔ 217

Secondary structure

...................................... 263
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43528 [UniParc].

Last modified September 5, 2012. Version 2.
Checksum: 4F648DBDF99CA791

FASTA26329,519
        10         20         30         40         50         60 
MAKVISFFIS LFLISFPLYA NDYFRADSRT PDEVRRSGGL IPRGQDEAYE RGTPININLY 

        70         80         90        100        110        120 
DHARGTATGN TRYNDGYVST TTTLRQAHFL GQNMLGGYNE YYIYVVAAAP NLFDVNGVLG 

       130        140        150        160        170        180 
RYSPYPSENE YAALGGIPLS QIIGWYRVSF GAIEGGMHRN RDYRRDLFRG LSAAPNEDGY 

       190        200        210        220        230        240 
RIAGFPDGFP AWEEVPWREF APNSCLPNNK ASSDTTCASL TNKLSQHDLA DFKKYIKRKF 

       250        260 
TLMTLLSINN DGFFSNNGGK DEL 

« Hide

References

[1]"Cloning, nucleotide sequence, and hybridization studies of the type IIb heat-labile enterotoxin gene of Escherichia coli."
Pickett C.L., Twiddy E.M., Coker C., Holmes R.K.
J. Bacteriol. 171:4945-4952(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Isolate 41.
[2]Jobling M.G., Holmes R.K.
Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 89.
[3]"Crystal structure of a new heat-labile enterotoxin, LT-IIb."
van den Akker F., Sarfaty S., Twiddy E.M., Connell T.D., Holmes R.K., Hol W.G.J.
Structure 4:665-678(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JQ031712 Genomic DNA. Translation: AAA53285.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1TIIX-ray2.25A21-210[»]
C211-263[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-6216N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001144. Enterotoxin_A.
[Graphical view]
PfamPF01375. Enterotoxin_a. 1 hit.
[Graphical view]
PRINTSPR00771. ENTEROTOXINA.
ProtoNetSearch...

Other

EvolutionaryTraceP43528.

Entry information

Entry nameE2BA_ECOLX
AccessionPrimary (citable) accession number: P43528
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 5, 2012
Last modified: April 16, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references