P43528 (E2BA_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Heat-labile enterotoxin IIB, A chain Short name=LT-IIB
Organism	Escherichia coli
Taxonomic identifier	562 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.
Subunit structure	Heterohexamer of one A chain and of five B chains.
Sequence similarities	Belongs to the enterotoxin A family.

Ontologies

Keywords
Domain	Signal
Molecular function	Enterotoxin Toxin
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular_function	catalytic activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Chain

21 – 263

243

Heat-labile enterotoxin IIB, A chain

PRO_0000019354

Regions

Nucleotide binding

26 – 39

14

NAD

Sites

Active site

130

1

Amino acid modifications

Disulfide bond

205 ↔ 217

Secondary structure

1

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263

Helix Strand Turn

Details...

Beta strand

22 – 29

8

Helix

31 – 37

7

Turn

49 – 51

3

Helix

59 – 64

6

Beta strand

70 – 72

3

Helix

84 – 94

11

Beta strand

99 – 107

9

Beta strand

112 – 114

3

Helix

115 – 119

5

Helix

120 – 122

3

Helix

126 – 128

3

Beta strand

131 – 134

4

Helix

139 – 141

3

Beta strand

142 – 149

8

Helix

165 – 168

4

Helix

176 – 180

5

Helix

190 – 193

4

Helix

197 – 199

3

Helix

203 – 205

3

Helix

217 – 248

32

Sequences

	Sequence		Length	Mass (Da)
	P43528 [UniParc]. Last modified September 5, 2012. Version 2. Checksum: 4F648DBDF99CA791	FASTA	263	29,519
	10 20 30 40 50 60 MAKVISFFIS LFLISFPLYA NDYFRADSRT PDEVRRSGGL IPRGQDEAYE RGTPININLY 70 80 90 100 110 120 DHARGTATGN TRYNDGYVST TTTLRQAHFL GQNMLGGYNE YYIYVVAAAP NLFDVNGVLG 130 140 150 160 170 180 RYSPYPSENE YAALGGIPLS QIIGWYRVSF GAIEGGMHRN RDYRRDLFRG LSAAPNEDGY 190 200 210 220 230 240 RIAGFPDGFP AWEEVPWREF APNSCLPNNK ASSDTTCASL TNKLSQHDLA DFKKYIKRKF 250 260 TLMTLLSINN DGFFSNNGGK DEL

References

[1]	"Cloning, nucleotide sequence, and hybridization studies of the type IIb heat-labile enterotoxin gene of Escherichia coli." Pickett C.L., Twiddy E.M., Coker C., Holmes R.K. J. Bacteriol. 171:4945-4952(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Isolate 41.
[2]	Jobling M.G., Holmes R.K. Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 89.
[3]	"Crystal structure of a new heat-labile enterotoxin, LT-IIb." van den Akker F., Sarfaty S., Twiddy E.M., Connell T.D., Holmes R.K., Hol W.G.J. Structure 4:665-678(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

JQ031712 Genomic DNA. Translation: AAA53285.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TII	X-ray	2.25	A	21-210	[»]
			C	211-263	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6216N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001144. Enterotoxin_A.
[Graphical view]

Pfam

PF01375. Enterotoxin_a. 1 hit.
[Graphical view]

PRINTS

PR00771. ENTEROTOXINA.

ProtoNet

Search...

Other

EvolutionaryTrace

P43528.

Entry information

Entry name

E2BA_ECOLX

Accession

Primary (citable) accession number: P43528

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	September 5, 2012
Last modified:	April 3, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program