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P43528

- E2BA_ECOLX

UniProt

P43528 - E2BA_ECOLX

Protein

Heat-labile enterotoxin IIB, A chain

Gene
N/A
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (05 Sep 2012)
      Previous versions | rss
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    Functioni

    The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei130 – 1301

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 3914NADAdd
    BLAST

    GO - Molecular functioni

    1. catalytic activity Source: InterPro

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Enterotoxin, Toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat-labile enterotoxin IIB, A chain
    Short name:
    LT-IIB
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Add
    BLAST
    Chaini21 – 263243Heat-labile enterotoxin IIB, A chainPRO_0000019354Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi205 ↔ 217

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Heterohexamer of one A chain and of five B chains.

    Protein-protein interaction databases

    DIPiDIP-6216N.

    Structurei

    Secondary structure

    1
    263
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi22 – 298
    Helixi31 – 377
    Turni49 – 513
    Helixi59 – 646
    Beta strandi70 – 723
    Helixi84 – 9411
    Beta strandi99 – 1079
    Beta strandi112 – 1143
    Helixi115 – 1195
    Helixi120 – 1223
    Helixi126 – 1283
    Beta strandi131 – 1344
    Helixi139 – 1413
    Beta strandi142 – 1498
    Helixi165 – 1684
    Helixi176 – 1805
    Helixi190 – 1934
    Helixi197 – 1993
    Helixi203 – 2053
    Helixi217 – 24832

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TIIX-ray2.25A21-210[»]
    C211-263[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43528.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the enterotoxin A family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001144. Enterotoxin_A.
    [Graphical view]
    PfamiPF01375. Enterotoxin_a. 1 hit.
    [Graphical view]
    PRINTSiPR00771. ENTEROTOXINA.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43528-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKVISFFIS LFLISFPLYA NDYFRADSRT PDEVRRSGGL IPRGQDEAYE    50
    RGTPININLY DHARGTATGN TRYNDGYVST TTTLRQAHFL GQNMLGGYNE 100
    YYIYVVAAAP NLFDVNGVLG RYSPYPSENE YAALGGIPLS QIIGWYRVSF 150
    GAIEGGMHRN RDYRRDLFRG LSAAPNEDGY RIAGFPDGFP AWEEVPWREF 200
    APNSCLPNNK ASSDTTCASL TNKLSQHDLA DFKKYIKRKF TLMTLLSINN 250
    DGFFSNNGGK DEL 263
    Length:263
    Mass (Da):29,519
    Last modified:September 5, 2012 - v2
    Checksum:i4F648DBDF99CA791
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JQ031712 Genomic DNA. Translation: AAA53285.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JQ031712 Genomic DNA. Translation: AAA53285.2 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TII X-ray 2.25 A 21-210 [» ]
    C 211-263 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6216N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P43528.

    Family and domain databases

    InterProi IPR001144. Enterotoxin_A.
    [Graphical view ]
    Pfami PF01375. Enterotoxin_a. 1 hit.
    [Graphical view ]
    PRINTSi PR00771. ENTEROTOXINA.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, nucleotide sequence, and hybridization studies of the type IIb heat-labile enterotoxin gene of Escherichia coli."
      Pickett C.L., Twiddy E.M., Coker C., Holmes R.K.
      J. Bacteriol. 171:4945-4952(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Isolate 41.
    2. Jobling M.G., Holmes R.K.
      Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 89.
    3. "Crystal structure of a new heat-labile enterotoxin, LT-IIb."
      van den Akker F., Sarfaty S., Twiddy E.M., Connell T.D., Holmes R.K., Hol W.G.J.
      Structure 4:665-678(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).

    Entry informationi

    Entry nameiE2BA_ECOLX
    AccessioniPrimary (citable) accession number: P43528
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: September 5, 2012
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3