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P43527

- CASP1_RAT

UniProt

P43527 - CASP1_RAT

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Protein
Caspase-1
Gene
Casp1, Il1bc, Il1bce
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis By similarity.

Catalytic activityi

Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei236 – 2361 By similarity
Active sitei284 – 2841 By similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: RefGenome
  2. protein binding Source: RGD
  3. scaffold protein binding Source: RGD

GO - Biological processi

  1. apoptotic process Source: RGD
  2. execution phase of apoptosis Source: RefGenome
  3. lung development Source: RGD
  4. memory Source: RGD
  5. microglial cell activation Source: RGD
  6. midgut development Source: RGD
  7. myoblast fusion Source: RGD
  8. positive regulation of apoptotic process Source: RGD
  9. positive regulation of circadian sleep/wake cycle, non-REM sleep Source: RGD
  10. positive regulation of cytokine secretion Source: RGD
  11. positive regulation of interleukin-1 beta secretion Source: RGD
  12. regulation of inflammatory response Source: RefGenome
  13. response to drug Source: RGD
  14. response to hypoxia Source: RGD
  15. response to lipopolysaccharide Source: RGD
  16. response to organic cyclic compound Source: RGD
  17. response to toxic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BRENDAi3.4.22.36. 5301.

Protein family/group databases

MEROPSiC14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-1 (EC:3.4.22.36)
Short name:
CASP-1
Alternative name(s):
Interleukin-1 beta convertase
Short name:
IL-1BC
Interleukin-1 beta-converting enzyme
Short name:
ICE
Short name:
IL-1 beta-converting enzyme
p45
Cleaved into the following 2 chains:
Gene namesi
Name:Casp1
Synonyms:Il1bc, Il1bce
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2274. Casp1.

Subcellular locationi

GO - Cellular componenti

  1. AIM2 inflammasome complex Source: UniProtKB
  2. IPAF inflammasome complex Source: UniProtKB
  3. NLRP1 inflammasome complex Source: UniProtKB
  4. NLRP3 inflammasome complex Source: UniProtKB
  5. cytoplasm Source: RGD
  6. neuron projection Source: RGD
  7. nucleus Source: RGD
  8. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – ?118118 Reviewed prediction
PRO_0000004533Add
BLAST
Chaini?119 – 296178Caspase-1 subunit p20
PRO_0000004534Add
BLAST
Propeptidei297 – 31418 Reviewed prediction
PRO_0000004535Add
BLAST
Chaini315 – 40288Caspase-1 subunit p10
PRO_0000004536Add
BLAST

Post-translational modificationi

The two subunits are derived from the precursor sequence by a autocatalytic mechanism.

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP43527.
PRIDEiP43527.

Expressioni

Gene expression databases

GenevestigatoriP43527.

Interactioni

Subunit structurei

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed bya 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p20 and p10 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18 By similarity.

Protein-protein interaction databases

DIPiDIP-29840N.
STRINGi10116.ENSRNOP00000009993.

Structurei

3D structure databases

ProteinModelPortaliP43527.
SMRiP43527. Positions 2-90, 127-296, 315-402.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9191CARD
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C14A family.
Contains 1 CARD domain.

Phylogenomic databases

eggNOGiNOG326166.
HOGENOMiHOG000234399.
HOVERGENiHBG076981.
InParanoidiP43527.
PhylomeDBiP43527.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProiIPR001315. CARD.
IPR029030. Caspase-like_dom.
IPR017350. Caspase_ICE-type.
IPR011029. DEATH-like_dom.
IPR011600. Pept_C14_caspase.
IPR001309. Pept_C14_ICE_p20.
IPR016129. Pept_C14_ICE_p20_AS.
IPR002138. Pept_C14_p10.
IPR015917. Pept_C14A_p45_core.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF00656. Peptidase_C14. 1 hit.
[Graphical view]
PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00114. CARD. 1 hit.
SM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43527-1 [UniParc]FASTAAdd to Basket

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MADKVLRAKR KQFINSVSVG TINGLLDELL EKRVLNQEEM DTIKLANITV    50
MEKARDLCDH VTKKGPRASQ MFITYICNED CYLAEILELQ SGPSAETVFV 100
TEDSKGGHPF SSETKEKLNK EGGAFPGPSG SLKFCPLEIA QKLWKENHSE 150
IYPIMKTPTR TRLALIICNT DFQHLSRRVG ADVDLREMKL LLQDLGYTVK 200
VKENLTALEM TKELKEFAAC PEHKTSDSTF LVFMSHGLQE GICGITYSNE 250
VADILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKDSVGN 300
SEEGFLTDAI FEDDGIKKAH IEKDFIAFCS STPDNVSWRH PVRGSLFIES 350
LIKHMKEYAW SCDLEDIFRK VRFSFEQPDS RLQMPTTERV TLTKRFYLFP 400
GH 402
Length:402
Mass (Da):45,576
Last modified:November 1, 1995 - v1
Checksum:iD2E8FA2BEA76FD40
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 2521A → G in AAC52259. 1 Publication
Sequence conflicti252 – 2521A → G in AAB35431. 1 Publication
Sequence conflicti256 – 2561K → E in AAC52259. 1 Publication
Sequence conflicti256 – 2561K → E in AAB35431. 1 Publication
Sequence conflicti260 – 2601I → L in AAC52259. 1 Publication
Sequence conflicti260 – 2601I → L in AAB35431. 1 Publication
Sequence conflicti262 – 2621Q → H in AAC52259. 1 Publication
Sequence conflicti262 – 2621Q → H in AAB35431. 1 Publication
Sequence conflicti343 – 3431R → Q in AAC52259. 1 Publication
Sequence conflicti343 – 3431R → Q in AAB35431. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14647 mRNA. Translation: AAA85812.1.
U34621 mRNA. Translation: AAC52259.1.
S79676 mRNA. Translation: AAB35431.1.
UniGeneiRn.37508.

Genome annotation databases

UCSCiRGD:2274. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14647 mRNA. Translation: AAA85812.1 .
U34621 mRNA. Translation: AAC52259.1 .
S79676 mRNA. Translation: AAB35431.1 .
UniGenei Rn.37508.

3D structure databases

ProteinModelPortali P43527.
SMRi P43527. Positions 2-90, 127-296, 315-402.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29840N.
STRINGi 10116.ENSRNOP00000009993.

Protein family/group databases

MEROPSi C14.001.

Proteomic databases

PaxDbi P43527.
PRIDEi P43527.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2274. rat.

Organism-specific databases

RGDi 2274. Casp1.

Phylogenomic databases

eggNOGi NOG326166.
HOGENOMi HOG000234399.
HOVERGENi HBG076981.
InParanoidi P43527.
PhylomeDBi P43527.

Enzyme and pathway databases

BRENDAi 3.4.22.36. 5301.

Miscellaneous databases

PROi P43527.

Gene expression databases

Genevestigatori P43527.

Family and domain databases

Gene3Di 1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProi IPR001315. CARD.
IPR029030. Caspase-like_dom.
IPR017350. Caspase_ICE-type.
IPR011029. DEATH-like_dom.
IPR011600. Pept_C14_caspase.
IPR001309. Pept_C14_ICE_p20.
IPR016129. Pept_C14_ICE_p20_AS.
IPR002138. Pept_C14_p10.
IPR015917. Pept_C14A_p45_core.
[Graphical view ]
Pfami PF00619. CARD. 1 hit.
PF00656. Peptidase_C14. 1 hit.
[Graphical view ]
PIRSFi PIRSF038001. Caspase_ICE. 1 hit.
PRINTSi PR00376. IL1BCENZYME.
SMARTi SM00114. CARD. 1 hit.
SM00115. CASc. 1 hit.
[Graphical view ]
SUPFAMi SSF47986. SSF47986. 1 hit.
PROSITEi PS50209. CARD. 1 hit.
PS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, tissue expression and regulation of rat interleukin 1 beta converting enzyme."
    Keane K.M., Giegel D.A., Lipinski W.J., Callahan M.J., Shivers B.D.
    Cytokine 7:105-110(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "Interleukin-1 beta-converting enzyme-related proteases (IRPs) and mammalian cell death: dissociation of IRP-induced oligonucleosomal endonuclease activity from morphological apoptosis in granulosa cells of the ovarian follicle."
    Flaws J.A., Kugu K., Trbovich A.M., Desanti A., Tilly K.I., Hirshfield A.N., Tilly J.L.
    Endocrinology 136:5042-5053(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 228-362.
    Tissue: Ovary.

Entry informationi

Entry nameiCASP1_RAT
AccessioniPrimary (citable) accession number: P43527
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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