Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P43527

- CASP1_RAT

UniProt

P43527 - CASP1_RAT

Protein

Caspase-1

Gene

Casp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis By similarity.By similarity

    Catalytic activityi

    Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei236 – 2361By similarity
    Active sitei284 – 2841By similarity

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: RefGenome
    2. protein binding Source: RGD
    3. scaffold protein binding Source: RGD

    GO - Biological processi

    1. apoptotic process Source: RGD
    2. execution phase of apoptosis Source: RefGenome
    3. lung development Source: RGD
    4. memory Source: RGD
    5. microglial cell activation Source: RGD
    6. midgut development Source: RGD
    7. myoblast fusion Source: RGD
    8. positive regulation of apoptotic process Source: RGD
    9. positive regulation of circadian sleep/wake cycle, non-REM sleep Source: RGD
    10. positive regulation of cytokine secretion Source: RGD
    11. positive regulation of interleukin-1 beta secretion Source: RGD
    12. regulation of inflammatory response Source: RefGenome
    13. response to drug Source: RGD
    14. response to hypoxia Source: RGD
    15. response to lipopolysaccharide Source: RGD
    16. response to organic cyclic compound Source: RGD
    17. response to toxic substance Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Apoptosis

    Enzyme and pathway databases

    BRENDAi3.4.22.36. 5301.

    Protein family/group databases

    MEROPSiC14.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Caspase-1 (EC:3.4.22.36)
    Short name:
    CASP-1
    Alternative name(s):
    Interleukin-1 beta convertase
    Short name:
    IL-1BC
    Interleukin-1 beta-converting enzyme
    Short name:
    ICE
    Short name:
    IL-1 beta-converting enzyme
    p45
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Casp1
    Synonyms:Il1bc, Il1bce
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2274. Casp1.

    Subcellular locationi

    GO - Cellular componenti

    1. AIM2 inflammasome complex Source: UniProtKB
    2. cytoplasm Source: RGD
    3. IPAF inflammasome complex Source: UniProtKB
    4. neuron projection Source: RGD
    5. NLRP1 inflammasome complex Source: UniProtKB
    6. NLRP3 inflammasome complex Source: UniProtKB
    7. nucleus Source: RGD
    8. protein complex Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – ?118118Sequence AnalysisPRO_0000004533Add
    BLAST
    Chaini?119 – 296178Caspase-1 subunit p20PRO_0000004534Add
    BLAST
    Propeptidei297 – 31418Sequence AnalysisPRO_0000004535Add
    BLAST
    Chaini315 – 40288Caspase-1 subunit p10PRO_0000004536Add
    BLAST

    Post-translational modificationi

    The two subunits are derived from the precursor sequence by a autocatalytic mechanism.

    Keywords - PTMi

    Zymogen

    Proteomic databases

    PaxDbiP43527.
    PRIDEiP43527.

    Expressioni

    Gene expression databases

    GenevestigatoriP43527.

    Interactioni

    Subunit structurei

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed bya 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p20 and p10 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-29840N.
    STRINGi10116.ENSRNOP00000009993.

    Structurei

    3D structure databases

    ProteinModelPortaliP43527.
    SMRiP43527. Positions 2-90, 127-296, 315-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 9191CARDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C14A family.Curated
    Contains 1 CARD domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG326166.
    HOGENOMiHOG000234399.
    HOVERGENiHBG076981.
    InParanoidiP43527.
    PhylomeDBiP43527.

    Family and domain databases

    Gene3Di1.10.533.10. 1 hit.
    3.40.50.1460. 1 hit.
    InterProiIPR001315. CARD.
    IPR029030. Caspase-like_dom.
    IPR017350. Caspase_ICE-type.
    IPR011029. DEATH-like_dom.
    IPR011600. Pept_C14_caspase.
    IPR001309. Pept_C14_ICE_p20.
    IPR016129. Pept_C14_ICE_p20_AS.
    IPR002138. Pept_C14_p10.
    IPR015917. Pept_C14A_p45_core.
    [Graphical view]
    PfamiPF00619. CARD. 1 hit.
    PF00656. Peptidase_C14. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
    PRINTSiPR00376. IL1BCENZYME.
    SMARTiSM00114. CARD. 1 hit.
    SM00115. CASc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47986. SSF47986. 1 hit.
    PROSITEiPS50209. CARD. 1 hit.
    PS01122. CASPASE_CYS. 1 hit.
    PS01121. CASPASE_HIS. 1 hit.
    PS50207. CASPASE_P10. 1 hit.
    PS50208. CASPASE_P20. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43527-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADKVLRAKR KQFINSVSVG TINGLLDELL EKRVLNQEEM DTIKLANITV    50
    MEKARDLCDH VTKKGPRASQ MFITYICNED CYLAEILELQ SGPSAETVFV 100
    TEDSKGGHPF SSETKEKLNK EGGAFPGPSG SLKFCPLEIA QKLWKENHSE 150
    IYPIMKTPTR TRLALIICNT DFQHLSRRVG ADVDLREMKL LLQDLGYTVK 200
    VKENLTALEM TKELKEFAAC PEHKTSDSTF LVFMSHGLQE GICGITYSNE 250
    VADILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKDSVGN 300
    SEEGFLTDAI FEDDGIKKAH IEKDFIAFCS STPDNVSWRH PVRGSLFIES 350
    LIKHMKEYAW SCDLEDIFRK VRFSFEQPDS RLQMPTTERV TLTKRFYLFP 400
    GH 402
    Length:402
    Mass (Da):45,576
    Last modified:November 1, 1995 - v1
    Checksum:iD2E8FA2BEA76FD40
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti252 – 2521A → G in AAC52259. (PubMed:7588240)Curated
    Sequence conflicti252 – 2521A → G in AAB35431. (PubMed:7588240)Curated
    Sequence conflicti256 – 2561K → E in AAC52259. (PubMed:7588240)Curated
    Sequence conflicti256 – 2561K → E in AAB35431. (PubMed:7588240)Curated
    Sequence conflicti260 – 2601I → L in AAC52259. (PubMed:7588240)Curated
    Sequence conflicti260 – 2601I → L in AAB35431. (PubMed:7588240)Curated
    Sequence conflicti262 – 2621Q → H in AAC52259. (PubMed:7588240)Curated
    Sequence conflicti262 – 2621Q → H in AAB35431. (PubMed:7588240)Curated
    Sequence conflicti343 – 3431R → Q in AAC52259. (PubMed:7588240)Curated
    Sequence conflicti343 – 3431R → Q in AAB35431. (PubMed:7588240)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14647 mRNA. Translation: AAA85812.1.
    U34621 mRNA. Translation: AAC52259.1.
    S79676 mRNA. Translation: AAB35431.1.
    UniGeneiRn.37508.

    Genome annotation databases

    UCSCiRGD:2274. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14647 mRNA. Translation: AAA85812.1 .
    U34621 mRNA. Translation: AAC52259.1 .
    S79676 mRNA. Translation: AAB35431.1 .
    UniGenei Rn.37508.

    3D structure databases

    ProteinModelPortali P43527.
    SMRi P43527. Positions 2-90, 127-296, 315-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29840N.
    STRINGi 10116.ENSRNOP00000009993.

    Protein family/group databases

    MEROPSi C14.001.

    Proteomic databases

    PaxDbi P43527.
    PRIDEi P43527.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2274. rat.

    Organism-specific databases

    RGDi 2274. Casp1.

    Phylogenomic databases

    eggNOGi NOG326166.
    HOGENOMi HOG000234399.
    HOVERGENi HBG076981.
    InParanoidi P43527.
    PhylomeDBi P43527.

    Enzyme and pathway databases

    BRENDAi 3.4.22.36. 5301.

    Miscellaneous databases

    PROi P43527.

    Gene expression databases

    Genevestigatori P43527.

    Family and domain databases

    Gene3Di 1.10.533.10. 1 hit.
    3.40.50.1460. 1 hit.
    InterProi IPR001315. CARD.
    IPR029030. Caspase-like_dom.
    IPR017350. Caspase_ICE-type.
    IPR011029. DEATH-like_dom.
    IPR011600. Pept_C14_caspase.
    IPR001309. Pept_C14_ICE_p20.
    IPR016129. Pept_C14_ICE_p20_AS.
    IPR002138. Pept_C14_p10.
    IPR015917. Pept_C14A_p45_core.
    [Graphical view ]
    Pfami PF00619. CARD. 1 hit.
    PF00656. Peptidase_C14. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038001. Caspase_ICE. 1 hit.
    PRINTSi PR00376. IL1BCENZYME.
    SMARTi SM00114. CARD. 1 hit.
    SM00115. CASc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47986. SSF47986. 1 hit.
    PROSITEi PS50209. CARD. 1 hit.
    PS01122. CASPASE_CYS. 1 hit.
    PS01121. CASPASE_HIS. 1 hit.
    PS50207. CASPASE_P10. 1 hit.
    PS50208. CASPASE_P20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, tissue expression and regulation of rat interleukin 1 beta converting enzyme."
      Keane K.M., Giegel D.A., Lipinski W.J., Callahan M.J., Shivers B.D.
      Cytokine 7:105-110(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.
    2. "Interleukin-1 beta-converting enzyme-related proteases (IRPs) and mammalian cell death: dissociation of IRP-induced oligonucleosomal endonuclease activity from morphological apoptosis in granulosa cells of the ovarian follicle."
      Flaws J.A., Kugu K., Trbovich A.M., Desanti A., Tilly K.I., Hirshfield A.N., Tilly J.L.
      Endocrinology 136:5042-5053(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 228-362.
      Tissue: Ovary.

    Entry informationi

    Entry nameiCASP1_RAT
    AccessioniPrimary (citable) accession number: P43527
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3