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Protein

Caspase-1

Gene

Casp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity).By similarity

Catalytic activityi

Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei236 – 2361By similarity
Active sitei284 – 2841By similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. scaffold protein binding Source: RGD

GO - Biological processi

  1. apoptotic process Source: RGD
  2. execution phase of apoptosis Source: GO_Central
  3. lung development Source: RGD
  4. memory Source: RGD
  5. microglial cell activation Source: RGD
  6. midgut development Source: RGD
  7. myoblast fusion Source: RGD
  8. positive regulation of apoptotic process Source: RGD
  9. positive regulation of circadian sleep/wake cycle, non-REM sleep Source: RGD
  10. positive regulation of cytokine secretion Source: RGD
  11. positive regulation of interleukin-1 beta secretion Source: RGD
  12. regulation of inflammatory response Source: GO_Central
  13. response to drug Source: RGD
  14. response to hypoxia Source: RGD
  15. response to lipopolysaccharide Source: RGD
  16. response to organic cyclic compound Source: RGD
  17. response to toxic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BRENDAi3.4.22.36. 5301.

Protein family/group databases

MEROPSiC14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-1 (EC:3.4.22.36)
Short name:
CASP-1
Alternative name(s):
Interleukin-1 beta convertase
Short name:
IL-1BC
Interleukin-1 beta-converting enzyme
Short name:
ICE
Short name:
IL-1 beta-converting enzyme
p45
Cleaved into the following 2 chains:
Gene namesi
Name:Casp1
Synonyms:Il1bc, Il1bce
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2274. Casp1.

Subcellular locationi

GO - Cellular componenti

  1. AIM2 inflammasome complex Source: UniProtKB
  2. cytoplasm Source: RGD
  3. IPAF inflammasome complex Source: UniProtKB
  4. neuron projection Source: RGD
  5. NLRP1 inflammasome complex Source: UniProtKB
  6. NLRP3 inflammasome complex Source: UniProtKB
  7. nucleus Source: RGD
  8. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – ?118118Sequence AnalysisPRO_0000004533Add
BLAST
Chaini?119 – 296178Caspase-1 subunit p20PRO_0000004534Add
BLAST
Propeptidei297 – 31418Sequence AnalysisPRO_0000004535Add
BLAST
Chaini315 – 40288Caspase-1 subunit p10PRO_0000004536Add
BLAST

Post-translational modificationi

The two subunits are derived from the precursor sequence by a autocatalytic mechanism.

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP43527.
PRIDEiP43527.

Expressioni

Gene expression databases

GenevestigatoriP43527.

Interactioni

Subunit structurei

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed bya 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p20 and p10 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-29840N.
STRINGi10116.ENSRNOP00000009993.

Structurei

3D structure databases

ProteinModelPortaliP43527.
SMRiP43527. Positions 2-90, 127-296, 315-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9191CARDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated
Contains 1 CARD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG326166.
HOGENOMiHOG000234399.
HOVERGENiHBG076981.
InParanoidiP43527.
PhylomeDBiP43527.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProiIPR001315. CARD.
IPR029030. Caspase-like_dom.
IPR017350. Caspase_ICE-type.
IPR011029. DEATH-like_dom.
IPR011600. Pept_C14_caspase.
IPR001309. Pept_C14_ICE_p20.
IPR016129. Pept_C14_ICE_p20_AS.
IPR002138. Pept_C14_p10.
IPR015917. Pept_C14A_p45_core.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF00656. Peptidase_C14. 1 hit.
[Graphical view]
PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00114. CARD. 1 hit.
SM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKVLRAKR KQFINSVSVG TINGLLDELL EKRVLNQEEM DTIKLANITV
60 70 80 90 100
MEKARDLCDH VTKKGPRASQ MFITYICNED CYLAEILELQ SGPSAETVFV
110 120 130 140 150
TEDSKGGHPF SSETKEKLNK EGGAFPGPSG SLKFCPLEIA QKLWKENHSE
160 170 180 190 200
IYPIMKTPTR TRLALIICNT DFQHLSRRVG ADVDLREMKL LLQDLGYTVK
210 220 230 240 250
VKENLTALEM TKELKEFAAC PEHKTSDSTF LVFMSHGLQE GICGITYSNE
260 270 280 290 300
VADILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKDSVGN
310 320 330 340 350
SEEGFLTDAI FEDDGIKKAH IEKDFIAFCS STPDNVSWRH PVRGSLFIES
360 370 380 390 400
LIKHMKEYAW SCDLEDIFRK VRFSFEQPDS RLQMPTTERV TLTKRFYLFP

GH
Length:402
Mass (Da):45,576
Last modified:November 1, 1995 - v1
Checksum:iD2E8FA2BEA76FD40
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 2521A → G in AAC52259 (PubMed:7588240).Curated
Sequence conflicti252 – 2521A → G in AAB35431 (PubMed:7588240).Curated
Sequence conflicti256 – 2561K → E in AAC52259 (PubMed:7588240).Curated
Sequence conflicti256 – 2561K → E in AAB35431 (PubMed:7588240).Curated
Sequence conflicti260 – 2601I → L in AAC52259 (PubMed:7588240).Curated
Sequence conflicti260 – 2601I → L in AAB35431 (PubMed:7588240).Curated
Sequence conflicti262 – 2621Q → H in AAC52259 (PubMed:7588240).Curated
Sequence conflicti262 – 2621Q → H in AAB35431 (PubMed:7588240).Curated
Sequence conflicti343 – 3431R → Q in AAC52259 (PubMed:7588240).Curated
Sequence conflicti343 – 3431R → Q in AAB35431 (PubMed:7588240).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14647 mRNA. Translation: AAA85812.1.
U34621 mRNA. Translation: AAC52259.1.
S79676 mRNA. Translation: AAB35431.1.
UniGeneiRn.37508.

Genome annotation databases

UCSCiRGD:2274. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14647 mRNA. Translation: AAA85812.1.
U34621 mRNA. Translation: AAC52259.1.
S79676 mRNA. Translation: AAB35431.1.
UniGeneiRn.37508.

3D structure databases

ProteinModelPortaliP43527.
SMRiP43527. Positions 2-90, 127-296, 315-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29840N.
STRINGi10116.ENSRNOP00000009993.

Protein family/group databases

MEROPSiC14.001.

Proteomic databases

PaxDbiP43527.
PRIDEiP43527.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2274. rat.

Organism-specific databases

RGDi2274. Casp1.

Phylogenomic databases

eggNOGiNOG326166.
HOGENOMiHOG000234399.
HOVERGENiHBG076981.
InParanoidiP43527.
PhylomeDBiP43527.

Enzyme and pathway databases

BRENDAi3.4.22.36. 5301.

Miscellaneous databases

PROiP43527.

Gene expression databases

GenevestigatoriP43527.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProiIPR001315. CARD.
IPR029030. Caspase-like_dom.
IPR017350. Caspase_ICE-type.
IPR011029. DEATH-like_dom.
IPR011600. Pept_C14_caspase.
IPR001309. Pept_C14_ICE_p20.
IPR016129. Pept_C14_ICE_p20_AS.
IPR002138. Pept_C14_p10.
IPR015917. Pept_C14A_p45_core.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF00656. Peptidase_C14. 1 hit.
[Graphical view]
PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00114. CARD. 1 hit.
SM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, tissue expression and regulation of rat interleukin 1 beta converting enzyme."
    Keane K.M., Giegel D.A., Lipinski W.J., Callahan M.J., Shivers B.D.
    Cytokine 7:105-110(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "Interleukin-1 beta-converting enzyme-related proteases (IRPs) and mammalian cell death: dissociation of IRP-induced oligonucleosomal endonuclease activity from morphological apoptosis in granulosa cells of the ovarian follicle."
    Flaws J.A., Kugu K., Trbovich A.M., Desanti A., Tilly K.I., Hirshfield A.N., Tilly J.L.
    Endocrinology 136:5042-5053(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 228-362.
    Tissue: Ovary.

Entry informationi

Entry nameiCASP1_RAT
AccessioniPrimary (citable) accession number: P43527
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 4, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.