P43524 (CYAA_ANACY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate cyclase
EC=4.6.1.1

Alternative name(s):
ATP pyrophosphate-lyase
Adenylyl cyclase

Gene names

Name:

cya

Organism

Anabaena cylindrica

Taxonomic identifier

1165 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May function as a membrane-localized receptor protein.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Cellular thylakoid membrane; Multi-pass membrane protein.
Domain	The N-terminal half could sense signals such as pH or a change in membrane potential and regulates the catalytic activity of the C-terminal half.
Sequence similarities	Belongs to the adenylyl cyclase class-3 family. Contains 1 guanylate cyclase domain. Contains 1 HAMP domain.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Membrane Thylakoid
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Adenylate cyclase		PRO_0000195742
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 46	21	Helical; Potential
	Topological domain	47 – 203	157	Lumenal, thylakoid Potential
	Transmembrane	204 – 226	23	Helical; Potential
	Topological domain	227 – 502	276	Cytoplasmic Potential
	Domain	227 – 280	54	HAMP
	Domain	320 – 451	132	Guanylate cyclase
Sites
	Metal binding	325	1	Magnesium By similarity
	Metal binding	369	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43524 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C61023B37CD8C4E9	FASTA	502	55,294
	10 20 30 40 50 60 MGDFCRRVDC KAMKFFALRS SIRTQIMAST TLLILALIGA IVTVWAKSES TLYHQEKLND 70 80 90 100 110 120 AKILSKVLSS TYANEVSEEN WSQIRLNIDL LLRENEDFLY ALVSDSNQKN QIAASSPDEF 130 140 150 160 170 180 QNNYIPDIVS LSVTNAAINS SEKTHVVETF ILRDIYFADK LRAKRGEKVM EVSSDIQTLS 190 200 210 220 230 240 GRKLGKLRIG ISLRKVNLAV TNAVNQALVV GFAGLNIGWI CAYFLAQHLS DPVRRLQISV 250 260 270 280 290 300 AKIAGGDLQH RADIHSRADE IGALATSVNE MSAALQISFN KLKKTLDSFE RFVPNKFISV 310 320 330 340 350 360 IAPQGIENIE VGAASTRRMT ILFCDIRGYT SMSEAMEPIE IFRFLNDYLA CMGKAIDEAG 370 380 390 400 410 420 GFIDKYIGDA IMALFDDGNT DCALHAAILM QQALDKFNDE RSMQTGKTGL PRISVGIGIH 430 440 450 460 470 480 RGTVVMGTVG FTSRIDSTVI GDAVNVASRI EGLTKQYGCN ILITESVVRN LSCPESFSLR 490 500 LIDKSVKVKG KDEAISIYEV KA

References

[1]	"Molecular cloning of the cyanobacterial adenylate cyclase gene from the filamentous cyanobacterium Anabaena cylindrica." Katayama M., Wada Y., Ohmori M. J. Bacteriol. 177:3873-3878(1995) [PubMed: 7601856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM M-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D55650 Genomic DNA. Translation: BAA09511.1.
PIR	I39600.
3D structure databases
ProteinModelPortal	P43524.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR003660. HAMP_linker_domain. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 1 hit.
Pfam	PF00211. Guanylate_cyc. 1 hit. PF00672. HAMP. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. SM00304. HAMP. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 1 hit.
PROSITE	PS50125. GUANYLATE_CYCLASE_2. 1 hit. PS50885. HAMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYAA_ANACY

Accession

Primary (citable) accession number: P43524

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 21, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program