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Protein

Glutamine synthetase

Gene

glnA

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi131Magnesium 1By similarity1
Metal bindingi133Magnesium 2By similarity1
Binding sitei209ATPBy similarity1
Metal bindingi214Magnesium 2By similarity1
Metal bindingi221Magnesium 2By similarity1
Binding sitei266L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi270Magnesium 1; via pros nitrogenBy similarity1
Binding sitei274ATPBy similarity1
Binding sitei320L-glutamateBy similarity1
Binding sitei326L-glutamateBy similarity1
Binding sitei338ATPBy similarity1
Binding sitei338L-glutamateBy similarity1
Binding sitei343ATPBy similarity1
Metal bindingi356Magnesium 1By similarity1
Binding sitei358L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 274ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifieri1063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532561 – 467Glutamine synthetaseAdd BLAST467

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei396O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP43518

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexameric ring.By similarity

Structurei

3D structure databases

ProteinModelPortaliP43518
SMRiP43518
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 266L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F Bacteria
COG0174 LUCA

Family and domain databases

Gene3Di3.10.20.70, 1 hit
InterProiView protein in InterPro
IPR008147 Gln_synt_b-grasp
IPR036651 Gln_synt_N
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR008146 Gln_synth_cat_dom
IPR027303 Gln_synth_gly_rich_site
IPR004809 Gln_synth_I
IPR027302 Gln_synth_N_conserv_site
PfamiView protein in Pfam
PF00120 Gln-synt_C, 1 hit
PF03951 Gln-synt_N, 1 hit
SMARTiView protein in SMART
SM01230 Gln-synt_C, 1 hit
SUPFAMiSSF54368 SSF54368, 1 hit
SSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR00653 GlnA, 1 hit
PROSITEiView protein in PROSITE
PS00180 GLNA_1, 1 hit
PS00181 GLNA_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

P43518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVADALKL MKDEEVEYVD IRFTDPRGKL QHVTLVADLV DEDFFEEGFM
60 70 80 90 100
FDGSSIAGWK SIDQSDMKLI PDAGSVYIDP FYAEKTLCVH CNVVEPDTGE
110 120 130 140 150
AYSRDPRGAA VKAEAYLKAS GIGDVAYFGP EAEFFIFDDV RYSVTPAKVA
160 170 180 190 200
YQIDADAGAW NTDSEYEMGN LAHRAGHKGG YFPVNPIDEA QDLRGEMLST
210 220 230 240 250
MKRMGMKVDK HHHEVATCQH ELGLIFGGLT EQADNILKYK YVIHNVAGMH
260 270 280 290 300
GKTVTFMPKP MKGDNGSGMH VNMSIWKEQA LFAGDKYADL SQEALWFIGG
310 320 330 340 350
ILKQPSVNAL TNPATNSYKR LIPGFEAPVL RAYSARNRSG CVRIPWTESP
360 370 380 390 400
NAKRVEARFP DPSANPYLAF AALLMAGLDG IKNKIDPGPA SDKDLYDLPP
410 420 430 440 450
EELAAIPTVC GSLREALEEL EKDHDFLLAG DVFTKDQLEG YMALKWEEVY
460
AYEHTPHPVE YQMYYSC
Length:467
Mass (Da):51,964
Last modified:November 1, 1995 - v1
Checksum:iBA93A04250BFB978
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71659 Genomic DNA Translation: CAA50651.1
PIRiS33181

Similar proteinsi

Entry informationi

Entry nameiGLN1B_RHOSH
AccessioniPrimary (citable) accession number: P43518
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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