Reviewed, UniProtKB/Swiss-Prot P43504 (NIR_LEPMC)

Names and origin

Protein names

Recommended name:
    Nitrite reductase [NAD(P)H]
    EC=1.7.1.4

Gene names

Name:

NIIA

Organism

Leptosphaeria maculans (Blackleg fungus)

Taxonomic identifier

5022 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Leptosphaeriaceae › Leptosphaeria › Leptosphaeria maculans complex

Protein attributes

Sequence length	185 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Ammonium hydroxide + 3 NAD(P)(+) + H(2)O = nitrite + 3 NAD(P)H.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 2Fe-2S cluster per subunit By similarity. FAD By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subunit structure	Homodimer By similarity.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	2Fe-2S FAD Flavoprotein Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrite reductase [NAD(P)H] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›185	›185	Nitrite reductase [NAD(P)H]		PRO_0000199959
Regions
	Nucleotide binding	72 – 107	36	FAD Potential
Experimental info
	Non-terminal residue	185	1

Sequences

	Sequence		Length	Mass (Da)
	P43504-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 19D67F97D386E7F9	FASTA	185	20,756
	10 20 30 40 50 60 MAATLPLLTQ GIEPVSGESY SPPGERHVQA TWGSKDGNIS NTEWNDPEAN RLPEKVAELE 70 80 90 100 110 120 KKGELNNSHP RRRVVVVGLG MVGVAFIEKL MKYDIKRREY DIIVIGEEPH LAYNRVGLTS 130 140 150 160 170 180 FFQHRQVENL YLNPQEWYSS MPEDSLHYHL NTLVTEIDSE NKTVKTSSGQ AVSYDILVLA TGSSS

References

[1]	"The nitrate and nitrite reductase-encoding genes of Leptosphaeria maculans are closely linked and transcribed in the same direction." Williams R.S.B., Davis M.A., Howlett B.J. Gene 158:153-154(1995) [PubMed: 7789806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Isolate 19.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18793 Genomic DNA. Translation: AAA82739.1.
PIR	PC4032.
3D structure databases
HSSP	HSSP built from PDB template 1D7Y based on UniProtKB Q52437.
ModBase	Search...
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006066. Nir_Si_BS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PROSITE	PS00365. NIR_SIR. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIR_LEPMC

Accession

Primary (citable) accession number: P43504

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)