Reviewed, UniProtKB/Swiss-Prot P43503 (XYLC_PSEPU)

Names and origin

Protein names

Recommended name:
    Benzaldehyde dehydrogenase [NAD+]
    EC=1.2.1.28

Gene names

Name:

xylC

Encoded on

Plasmid TOL pWW0 Ref.1
Plasmid TOL pWW53 Ref.2

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Benzaldehyde + NAD(+) + H(2)O = benzoate + NADH.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
Biophysicochemical properties	pH dependence: Optimum pH is 9.0.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	benzaldehyde dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Benzaldehyde dehydrogenase [NAD+]		PRO_0000056591
Regions
	Nucleotide binding	232 – 237	6	NAD By similarity
Sites
	Active site	254	1	By similarity
	Active site	288	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43503-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 093CB3E9487AF384	FASTA	487	51,897
	10 20 30 40 50 60 MRETKEQPIW YGKVFSSNWV EARGGVANVV DPSNGDILGI TGVANGEDVD AAVNAAKRAQ 70 80 90 100 110 120 KEWAAIPFSE RAAIVRKAAE KLKEREYEFA DWNVRECGAI RPKGLWEAGI AYEQMHQAAG 130 140 150 160 170 180 LASLPNGTLF PSAVPGRMNL CQRVPVGVVG VIAPWNFPLF LAMRSVAPAL ALGNAVILKP 190 200 210 220 230 240 DLQTAVTGGA LIAEIFSDAG MPDGVLHVLP GGADVGESMV ANSGINMISF TGSTQVGRLI 250 260 270 280 290 300 GEKCGRMLKK VALELGGNNV HIVLPDADLE GAVSCAAWGT FLHQGQVCMA AGRHLVHRDV 310 320 330 340 350 360 AQQYAEKLAL RAKNLVVGDP NSDQVHLGPL INEKQVVRVH ALVESAQRAG AQVLAGGTYQ 370 380 390 400 410 420 DRYYQATVIM DVKPEMEVFK SEIFGPVAPI TVFDSIEEAI ELANCSEYGL AASIHTRALA 430 440 450 460 470 480 TGLDIAKRLN TGMVHINDQP INCEPHVPFG GMGASGSGGR FGGPASIEEF TQSQWISMVE KPANYPF

References

[1]

"Overlapping substrate specificities of benzaldehyde dehydrogenase (the xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida." Inoue J., Shaw J.P., Rekik M., Harayama S. J. Bacteriol. 177:1196-1201(1995) [PubMed: 7868591] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15.

[2]

"Comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in Acinetobacter calcoaceticus and from the TOL-plasmid-encoded toluene pathway in Pseudomonas putida. N-terminal amino acid sequences, amino acid compositions and immunological cross-reactions." Chalmers R.M., Keen J.N., Fewson C.A. Biochem. J. 273:99-107(1991) [PubMed: 1989592] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-53.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U15151 Genomic DNA. Translation: AAA66218.1. D63341 Genomic DNA. Translation: BAA09661.1.
PIR	T47107.
RefSeq	NP_542888.1.
3D structure databases
HSSP	HSSP built from PDB template 1BI9 based on UniProtKB Q63639.
ModBase	Search...
Genome annotation databases
GeneID	1218745.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-2948. MetaCyc:MON-2968.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLC_PSEPU

Accession

Primary (citable) accession number: P43503

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 4, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)