ID   CWP2_YEAST              Reviewed;          92 AA.
AC   P43497; D6VXJ1;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 159.
DE   RecName: Full=Cell wall protein CWP2;
DE   AltName: Full=Low pH resistance protein 1;
DE   Flags: Precursor;
GN   Name=CWP2; Synonyms=LPR1; OrderedLocusNames=YKL096W-A;
GN   ORFNames=YKL096BW, YKL444;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=4R3;
RA   Skrzypek M.S., Dickson R.C., Zingg N.S., Lester R.L., Shelling J.G.;
RT   "Sphingolipids and the LPR1 gene are necessary for maintaining the
RT   intracellular pH of Saccharomyces cerevisiae.";
RL   Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8256524; DOI=10.1002/yea.320091016;
RA   Pallier C., Valens M., Puzos V., Fukuhara H., Cheret G., Sor F.,
RA   Bolotin-Fukuhara M.;
RT   "DNA sequence analysis of a 17 kb fragment of yeast chromosome XI
RT   physically localizes the MRB1 gene and reveals eight new open reading
RT   frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases.";
RL   Yeast 9:1149-1155(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA   Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA   von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA   Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   PROTEIN SEQUENCE OF 21-59, GLYCOSYLATION, AND SUBCELLULAR LOCATION.
RX   PubMed=7768807; DOI=10.1128/jb.177.11.3104-3110.1995;
RA   van der Vaart J.M., Caro L.H.P., Chapman J.W., Klis F.M., Verrips C.T.;
RT   "Identification of three mannoproteins in the cell wall of Saccharomyces
RT   cerevisiae.";
RL   J. Bacteriol. 177:3104-3110(1995).
RN   [7]
RP   INDUCTION.
RX   PubMed=11292809; DOI=10.1128/jb.183.9.2881-2887.2001;
RA   Abramova N.E., Sertil O., Mehta S., Lowry C.V.;
RT   "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene
RT   expression in Saccharomyces cerevisiae.";
RL   J. Bacteriol. 183:2881-2887(2001).
RN   [8]
RP   SUBCELLULAR LOCATION.
RX   PubMed=14617149; DOI=10.1046/j.1365-2958.2003.03722.x;
RA   Frieman M.B., Cormack B.P.;
RT   "The omega-site sequence of glycosylphosphatidylinositol-anchored proteins
RT   in Saccharomyces cerevisiae can determine distribution between the membrane
RT   and the cell wall.";
RL   Mol. Microbiol. 50:883-896(2003).
RN   [9]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [10]
RP   SUBCELLULAR LOCATION.
RX   PubMed=16672383; DOI=10.1091/mbc.e05-08-0738;
RA   Smits G.J., Schenkman L.R., Brul S., Pringle J.R., Klis F.M.;
RT   "Role of cell cycle-regulated expression in the localized incorporation of
RT   cell wall proteins in yeast.";
RL   Mol. Biol. Cell 17:3267-3280(2006).
CC   -!- FUNCTION: Component of the cell wall.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-
CC       anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP)
CC       found in young daughter cells.
CC   -!- INDUCTION: Down-regulated during anaerobic growth.
CC       {ECO:0000269|PubMed:11292809}.
CC   -!- PTM: Extensively O-glycosylated. {ECO:0000269|PubMed:7768807}.
CC   -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC       reticulum and serves to target the protein to the cell surface. There,
CC       the glucosamine-inositol phospholipid moiety is cleaved off and the
CC       GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC       glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC   -!- PTM: Covalently linked to beta-1,3-glucan of the inner cell wall layer
CC       via an alkali-sensitive ester linkage between the gamma-carboxyl group
CC       of glutamic acids, arising from a specific glutamine within the
CC       PIR1/2/3 repeat, and hydroxyl groups of glucoses of beta-1,3-glucan
CC       chains. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Present with 1590000 molecules/cell in log phase SD
CC       medium. {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
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DR   EMBL; U02299; AAA58697.1; -; Genomic_DNA.
DR   EMBL; X71133; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; Z28096; CAA81935.1; -; Genomic_DNA.
DR   EMBL; Z28097; CAA81937.1; -; Genomic_DNA.
DR   EMBL; AY558351; AAS56677.1; -; Genomic_DNA.
DR   EMBL; BK006944; DAA09061.1; -; Genomic_DNA.
DR   PIR; S39089; S39089.
DR   RefSeq; NP_012826.1; NM_001180025.1.
DR   AlphaFoldDB; P43497; -.
DR   BioGRID; 34036; 104.
DR   IntAct; P43497; 1.
DR   MINT; P43497; -.
DR   STRING; 4932.YKL096W-A; -.
DR   MaxQB; P43497; -.
DR   PaxDb; 4932-YKL096W-A; -.
DR   PeptideAtlas; P43497; -.
DR   EnsemblFungi; YKL096W-A_mRNA; YKL096W-A; YKL096W-A.
DR   GeneID; 853765; -.
DR   KEGG; sce:YKL096W-A; -.
DR   AGR; SGD:S000001956; -.
DR   SGD; S000001956; CWP2.
DR   VEuPathDB; FungiDB:YKL096W-A; -.
DR   eggNOG; ENOG502RQ57; Eukaryota.
DR   HOGENOM; CLU_181669_0_0_1; -.
DR   InParanoid; P43497; -.
DR   BioCyc; YEAST:G3O-32069-MONOMER; -.
DR   BioGRID-ORCS; 853765; 5 hits in 10 CRISPR screens.
DR   ChiTaRS; CWP2; yeast.
DR   PRO; PR:P43497; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   RNAct; P43497; Protein.
DR   GO; GO:0005829; C:cytosol; HDA:SGD.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
DR   GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005199; F:structural constituent of cell wall; IDA:SGD.
DR   GO; GO:0031505; P:fungal-type cell wall organization; IDA:SGD.
DR   InterPro; IPR000420; Yeast_PIR.
DR   Pfam; PF00399; PIR; 1.
DR   PROSITE; PS00929; PIR_REPEAT_1; 1.
DR   PROSITE; PS50256; PIR_REPEAT_2; 1.
PE   1: Evidence at protein level;
KW   Cell wall; Direct protein sequencing; Glycoprotein; GPI-anchor;
KW   Lipoprotein; Membrane; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:7768807"
FT   CHAIN           21..71
FT                   /note="Cell wall protein CWP2"
FT                   /id="PRO_0000033252"
FT   PROPEP          72..92
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000033253"
FT   REPEAT          24..37
FT                   /note="PIR1/2/3"
FT   REGION          41..60
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            33
FT                   /note="Covalent attachment to cell wall glycan"
FT                   /evidence="ECO:0000250"
FT   LIPID           71
FT                   /note="GPI-anchor amidated asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   92 AA;  8911 MW;  6AF6CD4B9E6E1BC7 CRC64;
     MQFSTVASVA FVALANFVAA ESAAAISQIT DGQIQATTTA TTEATTTAAP SSTVETVSPS
     STETISQQTE NGAAKAAVGM GAGALAAAAM LL
//