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P43497

- CWP2_YEAST

UniProt

P43497 - CWP2_YEAST

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Protein

Cell wall protein CWP2

Gene

CWP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the cell wall.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Covalent attachment to cell wall glycanBy similarity

GO - Molecular functioni

  1. structural constituent of cell wall Source: SGD

GO - Biological processi

  1. fungal-type cell wall organization Source: SGD
  2. regulation of pH Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32069-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall protein CWP2
Alternative name(s):
Low pH resistance protein 1
Gene namesi
Name:CWP2
Synonyms:LPR1
Ordered Locus Names:YKL096W-A
ORF Names:YKL096BW, YKL444
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL096w-a.
SGDiS000001956. CWP2.

Subcellular locationi

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP) found in young daughter cells.

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 PublicationAdd
BLAST
Chaini21 – 7151Cell wall protein CWP2PRO_0000033252Add
BLAST
Propeptidei72 – 9221Removed in mature formSequence AnalysisPRO_0000033253Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi71 – 711GPI-anchor amidated asparagineSequence Analysis

Post-translational modificationi

Extensively O-glycosylated.1 Publication
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from a specific glutamine within the PIR1/2/3 repeat, and hydroxyl groups of glucoses of beta-1,3-glucan chains.By similarity

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP43497.
PaxDbiP43497.

Expressioni

Inductioni

Down-regulated during anaerobic growth.1 Publication

Gene expression databases

GenevestigatoriP43497.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP1P253761EBI-2044078,EBI-2357
ALO1P547831EBI-2044078,EBI-2519

Protein-protein interaction databases

BioGridi34036. 25 interactions.
IntActiP43497. 1 interaction.
MINTiMINT-2787923.
STRINGi4932.YKL096W-A.

Structurei

3D structure databases

ProteinModelPortaliP43497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati24 – 3714PIR1/2/3Add
BLAST

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.Curated
Contains 1 PIR1/2/3 repeat.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiP43497.
OMAiKERENHT.

Family and domain databases

InterProiIPR000420. Yeast_PIR.
[Graphical view]
PfamiPF00399. PIR. 1 hit.
[Graphical view]
PROSITEiPS00929. PIR_REPEAT_1. 1 hit.
PS50256. PIR_REPEAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43497-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQFSTVASVA FVALANFVAA ESAAAISQIT DGQIQATTTA TTEATTTAAP
60 70 80 90
SSTVETVSPS STETISQQTE NGAAKAAVGM GAGALAAAAM LL
Length:92
Mass (Da):8,911
Last modified:November 1, 1995 - v1
Checksum:i6AF6CD4B9E6E1BC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02299 Genomic DNA. Translation: AAA58697.1.
X71133 Genomic DNA. No translation available.
Z28096 Genomic DNA. Translation: CAA81935.1.
Z28097 Genomic DNA. Translation: CAA81937.1.
AY558351 Genomic DNA. Translation: AAS56677.1.
BK006944 Genomic DNA. Translation: DAA09061.1.
PIRiS39089.
RefSeqiNP_012826.1. NM_001180025.1.

Genome annotation databases

EnsemblFungiiYKL096W-A; YKL096W-A; YKL096W-A.
GeneIDi853765.
KEGGisce:YKL096W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02299 Genomic DNA. Translation: AAA58697.1 .
X71133 Genomic DNA. No translation available.
Z28096 Genomic DNA. Translation: CAA81935.1 .
Z28097 Genomic DNA. Translation: CAA81937.1 .
AY558351 Genomic DNA. Translation: AAS56677.1 .
BK006944 Genomic DNA. Translation: DAA09061.1 .
PIRi S39089.
RefSeqi NP_012826.1. NM_001180025.1.

3D structure databases

ProteinModelPortali P43497.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34036. 25 interactions.
IntActi P43497. 1 interaction.
MINTi MINT-2787923.
STRINGi 4932.YKL096W-A.

Proteomic databases

MaxQBi P43497.
PaxDbi P43497.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL096W-A ; YKL096W-A ; YKL096W-A .
GeneIDi 853765.
KEGGi sce:YKL096W-A.

Organism-specific databases

CYGDi YKL096w-a.
SGDi S000001956. CWP2.

Phylogenomic databases

InParanoidi P43497.
OMAi KERENHT.

Enzyme and pathway databases

BioCyci YEAST:G3O-32069-MONOMER.

Miscellaneous databases

NextBioi 974852.

Gene expression databases

Genevestigatori P43497.

Family and domain databases

InterProi IPR000420. Yeast_PIR.
[Graphical view ]
Pfami PF00399. PIR. 1 hit.
[Graphical view ]
PROSITEi PS00929. PIR_REPEAT_1. 1 hit.
PS50256. PIR_REPEAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sphingolipids and the LPR1 gene are necessary for maintaining the intracellular pH of Saccharomyces cerevisiae."
    Skrzypek M.S., Dickson R.C., Zingg N.S., Lester R.L., Shelling J.G.
    Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 4R3.
  2. "DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases."
    Pallier C., Valens M., Puzos V., Fukuhara H., Cheret G., Sor F., Bolotin-Fukuhara M.
    Yeast 9:1149-1155(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae."
    van der Vaart J.M., Caro L.H.P., Chapman J.W., Klis F.M., Verrips C.T.
    J. Bacteriol. 177:3104-3110(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-59, GLYCOSYLATION, SUBCELLULAR LOCATION.
  7. "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae."
    Abramova N.E., Sertil O., Mehta S., Lowry C.V.
    J. Bacteriol. 183:2881-2887(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "The omega-site sequence of glycosylphosphatidylinositol-anchored proteins in Saccharomyces cerevisiae can determine distribution between the membrane and the cell wall."
    Frieman M.B., Cormack B.P.
    Mol. Microbiol. 50:883-896(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Role of cell cycle-regulated expression in the localized incorporation of cell wall proteins in yeast."
    Smits G.J., Schenkman L.R., Brul S., Pringle J.R., Klis F.M.
    Mol. Biol. Cell 17:3267-3280(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCWP2_YEAST
AccessioniPrimary (citable) accession number: P43497
Secondary accession number(s): D6VXJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3