P43497 (CWP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell wall protein CWP2

Alternative name(s):
Low pH resistance protein 1

Gene names

Name:	CWP2
Synonyms:	LPR1
Ordered Locus Names:	YKL096W-A
ORF Names:	YKL096BW, YKL444

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the cell wall.
Subcellular location	Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP) found in young daughter cells. Ref.6 Ref.8 Ref.10
Induction	Down-regulated during anaerobic growth. Ref.7
Post-translational modification	Extensively O-glycosylated. Ref.6 The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer. Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from a specific glutamine within the PIR1/2/3 repeat, and hydroxyl groups of glucoses of beta-1,3-glucan chains By similarity.
Miscellaneous	Present with 1590000 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SRP1/TIP1 family. Contains 1 PIR1/2/3 repeat.

Ontologies

Keywords
Cellular component	Cell wall Membrane Secreted
Domain	Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	fungal-type cell wall organization Inferred from direct assay Ref.6. Source: SGD regulation of pH Inferred from genetic interaction PubMed 11126778. Source: SGD
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Inferred from direct assay PubMed 11956747. Source: SGD
Molecular_function	structural constituent of cell wall Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AGP1	P25376	1	EBI-2044078,EBI-2357
ALO1	P54783	1	EBI-2044078,EBI-2519

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.6
	Chain	21 – 71	51	Cell wall protein CWP2		PRO_0000033252
	Propeptide	72 – 92	21	Removed in mature form Potential		PRO_0000033253
Regions
	Repeat	24 – 37	14	PIR1/2/3
Sites
	Site	33	1	Covalent attachment to cell wall glycan By similarity
Amino acid modifications
	Lipidation	71	1	GPI-anchor amidated asparagine Potential

Sequences

	Sequence		Length	Mass (Da)
	P43497 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6AF6CD4B9E6E1BC7	FASTA	92	8,911
	10 20 30 40 50 60 MQFSTVASVA FVALANFVAA ESAAAISQIT DGQIQATTTA TTEATTTAAP SSTVETVSPS 70 80 90 STETISQQTE NGAAKAAVGM GAGALAAAAM LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sphingolipids and the LPR1 gene are necessary for maintaining the intracellular pH of Saccharomyces cerevisiae." Skrzypek M.S., Dickson R.C., Zingg N.S., Lester R.L., Shelling J.G. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 4R3.
[2]	"DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases." Pallier C., Valens M., Puzos V., Fukuhara H., Cheret G., Sor F., Bolotin-Fukuhara M. Yeast 9:1149-1155(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae." van der Vaart J.M., Caro L.H.P., Chapman J.W., Klis F.M., Verrips C.T. J. Bacteriol. 177:3104-3110(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-59, GLYCOSYLATION, SUBCELLULAR LOCATION.
[7]	"Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae." Abramova N.E., Sertil O., Mehta S., Lowry C.V. J. Bacteriol. 183:2881-2887(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[8]	"The omega-site sequence of glycosylphosphatidylinositol-anchored proteins in Saccharomyces cerevisiae can determine distribution between the membrane and the cell wall." Frieman M.B., Cormack B.P. Mol. Microbiol. 50:883-896(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Role of cell cycle-regulated expression in the localized incorporation of cell wall proteins in yeast." Smits G.J., Schenkman L.R., Brul S., Pringle J.R., Klis F.M. Mol. Biol. Cell 17:3267-3280(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U02299 Genomic DNA. Translation: AAA58697.1. X71133 Genomic DNA. No translation available. Z28096 Genomic DNA. Translation: CAA81935.1. Z28097 Genomic DNA. Translation: CAA81937.1. AY558351 Genomic DNA. Translation: AAS56677.1. BK006944 Genomic DNA. Translation: DAA09061.1.
PIR	S39089.
RefSeq	NP_012826.1. NM_001180025.1.
3D structure databases
ProteinModelPortal	P43497.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43497. 1 interaction.
MINT	MINT-2787923.
STRING	4932.YKL096W-A.
Proteomic databases
PaxDb	P43497.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL096W-A; YKL096W-A; YKL096W-A.
GeneID	853765.
KEGG	sce:YKL096W-A.
Organism-specific databases
CYGD	YKL096w-a.
SGD	S000001956. CWP2.
Phylogenomic databases
GeneTree	ENSGT00600000085187.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32069-MONOMER.
Gene expression databases
Genevestigator	P43497.
GermOnline	YKL096W-A. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000420. Yeast_PIR. [Graphical view]
Pfam	PF00399. PIR. 1 hit. [Graphical view]
PROSITE	PS00929. PIR_REPEAT_1. 1 hit. PS50256. PIR_REPEAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974852.

Entry information

Entry name

CWP2_YEAST

Accession

Primary (citable) accession number: P43497
Secondary accession number(s): D6VXJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program