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Protein

Cell wall protein CWP2

Gene

CWP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cell wall.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Covalent attachment to cell wall glycanBy similarity1

GO - Molecular functioni

  • structural constituent of cell wall Source: SGD

GO - Biological processi

  • fungal-type cell wall organization Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32069-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall protein CWP2
Alternative name(s):
Low pH resistance protein 1
Gene namesi
Name:CWP2
Synonyms:LPR1
Ordered Locus Names:YKL096W-A
ORF Names:YKL096BW, YKL444
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL096W-A.
SGDiS000001956. CWP2.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular region Source: UniProtKB-KW
  • fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 201 PublicationAdd BLAST20
ChainiPRO_000003325221 – 71Cell wall protein CWP2Add BLAST51
PropeptideiPRO_000003325372 – 92Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi71GPI-anchor amidated asparagineSequence analysis1

Post-translational modificationi

Extensively O-glycosylated.1 Publication
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from a specific glutamine within the PIR1/2/3 repeat, and hydroxyl groups of glucoses of beta-1,3-glucan chains.By similarity

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP43497.
PRIDEiP43497.

Expressioni

Inductioni

Down-regulated during anaerobic growth.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi34036. 24 interactors.
IntActiP43497. 1 interactor.
MINTiMINT-2787923.

Structurei

3D structure databases

ProteinModelPortaliP43497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati24 – 37PIR1/2/3Add BLAST14

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.Curated
Contains 1 PIR1/2/3 repeat.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiP43497.
OMAiMQFSVVA.

Family and domain databases

InterProiIPR000420. Yeast_PIR.
[Graphical view]
PfamiPF00399. PIR. 1 hit.
[Graphical view]
PROSITEiPS00929. PIR_REPEAT_1. 1 hit.
PS50256. PIR_REPEAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43497-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFSTVASVA FVALANFVAA ESAAAISQIT DGQIQATTTA TTEATTTAAP
60 70 80 90
SSTVETVSPS STETISQQTE NGAAKAAVGM GAGALAAAAM LL
Length:92
Mass (Da):8,911
Last modified:November 1, 1995 - v1
Checksum:i6AF6CD4B9E6E1BC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02299 Genomic DNA. Translation: AAA58697.1.
X71133 Genomic DNA. No translation available.
Z28096 Genomic DNA. Translation: CAA81935.1.
Z28097 Genomic DNA. Translation: CAA81937.1.
AY558351 Genomic DNA. Translation: AAS56677.1.
BK006944 Genomic DNA. Translation: DAA09061.1.
PIRiS39089.
RefSeqiNP_012826.1. NM_001180025.1.

Genome annotation databases

EnsemblFungiiYKL096W-A; YKL096W-A; YKL096W-A.
GeneIDi853765.
KEGGisce:YKL096W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02299 Genomic DNA. Translation: AAA58697.1.
X71133 Genomic DNA. No translation available.
Z28096 Genomic DNA. Translation: CAA81935.1.
Z28097 Genomic DNA. Translation: CAA81937.1.
AY558351 Genomic DNA. Translation: AAS56677.1.
BK006944 Genomic DNA. Translation: DAA09061.1.
PIRiS39089.
RefSeqiNP_012826.1. NM_001180025.1.

3D structure databases

ProteinModelPortaliP43497.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34036. 24 interactors.
IntActiP43497. 1 interactor.
MINTiMINT-2787923.

Proteomic databases

MaxQBiP43497.
PRIDEiP43497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL096W-A; YKL096W-A; YKL096W-A.
GeneIDi853765.
KEGGisce:YKL096W-A.

Organism-specific databases

EuPathDBiFungiDB:YKL096W-A.
SGDiS000001956. CWP2.

Phylogenomic databases

InParanoidiP43497.
OMAiMQFSVVA.

Enzyme and pathway databases

BioCyciYEAST:G3O-32069-MONOMER.

Miscellaneous databases

PROiP43497.

Family and domain databases

InterProiIPR000420. Yeast_PIR.
[Graphical view]
PfamiPF00399. PIR. 1 hit.
[Graphical view]
PROSITEiPS00929. PIR_REPEAT_1. 1 hit.
PS50256. PIR_REPEAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCWP2_YEAST
AccessioniPrimary (citable) accession number: P43497
Secondary accession number(s): D6VXJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 5, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.