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P43497

- CWP2_YEAST

UniProt

P43497 - CWP2_YEAST

Protein

Cell wall protein CWP2

Gene

CWP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the cell wall.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei33 – 331Covalent attachment to cell wall glycanBy similarity

    GO - Molecular functioni

    1. structural constituent of cell wall Source: SGD

    GO - Biological processi

    1. fungal-type cell wall organization Source: SGD
    2. regulation of pH Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32069-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell wall protein CWP2
    Alternative name(s):
    Low pH resistance protein 1
    Gene namesi
    Name:CWP2
    Synonyms:LPR1
    Ordered Locus Names:YKL096W-A
    ORF Names:YKL096BW, YKL444
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL096w-a.
    SGDiS000001956. CWP2.

    Subcellular locationi

    Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
    Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP) found in young daughter cells.

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: UniProtKB-KW
    3. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Cell wall, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 20201 PublicationAdd
    BLAST
    Chaini21 – 7151Cell wall protein CWP2PRO_0000033252Add
    BLAST
    Propeptidei72 – 9221Removed in mature formSequence AnalysisPRO_0000033253Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi71 – 711GPI-anchor amidated asparagineSequence Analysis

    Post-translational modificationi

    Extensively O-glycosylated.1 Publication
    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
    Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from a specific glutamine within the PIR1/2/3 repeat, and hydroxyl groups of glucoses of beta-1,3-glucan chains.By similarity

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiP43497.
    PaxDbiP43497.

    Expressioni

    Inductioni

    Down-regulated during anaerobic growth.1 Publication

    Gene expression databases

    GenevestigatoriP43497.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AGP1P253761EBI-2044078,EBI-2357
    ALO1P547831EBI-2044078,EBI-2519

    Protein-protein interaction databases

    BioGridi34036. 24 interactions.
    IntActiP43497. 1 interaction.
    MINTiMINT-2787923.
    STRINGi4932.YKL096W-A.

    Structurei

    3D structure databases

    ProteinModelPortaliP43497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati24 – 3714PIR1/2/3Add
    BLAST

    Sequence similaritiesi

    Belongs to the SRP1/TIP1 family.Curated
    Contains 1 PIR1/2/3 repeat.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    OMAiKERENHT.

    Family and domain databases

    InterProiIPR000420. Yeast_PIR.
    [Graphical view]
    PfamiPF00399. PIR. 1 hit.
    [Graphical view]
    PROSITEiPS00929. PIR_REPEAT_1. 1 hit.
    PS50256. PIR_REPEAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43497-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQFSTVASVA FVALANFVAA ESAAAISQIT DGQIQATTTA TTEATTTAAP   50
    SSTVETVSPS STETISQQTE NGAAKAAVGM GAGALAAAAM LL 92
    Length:92
    Mass (Da):8,911
    Last modified:November 1, 1995 - v1
    Checksum:i6AF6CD4B9E6E1BC7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02299 Genomic DNA. Translation: AAA58697.1.
    X71133 Genomic DNA. No translation available.
    Z28096 Genomic DNA. Translation: CAA81935.1.
    Z28097 Genomic DNA. Translation: CAA81937.1.
    AY558351 Genomic DNA. Translation: AAS56677.1.
    BK006944 Genomic DNA. Translation: DAA09061.1.
    PIRiS39089.
    RefSeqiNP_012826.1. NM_001180025.1.

    Genome annotation databases

    EnsemblFungiiYKL096W-A; YKL096W-A; YKL096W-A.
    GeneIDi853765.
    KEGGisce:YKL096W-A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02299 Genomic DNA. Translation: AAA58697.1 .
    X71133 Genomic DNA. No translation available.
    Z28096 Genomic DNA. Translation: CAA81935.1 .
    Z28097 Genomic DNA. Translation: CAA81937.1 .
    AY558351 Genomic DNA. Translation: AAS56677.1 .
    BK006944 Genomic DNA. Translation: DAA09061.1 .
    PIRi S39089.
    RefSeqi NP_012826.1. NM_001180025.1.

    3D structure databases

    ProteinModelPortali P43497.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34036. 24 interactions.
    IntActi P43497. 1 interaction.
    MINTi MINT-2787923.
    STRINGi 4932.YKL096W-A.

    Proteomic databases

    MaxQBi P43497.
    PaxDbi P43497.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL096W-A ; YKL096W-A ; YKL096W-A .
    GeneIDi 853765.
    KEGGi sce:YKL096W-A.

    Organism-specific databases

    CYGDi YKL096w-a.
    SGDi S000001956. CWP2.

    Phylogenomic databases

    OMAi KERENHT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32069-MONOMER.

    Miscellaneous databases

    NextBioi 974852.

    Gene expression databases

    Genevestigatori P43497.

    Family and domain databases

    InterProi IPR000420. Yeast_PIR.
    [Graphical view ]
    Pfami PF00399. PIR. 1 hit.
    [Graphical view ]
    PROSITEi PS00929. PIR_REPEAT_1. 1 hit.
    PS50256. PIR_REPEAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sphingolipids and the LPR1 gene are necessary for maintaining the intracellular pH of Saccharomyces cerevisiae."
      Skrzypek M.S., Dickson R.C., Zingg N.S., Lester R.L., Shelling J.G.
      Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 4R3.
    2. "DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases."
      Pallier C., Valens M., Puzos V., Fukuhara H., Cheret G., Sor F., Bolotin-Fukuhara M.
      Yeast 9:1149-1155(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae."
      van der Vaart J.M., Caro L.H.P., Chapman J.W., Klis F.M., Verrips C.T.
      J. Bacteriol. 177:3104-3110(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-59, GLYCOSYLATION, SUBCELLULAR LOCATION.
    7. "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae."
      Abramova N.E., Sertil O., Mehta S., Lowry C.V.
      J. Bacteriol. 183:2881-2887(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "The omega-site sequence of glycosylphosphatidylinositol-anchored proteins in Saccharomyces cerevisiae can determine distribution between the membrane and the cell wall."
      Frieman M.B., Cormack B.P.
      Mol. Microbiol. 50:883-896(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Role of cell cycle-regulated expression in the localized incorporation of cell wall proteins in yeast."
      Smits G.J., Schenkman L.R., Brul S., Pringle J.R., Klis F.M.
      Mol. Biol. Cell 17:3267-3280(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCWP2_YEAST
    AccessioniPrimary (citable) accession number: P43497
    Secondary accession number(s): D6VXJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1590000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3