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P43497 (CWP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell wall protein CWP2
Alternative name(s):
Low pH resistance protein 1
Gene names
Name:CWP2
Synonyms:LPR1
Ordered Locus Names:YKL096W-A
ORF Names:YKL096BW, YKL444
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length92 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cell wall.

Subcellular location

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP) found in young daughter cells. Ref.6 Ref.8 Ref.10

Induction

Down-regulated during anaerobic growth. Ref.7

Post-translational modification

Extensively O-glycosylated. Ref.6

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from a specific glutamine within the PIR1/2/3 repeat, and hydroxyl groups of glucoses of beta-1,3-glucan chains By similarity.

Miscellaneous

Present with 1590000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SRP1/TIP1 family.

Contains 1 PIR1/2/3 repeat.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AGP1P253761EBI-2044078,EBI-2357
ALO1P547831EBI-2044078,EBI-2519

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.6
Chain21 – 7151Cell wall protein CWP2
PRO_0000033252
Propeptide72 – 9221Removed in mature form Potential
PRO_0000033253

Regions

Repeat24 – 3714PIR1/2/3

Sites

Site331Covalent attachment to cell wall glycan By similarity

Amino acid modifications

Lipidation711GPI-anchor amidated asparagine Potential

Sequences

Sequence LengthMass (Da)Tools
P43497 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 6AF6CD4B9E6E1BC7

FASTA928,911
        10         20         30         40         50         60 
MQFSTVASVA FVALANFVAA ESAAAISQIT DGQIQATTTA TTEATTTAAP SSTVETVSPS 

        70         80         90 
STETISQQTE NGAAKAAVGM GAGALAAAAM LL 

« Hide

References

« Hide 'large scale' references
[1]"Sphingolipids and the LPR1 gene are necessary for maintaining the intracellular pH of Saccharomyces cerevisiae."
Skrzypek M.S., Dickson R.C., Zingg N.S., Lester R.L., Shelling J.G.
Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 4R3.
[2]"DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases."
Pallier C., Valens M., Puzos V., Fukuhara H., Cheret G., Sor F., Bolotin-Fukuhara M.
Yeast 9:1149-1155(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae."
van der Vaart J.M., Caro L.H.P., Chapman J.W., Klis F.M., Verrips C.T.
J. Bacteriol. 177:3104-3110(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-59, GLYCOSYLATION, SUBCELLULAR LOCATION.
[7]"Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae."
Abramova N.E., Sertil O., Mehta S., Lowry C.V.
J. Bacteriol. 183:2881-2887(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[8]"The omega-site sequence of glycosylphosphatidylinositol-anchored proteins in Saccharomyces cerevisiae can determine distribution between the membrane and the cell wall."
Frieman M.B., Cormack B.P.
Mol. Microbiol. 50:883-896(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Role of cell cycle-regulated expression in the localized incorporation of cell wall proteins in yeast."
Smits G.J., Schenkman L.R., Brul S., Pringle J.R., Klis F.M.
Mol. Biol. Cell 17:3267-3280(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U02299 Genomic DNA. Translation: AAA58697.1.
X71133 Genomic DNA. No translation available.
Z28096 Genomic DNA. Translation: CAA81935.1.
Z28097 Genomic DNA. Translation: CAA81937.1.
AY558351 Genomic DNA. Translation: AAS56677.1.
BK006944 Genomic DNA. Translation: DAA09061.1.
PIRS39089.
RefSeqNP_012826.1. NM_001180025.1.

3D structure databases

ProteinModelPortalP43497.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34036. 24 interactions.
IntActP43497. 1 interaction.
MINTMINT-2787923.
STRING4932.YKL096W-A.

Proteomic databases

MaxQBP43497.
PaxDbP43497.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL096W-A; YKL096W-A; YKL096W-A.
GeneID853765.
KEGGsce:YKL096W-A.

Organism-specific databases

CYGDYKL096w-a.
SGDS000001956. CWP2.

Phylogenomic databases

OMAKERENHT.

Enzyme and pathway databases

BioCycYEAST:G3O-32069-MONOMER.

Gene expression databases

GenevestigatorP43497.

Family and domain databases

InterProIPR000420. Yeast_PIR.
[Graphical view]
PfamPF00399. PIR. 1 hit.
[Graphical view]
PROSITEPS00929. PIR_REPEAT_1. 1 hit.
PS50256. PIR_REPEAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974852.

Entry information

Entry nameCWP2_YEAST
AccessionPrimary (citable) accession number: P43497
Secondary accession number(s): D6VXJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families