Reviewed, UniProtKB/Swiss-Prot P43496 (TRXB_PENCH)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
    EC=1.8.1.9

Gene names

Name:	TRR1
Synonyms:	TRXB

Organism

Penicillium chrysogenum (Penicillium notatum)

Taxonomic identifier

5076 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium › Penicillium chrysogenum complex

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the thioredoxin-thioredoxin reductase system which may be involved in biosynthesis of penicillins and cephalosporins and may be important in determining the thiol-disulfide redox balance.
Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 334	333	Thioredoxin reductase		PRO_0000166765
Regions
	Nucleotide binding	33 – 45	13	FAD By similarity
	Nucleotide binding	294 – 303	10	FAD By similarity
	Compositional bias	47 – 50	4	Poly-Thr
Amino acid modifications
	Disulfide bond	145 ↔ 148		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43496-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 7A3A7457D7486341	FASTA	334	35,701
	10 20 30 40 50 60 MVHSKVVIIG SGAGAHTAAI YLSRAELQPV LYEGMLANGT AAGGQLTTTT DVENFPGFPS 70 80 90 100 110 120 GIGGAELMDN MRAQSERFGT EIITETISKL DLSSRPFKMW TEWNDDEGSE PVRTADAVII 130 140 150 160 170 180 ATGANARRLN LPGEETYWQN GISACAVCDG AVPIFRNKPL YVIGGGDSAA EEAMFLAKYG 190 200 210 220 230 240 SSVTVLVRKD KLRASNIMAD RLLAHPKCKV RFNTVATEVI GENKPNGLMT HLRVKDVLSN 250 260 270 280 290 300 AEEVVEANGL FYAVGHDPAS GLVKGQVELD DEGYIITKPG TSFTNVEGVF ACGDVQDKRY 310 320 330 RQAITSAGSG CVAALEAEKF IAETETHQEA KPVL

References

[1]

"The thioredoxin system of Penicillium chrysogenum and its possible role in penicillin biosynthesis." Cohen G., Argaman A., Schreiber R., Mislovati M., Aharonowitz Y. J. Bacteriol. 176:973-984(1994) [PubMed: 8106340] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-29. Strain: ATCC 9480 / CBS 307.48 / NRRL 1951 / GB8 / QM 941.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76119 Genomic DNA. Translation: CAA53725.1.
PIR	B49888.
3D structure databases
HSSP	HSSP built from PDB template 1VDC based on UniProtKB Q39243.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.8.1.9. 285.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_PENCH

Accession

Primary (citable) accession number: P43496

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)