P43494 (THCD_RHOER) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhodocoxin reductase
EC=1.18.1.-

Gene names

Name:

thcD

Organism

Rhodococcus erythropolis (Arthrobacter picolinophilus)

Taxonomic identifier

1833 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation.
Cofactor	FAD.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 427	426	Rhodocoxin reductase		PRO_0000167647
Regions
	Nucleotide binding	2 – 34	33	FAD Potential
	Nucleotide binding	144 – 172	29	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P43494 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 089253050B7A1F92	FASTA	427	45,270
	10 20 30 40 50 60 MSIVIIGSGQ AGFEAAVSLR SHGFSGTITL VGDEPGVPYQ RPPLSKAYLH SDPDRESLAL 70 80 90 100 110 120 RPAQYFDDHR ITLTCGKPVV RIDRDAQRVE LIDATAIEYD HLILATGARN RLLPVPGANL 130 140 150 160 170 180 PGVHYLRTAG EAESLTSSMA SCSSLVVIGA GFIGLEVAAA ARKKGLDVTV VEAMDRPMAR 190 200 210 220 230 240 ALSSVMSGYF STAHTEHGVH MRLSTGVKTI NAADGRAAGV TTNSGDVIHA DAVVVGIGVV 250 260 270 280 290 300 PNIELAALTG LPVDNGIVVD EYLRTPDENI SAIGDCAAYP IPGKAGLVRL ESVQNAVDQA 310 320 330 340 350 360 RCLAAQLTGT STHYRSVPWF WSEQYESKLQ MAGLTAGADT HVVRGSVDSG VFSIFCFLGT 370 380 390 400 410 420 RLLGVESVNK PRDHMAARKI LATEMPLTPE QAADTDFDLK LAIARHKDTH KDEVASADIG ERQVVAS

References

[1]

"Degradation of the thiocarbamate herbicide EPTC (S-ethyl dipropylcarbamothioate) and biosafening by Rhodococcus sp. strain NI86/21 involve an inducible cytochrome P-450 system and aldehyde dehydrogenase." Nagy I., Schoofs G., Compernolle F., Proost P., Vanderleyden J., de Mot R. J. Bacteriol. 177:676-687(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11. Strain: NI86/21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17130 Genomic DNA. Translation: AAC45752.1.
3D structure databases
ProteinModelPortal	P43494.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.390.30. 1 hit.
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 2 hits. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THCD_RHOER

Accession

Primary (citable) accession number: P43494

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program