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Protein

Tumor necrosis factor ligand superfamily member 4

Gene

Tnfsf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.

GO - Molecular functioni

  • tumor necrosis factor receptor binding Source: MGI
  • tumor necrosis factor receptor superfamily binding Source: BHF-UCL

GO - Biological processi

  • acute inflammatory response Source: BHF-UCL
  • blood vessel development Source: BHF-UCL
  • CD4-positive, alpha-beta T cell costimulation Source: BHF-UCL
  • cellular response to lipopolysaccharide Source: BHF-UCL
  • cellular response to nitrogen dioxide Source: BHF-UCL
  • cellular response to prostaglandin E stimulus Source: BHF-UCL
  • chemokine (C-C motif) ligand 11 production Source: BHF-UCL
  • cholesterol metabolic process Source: MGI
  • defense response to nematode Source: BHF-UCL
  • inflammatory response Source: MGI
  • innate immune response Source: BHF-UCL
  • memory T cell activation Source: BHF-UCL
  • negative regulation of activation-induced cell death of T cells Source: BHF-UCL
  • negative regulation of cytokine secretion Source: MGI
  • negative regulation of extrinsic apoptotic signaling pathway Source: BHF-UCL
  • negative regulation of interferon-gamma production Source: BHF-UCL
  • negative regulation of interleukin-17 production Source: BHF-UCL
  • negative regulation of regulatory T cell differentiation Source: BHF-UCL
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  • negative regulation of T cell apoptotic process Source: BHF-UCL
  • negative regulation of T-helper 1 cell differentiation Source: BHF-UCL
  • negative regulation of transcription, DNA-templated Source: BHF-UCL
  • positive regulation of activated T cell proliferation Source: Ensembl
  • positive regulation of alpha-beta T cell proliferation Source: BHF-UCL
  • positive regulation of B cell activation Source: BHF-UCL
  • positive regulation of CD4-positive, alpha-beta T cell costimulation Source: BHF-UCL
  • positive regulation of CD4-positive, alpha-beta T cell differentiation Source: BHF-UCL
  • positive regulation of immune effector process Source: BHF-UCL
  • positive regulation of immunoglobulin mediated immune response Source: BHF-UCL
  • positive regulation of immunoglobulin secretion Source: BHF-UCL
  • positive regulation of inflammatory response Source: BHF-UCL
  • positive regulation of interferon-gamma production Source: BHF-UCL
  • positive regulation of interleukin-10 production Source: BHF-UCL
  • positive regulation of interleukin-12 production Source: BHF-UCL
  • positive regulation of interleukin-13 production Source: BHF-UCL
  • positive regulation of interleukin-2 production Source: Ensembl
  • positive regulation of interleukin-4-dependent isotype switching to IgE isotypes Source: BHF-UCL
  • positive regulation of interleukin-4 production Source: BHF-UCL
  • positive regulation of interleukin-6 production Source: BHF-UCL
  • positive regulation of memory T cell activation Source: BHF-UCL
  • positive regulation of memory T cell differentiation Source: BHF-UCL
  • positive regulation of T cell costimulation Source: BHF-UCL
  • positive regulation of T cell cytokine production Source: BHF-UCL
  • positive regulation of T cell migration Source: BHF-UCL
  • positive regulation of T-helper 2 cell activation Source: BHF-UCL
  • positive regulation of T-helper 2 cell differentiation Source: BHF-UCL
  • positive regulation of type 2 immune response Source: BHF-UCL
  • regulation of adaptive immune response Source: BHF-UCL
  • regulation of inflammatory response Source: BHF-UCL
  • response to nitrogen dioxide Source: BHF-UCL
  • response to virus Source: BHF-UCL
  • T cell proliferation Source: Ensembl
  • T-helper 2 cell activation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor necrosis factor ligand superfamily member 4
Alternative name(s):
OX40 ligand
Short name:
OX40L
CD_antigen: CD252
Gene namesi
Name:Tnfsf4
Synonyms:Ox40l, Txgp1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:104511. Tnfsf4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei29 – 5022Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini51 – 198148ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198Tumor necrosis factor ligand superfamily member 4PRO_0000185494Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 1631 Publication
Glycosylationi91 – 911N-linked (GlcNAc...)1 Publication
Disulfide bondi98 ↔ 1831 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP43488.

PTM databases

PhosphoSiteiP43488.

Expressioni

Gene expression databases

BgeeiP43488.
ExpressionAtlasiP43488. baseline and differential.
GenevisibleiP43488. MM.

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

DIPiDIP-6238N.
IntActiP43488. 1 interaction.
STRINGi10090.ENSMUSP00000028024.

Structurei

Secondary structure

1
198
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi62 – 7110Combined sources
Beta strandi74 – 785Combined sources
Beta strandi81 – 833Combined sources
Beta strandi87 – 904Combined sources
Beta strandi93 – 953Combined sources
Beta strandi100 – 11213Combined sources
Beta strandi116 – 1194Combined sources
Beta strandi127 – 1293Combined sources
Beta strandi135 – 14612Combined sources
Beta strandi151 – 1566Combined sources
Helixi160 – 1656Combined sources
Beta strandi168 – 17710Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HEWX-ray1.45F51-198[»]
2HEYX-ray2.00F/G51-198[»]
ProteinModelPortaliP43488.
SMRiP43488. Positions 57-191.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43488.

Family & Domainsi

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47572.
GeneTreeiENSGT00390000015127.
HOGENOMiHOG000076309.
HOVERGENiHBG056457.
InParanoidiP43488.
KOiK05469.
OMAiMEGVQPL.
OrthoDBiEOG74BJVD.
PhylomeDBiP43488.
TreeFamiTF336384.

Family and domain databases

InterProiIPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGEGVQPLD ENLENGSRPR FKWKKTLRLV VSGIKGAGML LCFIYVCLQL
60 70 80 90 100
SSSPAKDPPI QRLRGAVTRC EDGQLFISSY KNEYQTMEVQ NNSVVIKCDG
110 120 130 140 150
LYIIYLKGSF FQEVKIDLHF REDHNPISIP MLNDGRRIVF TVVASLAFKD
160 170 180 190
KVYLTVNAPD TLCEHLQIND GELIVVQLTP GYCAPEGSYH STVNQVPL
Length:198
Mass (Da):22,255
Last modified:November 1, 1995 - v1
Checksum:iF4B23A7CC0590459
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12763 mRNA. Translation: AAA21871.1.
CCDSiCCDS15416.1.
PIRiS48290.
RefSeqiNP_033478.1. NM_009452.2.
UniGeneiMm.4994.

Genome annotation databases

EnsembliENSMUST00000028024; ENSMUSP00000028024; ENSMUSG00000026700.
GeneIDi22164.
KEGGimmu:22164.
UCSCiuc007dfn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12763 mRNA. Translation: AAA21871.1.
CCDSiCCDS15416.1.
PIRiS48290.
RefSeqiNP_033478.1. NM_009452.2.
UniGeneiMm.4994.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HEWX-ray1.45F51-198[»]
2HEYX-ray2.00F/G51-198[»]
ProteinModelPortaliP43488.
SMRiP43488. Positions 57-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6238N.
IntActiP43488. 1 interaction.
STRINGi10090.ENSMUSP00000028024.

PTM databases

PhosphoSiteiP43488.

Proteomic databases

PRIDEiP43488.

Protocols and materials databases

DNASUi22164.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028024; ENSMUSP00000028024; ENSMUSG00000026700.
GeneIDi22164.
KEGGimmu:22164.
UCSCiuc007dfn.2. mouse.

Organism-specific databases

CTDi7292.
MGIiMGI:104511. Tnfsf4.

Phylogenomic databases

eggNOGiNOG47572.
GeneTreeiENSGT00390000015127.
HOGENOMiHOG000076309.
HOVERGENiHBG056457.
InParanoidiP43488.
KOiK05469.
OMAiMEGVQPL.
OrthoDBiEOG74BJVD.
PhylomeDBiP43488.
TreeFamiTF336384.

Miscellaneous databases

EvolutionaryTraceiP43488.
NextBioi302098.
PROiP43488.
SOURCEiSearch...

Gene expression databases

BgeeiP43488.
ExpressionAtlasiP43488. baseline and differential.
GenevisibleiP43488. MM.

Family and domain databases

InterProiIPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of murine and human OX40/OX40 ligand systems: identification of a human OX40 ligand as the HTLV-1-regulated protein gp34."
    Baum P.R., Gayle R.B. III, Ramsdell F., Srinivasan S., Sorensen R.A., Watson M.L., Seldin M.F., Baker E., Sutherland G.R., Clifford K.N., Alderson M.R., Goodwin R.G., Fanslow W.C.
    EMBO J. 13:3992-4001(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The crystal structure of the costimulatory OX40-OX40L complex."
    Compaan D.M., Hymowitz S.G.
    Structure 14:1321-1330(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 50-198, DISULFIDE BONDS, SUBUNIT, GLYCOSYLATION AT ASN-91.

Entry informationi

Entry nameiTNFL4_MOUSE
AccessioniPrimary (citable) accession number: P43488
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.