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Protein

Ran-specific GTPase-activating protein

Gene

RANBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed:20485264). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PubMed:17671426). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed:7882974). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PubMed:7882974, PubMed:7616957).By similarity4 Publications

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • GDP-dissociation inhibitor activity Source: GO_Central
  • GTPase activator activity Source: UniProtKB-KW
  • Ran GTPase binding Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-165054. Rev-mediated nuclear export of HIV RNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Ran-specific GTPase-activating protein
Alternative name(s):
Ran-binding protein 1
Short name:
RanBP1
Gene namesi
Name:RANBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000099901.16.
HGNCiHGNC:9847. RANBP1.
MIMi601180. gene.
neXtProtiNX_P43487.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi5902.
OpenTargetsiENSG00000099901.
PharmGKBiPA34206.

Polymorphism and mutation databases

BioMutaiRANBP1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002136672 – 201Ran-specific GTPase-activating proteinAdd BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei13PhosphothreonineCombined sources1
Modified residuei18PhosphothreonineCombined sources1
Modified residuei21PhosphoserineCombined sources1
Modified residuei60PhosphoserineCombined sources1
Modified residuei150N6-acetyllysine; alternateCombined sources1
Modified residuei150N6-succinyllysine; alternateBy similarity1
Modified residuei183N6-acetyllysineCombined sources1
Modified residuei188PhosphoserineCombined sources1
Cross-linki190Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP43487.
MaxQBiP43487.
PaxDbiP43487.
PeptideAtlasiP43487.
PRIDEiP43487.
TopDownProteomicsiP43487-1. [P43487-1]

2D gel databases

DOSAC-COBS-2DPAGEiP43487.
OGPiP43487.
REPRODUCTION-2DPAGEiIPI00414127.

PTM databases

iPTMnetiP43487.
PhosphoSitePlusiP43487.
SwissPalmiP43487.

Expressioni

Gene expression databases

BgeeiENSG00000099901.
CleanExiHS_RANBP1.
ExpressionAtlasiP43487. baseline and differential.
GenevisibleiP43487. HS.

Organism-specific databases

HPAiCAB046463.
HPA065868.
HPA065931.

Interactioni

Subunit structurei

Interacts with RAN (via C-terminus of GTP-bound form) but not with GDP-bound RAN (PubMed:7882974, PubMed:7891706, PubMed:8896452). Identified in a complex composed of RAN, RANGAP1 and RANBP1 (PubMed:16428860, PubMed:11832950). Identified in a complex that contains TNPO1, RAN and RANBP1. Identified in a complex that contains CSE1L, KPNA2, RAN and RANBP1 (By similarity). Identified in a complex with nucleotide-free RAN and RCC1 (PubMed:7882974, PubMed:7616957).By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RANP628263EBI-1032909,EBI-286642

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • Ran GTPase binding Source: ProtInc

Protein-protein interaction databases

BioGridi111838. 41 interactors.
CORUMiP43487.
DIPiDIP-35058N.
IntActiP43487. 32 interactors.
MINTiP43487.
STRINGi9606.ENSP00000327583.

Structurei

Secondary structure

1201
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi48 – 58Combined sources11
Beta strandi67 – 79Combined sources13
Beta strandi81 – 83Combined sources3
Beta strandi86 – 92Combined sources7
Turni93 – 95Combined sources3
Beta strandi98 – 103Combined sources6
Beta strandi117 – 127Combined sources11
Beta strandi134 – 141Combined sources8
Helixi145 – 165Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5DX-ray2.70B/E/H/K1-201[»]
1K5GX-ray3.10B/E/H/K1-201[»]
ProteinModelPortaliP43487.
SMRiP43487.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43487.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 164RanBD1PROSITE-ProRule annotationAdd BLAST139

Sequence similaritiesi

Belongs to the RANBP1 family.Curated

Phylogenomic databases

eggNOGiKOG0864. Eukaryota.
COG5171. LUCA.
GeneTreeiENSGT00900000141073.
HOGENOMiHOG000176323.
HOVERGENiHBG006958.
InParanoidiP43487.
KOiK15306.
PhylomeDBiP43487.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR011993. PH-like_dom_sf.
IPR000156. Ran_bind_dom.
PfamiView protein in Pfam
PF00638. Ran_BP1. 1 hit.
SMARTiView protein in SMART
SM00160. RanBD. 1 hit.
PROSITEiView protein in PROSITE
PS50196. RANBD1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P43487-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAKDTHED HDTSTENTDE SNHDPQFEPI VSLPEQEIKT LEEDEEELFK
60 70 80 90 100
MRAKLFRFAS ENDLPEWKER GTGDVKLLKH KEKGAIRLLM RRDKTLKICA
110 120 130 140 150
NHYITPMMEL KPNAGSDRAW VWNTHADFAD ECPKPELLAI RFLNAENAQK
160 170 180 190 200
FKTKFEECRK EIEEREKKAG SGKNDHAEKV AEKLEALSVK EETKEDAEEK

Q
Length:201
Mass (Da):23,310
Last modified:November 1, 1995 - v1
Checksum:i05FC9B35DADA48C9
GO
Isoform 2 (identifier: P43487-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-169: Missing.

Show »
Length:200
Mass (Da):23,239
Checksum:i11E4114E1392C43D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61E → V in BAD97226 (PubMed:10591208).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03656716E → D in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_053629145A → V. Corresponds to variant dbSNP:rs5746863Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055104169Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83617 mRNA. Translation: CAA58592.1.
D38076 mRNA. Translation: BAA07269.1.
CR456556 mRNA. Translation: CAG30442.1.
AK223506 mRNA. Translation: BAD97226.1.
AC006547 Genomic DNA. No translation available.
CCDSiCCDS13775.1. [P43487-1]
CCDS63408.1. [P43487-2]
PIRiS54290.
RefSeqiNP_001265568.1. NM_001278639.1.
NP_001265569.1. NM_001278640.1. [P43487-2]
NP_002873.1. NM_002882.3. [P43487-1]
UniGeneiHs.24763.

Genome annotation databases

EnsembliENST00000331821; ENSP00000327583; ENSG00000099901. [P43487-1]
ENST00000402752; ENSP00000384925; ENSG00000099901. [P43487-2]
GeneIDi5902.
KEGGihsa:5902.
UCSCiuc002zro.3. human. [P43487-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRANG_HUMAN
AccessioniPrimary (citable) accession number: P43487
Secondary accession number(s): Q53EY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome