Reviewed, UniProtKB/Swiss-Prot P43478 (CGKA_ALTCA)

Names and origin

Protein names

Recommended name:
    Kappa-carrageenase
    EC=3.2.1.83

Gene names

Name:

cgkA

Organism

Alteromonas carrageenovora (Pseudoalteromonas carrageenovora)

Taxonomic identifier

227 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kappa-carrageenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Ref.1

Chain

26 – 397

372

Kappa-carrageenase

PRO_0000011798

Sites

Active site

163

1

Nucleophile

Active site

165

1

Active site

168

1

Proton donor

Site

260

1

Important for substrate recognition

Amino acid modifications

Disulfide bond

98 ↔ 268

Secondary structure

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397

Helix Strand Turn

Details...

Beta strand

39 – 41

3

Helix

43 – 45

3

Turn

54 – 56

3

Beta strand

57 – 60

4

Beta strand

67 – 69

3

Helix

71 – 73

3

Beta strand

74 – 77

4

Beta strand

80 – 96

17

Helix

97 – 99

3

Beta strand

101 – 112

12

Beta strand

114 – 121

8

Beta strand

123 – 131

9

Beta strand

137 – 146

10

Beta strand

159 – 168

10

Beta strand

172 – 174

3

Beta strand

177 – 179

3

Beta strand

182 – 188

7

Beta strand

191 – 195

5

Turn

197 – 199

3

Helix

201 – 204

4

Beta strand

207 – 209

3

Beta strand

219 – 225

7

Beta strand

227 – 234

8

Beta strand

237 – 243

7

Beta strand

251 – 259

9

Beta strand

263 – 267

5

Beta strand

270 – 273

4

Beta strand

283 – 296

14

Sequences

	Sequence		Length	Mass (Da)
	P43478-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: DA6D47C4682B10EF	FASTA	397	44,224
	10 20 30 40 50 60 MKPISIVAFP IPAISMLLLS AVSQAASMQP PIAKPGETWI LQAKRSDEFN VKDATKWNFQ 70 80 90 100 110 120 TENYGVWSWK NENATVSNGK LKLTTKRESH QRTFWDGCNQ QQVANYPLYY TSGVAKSRAT 130 140 150 160 170 180 GNYGYYEARI KGASTFPGVS PAFWMYSTID RSLTKEGDVQ YSEIDVVELT QKSAVRESDH 190 200 210 220 230 240 DLHNIVVKNG KPTWMRPGSF PQTNHNGYHL PFDPRNDFHT YGVNVTKDKI TWYVDGEIVG 250 260 270 280 290 300 EKDNLYWHRQ MNLTLSQGLR APHTQWKCNQ FYPSANKSAE GFPTSMEVDY VRTWVKVGNN 310 320 330 340 350 360 NSAPGEGQSC PNTFVAVNSV QLSAAKQTLR KGQSTTLEST VLPNCATNKK VIYSSSNKNV 370 380 390 ATVNSAGVVK AKNKGTATIT VKTKNKGKID KLTIAVN

References

[1]	"The gene encoding the kappa-carrageenase of Alteromonas carrageenovora is related to beta-1,3-1,4-glucanases." Barbeyron T., Henrissat B., Kloareg B. Gene 139:105-109(1994) [PubMed: 8112578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-30 AND 33-37. Strain: ATCC 43555 / DSM 6820 / IAM 12662 / IFO 12985 / NCIMB 302.
[2]	"The kappa-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases." Michel G., Chantalat L., Duee E., Barbeyron T., Henrissat B., Kloareg B., Dideberg O. Structure 9:513-525(2001) [PubMed: 11435116] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS) OF 27-297.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X71620 Genomic DNA. Translation: CAA50624.1.

PIR

I39507.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DYP	X-ray	1.54	A	27-297	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH16. Glycoside Hydrolase Family 16.

Enzyme and pathway databases

BRENDA

3.2.1.83. 141294.

Family and domain databases

InterPro

IPR003343. Big_2.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]

Gene3D

G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.

Pfam

PF02368. Big_2. 1 hit.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]

SMART

SM00635. BID_2. 1 hit.
[Graphical view]

PROSITE

PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CGKA_ALTCA

Accession

Primary (citable) accession number: P43478

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)