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P43478

- CGKA_PSEVC

UniProt

P43478 - CGKA_PSEVC

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Protein

Kappa-carrageenase

Gene

cgkA

Organism
Pseudoalteromonas carrageenovora (Alteromonas carrageenovora)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei163 – 1631Nucleophile
Active sitei165 – 1651
Active sitei168 – 1681Proton donor
Sitei260 – 2601Important for substrate recognition

GO - Molecular functioni

  1. kappa-carrageenase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16652.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-carrageenase (EC:3.2.1.83)
Gene namesi
Name:cgkA
OrganismiPseudoalteromonas carrageenovora (Alteromonas carrageenovora)
Taxonomic identifieri227 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 397372Kappa-carrageenasePRO_0000011798Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi98 ↔ 268

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
397
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi39 – 413Combined sources
Helixi43 – 453Combined sources
Turni54 – 563Combined sources
Beta strandi57 – 604Combined sources
Beta strandi67 – 693Combined sources
Helixi71 – 733Combined sources
Beta strandi74 – 774Combined sources
Beta strandi80 – 9617Combined sources
Helixi97 – 993Combined sources
Beta strandi101 – 11212Combined sources
Beta strandi114 – 1218Combined sources
Beta strandi123 – 1319Combined sources
Beta strandi137 – 14610Combined sources
Beta strandi159 – 16810Combined sources
Beta strandi172 – 1743Combined sources
Beta strandi177 – 1793Combined sources
Beta strandi182 – 1887Combined sources
Beta strandi191 – 1955Combined sources
Turni197 – 1993Combined sources
Helixi201 – 2044Combined sources
Beta strandi207 – 2093Combined sources
Beta strandi219 – 2257Combined sources
Beta strandi227 – 2348Combined sources
Beta strandi237 – 2437Combined sources
Beta strandi251 – 2599Combined sources
Beta strandi263 – 2675Combined sources
Beta strandi270 – 2734Combined sources
Beta strandi283 – 29614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DYPX-ray1.54A27-297[»]
ProteinModelPortaliP43478.
SMRiP43478. Positions 27-297.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43478.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR003343. Big_2.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR008964. Invasin/intimin_cell_adhesion.
[Graphical view]
PfamiPF02368. Big_2. 1 hit.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SMARTiSM00635. BID_2. 1 hit.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43478-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKPISIVAFP IPAISMLLLS AVSQAASMQP PIAKPGETWI LQAKRSDEFN
60 70 80 90 100
VKDATKWNFQ TENYGVWSWK NENATVSNGK LKLTTKRESH QRTFWDGCNQ
110 120 130 140 150
QQVANYPLYY TSGVAKSRAT GNYGYYEARI KGASTFPGVS PAFWMYSTID
160 170 180 190 200
RSLTKEGDVQ YSEIDVVELT QKSAVRESDH DLHNIVVKNG KPTWMRPGSF
210 220 230 240 250
PQTNHNGYHL PFDPRNDFHT YGVNVTKDKI TWYVDGEIVG EKDNLYWHRQ
260 270 280 290 300
MNLTLSQGLR APHTQWKCNQ FYPSANKSAE GFPTSMEVDY VRTWVKVGNN
310 320 330 340 350
NSAPGEGQSC PNTFVAVNSV QLSAAKQTLR KGQSTTLEST VLPNCATNKK
360 370 380 390
VIYSSSNKNV ATVNSAGVVK AKNKGTATIT VKTKNKGKID KLTIAVN
Length:397
Mass (Da):44,224
Last modified:November 1, 1995 - v1
Checksum:iDA6D47C4682B10EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71620 Genomic DNA. Translation: CAA50624.1.
PIRiI39507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71620 Genomic DNA. Translation: CAA50624.1 .
PIRi I39507.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DYP X-ray 1.54 A 27-297 [» ]
ProteinModelPortali P43478.
SMRi P43478. Positions 27-297.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16652.

Miscellaneous databases

EvolutionaryTracei P43478.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR003343. Big_2.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR008964. Invasin/intimin_cell_adhesion.
[Graphical view ]
Pfami PF02368. Big_2. 1 hit.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view ]
SMARTi SM00635. BID_2. 1 hit.
[Graphical view ]
SUPFAMi SSF49373. SSF49373. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The gene encoding the kappa-carrageenase of Alteromonas carrageenovora is related to beta-1,3-1,4-glucanases."
    Barbeyron T., Henrissat B., Kloareg B.
    Gene 139:105-109(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-30 AND 33-37.
    Strain: ATCC 43555 / DSM 6820 / IAM 12662 / NBRC 12985 / NCIMB 302.
  2. "The kappa-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases."
    Michel G., Chantalat L., Duee E., Barbeyron T., Henrissat B., Kloareg B., Dideberg O.
    Structure 9:513-525(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS) OF 27-297.

Entry informationi

Entry nameiCGKA_PSEVC
AccessioniPrimary (citable) accession number: P43478
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3