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P43478

- CGKA_PSEVC

UniProt

P43478 - CGKA_PSEVC

Protein

Kappa-carrageenase

Gene

cgkA

Organism
Pseudoalteromonas carrageenovora (Alteromonas carrageenovora)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei163 – 1631Nucleophile
    Active sitei165 – 1651
    Active sitei168 – 1681Proton donor
    Sitei260 – 2601Important for substrate recognition

    GO - Molecular functioni

    1. kappa-carrageenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16652.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kappa-carrageenase (EC:3.2.1.83)
    Gene namesi
    Name:cgkA
    OrganismiPseudoalteromonas carrageenovora (Alteromonas carrageenovora)
    Taxonomic identifieri227 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

    Subcellular locationi

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 25251 PublicationAdd
    BLAST
    Chaini26 – 397372Kappa-carrageenasePRO_0000011798Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi98 ↔ 268

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    397
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi39 – 413
    Helixi43 – 453
    Turni54 – 563
    Beta strandi57 – 604
    Beta strandi67 – 693
    Helixi71 – 733
    Beta strandi74 – 774
    Beta strandi80 – 9617
    Helixi97 – 993
    Beta strandi101 – 11212
    Beta strandi114 – 1218
    Beta strandi123 – 1319
    Beta strandi137 – 14610
    Beta strandi159 – 16810
    Beta strandi172 – 1743
    Beta strandi177 – 1793
    Beta strandi182 – 1887
    Beta strandi191 – 1955
    Turni197 – 1993
    Helixi201 – 2044
    Beta strandi207 – 2093
    Beta strandi219 – 2257
    Beta strandi227 – 2348
    Beta strandi237 – 2437
    Beta strandi251 – 2599
    Beta strandi263 – 2675
    Beta strandi270 – 2734
    Beta strandi283 – 29614

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DYPX-ray1.54A27-297[»]
    ProteinModelPortaliP43478.
    SMRiP43478. Positions 27-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43478.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR003343. Big_2.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR008964. Invasin/intimin_cell_adhesion.
    [Graphical view]
    PfamiPF02368. Big_2. 1 hit.
    PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    SMARTiSM00635. BID_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF49373. SSF49373. 1 hit.
    SSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43478-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPISIVAFP IPAISMLLLS AVSQAASMQP PIAKPGETWI LQAKRSDEFN    50
    VKDATKWNFQ TENYGVWSWK NENATVSNGK LKLTTKRESH QRTFWDGCNQ 100
    QQVANYPLYY TSGVAKSRAT GNYGYYEARI KGASTFPGVS PAFWMYSTID 150
    RSLTKEGDVQ YSEIDVVELT QKSAVRESDH DLHNIVVKNG KPTWMRPGSF 200
    PQTNHNGYHL PFDPRNDFHT YGVNVTKDKI TWYVDGEIVG EKDNLYWHRQ 250
    MNLTLSQGLR APHTQWKCNQ FYPSANKSAE GFPTSMEVDY VRTWVKVGNN 300
    NSAPGEGQSC PNTFVAVNSV QLSAAKQTLR KGQSTTLEST VLPNCATNKK 350
    VIYSSSNKNV ATVNSAGVVK AKNKGTATIT VKTKNKGKID KLTIAVN 397
    Length:397
    Mass (Da):44,224
    Last modified:November 1, 1995 - v1
    Checksum:iDA6D47C4682B10EF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71620 Genomic DNA. Translation: CAA50624.1.
    PIRiI39507.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71620 Genomic DNA. Translation: CAA50624.1 .
    PIRi I39507.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DYP X-ray 1.54 A 27-297 [» ]
    ProteinModelPortali P43478.
    SMRi P43478. Positions 27-297.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16652.

    Miscellaneous databases

    EvolutionaryTracei P43478.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR003343. Big_2.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR008964. Invasin/intimin_cell_adhesion.
    [Graphical view ]
    Pfami PF02368. Big_2. 1 hit.
    PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    SMARTi SM00635. BID_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49373. SSF49373. 1 hit.
    SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene encoding the kappa-carrageenase of Alteromonas carrageenovora is related to beta-1,3-1,4-glucanases."
      Barbeyron T., Henrissat B., Kloareg B.
      Gene 139:105-109(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-30 AND 33-37.
      Strain: ATCC 43555 / DSM 6820 / IAM 12662 / NBRC 12985 / NCIMB 302.
    2. "The kappa-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases."
      Michel G., Chantalat L., Duee E., Barbeyron T., Henrissat B., Kloareg B., Dideberg O.
      Structure 9:513-525(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS) OF 27-297.

    Entry informationi

    Entry nameiCGKA_PSEVC
    AccessioniPrimary (citable) accession number: P43478
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3