Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kappa-carrageenase

Gene

cgkA

Organism
Pseudoalteromonas carrageenovora (Alteromonas carrageenovora)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei163Nucleophile1
Active sitei1651
Active sitei168Proton donor1
Sitei260Important for substrate recognition1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16652.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-carrageenase (EC:3.2.1.83)
Gene namesi
Name:cgkA
OrganismiPseudoalteromonas carrageenovora (Alteromonas carrageenovora)
Taxonomic identifieri227 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000001179826 – 397Kappa-carrageenaseAdd BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi98 ↔ 268

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 41Combined sources3
Helixi43 – 45Combined sources3
Turni54 – 56Combined sources3
Beta strandi57 – 60Combined sources4
Beta strandi67 – 69Combined sources3
Helixi71 – 73Combined sources3
Beta strandi74 – 77Combined sources4
Beta strandi80 – 96Combined sources17
Helixi97 – 99Combined sources3
Beta strandi101 – 112Combined sources12
Beta strandi114 – 121Combined sources8
Beta strandi123 – 131Combined sources9
Beta strandi137 – 146Combined sources10
Beta strandi159 – 168Combined sources10
Beta strandi172 – 174Combined sources3
Beta strandi177 – 179Combined sources3
Beta strandi182 – 188Combined sources7
Beta strandi191 – 195Combined sources5
Turni197 – 199Combined sources3
Helixi201 – 204Combined sources4
Beta strandi207 – 209Combined sources3
Beta strandi219 – 225Combined sources7
Beta strandi227 – 234Combined sources8
Beta strandi237 – 243Combined sources7
Beta strandi251 – 259Combined sources9
Beta strandi263 – 267Combined sources5
Beta strandi270 – 273Combined sources4
Beta strandi283 – 296Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DYPX-ray1.54A27-297[»]
ProteinModelPortaliP43478.
SMRiP43478.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43478.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 299GH16PROSITE-ProRule annotationAdd BLAST274

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR003343. Big_2.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR008964. Invasin/intimin_cell_adhesion.
[Graphical view]
PfamiPF02368. Big_2. 1 hit.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SMARTiSM00635. BID_2. 1 hit.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPISIVAFP IPAISMLLLS AVSQAASMQP PIAKPGETWI LQAKRSDEFN
60 70 80 90 100
VKDATKWNFQ TENYGVWSWK NENATVSNGK LKLTTKRESH QRTFWDGCNQ
110 120 130 140 150
QQVANYPLYY TSGVAKSRAT GNYGYYEARI KGASTFPGVS PAFWMYSTID
160 170 180 190 200
RSLTKEGDVQ YSEIDVVELT QKSAVRESDH DLHNIVVKNG KPTWMRPGSF
210 220 230 240 250
PQTNHNGYHL PFDPRNDFHT YGVNVTKDKI TWYVDGEIVG EKDNLYWHRQ
260 270 280 290 300
MNLTLSQGLR APHTQWKCNQ FYPSANKSAE GFPTSMEVDY VRTWVKVGNN
310 320 330 340 350
NSAPGEGQSC PNTFVAVNSV QLSAAKQTLR KGQSTTLEST VLPNCATNKK
360 370 380 390
VIYSSSNKNV ATVNSAGVVK AKNKGTATIT VKTKNKGKID KLTIAVN
Length:397
Mass (Da):44,224
Last modified:November 1, 1995 - v1
Checksum:iDA6D47C4682B10EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71620 Genomic DNA. Translation: CAA50624.1.
PIRiI39507.

Genome annotation databases

KEGGiag:CAA50624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71620 Genomic DNA. Translation: CAA50624.1.
PIRiI39507.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DYPX-ray1.54A27-297[»]
ProteinModelPortaliP43478.
SMRiP43478.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA50624.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16652.

Miscellaneous databases

EvolutionaryTraceiP43478.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR003343. Big_2.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR008964. Invasin/intimin_cell_adhesion.
[Graphical view]
PfamiPF02368. Big_2. 1 hit.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SMARTiSM00635. BID_2. 1 hit.
[Graphical view]
SUPFAMiSSF49373. SSF49373. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCGKA_PSEVC
AccessioniPrimary (citable) accession number: P43478
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.