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P43477

- DPEP1_SHEEP

UniProt

P43477 - DPEP1_SHEEP

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Protein
Dipeptidase 1
Gene
DPEP1
Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity. In lung tissue, it may terminate or significantly reduce the leukotriene induced signal for bronchospasm.1 Publication

Catalytic activityi

Hydrolysis of dipeptides.

Cofactori

Zinc.

Enzyme regulationi

Inhibited by L-penicillamine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Zinc 1; catalytic By similarity
Metal bindingi38 – 381Zinc 1; catalytic By similarity
Metal bindingi141 – 1411Zinc 1; catalytic By similarity
Metal bindingi141 – 1411Zinc 2; catalytic By similarity
Binding sitei168 – 1681Substrate By similarity
Metal bindingi214 – 2141Zinc 2; catalytic By similarity
Metal bindingi235 – 2351Zinc 2; catalytic By similarity
Binding sitei246 – 2461Substrate By similarity
Binding sitei304 – 3041Substrate By similarity

GO - Molecular functioni

  1. GPI anchor binding Source: UniProtKB
  2. cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
  3. dipeptidyl-peptidase activity Source: InterPro
  4. metallodipeptidase activity Source: UniProtKB
  5. modified amino acid binding Source: UniProtKB
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. antibiotic metabolic process Source: UniProtKB
  2. cellular response to calcium ion Source: UniProtKB
  3. cellular response to drug Source: UniProtKB
  4. cellular response to nitric oxide Source: UniProtKB
  5. homocysteine metabolic process Source: UniProtKB
  6. negative regulation of apoptotic process Source: UniProtKB
  7. negative regulation of cell migration Source: UniProtKB
  8. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM19.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Dipeptidase 1 (EC:3.4.13.19)
Alternative name(s):
Microsomal dipeptidase
Gene namesi
Name:DPEP1
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
ProteomesiUP000002356: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. apical part of cell Source: UniProtKB
  3. apical plasma membrane Source: UniProtKB-SubCell
  4. extracellular space Source: UniProtKB
  5. microvillus membrane Source: UniProtKB-SubCell
  6. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 By similarity
Add
BLAST
Chaini17 – 384368Dipeptidase 1
PRO_0000018662Add
BLAST
Propeptidei385 – 41026Removed in mature form By similarity
PRO_0000018663Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...) By similarity
Glycosylationi62 – 621N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi87 ↔ 170 By similarity
Disulfide bondi242 ↔ 274 By similarity
Glycosylationi279 – 2791N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi377 – 377Interchain By similarity
Lipidationi384 – 3841GPI-anchor amidated serine By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Tissue specificityi

Expressed in lung, kidney and intestinal tissues.1 Publication

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Structurei

3D structure databases

ProteinModelPortaliP43477.
SMRiP43477. Positions 17-384.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M19 family.

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG002339.

Family and domain databases

InterProiIPR000180. Dipep_AS.
IPR028536. Dpep1.
IPR008257. Renal_dipep_fam.
[Graphical view]
PANTHERiPTHR10443. PTHR10443. 1 hit.
PTHR10443:SF17. PTHR10443:SF17. 1 hit.
PfamiPF01244. Peptidase_M19. 1 hit.
[Graphical view]
PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43477-1 [UniParc]FASTAAdd to Basket

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MWTGWWLWPL VAVCTADQFR DNAVRLMQST PVIDGHNDLP WALLKKFNNQ    50
LQDPRANLTS LNSTHTNIPK LKAGFVGAQF WSAYTPCDTQ NKDSVKRTVE 100
QIDVIQRMCQ LYPETFLCVT DSAGIQQAFQ EGKVASLVGV EGGHSIDSSL 150
GVLRALYHLG MRYLTLTHSC NTPWADNWLV DTGEDKAQSQ GLSSFGQSVV 200
KEMNRLGIII DLAHVSVATM EAALQLSKAP VIFSHSSAYS LCHHRRNVPD 250
HVLQLVKQTG SLVMVNFYND YVSCKAEANL SQVADHLDYI KKVAGAGAVG 300
FGGDYDGVSR LPSGLEDVSK YPDLVAELLR RQWTEEEVRG ALAENLLRVF 350
KAVEQASDHK QAPGEEPIPL GQLEASCRTK YGYSGTPSLH LQPGSLLASL 400
VTLLLSLCLL 410
Length:410
Mass (Da):45,096
Last modified:November 1, 1995 - v1
Checksum:iAA818C8B8BB91F31
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421A → Q AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27113 mRNA. Translation: AAA21725.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27113 mRNA. Translation: AAA21725.1 .

3D structure databases

ProteinModelPortali P43477.
SMRi P43477. Positions 17-384.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M19.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG002339.

Family and domain databases

InterProi IPR000180. Dipep_AS.
IPR028536. Dpep1.
IPR008257. Renal_dipep_fam.
[Graphical view ]
PANTHERi PTHR10443. PTHR10443. 1 hit.
PTHR10443:SF17. PTHR10443:SF17. 1 hit.
Pfami PF01244. Peptidase_M19. 1 hit.
[Graphical view ]
PROSITEi PS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of sheep lung dipeptidase: a glycosyl phosphatidylinositol-anchored ectoenzyme that converts leukotriene D4 to leukotriene E4."
    An S., Schmidt F.J., Campbell B.J.
    Biochim. Biophys. Acta 1226:337-340(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-56, FUNCTION, TISSUE SPECIFICITY.
    Tissue: Lung.

Entry informationi

Entry nameiDPEP1_SHEEP
AccessioniPrimary (citable) accession number: P43477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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