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Protein

Dipeptidase 1

Gene

DPEP1

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity. In lung tissue, it may terminate or significantly reduce the leukotriene induced signal for bronchospasm.1 Publication

Catalytic activityi

Hydrolysis of dipeptides.PROSITE-ProRule annotation

Cofactori

Enzyme regulationi

Inhibited by L-penicillamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Zinc 1; catalyticPROSITE-ProRule annotation1
Metal bindingi38Zinc 1; catalyticPROSITE-ProRule annotation1
Metal bindingi141Zinc 1; catalyticPROSITE-ProRule annotation1
Metal bindingi141Zinc 2; catalyticPROSITE-ProRule annotation1
Binding sitei168SubstratePROSITE-ProRule annotation1
Metal bindingi214Zinc 2; catalyticPROSITE-ProRule annotation1
Metal bindingi235Zinc 2; catalyticPROSITE-ProRule annotation1
Binding sitei246SubstratePROSITE-ProRule annotation1
Binding sitei304SubstratePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM19.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Dipeptidase 1 (EC:3.4.13.19)
Alternative name(s):
Microsomal dipeptidase
Gene namesi
Name:DPEP1
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16By similarityAdd BLAST16
ChainiPRO_000001866217 – 384Dipeptidase 1Add BLAST368
PropeptideiPRO_0000018663385 – 410Removed in mature formBy similarityAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...)By similarity1
Glycosylationi62N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi87 ↔ 170PROSITE-ProRule annotation
Disulfide bondi242 ↔ 274PROSITE-ProRule annotation
Glycosylationi279N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi377InterchainPROSITE-ProRule annotation
Lipidationi384GPI-anchor amidated serineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Tissue specificityi

Expressed in lung, kidney and intestinal tissues.1 Publication

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Structurei

3D structure databases

ProteinModelPortaliP43477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M19 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG002339.

Family and domain databases

CDDicd01301. rDP_like. 1 hit.
InterProiIPR000180. Dipep_AS.
IPR028536. Dpep1.
IPR032466. Metal_Hydrolase.
IPR008257. Renal_dipep_fam.
[Graphical view]
PANTHERiPTHR10443. PTHR10443. 1 hit.
PTHR10443:SF17. PTHR10443:SF17. 1 hit.
PfamiPF01244. Peptidase_M19. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTGWWLWPL VAVCTADQFR DNAVRLMQST PVIDGHNDLP WALLKKFNNQ
60 70 80 90 100
LQDPRANLTS LNSTHTNIPK LKAGFVGAQF WSAYTPCDTQ NKDSVKRTVE
110 120 130 140 150
QIDVIQRMCQ LYPETFLCVT DSAGIQQAFQ EGKVASLVGV EGGHSIDSSL
160 170 180 190 200
GVLRALYHLG MRYLTLTHSC NTPWADNWLV DTGEDKAQSQ GLSSFGQSVV
210 220 230 240 250
KEMNRLGIII DLAHVSVATM EAALQLSKAP VIFSHSSAYS LCHHRRNVPD
260 270 280 290 300
HVLQLVKQTG SLVMVNFYND YVSCKAEANL SQVADHLDYI KKVAGAGAVG
310 320 330 340 350
FGGDYDGVSR LPSGLEDVSK YPDLVAELLR RQWTEEEVRG ALAENLLRVF
360 370 380 390 400
KAVEQASDHK QAPGEEPIPL GQLEASCRTK YGYSGTPSLH LQPGSLLASL
410
VTLLLSLCLL
Length:410
Mass (Da):45,096
Last modified:November 1, 1995 - v1
Checksum:iAA818C8B8BB91F31
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42A → Q AA sequence (PubMed:8054366).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27113 mRNA. Translation: AAA21725.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27113 mRNA. Translation: AAA21725.1.

3D structure databases

ProteinModelPortaliP43477.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM19.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG002339.

Family and domain databases

CDDicd01301. rDP_like. 1 hit.
InterProiIPR000180. Dipep_AS.
IPR028536. Dpep1.
IPR032466. Metal_Hydrolase.
IPR008257. Renal_dipep_fam.
[Graphical view]
PANTHERiPTHR10443. PTHR10443. 1 hit.
PTHR10443:SF17. PTHR10443:SF17. 1 hit.
PfamiPF01244. Peptidase_M19. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPEP1_SHEEP
AccessioniPrimary (citable) accession number: P43477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.