Reviewed, UniProtKB/Swiss-Prot P43471 (SCRB_PEDPE)

Names and origin

Protein names

Recommended name:
    Sucrose-6-phosphate hydrolase
      Short name=Sucrase
    EC=3.2.1.26
Alternative name(s):
    Invertase

Gene names

Name:

scrB

Organism

Pediococcus pentosaceus

Taxonomic identifier

1255 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Pediococcus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Sequence caution	The sequence AAA25568.1 differs from that shown. Reason: Frameshift at positions 225 and 240. The sequence CAA83669.1 differs from that shown. Reason: Frameshift at positions 225 and 240.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Sucrose-6-phosphate hydrolase		PRO_0000169874
Regions
	Region	44 – 47	4	Substrate binding By similarity
	Region	106 – 107	2	Substrate binding By similarity
	Region	167 – 168	2	Substrate binding By similarity
Sites
	Active site	47	1	By similarity
	Binding site	63	1	Substrate By similarity
	Binding site	222	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43471-1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 7D1337951DFAFA21	FASTA	501	56,690
	10 20 30 40 50 60 MIWNRKTRYT PYEQWPATKL PQLVAQARQS KWRMQHHIQP TSGLLNDPNG FSYFDGQWHL 70 80 90 100 110 120 FYQVFPFGPV HGLKSWQHVT SKNLVDWHDE GLAIRPDTPY DSHGAYTGTA LPIDDQLFIM 130 140 150 160 170 180 YTGNVRTADW QRESYQLGAW MDTDNHIKKL SRPLIAHAPA GYTSSFRDPD LIRNDHGYYA 190 200 210 220 230 240 LIGAQTTTEI GAILVYFSKD LTTWTCQGEL NVPANARGYM IECPNLVWID QQPVLLFCPQ 250 260 270 280 290 300 GLSQATIPYQ NIYPNMYLVA DQLNLAQAQF TEPHALTQLD DGFDVYATQA INAPDGRALA 310 320 330 340 350 360 VSWIGLPEIS YPTDRENWAH CLSLVKELTL KDGHLYQNPV AAVDDLRTTA HDLVFEQQRA 370 380 390 400 410 420 TVAALNGSFE LLLTVPADKT VTVNIADQQE SGQLQVTVDA NHGQVMIDRR HTGNSFAEDY 430 440 450 460 470 480 GQTRQVELTA HKTIKIRLII DVSVFECYID NGYSVMTGRF FLNATPSRLN VQGDTTAVTG 490 500 KVWEWRQSEH TGVDNNETKI K

References

[1]	"The sucrose and raffinose operons of Pediococcus pentosaceus PPE1.0." Leenhouts K.K.J., Bolhuis A.A., Kok J.J., Venema G.G. Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PPE1.0.
[2]	"Molecular structure of the Lactobacillus plantarum sucrose utilization locus: comparison with Pediococcus pentosaceus." Naumoff D.G., Livshits V.A. Mol. Biol. (Mosk.) 35:19-27(2001) [PubMed: 11234378] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFTS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z32771 Genomic DNA. Translation: CAA83669.1. Frameshift. L32093 Genomic DNA. Translation: AAA25568.1. Frameshift.
PIR	S44258.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Enzyme and pathway databases
BRENDA	3.2.1.26. 1184.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR006232. Suc6P_hydrolase. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
TIGRFAMs	TIGR01322. scrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_PEDPE

Accession

Primary (citable) accession number: P43471

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)