Reviewed, UniProtKB/Swiss-Prot P43450 (CDK2_CARAU)

Names and origin

Protein names

Recommended name:
    Cell division protein kinase 2
    EC=2.7.11.22

Gene names

Name:

cdk2

Organism

Carassius auratus (Goldfish)

Taxonomic identifier

7957 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Carassius

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2 By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-160 activates it By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Cell division protein kinase 2		PRO_0000085773
Regions
	Domain	4 – 286	283	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
	Nucleotide binding	81 – 83	3	ATP By similarity
	Nucleotide binding	129 – 132	4	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
	Binding site	86	1	ATP By similarity
	Binding site	145	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	160	1	Phosphothreonine; by CAK By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43450-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 50A93A63A235F9A9	FASTA	298	33,999
	10 20 30 40 50 60 MESFQKVEKI GEGTYGVVYK AKNKVTGETV ALKKIRLDTE TEGVPSTAIR EISLLKELNH 70 80 90 100 110 120 PNIVKLHDVI HTENKLYLVF EFLHQDLKRF MDSSTVTGIS LPLVKSYLFQ LLQGLAFCHS 130 140 150 160 170 180 HRVLHRDLKP QNLLINAQGE IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY 190 200 210 220 230 240 STAVDIWSLG CIFAEMITRK ALFPGDSEID QLFRIFRTLG TPDESIWPGV TSMPDYKPSF 250 260 270 280 290 PKWARQDLSK VVPPLDEDGR DLLGQMLIYD PNKRISAKNA LVHRFFRDVT MPVPPLRL

References

[1]

"Isolation and characterization of goldfish cdk2, a cognate variant of the cell cycle regulator cdc2." Hirai T., Yamashita M., Yoshikuni M., Tokumoto T., Kajiura H., Sakai N., Nagahama Y. Dev. Biol. 152:113-120(1992) [PubMed: 1339336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Oocyte.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S40289 mRNA. Translation: AAB22550.1.
PIR	A44878.
3D structure databases
SMR	P43450. Positions 1-298.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P43450.
Enzyme and pathway databases
BRENDA	2.7.11.22. 141457.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK2_CARAU

Accession

Primary (citable) accession number: P43450

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)