P43439 (NTPI_ENTHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type sodium ATPase subunit I

Alternative name(s):
Na(+)-translocating ATPase subunit I
V-type sodium pump subunit I

Gene names

Name:	ntpI
Synonyms:	ntpM
Ordered Locus Names:	EHR_08235

Organism

Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258) [Complete proteome] [HAMAP]

Taxonomic identifier

768486 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Enterococcus › Enterococcus hirae

Protein attributes

Sequence length	664 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in ATP-driven sodium extrusion.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the V-ATPase 116 kDa subunit family.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell proton-transporting V-type ATPase, V0 domain Inferred from electronic annotation. Source: InterPro
Molecular_function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 664	664	V-type sodium ATPase subunit I		PRO_0000119244
Regions
	Transmembrane	373 – 393	21	Helical; Potential
	Transmembrane	460 – 480	21	Helical; Potential
	Transmembrane	496 – 516	21	Helical; Potential
	Transmembrane	522 – 542	21	Helical; Potential
	Transmembrane	574 – 594	21	Helical; Potential
	Transmembrane	601 – 621	21	Helical; Potential
Experimental info
	Sequence conflict	501	1	G → W in BAA04270. Ref.1
	Sequence conflict	515	1	I → T in BAA04270. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P43439 [UniParc]. Last modified January 9, 2013. Version 2. Checksum: A914002F489C70B6	FASTA	664	75,504
	10 20 30 40 50 60 MAVTKMEKVT LISDKKNREI LLQAVQGLHA VEIRDLFQES ENNQWVETFF PEPEMIDKDK 70 80 90 100 110 120 ELAKLSYKLT DIRTAIQFIE HHGEKSQKKQ HLKRRELSLD TLEKNYSEEA FSKKLEEVLL 130 140 150 160 170 180 LKEQWEQLVD ERQQLEDQEN WLLNWQNLDL APKAFDSQMT KLVIGTVNAK NAESFKAEVA 190 200 210 220 230 240 EINEAYLEEI NSSPTTTYFA YIVLRADESR MEEIASRYGF VKEDYLYEGT PQQQLVAAKQ 250 260 270 280 290 300 SLQEIKDQQK KLSSAIGACS GYIKDFEWTE EIFLARSERE AIKDRIIHTP YLILIQGWVD 310 320 330 340 350 360 HEEKQELIHM LQNILASEEV YLTFDEPTDN EIAEEVPTKL KNHPIVAPFE MLTEMYSLPK 370 380 390 400 410 420 YEEVDPTPWM MPFYLVFFGM MVADIGYGLL MFLGAFLLQK LVVLPRGMQR FAKFFEILAI 430 440 450 460 470 480 PSIIWGFIYS SFFGAALPKE IFGIHLPFPI LSTTDDVNTI LILSVIFGLI QILVGLFIAA 490 500 510 520 530 540 KEHIKRKAYV DAVNDGFAWQ GILLGIILIL LGTMILKNNA FVYLGGALAV LSAVCILIIP 550 560 570 580 590 600 VFQSSSKAKG IAKGAYNLYG LTGYIGDLVS YTRLMALGIS GGSIAAAFNM LVAFMPPAAR 610 620 630 640 650 660 FSVGILLIIV LQALNMFLTL LSAYVHGARL QYVEFFGKFY TGGGRSFKPL KTVEKYVNIN HKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and characterization of the ntp gene cluster for vacuolar-type Na(+)-translocating ATPase of Enterococcus hirae." Takase K., Kakinuma S., Yamato I., Konishi K., Igarashi K., Kakinuma Y. J. Biol. Chem. 269:11037-11044(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[2]	"Operon of vacuolar-type Na(+)-ATPase of Enterococcus hirae." Solioz M., Davies K. J. Biol. Chem. 269:9453-9459(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.
[3]	"Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790, a model organism for the study of ion transport, bioenergetics, and copper homeostasis." Gaechter T., Wunderlin C., Schmidheini T., Solioz M. J. Bacteriol. 194:5126-5127(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17462 Genomic DNA. Translation: BAA04270.1. X76913 Genomic DNA. Translation: CAA54236.1. CP003504 Genomic DNA. Translation: AFM70574.1.
PIR	B53610.
RefSeq	YP_006487465.1. NC_018081.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.A.2.2.2. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	13177588.
KEGG	ehr:EHR_08235.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K02123.
Enzyme and pathway databases
BioCyc	EHIR768486:GLCW-1640-MONOMER. MetaCyc:MONOMER-14154.
Family and domain databases
InterPro	IPR002490. V-ATPase_116kDa_su. [Graphical view]
PANTHER	PTHR11629. PTHR11629. 1 hit.
Pfam	PF01496. V_ATPase_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTPI_ENTHA

Accession

Primary (citable) accession number: P43439
Secondary accession number(s): I6TB87

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 9, 2013
Last modified:	May 29, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program