Reviewed, UniProtKB/Swiss-Prot P43434 (PA21B_CAVPO)

Names and origin

Protein names

Recommended name:
    Phospholipase A2
    EC=3.1.1.4
Alternative name(s):
    Phosphatidylcholine 2-acylhydrolase
    Group IB phospholipase A2

Gene names

Name:

PLA2G1B

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the phospholipase A2 family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: InterPro phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	By similarity
	Propeptide	16 – 22	7	Activation peptide		PRO_0000022735
	Chain	23 – 146	124	Phospholipase A2		PRO_0000022736
Sites
	Active site	70	1	By similarity
	Active site	121	1	By similarity
	Metal binding	50	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	52	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	54	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	71	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	33 ↔ 99		By similarity
	Disulfide bond	49 ↔ 146		By similarity
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	66 ↔ 127		By similarity
	Disulfide bond	73 ↔ 120		By similarity
	Disulfide bond	83 ↔ 113		By similarity
	Disulfide bond	106 ↔ 118		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43434-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 760DE6CD11E20E07	FASTA	146	16,187
	10 20 30 40 50 60 MKLLVLAALL SVSAAAHIIT PRALWQFRDM IKCAIPGSRP YSEYNNYGCF CGLGGSGTPV 70 80 90 100 110 120 DELDRCCEIH DACYTQAKHL ESCKSVIDNP YTNSYSFSCS GTNIICSSKN KECEEFICNC 130 140 DRAAAICFSK APYNENNKNI NKKERC

References

[1]	"Cloning and expression of phospholipase A2 from guinea pig gastric mucosa, its induction by carbachol and secretion in vivo." Ying Z., Tojo H., Nonaka Y., Okamoto M. Eur. J. Biochem. 215:91-97(1993) [PubMed: 8344290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D00740 mRNA. Translation: BAA00640.1.
PIR	S34049.
3D structure databases
HSSP	HSSP built from PDB template 1L8S based on UniProtKB P00592.
SMR	P43434. Positions 19-141.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P43434.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
ProDom	PD000303. PhospholipaseA2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21B_CAVPO

Accession

Primary (citable) accession number: P43434

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)