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Protein

Interleukin-12 subunit beta

Gene

Il12b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.By similarity
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine

Enzyme and pathway databases

ReactomeiR-MMU-447115. Interleukin-12 family signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-12 subunit beta
Short name:
IL-12B
Alternative name(s):
Cytotoxic lymphocyte maturation factor 40 kDa subunit
Short name:
CLMF p40
IL-12 subunit p40
Gene namesi
Name:Il12b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96540. Il12b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176814.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000001093323 – 335Interleukin-12 subunit betaAdd BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi50 ↔ 90PROSITE-ProRule annotation
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi132N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi197Interchain (with C-92 in IL12A)PROSITE-ProRule annotation
Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP43432.
PRIDEiP43432.

PTM databases

iPTMnetiP43432.
PhosphoSitePlusiP43432.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004296.
CleanExiMM_IL12B.
ExpressionAtlasiP43432. baseline and differential.
GenevisibleiP43432. MM.

Interactioni

Subunit structurei

Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
Il23aQ9EQ143EBI-2481353,EBI-2481329

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-6013N.
IntActiP43432. 1 interactor.
STRINGi10090.ENSMUSP00000099860.

Structurei

3D structure databases

ProteinModelPortaliP43432.
SMRiP43432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 106Ig-like C2-typeAdd BLAST84
Domaini233 – 324Fibronectin type-IIIAdd BLAST92

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi155 – 159Poly-Ser5

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

eggNOGiENOG410IF5K. Eukaryota.
ENOG410YWXR. LUCA.
GeneTreeiENSGT00390000012630.
HOGENOMiHOG000113027.
HOVERGENiHBG052094.
InParanoidiP43432.
KOiK05425.
OMAiWSTPHSY.
OrthoDBiEOG091G0EKV.
PhylomeDBiP43432.
TreeFamiTF334829.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 3 hits.
InterProiView protein in InterPro
IPR003961. FN3_dom.
IPR036116. FN3_sf.
IPR003530. Hematopoietin_rcpt_L_F3_CS.
IPR007110. Ig-like_dom.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR015528. IL-12_beta.
IPR019482. IL-12_beta_cen-dom.
PfamiView protein in Pfam
PF10420. IL12p40_C. 1 hit.
PIRSFiPIRSF038007. IL_12_beta. 1 hit.
PRINTSiPR01928. INTRLEUKN12B.
SMARTiView protein in SMART
SM00408. IGc2. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 2 hits.
PROSITEiView protein in PROSITE
PS01354. HEMATOPO_REC_L_F3. 1 hit.
PS50835. IG_LIKE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC
60 70 80 90 100
DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS
110 120 130 140 150
HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK
160 170 180 190 200
FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA
210 220 230 240 250
EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE
260 270 280 290 300
VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS
310 320 330
TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS
Length:335
Mass (Da):38,235
Last modified:November 1, 1995 - v1
Checksum:i19E4FA5EF0CFC82A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti169M → T in strain: B10.S/J and SJL/J. 1 Publication1
Natural varianti294F → L in strain: B10.S/J and SJL/J. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86671 mRNA. Translation: AAA39296.1.
S82426
, S82421, S82422, S82424, S82425 Genomic DNA. Translation: AAB37383.1.
AF128214 mRNA. Translation: AAF22555.1.
AF128215 mRNA. Translation: AAF22556.1.
CCDSiCCDS24563.1.
PIRiI72789.
RefSeqiNP_001290173.1. NM_001303244.1.
UniGeneiMm.239707.

Genome annotation databases

EnsembliENSMUST00000102796; ENSMUSP00000099860; ENSMUSG00000004296.
ENSMUST00000170513; ENSMUSP00000125867; ENSMUSG00000004296.
GeneIDi16160.
KEGGimmu:16160.
UCSCiuc007inc.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiIL12B_MOUSE
AccessioniPrimary (citable) accession number: P43432
Secondary accession number(s): Q9QUM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 22, 2017
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families