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P43432

- IL12B_MOUSE

UniProt

P43432 - IL12B_MOUSE

Protein

Interleukin-12 subunit beta

Gene

Il12b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.By similarity
    Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.1 Publication

    GO - Molecular functioni

    1. cytokine receptor activity Source: InterPro
    2. interleukin-12 alpha subunit binding Source: MGI
    3. protein binding Source: IntAct
    4. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. cell cycle arrest Source: Ensembl
    2. cell migration Source: Ensembl
    3. cell surface receptor signaling pathway Source: MGI
    4. cellular response to interferon-gamma Source: MGI
    5. cellular response to lipopolysaccharide Source: MGI
    6. defense response to Gram-negative bacterium Source: Ensembl
    7. defense response to protozoan Source: MGI
    8. defense response to virus Source: MGI
    9. natural killer cell activation Source: UniProtKB
    10. natural killer cell activation involved in immune response Source: Ensembl
    11. negative regulation of growth of symbiont in host Source: MGI
    12. negative regulation of inflammatory response to antigenic stimulus Source: MGI
    13. negative regulation of interleukin-10 production Source: Ensembl
    14. negative regulation of interleukin-17 production Source: Ensembl
    15. negative regulation of smooth muscle cell proliferation Source: Ensembl
    16. positive regulation of activated T cell proliferation Source: UniProtKB
    17. positive regulation of activation of JAK2 kinase activity Source: Ensembl
    18. positive regulation of cell adhesion Source: Ensembl
    19. positive regulation of defense response to virus by host Source: BHF-UCL
    20. positive regulation of granulocyte macrophage colony-stimulating factor production Source: Ensembl
    21. positive regulation of interferon-gamma production Source: MGI
    22. positive regulation of interleukin-10 production Source: Ensembl
    23. positive regulation of interleukin-12 production Source: Ensembl
    24. positive regulation of interleukin-17 production Source: Ensembl
    25. positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: Ensembl
    26. positive regulation of natural killer cell proliferation Source: Ensembl
    27. positive regulation of NK T cell activation Source: Ensembl
    28. positive regulation of NK T cell proliferation Source: Ensembl
    29. positive regulation of osteoclast differentiation Source: Ensembl
    30. positive regulation of smooth muscle cell apoptotic process Source: Ensembl
    31. positive regulation of T cell mediated cytotoxicity Source: BHF-UCL
    32. positive regulation of T cell proliferation Source: MGI
    33. positive regulation of T-helper 1 type immune response Source: BHF-UCL
    34. positive regulation of tumor necrosis factor production Source: Ensembl
    35. positive regulation of tyrosine phosphorylation of Stat3 protein Source: Ensembl
    36. positive regulation of tyrosine phosphorylation of Stat4 protein Source: Ensembl
    37. positive regulation of tyrosine phosphorylation of Stat5 protein Source: Ensembl
    38. regulation of tyrosine phosphorylation of Stat1 protein Source: Ensembl
    39. response to UV-B Source: Ensembl
    40. sensory perception of pain Source: Ensembl
    41. T-helper cell differentiation Source: UniProtKB

    Keywords - Molecular functioni

    Cytokine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin-12 subunit beta
    Short name:
    IL-12B
    Alternative name(s):
    Cytotoxic lymphocyte maturation factor 40 kDa subunit
    Short name:
    CLMF p40
    IL-12 subunit p40
    Gene namesi
    Name:Il12b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:96540. Il12b.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. extracellular space Source: MGI
    3. interleukin-12 complex Source: MGI
    4. interleukin-23 complex Source: Ensembl
    5. membrane Source: InterPro

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222By similarityAdd
    BLAST
    Chaini23 – 335313Interleukin-12 subunit betaPRO_0000010933Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi50 ↔ 90PROSITE-ProRule annotation
    Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi132 – 1321N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi197 – 197Interchain (with C-92 in IL12A)PROSITE-ProRule annotation
    Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP43432.

    PTM databases

    PhosphoSiteiP43432.

    Expressioni

    Gene expression databases

    BgeeiP43432.
    CleanExiMM_IL12B.
    GenevestigatoriP43432.

    Interactioni

    Subunit structurei

    Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Il23aQ9EQ143EBI-2481353,EBI-2481329

    Protein-protein interaction databases

    DIPiDIP-6013N.
    IntActiP43432. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP43432.
    SMRiP43432. Positions 24-331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 10684Ig-like C2-typeAdd
    BLAST
    Domaini233 – 32492Fibronectin type-IIIAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi155 – 1595Poly-Ser

    Sequence similaritiesi

    Contains 1 fibronectin type-III domain.Curated

    Keywords - Domaini

    Immunoglobulin domain, Signal

    Phylogenomic databases

    eggNOGiNOG45642.
    HOGENOMiHOG000113027.
    HOVERGENiHBG052094.
    InParanoidiP43432.
    KOiK05425.
    OMAiWSTPHSY.
    OrthoDBiEOG7TBC2K.
    PhylomeDBiP43432.
    TreeFamiTF334829.

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    InterProiIPR003961. Fibronectin_type3.
    IPR003530. Hematopoietin_rcpt_L_F3_CS.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003598. Ig_sub2.
    IPR015528. IL-12_beta.
    IPR019482. IL-12_beta_cen-dom.
    [Graphical view]
    PANTHERiPTHR11321. PTHR11321. 1 hit.
    PfamiPF10420. IL12p40_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038007. IL_12_beta. 1 hit.
    PRINTSiPR01928. INTRLEUKN12B.
    SMARTiSM00408. IGc2. 1 hit.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 2 hits.
    PROSITEiPS01354. HEMATOPO_REC_L_F3. 1 hit.
    PS50835. IG_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43432-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC    50
    DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS 100
    HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK 150
    FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA 200
    EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE 250
    VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS 300
    TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS 335
    Length:335
    Mass (Da):38,235
    Last modified:November 1, 1995 - v1
    Checksum:i19E4FA5EF0CFC82A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti169 – 1691M → T in strain: B10.S/J and SJL/J. 1 Publication
    Natural varianti294 – 2941F → L in strain: B10.S/J and SJL/J. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86671 mRNA. Translation: AAA39296.1.
    S82426
    , S82421, S82422, S82424, S82425 Genomic DNA. Translation: AAB37383.1.
    AF128214 mRNA. Translation: AAF22555.1.
    AF128215 mRNA. Translation: AAF22556.1.
    CCDSiCCDS24563.1.
    PIRiI72789.
    RefSeqiNP_032378.1. NM_008352.2.
    XP_006532369.1. XM_006532306.1.
    UniGeneiMm.239707.

    Genome annotation databases

    EnsembliENSMUST00000102796; ENSMUSP00000099860; ENSMUSG00000004296.
    ENSMUST00000170513; ENSMUSP00000125867; ENSMUSG00000004296.
    GeneIDi16160.
    KEGGimmu:16160.
    UCSCiuc007inb.1. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86671 mRNA. Translation: AAA39296.1 .
    S82426
    , S82421 , S82422 , S82424 , S82425 Genomic DNA. Translation: AAB37383.1 .
    AF128214 mRNA. Translation: AAF22555.1 .
    AF128215 mRNA. Translation: AAF22556.1 .
    CCDSi CCDS24563.1.
    PIRi I72789.
    RefSeqi NP_032378.1. NM_008352.2.
    XP_006532369.1. XM_006532306.1.
    UniGenei Mm.239707.

    3D structure databases

    ProteinModelPortali P43432.
    SMRi P43432. Positions 24-331.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6013N.
    IntActi P43432. 1 interaction.

    Chemistry

    ChEMBLi CHEMBL2176814.

    PTM databases

    PhosphoSitei P43432.

    Proteomic databases

    PRIDEi P43432.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102796 ; ENSMUSP00000099860 ; ENSMUSG00000004296 .
    ENSMUST00000170513 ; ENSMUSP00000125867 ; ENSMUSG00000004296 .
    GeneIDi 16160.
    KEGGi mmu:16160.
    UCSCi uc007inb.1. mouse.

    Organism-specific databases

    CTDi 3593.
    MGIi MGI:96540. Il12b.

    Phylogenomic databases

    eggNOGi NOG45642.
    HOGENOMi HOG000113027.
    HOVERGENi HBG052094.
    InParanoidi P43432.
    KOi K05425.
    OMAi WSTPHSY.
    OrthoDBi EOG7TBC2K.
    PhylomeDBi P43432.
    TreeFami TF334829.

    Miscellaneous databases

    NextBioi 288979.
    PROi P43432.
    SOURCEi Search...

    Gene expression databases

    Bgeei P43432.
    CleanExi MM_IL12B.
    Genevestigatori P43432.

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    InterProi IPR003961. Fibronectin_type3.
    IPR003530. Hematopoietin_rcpt_L_F3_CS.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003598. Ig_sub2.
    IPR015528. IL-12_beta.
    IPR019482. IL-12_beta_cen-dom.
    [Graphical view ]
    PANTHERi PTHR11321. PTHR11321. 1 hit.
    Pfami PF10420. IL12p40_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038007. IL_12_beta. 1 hit.
    PRINTSi PR01928. INTRLEUKN12B.
    SMARTi SM00408. IGc2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 2 hits.
    PROSITEi PS01354. HEMATOPO_REC_L_F3. 1 hit.
    PS50835. IG_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of murine IL-12."
      Schoenhaut D.S., Chua A.O., Wolitzky A.G., Quinn P.M., Dwyer C.M., Gately M.K., Gubler U.
      J. Immunol. 148:3433-3440(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Spleen.
    2. "Structure and chromosomal location of the mouse interleukin-12 p35 and p40 subunit genes."
      Tone Y., Thompson S.A., Babik J.M., Nolan K.F., Tone M., Raven C., Waldmann H.
      Eur. J. Immunol. 26:1222-1227(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for eae7, a locus controlling susceptibility to monophasic remitting/nonrelapsing experimental allergic encephalomyelitis."
      Teuscher C., Butterfield R.J., Ma R.Z., Zachary J.F., Doerge R.W., Blankenhorn E.P.
      J. Immunol. 163:2262-2266(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS THR-169 AND LEU-294.
      Strain: B10.S/J and SJL/J.
      Tissue: Spleen.
    4. Cited for: FUNCTION, INTERACTION WITH IL23A.

    Entry informationi

    Entry nameiIL12B_MOUSE
    AccessioniPrimary (citable) accession number: P43432
    Secondary accession number(s): Q9QUM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3