Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Interleukin-12 subunit beta

Gene

Il12b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.By similarity
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.1 Publication

GO - Molecular functioni

  1. cytokine receptor activity Source: InterPro
  2. identical protein binding Source: MGI
  3. interleukin-12 alpha subunit binding Source: MGI
  4. protein heterodimerization activity Source: MGI
  5. protein homodimerization activity Source: MGI

GO - Biological processi

  1. cell cycle arrest Source: MGI
  2. cell migration Source: MGI
  3. cell surface receptor signaling pathway Source: MGI
  4. cellular response to interferon-gamma Source: MGI
  5. cellular response to lipopolysaccharide Source: MGI
  6. defense response to Gram-negative bacterium Source: MGI
  7. defense response to protozoan Source: MGI
  8. defense response to virus Source: MGI
  9. natural killer cell activation Source: UniProtKB
  10. natural killer cell activation involved in immune response Source: Ensembl
  11. negative regulation of growth of symbiont in host Source: MGI
  12. negative regulation of inflammatory response to antigenic stimulus Source: MGI
  13. negative regulation of interleukin-10 production Source: MGI
  14. negative regulation of interleukin-17 production Source: MGI
  15. negative regulation of smooth muscle cell proliferation Source: MGI
  16. positive regulation of activated T cell proliferation Source: UniProtKB
  17. positive regulation of activation of JAK2 kinase activity Source: MGI
  18. positive regulation of cell adhesion Source: MGI
  19. positive regulation of defense response to virus by host Source: BHF-UCL
  20. positive regulation of granulocyte macrophage colony-stimulating factor production Source: MGI
  21. positive regulation of interferon-gamma production Source: MGI
  22. positive regulation of interleukin-10 production Source: MGI
  23. positive regulation of interleukin-12 production Source: MGI
  24. positive regulation of interleukin-17 production Source: MGI
  25. positive regulation of lymphocyte proliferation Source: MGI
  26. positive regulation of mononuclear cell proliferation Source: MGI
  27. positive regulation of natural killer cell activation Source: MGI
  28. positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: MGI
  29. positive regulation of natural killer cell proliferation Source: MGI
  30. positive regulation of NK T cell activation Source: MGI
  31. positive regulation of NK T cell proliferation Source: MGI
  32. positive regulation of osteoclast differentiation Source: MGI
  33. positive regulation of smooth muscle cell apoptotic process Source: MGI
  34. positive regulation of T cell mediated cytotoxicity Source: BHF-UCL
  35. positive regulation of T cell proliferation Source: MGI
  36. positive regulation of T-helper 1 type immune response Source: BHF-UCL
  37. positive regulation of tumor necrosis factor production Source: MGI
  38. positive regulation of tyrosine phosphorylation of Stat3 protein Source: MGI
  39. positive regulation of tyrosine phosphorylation of Stat4 protein Source: MGI
  40. positive regulation of tyrosine phosphorylation of Stat5 protein Source: MGI
  41. regulation of tyrosine phosphorylation of Stat1 protein Source: MGI
  42. response to UV-B Source: MGI
  43. sensory perception of pain Source: Ensembl
  44. T-helper cell differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-12 subunit beta
Short name:
IL-12B
Alternative name(s):
Cytotoxic lymphocyte maturation factor 40 kDa subunit
Short name:
CLMF p40
IL-12 subunit p40
Gene namesi
Name:Il12b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:96540. Il12b.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular space Source: MGI
  3. interleukin-12 complex Source: MGI
  4. interleukin-23 complex Source: BHF-UCL
  5. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 335313Interleukin-12 subunit betaPRO_0000010933Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi50 ↔ 90PROSITE-ProRule annotation
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi132 – 1321N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi197 – 197Interchain (with C-92 in IL12A)PROSITE-ProRule annotation
Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP43432.

PTM databases

PhosphoSiteiP43432.

Expressioni

Gene expression databases

BgeeiP43432.
CleanExiMM_IL12B.
ExpressionAtlasiP43432. baseline and differential.
GenevestigatoriP43432.

Interactioni

Subunit structurei

Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
Il23aQ9EQ143EBI-2481353,EBI-2481329

Protein-protein interaction databases

DIPiDIP-6013N.
IntActiP43432. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP43432.
SMRiP43432. Positions 24-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 10684Ig-like C2-typeAdd
BLAST
Domaini233 – 32492Fibronectin type-IIIAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi155 – 1595Poly-Ser

Sequence similaritiesi

Contains 1 fibronectin type-III domain.Curated

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

eggNOGiNOG45642.
HOGENOMiHOG000113027.
HOVERGENiHBG052094.
InParanoidiP43432.
KOiK05425.
OMAiWSTPHSY.
OrthoDBiEOG7TBC2K.
PhylomeDBiP43432.
TreeFamiTF334829.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR003961. FN3_dom.
IPR003530. Hematopoietin_rcpt_L_F3_CS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR015528. IL-12_beta.
IPR019482. IL-12_beta_cen-dom.
[Graphical view]
PANTHERiPTHR11321. PTHR11321. 1 hit.
PfamiPF10420. IL12p40_C. 1 hit.
[Graphical view]
PIRSFiPIRSF038007. IL_12_beta. 1 hit.
PRINTSiPR01928. INTRLEUKN12B.
SMARTiSM00408. IGc2. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS01354. HEMATOPO_REC_L_F3. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43432-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC
60 70 80 90 100
DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS
110 120 130 140 150
HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK
160 170 180 190 200
FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA
210 220 230 240 250
EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE
260 270 280 290 300
VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS
310 320 330
TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS
Length:335
Mass (Da):38,235
Last modified:November 1, 1995 - v1
Checksum:i19E4FA5EF0CFC82A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti169 – 1691M → T in strain: B10.S/J and SJL/J. 1 Publication
Natural varianti294 – 2941F → L in strain: B10.S/J and SJL/J. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86671 mRNA. Translation: AAA39296.1.
S82426
, S82421, S82422, S82424, S82425 Genomic DNA. Translation: AAB37383.1.
AF128214 mRNA. Translation: AAF22555.1.
AF128215 mRNA. Translation: AAF22556.1.
CCDSiCCDS24563.1.
PIRiI72789.
RefSeqiNP_032378.1. NM_008352.2.
XP_006532369.1. XM_006532306.1.
UniGeneiMm.239707.

Genome annotation databases

EnsembliENSMUST00000102796; ENSMUSP00000099860; ENSMUSG00000004296.
ENSMUST00000170513; ENSMUSP00000125867; ENSMUSG00000004296.
GeneIDi16160.
KEGGimmu:16160.
UCSCiuc007inb.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86671 mRNA. Translation: AAA39296.1.
S82426
, S82421, S82422, S82424, S82425 Genomic DNA. Translation: AAB37383.1.
AF128214 mRNA. Translation: AAF22555.1.
AF128215 mRNA. Translation: AAF22556.1.
CCDSiCCDS24563.1.
PIRiI72789.
RefSeqiNP_032378.1. NM_008352.2.
XP_006532369.1. XM_006532306.1.
UniGeneiMm.239707.

3D structure databases

ProteinModelPortaliP43432.
SMRiP43432. Positions 24-331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6013N.
IntActiP43432. 1 interaction.

Chemistry

ChEMBLiCHEMBL2176814.

PTM databases

PhosphoSiteiP43432.

Proteomic databases

PRIDEiP43432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102796; ENSMUSP00000099860; ENSMUSG00000004296.
ENSMUST00000170513; ENSMUSP00000125867; ENSMUSG00000004296.
GeneIDi16160.
KEGGimmu:16160.
UCSCiuc007inb.1. mouse.

Organism-specific databases

CTDi3593.
MGIiMGI:96540. Il12b.

Phylogenomic databases

eggNOGiNOG45642.
HOGENOMiHOG000113027.
HOVERGENiHBG052094.
InParanoidiP43432.
KOiK05425.
OMAiWSTPHSY.
OrthoDBiEOG7TBC2K.
PhylomeDBiP43432.
TreeFamiTF334829.

Miscellaneous databases

NextBioi288979.
PROiP43432.
SOURCEiSearch...

Gene expression databases

BgeeiP43432.
CleanExiMM_IL12B.
ExpressionAtlasiP43432. baseline and differential.
GenevestigatoriP43432.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR003961. FN3_dom.
IPR003530. Hematopoietin_rcpt_L_F3_CS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR015528. IL-12_beta.
IPR019482. IL-12_beta_cen-dom.
[Graphical view]
PANTHERiPTHR11321. PTHR11321. 1 hit.
PfamiPF10420. IL12p40_C. 1 hit.
[Graphical view]
PIRSFiPIRSF038007. IL_12_beta. 1 hit.
PRINTSiPR01928. INTRLEUKN12B.
SMARTiSM00408. IGc2. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS01354. HEMATOPO_REC_L_F3. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of murine IL-12."
    Schoenhaut D.S., Chua A.O., Wolitzky A.G., Quinn P.M., Dwyer C.M., Gately M.K., Gubler U.
    J. Immunol. 148:3433-3440(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Spleen.
  2. "Structure and chromosomal location of the mouse interleukin-12 p35 and p40 subunit genes."
    Tone Y., Thompson S.A., Babik J.M., Nolan K.F., Tone M., Raven C., Waldmann H.
    Eur. J. Immunol. 26:1222-1227(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for eae7, a locus controlling susceptibility to monophasic remitting/nonrelapsing experimental allergic encephalomyelitis."
    Teuscher C., Butterfield R.J., Ma R.Z., Zachary J.F., Doerge R.W., Blankenhorn E.P.
    J. Immunol. 163:2262-2266(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS THR-169 AND LEU-294.
    Strain: B10.S/J and SJL/J.
    Tissue: Spleen.
  4. Cited for: FUNCTION, INTERACTION WITH IL23A.

Entry informationi

Entry nameiIL12B_MOUSE
AccessioniPrimary (citable) accession number: P43432
Secondary accession number(s): Q9QUM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 4, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.