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P43432 (IL12B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-12 subunit beta

Short name=IL-12B
Alternative name(s):
Cytotoxic lymphocyte maturation factor 40 kDa subunit
Short name=CLMF p40
IL-12 subunit p40
Gene names
Name:Il12b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC By similarity. Ref.4

Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Ref.4

Subunit structure

Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer.

Subcellular location

Secreted.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 3 subfamily.

Contains 1 fibronectin type-III domain.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainImmunoglobulin domain
Signal
   Molecular functionCytokine
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT-helper cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

cell cycle arrest

Inferred from electronic annotation. Source: Ensembl

cell migration

Inferred from electronic annotation. Source: Ensembl

cell surface receptor signaling pathway

Inferred from direct assay PubMed 12871593. Source: MGI

cellular response to interferon-gamma

Inferred from direct assay PubMed 18025219. Source: MGI

cellular response to lipopolysaccharide

Inferred from direct assay PubMed 18025219. Source: MGI

defense response to Gram-negative bacterium

Inferred from electronic annotation. Source: Ensembl

defense response to protozoan

Inferred from mutant phenotype PubMed 9348310. Source: MGI

defense response to virus

Inferred from direct assay PubMed 17277142. Source: MGI

natural killer cell activation

Inferred from sequence or structural similarity. Source: UniProtKB

natural killer cell activation involved in immune response

Inferred from electronic annotation. Source: Ensembl

negative regulation of growth of symbiont in host

Inferred from mutant phenotype PubMed 9348310. Source: MGI

negative regulation of inflammatory response to antigenic stimulus

Inferred from mutant phenotype PubMed 16949315. Source: MGI

negative regulation of interleukin-10 production

Inferred from electronic annotation. Source: Ensembl

negative regulation of interleukin-17 production

Inferred from electronic annotation. Source: Ensembl

negative regulation of smooth muscle cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of NK T cell activation

Inferred from electronic annotation. Source: Ensembl

positive regulation of NK T cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of T cell mediated cytotoxicity

Inferred from direct assay PubMed 14688363. Source: BHF-UCL

positive regulation of T cell proliferation

Inferred from direct assay PubMed 11057672. Source: MGI

positive regulation of T-helper 1 type immune response

Inferred from direct assay PubMed 14688363. Source: BHF-UCL

positive regulation of activated T cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of activation of JAK2 kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell adhesion

Inferred from electronic annotation. Source: Ensembl

positive regulation of defense response to virus by host

Inferred from direct assay PubMed 14688363. Source: BHF-UCL

positive regulation of granulocyte macrophage colony-stimulating factor production

Inferred from electronic annotation. Source: Ensembl

positive regulation of interferon-gamma production

Inferred from mutant phenotype PubMed 16949315. Source: MGI

positive regulation of interleukin-10 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of interleukin-12 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of interleukin-17 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target

Inferred from electronic annotation. Source: Ensembl

positive regulation of natural killer cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of osteoclast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of tumor necrosis factor production

Inferred from electronic annotation. Source: Ensembl

positive regulation of tyrosine phosphorylation of Stat3 protein

Inferred from electronic annotation. Source: Ensembl

positive regulation of tyrosine phosphorylation of Stat4 protein

Inferred from electronic annotation. Source: Ensembl

positive regulation of tyrosine phosphorylation of Stat5 protein

Inferred from electronic annotation. Source: Ensembl

regulation of tyrosine phosphorylation of Stat1 protein

Inferred from electronic annotation. Source: Ensembl

response to UV-B

Inferred from electronic annotation. Source: Ensembl

sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 11737071. Source: MGI

extracellular space

Inferred from direct assay PubMed 12855817PubMed 12871593PubMed 15220916PubMed 15240714PubMed 15692051PubMed 16236719PubMed 17277142PubMed 23892723PubMed 7690439PubMed 9348310. Source: MGI

interleukin-12 complex

Inferred from direct assay PubMed 15220916. Source: MGI

interleukin-23 complex

Inferred from electronic annotation. Source: Ensembl

membrane

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncytokine receptor activity

Inferred from electronic annotation. Source: InterPro

interleukin-12 alpha subunit binding

Inferred from physical interaction PubMed 15220916. Source: MGI

protein binding

Inferred from physical interaction Ref.4. Source: IntAct

protein homodimerization activity

Inferred from direct assay PubMed 12871593. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Il23aQ9EQ143EBI-2481353,EBI-2481329

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 By similarity
Chain23 – 335313Interleukin-12 subunit beta
PRO_0000010933

Regions

Domain23 – 10684Ig-like C2-type
Domain233 – 32492Fibronectin type-III
Compositional bias155 – 1595Poly-Ser

Amino acid modifications

Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation1221N-linked (GlcNAc...) Potential
Glycosylation1321N-linked (GlcNAc...) Potential
Glycosylation2201N-linked (GlcNAc...) Potential
Disulfide bond50 ↔ 90 Potential
Disulfide bond197Interchain (with C-92 in IL12A) By similarity

Natural variations

Natural variant1691M → T in strain: B10.S/J and SJL/J. Ref.3
Natural variant2941F → L in strain: B10.S/J and SJL/J. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P43432 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 19E4FA5EF0CFC82A

FASTA33538,235
        10         20         30         40         50         60 
MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC DTPEEDDITW 

        70         80         90        100        110        120 
TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS HLLLHKKENG IWSTEILKNF 

       130        140        150        160        170        180 
KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD 

       190        200        210        220        230        240 
QRDYEKYSVS CQEDVTCPTA EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ 

       250        260        270        280        290        300 
MKPLKNSQVE VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS 

       310        320        330 
TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS 

« Hide

References

[1]"Cloning and expression of murine IL-12."
Schoenhaut D.S., Chua A.O., Wolitzky A.G., Quinn P.M., Dwyer C.M., Gately M.K., Gubler U.
J. Immunol. 148:3433-3440(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Spleen.
[2]"Structure and chromosomal location of the mouse interleukin-12 p35 and p40 subunit genes."
Tone Y., Thompson S.A., Babik J.M., Nolan K.F., Tone M., Raven C., Waldmann H.
Eur. J. Immunol. 26:1222-1227(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for eae7, a locus controlling susceptibility to monophasic remitting/nonrelapsing experimental allergic encephalomyelitis."
Teuscher C., Butterfield R.J., Ma R.Z., Zachary J.F., Doerge R.W., Blankenhorn E.P.
J. Immunol. 163:2262-2266(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS THR-169 AND LEU-294.
Strain: B10.S/J and SJL/J.
Tissue: Spleen.
[4]"Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12."
Oppmann B., Lesley R., Blom B., Timans J.C., Xu Y., Hunte B., Vega F., Yu N., Wang J., Singh K.P., Zonin F., Vaisberg E., Churakova T., Liu M.-R., Gorman D., Wagner J., Zurawski S., Liu Y.-J. expand/collapse author list , Abrams J.S., Moore K.W., Rennick D.M., de Waal-Malefyt R., Hannum C., Bazan J.F., Kastelein R.A.
Immunity 13:715-725(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH IL23A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86671 mRNA. Translation: AAA39296.1.
S82426 expand/collapse EMBL AC list , S82421, S82422, S82424, S82425 Genomic DNA. Translation: AAB37383.1.
AF128214 mRNA. Translation: AAF22555.1.
AF128215 mRNA. Translation: AAF22556.1.
CCDSCCDS24563.1.
PIRI72789.
RefSeqNP_032378.1. NM_008352.2.
XP_006532369.1. XM_006532306.1.
UniGeneMm.239707.

3D structure databases

ProteinModelPortalP43432.
SMRP43432. Positions 24-331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-6013N.
IntActP43432. 1 interaction.

Chemistry

ChEMBLCHEMBL2176814.

PTM databases

PhosphoSiteP43432.

Proteomic databases

PRIDEP43432.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102796; ENSMUSP00000099860; ENSMUSG00000004296.
ENSMUST00000170513; ENSMUSP00000125867; ENSMUSG00000004296.
GeneID16160.
KEGGmmu:16160.
UCSCuc007inb.1. mouse.

Organism-specific databases

CTD3593.
MGIMGI:96540. Il12b.

Phylogenomic databases

eggNOGNOG45642.
HOGENOMHOG000113027.
HOVERGENHBG052094.
InParanoidP43432.
KOK05425.
OMAWSTPHSY.
OrthoDBEOG7TBC2K.
PhylomeDBP43432.
TreeFamTF334829.

Gene expression databases

BgeeP43432.
CleanExMM_IL12B.
GenevestigatorP43432.

Family and domain databases

Gene3D2.60.40.10. 3 hits.
InterProIPR003961. Fibronectin_type3.
IPR003530. Hematopoietin_rcpt_L_F3_CS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR015528. IL-12_beta.
IPR019482. IL-12_beta_cen-dom.
[Graphical view]
PANTHERPTHR11321. PTHR11321. 1 hit.
PfamPF10420. IL12p40_C. 1 hit.
[Graphical view]
PIRSFPIRSF038007. IL_12_beta. 1 hit.
PRINTSPR01928. INTRLEUKN12B.
SMARTSM00408. IGc2. 1 hit.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
PROSITEPS01354. HEMATOPO_REC_L_F3. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288979.
PROP43432.
SOURCESearch...

Entry information

Entry nameIL12B_MOUSE
AccessionPrimary (citable) accession number: P43432
Secondary accession number(s): Q9QUM1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot