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Protein

Interleukin-12 subunit alpha

Gene

Il12a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Enzyme and pathway databases

ReactomeiR-MMU-447115. Interleukin-12 signaling.
R-MMU-6788467. IL-6-type cytokine receptor ligand interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-12 subunit alpha
Short name:
IL-12A
Alternative name(s):
Cytotoxic lymphocyte maturation factor 35 kDa subunit
Short name:
CLMF p35
IL-12 subunit p35
Gene namesi
Name:Il12a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:96539. Il12a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular space Source: MGI
  • interleukin-12 complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 215193Interleukin-12 subunit alphaPRO_0000015608Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi60 ↔ 192By similarity
Disulfide bondi81 ↔ 119By similarity
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence analysis
Disulfide bondi92 – 92Interchain (with C-197 in IL12B)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP43431.
PRIDEiP43431.

Expressioni

Gene expression databases

BgeeiP43431.
CleanExiMM_IL12A.
ExpressionAtlasiP43431. baseline and differential.
GenevisibleiP43431. MM.

Interactioni

Subunit structurei

Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60363N.
IntActiP43431. 1 interaction.
STRINGi10090.ENSMUSP00000029345.

Structurei

3D structure databases

ProteinModelPortaliP43431.
SMRiP43431. Positions 33-215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-6 superfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVIR. Eukaryota.
ENOG410ZGHJ. LUCA.
HOGENOMiHOG000113026.
HOVERGENiHBG063691.
InParanoidiP43431.
KOiK05406.
PhylomeDBiP43431.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR004281. IL-12_alpha.
[Graphical view]
PANTHERiPTHR10523. PTHR10523. 1 hit.
PfamiPF03039. IL12. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCQSRYLLFL ATLALLNHLS LARVIPVSGP ARCLSQSRNL LKTTDDMVKT
60 70 80 90 100
AREKLKHYSC TAEDIDHEDI TRDQTSTLKT CLPLELHKNE SCLATRETSS
110 120 130 140 150
TTRGSCLPPQ KTSLMMTLCL GSIYEDLKMY QTEFQAINAA LQNHNHQQII
160 170 180 190 200
LDKGMLVAID ELMQSLNHNG ETLRQKPPVG EADPYRVKMK LCILLHAFST
210
RVVTINRVMG YLSSA
Length:215
Mass (Da):24,179
Last modified:November 1, 1995 - v1
Checksum:i56845B9B66D35A53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86672 mRNA. Translation: AAA39292.1.
S82419
, S82412, S82414, S82417, S82418 Genomic DNA. Translation: AAB37382.1.
D63334 Genomic DNA. Translation: BAA09647.1.
CCDSiCCDS17400.1.
PIRiI56135.
RefSeqiNP_001152896.1. NM_001159424.2.
NP_032377.1. NM_008351.3.
UniGeneiMm.103783.

Genome annotation databases

EnsembliENSMUST00000107816; ENSMUSP00000103446; ENSMUSG00000027776.
GeneIDi16159.
KEGGimmu:16159.
UCSCiuc008plv.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86672 mRNA. Translation: AAA39292.1.
S82419
, S82412, S82414, S82417, S82418 Genomic DNA. Translation: AAB37382.1.
D63334 Genomic DNA. Translation: BAA09647.1.
CCDSiCCDS17400.1.
PIRiI56135.
RefSeqiNP_001152896.1. NM_001159424.2.
NP_032377.1. NM_008351.3.
UniGeneiMm.103783.

3D structure databases

ProteinModelPortaliP43431.
SMRiP43431. Positions 33-215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60363N.
IntActiP43431. 1 interaction.
STRINGi10090.ENSMUSP00000029345.

Proteomic databases

PaxDbiP43431.
PRIDEiP43431.

Protocols and materials databases

DNASUi16159.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107816; ENSMUSP00000103446; ENSMUSG00000027776.
GeneIDi16159.
KEGGimmu:16159.
UCSCiuc008plv.3. mouse.

Organism-specific databases

CTDi3592.
MGIiMGI:96539. Il12a.

Phylogenomic databases

eggNOGiENOG410IVIR. Eukaryota.
ENOG410ZGHJ. LUCA.
HOGENOMiHOG000113026.
HOVERGENiHBG063691.
InParanoidiP43431.
KOiK05406.
PhylomeDBiP43431.

Enzyme and pathway databases

ReactomeiR-MMU-447115. Interleukin-12 signaling.
R-MMU-6788467. IL-6-type cytokine receptor ligand interactions.

Miscellaneous databases

PROiP43431.
SOURCEiSearch...

Gene expression databases

BgeeiP43431.
CleanExiMM_IL12A.
ExpressionAtlasiP43431. baseline and differential.
GenevisibleiP43431. MM.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR004281. IL-12_alpha.
[Graphical view]
PANTHERiPTHR10523. PTHR10523. 1 hit.
PfamiPF03039. IL12. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of murine IL-12."
    Schoenhaut D.S., Chua A.O., Wolitzky A.G., Quinn P.M., Dwyer C.M., Gately M.K., Gubler U.
    J. Immunol. 148:3433-3440(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Spleen.
  2. "Structure and chromosomal location of the mouse interleukin-12 p35 and p40 subunit genes."
    Tone Y., Thompson S.A., Babik J.M., Nolan K.F., Tone M., Raven C., Waldmann H.
    Eur. J. Immunol. 26:1222-1227(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Molecular cloning and characterization of murine IL-12 genes."
    Yoshimoto T., Kojima K., Funakoshi T., Endo Y., Fujita T., Nariuchi H.
    J. Immunol. 156:1082-1088(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
    Strain: 129/Sv.

Entry informationi

Entry nameiIL12A_MOUSE
AccessioniPrimary (citable) accession number: P43431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 8, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.