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Protein

Mast cell protease 8

Gene

Mcpt8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Charge relay systemBy similarity
Active sitei107 – 1071Charge relay systemBy similarity
Active sitei200 – 2001Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

  1. proteolysis Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.254.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell protease 8 (EC:3.4.21.-)
Short name:
mMCP-8
Gene namesi
Name:Mcpt8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:1261780. Mcpt8.

Subcellular locationi

Secreted By similarity. Cytoplasmic granule By similarity
Note: Secretory granules.By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. secretory granule Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Propeptidei20 – 201Activation peptidePRO_0000027457
Chaini21 – 247227Mast cell protease 8PRO_0000027458Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi141 ↔ 206PROSITE-ProRule annotation
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP43430.
PRIDEiP43430.

Expressioni

Gene expression databases

BgeeiP43430.
CleanExiMM_MCPT8.
ExpressionAtlasiP43430. baseline and differential.
GenevestigatoriP43430.

Structurei

3D structure databases

ProteinModelPortaliP43430.
SMRiP43430. Positions 21-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 242222Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP43430.
KOiK01362.
OMAiEAIPHES.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP43430.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43430-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFLLLVLLVA ALPVNAEGGE IIWGTESKPH SRPYMAYIRF NDSKSVYRCG
60 70 80 90 100
GFLVARDIVM TAAHCNGKVI NVTLGIHNLK KKKNTQLIPV SEAIPHESFD
110 120 130 140 150
NETLVNDIML LKLERKAQLN SAVDTIALPK SKDWVKPGQV CTVAGWGKLA
160 170 180 190 200
NCTLSDTLQE VNLEVQKGQK CRSMSQTYND SIQLCVGNPS ENKATGKGDS
210 220 230 240
GGPFVCNGVV QGIVSCRLCT GTLPRVFTRI SSFMPWIRKT MKLLQQP
Length:247
Mass (Da):27,122
Last modified:November 1, 1995 - v1
Checksum:i4BB3C1D650185E29
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821I → T in AAD43900. (PubMed:11398967)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78545 mRNA. Translation: CAA55291.1.
AF119362 Genomic DNA. Translation: AAD43899.1.
AF119363 Genomic DNA. Translation: AAD43900.1.
CCDSiCCDS27141.1.
PIRiS45113.
RefSeqiNP_032598.1. NM_008572.1.
UniGeneiMm.41979.

Genome annotation databases

EnsembliENSMUST00000015594; ENSMUSP00000015594; ENSMUSG00000022157.
GeneIDi17231.
KEGGimmu:17231.
UCSCiuc007ubm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78545 mRNA. Translation: CAA55291.1.
AF119362 Genomic DNA. Translation: AAD43899.1.
AF119363 Genomic DNA. Translation: AAD43900.1.
CCDSiCCDS27141.1.
PIRiS45113.
RefSeqiNP_032598.1. NM_008572.1.
UniGeneiMm.41979.

3D structure databases

ProteinModelPortaliP43430.
SMRiP43430. Positions 21-242.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.254.

Proteomic databases

MaxQBiP43430.
PRIDEiP43430.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015594; ENSMUSP00000015594; ENSMUSG00000022157.
GeneIDi17231.
KEGGimmu:17231.
UCSCiuc007ubm.2. mouse.

Organism-specific databases

CTDi17231.
MGIiMGI:1261780. Mcpt8.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP43430.
KOiK01362.
OMAiEAIPHES.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP43430.
TreeFamiTF333630.

Miscellaneous databases

NextBioi291660.
PROiP43430.
SOURCEiSearch...

Gene expression databases

BgeeiP43430.
CleanExiMM_MCPT8.
ExpressionAtlasiP43430. baseline and differential.
GenevestigatoriP43430.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of mouse mast cell protease-8, the first member of a novel subfamily of mouse mast cell serine proteases, distinct from both the classical chymases and tryptases."
    Lutzelschwab C., Huang M.R., Kullberg M.C., Aveskogh M., Hellman L.
    Eur. J. Immunol. 28:1022-1033(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Leaden X A1.
  2. "Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes."
    Lunderius C., Hellman L.
    Immunogenetics 53:225-232(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ and ICR X Swiss Webster.
    Tissue: Liver.

Entry informationi

Entry nameiMCPT8_MOUSE
AccessioniPrimary (citable) accession number: P43430
Secondary accession number(s): Q9R1F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 4, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.