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P43430 (MCPT8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mast cell protease 8

Short name=mMCP-8
EC=3.4.21.-
Gene names
Name:Mcpt8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Secreted By similarity. Cytoplasmic granule By similarity. Note: Secretory granules By similarity.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide201Activation peptide
PRO_0000027457
Chain21 – 247227Mast cell protease 8
PRO_0000027458

Regions

Domain21 – 242222Peptidase S1

Sites

Active site641Charge relay system By similarity
Active site1071Charge relay system By similarity
Active site2001Charge relay system By similarity

Amino acid modifications

Glycosylation411N-linked (GlcNAc...) Potential
Glycosylation711N-linked (GlcNAc...) Potential
Glycosylation1011N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation1791N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 65 By similarity
Disulfide bond141 ↔ 206 By similarity
Disulfide bond171 ↔ 185 By similarity

Experimental info

Sequence conflict1821I → T in AAD43900. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P43430 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 4BB3C1D650185E29

FASTA24727,122
        10         20         30         40         50         60 
MFLLLVLLVA ALPVNAEGGE IIWGTESKPH SRPYMAYIRF NDSKSVYRCG GFLVARDIVM 

        70         80         90        100        110        120 
TAAHCNGKVI NVTLGIHNLK KKKNTQLIPV SEAIPHESFD NETLVNDIML LKLERKAQLN 

       130        140        150        160        170        180 
SAVDTIALPK SKDWVKPGQV CTVAGWGKLA NCTLSDTLQE VNLEVQKGQK CRSMSQTYND 

       190        200        210        220        230        240 
SIQLCVGNPS ENKATGKGDS GGPFVCNGVV QGIVSCRLCT GTLPRVFTRI SSFMPWIRKT 


MKLLQQP 

« Hide

References

[1]"Characterization of mouse mast cell protease-8, the first member of a novel subfamily of mouse mast cell serine proteases, distinct from both the classical chymases and tryptases."
Lutzelschwab C., Huang M.R., Kullberg M.C., Aveskogh M., Hellman L.
Eur. J. Immunol. 28:1022-1033(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Leaden X A1.
[2]"Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes."
Lunderius C., Hellman L.
Immunogenetics 53:225-232(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ and ICR X Swiss Webster.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78545 mRNA. Translation: CAA55291.1.
AF119362 Genomic DNA. Translation: AAD43899.1.
AF119363 Genomic DNA. Translation: AAD43900.1.
PIRS45113.
RefSeqNP_032598.1. NM_008572.1.
UniGeneMm.41979.

3D structure databases

ProteinModelPortalP43430.
SMRP43430. Positions 21-242.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.254.

Proteomic databases

PRIDEP43430.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000015594; ENSMUSP00000015594; ENSMUSG00000022157.
GeneID17231.
KEGGmmu:17231.
UCSCuc007ubm.2. mouse.

Organism-specific databases

CTD17231.
MGIMGI:1261780. Mcpt8.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP43430.
KOK01362.
OMAEAIPHES.
OrthoDBEOG7RRF7Z.
PhylomeDBP43430.
TreeFamTF333630.

Gene expression databases

ArrayExpressP43430.
BgeeP43430.
CleanExMM_MCPT8.
GenevestigatorP43430.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291660.
PROP43430.
SOURCESearch...

Entry information

Entry nameMCPT8_MOUSE
AccessionPrimary (citable) accession number: P43430
Secondary accession number(s): Q9R1F1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot