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P43430

- MCPT8_MOUSE

UniProt

P43430 - MCPT8_MOUSE

Protein

Mast cell protease 8

Gene

Mcpt8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei64 – 641Charge relay systemBy similarity
    Active sitei107 – 1071Charge relay systemBy similarity
    Active sitei200 – 2001Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: RefGenome

    GO - Biological processi

    1. granzyme-mediated apoptotic signaling pathway Source: RefGenome
    2. proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.254.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mast cell protease 8 (EC:3.4.21.-)
    Short name:
    mMCP-8
    Gene namesi
    Name:Mcpt8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1261780. Mcpt8.

    Subcellular locationi

    Secreted By similarity. Cytoplasmic granule By similarity
    Note: Secretory granules.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. secretory granule Source: RefGenome

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 201Activation peptidePRO_0000027457
    Chaini21 – 247227Mast cell protease 8PRO_0000027458Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi141 ↔ 206PROSITE-ProRule annotation
    Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
    Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP43430.
    PRIDEiP43430.

    Expressioni

    Gene expression databases

    ArrayExpressiP43430.
    BgeeiP43430.
    CleanExiMM_MCPT8.
    GenevestigatoriP43430.

    Structurei

    3D structure databases

    ProteinModelPortaliP43430.
    SMRiP43430. Positions 21-242.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 242222Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP43430.
    KOiK01362.
    OMAiICAKENK.
    OrthoDBiEOG7RRF7Z.
    PhylomeDBiP43430.
    TreeFamiTF333630.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43430-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLLLVLLVA ALPVNAEGGE IIWGTESKPH SRPYMAYIRF NDSKSVYRCG    50
    GFLVARDIVM TAAHCNGKVI NVTLGIHNLK KKKNTQLIPV SEAIPHESFD 100
    NETLVNDIML LKLERKAQLN SAVDTIALPK SKDWVKPGQV CTVAGWGKLA 150
    NCTLSDTLQE VNLEVQKGQK CRSMSQTYND SIQLCVGNPS ENKATGKGDS 200
    GGPFVCNGVV QGIVSCRLCT GTLPRVFTRI SSFMPWIRKT MKLLQQP 247
    Length:247
    Mass (Da):27,122
    Last modified:November 1, 1995 - v1
    Checksum:i4BB3C1D650185E29
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti182 – 1821I → T in AAD43900. (PubMed:11398967)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78545 mRNA. Translation: CAA55291.1.
    AF119362 Genomic DNA. Translation: AAD43899.1.
    AF119363 Genomic DNA. Translation: AAD43900.1.
    CCDSiCCDS27141.1.
    PIRiS45113.
    RefSeqiNP_032598.1. NM_008572.1.
    UniGeneiMm.41979.

    Genome annotation databases

    EnsembliENSMUST00000015594; ENSMUSP00000015594; ENSMUSG00000022157.
    GeneIDi17231.
    KEGGimmu:17231.
    UCSCiuc007ubm.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78545 mRNA. Translation: CAA55291.1 .
    AF119362 Genomic DNA. Translation: AAD43899.1 .
    AF119363 Genomic DNA. Translation: AAD43900.1 .
    CCDSi CCDS27141.1.
    PIRi S45113.
    RefSeqi NP_032598.1. NM_008572.1.
    UniGenei Mm.41979.

    3D structure databases

    ProteinModelPortali P43430.
    SMRi P43430. Positions 21-242.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.254.

    Proteomic databases

    MaxQBi P43430.
    PRIDEi P43430.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000015594 ; ENSMUSP00000015594 ; ENSMUSG00000022157 .
    GeneIDi 17231.
    KEGGi mmu:17231.
    UCSCi uc007ubm.2. mouse.

    Organism-specific databases

    CTDi 17231.
    MGIi MGI:1261780. Mcpt8.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P43430.
    KOi K01362.
    OMAi ICAKENK.
    OrthoDBi EOG7RRF7Z.
    PhylomeDBi P43430.
    TreeFami TF333630.

    Miscellaneous databases

    NextBioi 291660.
    PROi P43430.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P43430.
    Bgeei P43430.
    CleanExi MM_MCPT8.
    Genevestigatori P43430.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of mouse mast cell protease-8, the first member of a novel subfamily of mouse mast cell serine proteases, distinct from both the classical chymases and tryptases."
      Lutzelschwab C., Huang M.R., Kullberg M.C., Aveskogh M., Hellman L.
      Eur. J. Immunol. 28:1022-1033(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Leaden X A1.
    2. "Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes."
      Lunderius C., Hellman L.
      Immunogenetics 53:225-232(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ and ICR X Swiss Webster.
      Tissue: Liver.

    Entry informationi

    Entry nameiMCPT8_MOUSE
    AccessioniPrimary (citable) accession number: P43430
    Secondary accession number(s): Q9R1F1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3