P43430 (MCPT8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mast cell protease 8
Short name=mMCP-8
EC=3.4.21.-

Gene names

Name:

Mcpt8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Secreted By similarity. Cytoplasmic granule By similarity. Note: Secretory granules By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20	1	Activation peptide		PRO_0000027457
	Chain	21 – 247	227	Mast cell protease 8		PRO_0000027458
Regions
	Domain	21 – 242	222	Peptidase S1
Sites
	Active site	64	1	Charge relay system By similarity
	Active site	107	1	Charge relay system By similarity
	Active site	200	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Disulfide bond	49 ↔ 65		By similarity
	Disulfide bond	141 ↔ 206		By similarity
	Disulfide bond	171 ↔ 185		By similarity
Experimental info
	Sequence conflict	182	1	I → T in AAD43900. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P43430 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4BB3C1D650185E29	FASTA	247	27,122
	10 20 30 40 50 60 MFLLLVLLVA ALPVNAEGGE IIWGTESKPH SRPYMAYIRF NDSKSVYRCG GFLVARDIVM 70 80 90 100 110 120 TAAHCNGKVI NVTLGIHNLK KKKNTQLIPV SEAIPHESFD NETLVNDIML LKLERKAQLN 130 140 150 160 170 180 SAVDTIALPK SKDWVKPGQV CTVAGWGKLA NCTLSDTLQE VNLEVQKGQK CRSMSQTYND 190 200 210 220 230 240 SIQLCVGNPS ENKATGKGDS GGPFVCNGVV QGIVSCRLCT GTLPRVFTRI SSFMPWIRKT MKLLQQP

References

[1]	"Characterization of mouse mast cell protease-8, the first member of a novel subfamily of mouse mast cell serine proteases, distinct from both the classical chymases and tryptases." Lutzelschwab C., Huang M.R., Kullberg M.C., Aveskogh M., Hellman L. Eur. J. Immunol. 28:1022-1033(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Leaden X A1.
[2]	"Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes." Lunderius C., Hellman L. Immunogenetics 53:225-232(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ and ICR X Swiss Webster. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78545 mRNA. Translation: CAA55291.1. AF119362 Genomic DNA. Translation: AAD43899.1. AF119363 Genomic DNA. Translation: AAD43900.1.
IPI	IPI00120351.
PIR	S45113.
RefSeq	NP_032598.1. NM_008572.1.
UniGene	Mm.41979.
3D structure databases
ProteinModelPortal	P43430.
SMR	P43430. Positions 21-242.
ModBase	Search...
Protein family/group databases
MEROPS	S01.254.
Proteomic databases
PRIDE	P43430.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000015594; ENSMUSP00000015594; ENSMUSG00000022157.
GeneID	17231.
KEGG	mmu:17231.
Organism-specific databases
CTD	17231.
MGI	MGI:1261780. Mcpt8.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P43430.
KO	K01362.
OMA	EAIPHES.
OrthoDB	EOG47WNQP.
Gene expression databases
ArrayExpress	P43430.
Bgee	P43430.
CleanEx	MM_MCPT8.
Genevestigator	P43430.
GermOnline	ENSMUSG00000022157. Mus musculus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291660.
SOURCE	Search...

Entry information

Entry name

MCPT8_MOUSE

Accession

Primary (citable) accession number: P43430
Secondary accession number(s): Q9R1F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program