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Protein

Erythroid transcription factor

Gene

Gata1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri204 – 228GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri258 – 282GATA-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • anatomical structure formation involved in morphogenesis Source: GO_Central
  • animal organ morphogenesis Source: GO_Central
  • animal organ regeneration Source: RGD
  • basophil differentiation Source: InterPro
  • cell fate commitment Source: GO_Central
  • cellular response to cAMP Source: RGD
  • cellular response to follicle-stimulating hormone stimulus Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • digestive tract development Source: GO_Central
  • eosinophil differentiation Source: InterPro
  • erythrocyte development Source: InterPro
  • heart development Source: GO_Central
  • megakaryocyte differentiation Source: InterPro
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of mast cell degranulation Source: RGD
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription, DNA-templated Source: RGD
  • Sertoli cell development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Erythroid transcription factor
Alternative name(s):
Eryf1
GATA-binding factor 1
Short name:
GATA-1
Short name:
GF-1
NF-E1 DNA-binding protein
Gene namesi
Name:Gata1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2663. Gata1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833991 – 413Erythroid transcription factorAdd BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineBy similarity1
Modified residuei49PhosphoserineBy similarity1
Modified residuei72PhosphoserineBy similarity1
Cross-linki137Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei142PhosphoserineBy similarity1
Modified residuei178PhosphoserineBy similarity1
Modified residuei187PhosphoserineBy similarity1
Modified residuei233N6-acetyllysine; by EP300By similarity1
Modified residuei245N6-acetyllysine; by EP300By similarity1
Modified residuei246N6-acetyllysine; by CREBBPBy similarity1
Modified residuei246N6-acetyllysine; by EP300By similarity1
Modified residuei252N6-acetyllysine; by CREBBPBy similarity1
Modified residuei308N6-acetyllysineBy similarity1
Modified residuei310PhosphoserineBy similarity1
Modified residuei312N6-acetyllysine; by CREBBPBy similarity1
Modified residuei314N6-acetyllysineBy similarity1
Modified residuei315N6-acetyllysineBy similarity1

Post-translational modificationi

Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity).By similarity
Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity).By similarity
Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo. Acetylated on Lys-233, Lys-245 Lys-246 by EP300 (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP43429.

PTM databases

PhosphoSitePlusiP43429.

Expressioni

Tissue specificityi

Erythrocytes, and fetal hepatocytes.1 Publication

Gene expression databases

BgeeiENSRNOG00000025534.
GenevisibleiP43429. RN.

Interactioni

Subunit structurei

Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with BRD3 (By similarity). Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with MED1, CCAR1 and CALCOCO1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067336.

Structurei

3D structure databases

ProteinModelPortaliP43429.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni200 – 330Interaction with MED1 and CCAR1By similarityAdd BLAST131
Regioni203 – 222Required for interaction with ZFPM1By similarityAdd BLAST20
Regioni249 – 315Interaction with CALCOCO1By similarityAdd BLAST67

Domaini

The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding (By similarity).By similarity

Sequence similaritiesi

Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri204 – 228GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri258 – 282GATA-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP43429.
KOiK09182.
OMAiPSTAHLY.
OrthoDBiEOG091G0AUR.
PhylomeDBiP43429.
TreeFamiTF315391.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029524. GATA-1.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF190. PTHR10071:SF190. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43429-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFPGLGALG TSEPLPQFVD SALVSSTSDS AGFFSSGPES LDTASSSTSP
60 70 80 90 100
NAATAAATAL AYYREAEAYR HSPVFQVYPL LNSMEGIPGS SPYASWAYSK
110 120 130 140 150
TALYPASTVC PSHEDAPSQT LEDPDGKNNN TFLETLKTER LSPDLLTLGT
160 170 180 190 200
ALPTSLPVTS SAYGGADFPS PFFSPTGSPL SSAAYSSPKF HGSLPLAPCE
210 220 230 240 250
ARECVNCGAT ATPLWRRDRT GHYLCNACGL YHKMNGQNRP LIRPKKRMIV
260 270 280 290 300
SKRAGTQCTN CQTTTTTLWR RNASGDPVCN ACGLYYKLHQ VNRPLTMRKD
310 320 330 340 350
GIQTRNRKAS GKGKKKRGSS LAGAGAAEGP AGGFMVVAGG SSSGNCGEVA
360 370 380 390 400
PGLTLGTAGT AHLYQGLGPV VLSGPVSHLM SFPGPLLGSP TASFPTGPVP
410
TTTSTSVVSP LSS
Length:413
Mass (Da):42,871
Last modified:November 1, 1995 - v1
Checksum:i9BE4120FCF8269BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13518 mRNA. Translation: BAA02735.1.
PIRiS48756.
RefSeqiNP_036896.1. NM_012764.1.
XP_017457744.1. XM_017602255.1.
UniGeneiRn.10024.

Genome annotation databases

EnsembliENSRNOT00000029272; ENSRNOP00000038111; ENSRNOG00000025534.
ENSRNOT00000075082; ENSRNOP00000067336; ENSRNOG00000047663.
GeneIDi108348091.
25172.
KEGGirno:103689972.
rno:25172.
UCSCiRGD:2663. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13518 mRNA. Translation: BAA02735.1.
PIRiS48756.
RefSeqiNP_036896.1. NM_012764.1.
XP_017457744.1. XM_017602255.1.
UniGeneiRn.10024.

3D structure databases

ProteinModelPortaliP43429.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067336.

PTM databases

PhosphoSitePlusiP43429.

Proteomic databases

PaxDbiP43429.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029272; ENSRNOP00000038111; ENSRNOG00000025534.
ENSRNOT00000075082; ENSRNOP00000067336; ENSRNOG00000047663.
GeneIDi108348091.
25172.
KEGGirno:103689972.
rno:25172.
UCSCiRGD:2663. rat.

Organism-specific databases

CTDi103689972.
2623.
RGDi2663. Gata1.

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP43429.
KOiK09182.
OMAiPSTAHLY.
OrthoDBiEOG091G0AUR.
PhylomeDBiP43429.
TreeFamiTF315391.

Enzyme and pathway databases

ReactomeiR-RNO-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

PROiP43429.

Gene expression databases

BgeeiENSRNOG00000025534.
GenevisibleiP43429. RN.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029524. GATA-1.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF190. PTHR10071:SF190. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA1_RAT
AccessioniPrimary (citable) accession number: P43429
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.