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P43428

- G6PC_RAT

UniProt

P43428 - G6PC_RAT

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Protein

Glucose-6-phosphatase

Gene

G6pc

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + H2O = D-glucose + phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831SubstrateSequence Analysis
Active sitei119 – 1191Proton donorSequence Analysis
Binding sitei170 – 1701SubstrateSequence Analysis
Active sitei176 – 1761NucleophileBy similarity

GO - Molecular functioni

  1. glucose-6-phosphatase activity Source: RGD

GO - Biological processi

  1. gluconeogenesis Source: RGD
  2. glucose 6-phosphate metabolic process Source: RGD
  3. glucose homeostasis Source: RGD
  4. lipid homeostasis Source: RGD
  5. phosphate-containing compound metabolic process Source: RGD
  6. phosphorylated carbohydrate dephosphorylation Source: RGD
  7. response to food Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Gluconeogenesis

Enzyme and pathway databases

SABIO-RKP43428.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphatase (EC:3.1.3.9)
Short name:
G-6-Pase
Short name:
G6Pase
Gene namesi
Name:G6pc
Synonyms:G6pt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi2644. G6pc.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Glucose-6-phosphatasePRO_0000087415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi96 – 961N-linked (GlcNAc...)By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP43428.

Expressioni

Gene expression databases

ExpressionAtlasiP43428. baseline and differential.
GenevestigatoriP43428.

Interactioni

Protein-protein interaction databases

MINTiMINT-4577794.

Structurei

3D structure databases

ProteinModelPortaliP43428.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828LumenalSequence AnalysisAdd
BLAST
Topological domaini50 – 6011CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini82 – 11736LumenalSequence AnalysisAdd
BLAST
Topological domaini139 – 1479CytoplasmicSequence Analysis
Topological domaini169 – 1702LumenalSequence Analysis
Topological domaini192 – 21120CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini233 – 25422LumenalSequence AnalysisAdd
BLAST
Topological domaini276 – 29116CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini313 – 3208LumenalSequence Analysis
Topological domaini342 – 35716CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 4921HelicalSequence AnalysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Transmembranei148 – 16821HelicalSequence AnalysisAdd
BLAST
Transmembranei171 – 19121HelicalSequence AnalysisAdd
BLAST
Transmembranei212 – 23221HelicalSequence AnalysisAdd
BLAST
Transmembranei255 – 27521HelicalSequence AnalysisAdd
BLAST
Transmembranei292 – 31221HelicalSequence AnalysisAdd
BLAST
Transmembranei321 – 34121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi354 – 3574Prevents secretion from ERSequence Analysis

Sequence similaritiesi

Belongs to the glucose-6-phosphatase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046461.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiP43428.
KOiK01084.
OMAiGSSINTH.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

P43428-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEERMNVLHD FGIQSTRYLQ VNYEDSQDWF VLVSVIADLR NAFYVLFPIW
60 70 80 90 100
FHIQETVGIN LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP
110 120 130 140 150
LIKQFPVTCE TGPGSPSGHA MGTAGVYYVM VTSTLAIFRG KKKSTYGFRC
160 170 180 190 200
LNVVLWLGYW AVQLNVCLSR IYLAAHFPHQ VVAGVLSGIA VAETFSHIRG
210 220 230 240 250
IYNASLQRYC LITFFLFGFA LGFYLLLKGL GVDLLWTLEK AKRWCERPEW
260 270 280 290 300
VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELRK SLPFRLACIV
310 320 330 340 350
ASLGLLHLFD SLKPPSQIES IFYILSFCKS ATVPFASVSL IPYCLARLLG

QTHKKSL
Length:357
Mass (Da):40,556
Last modified:November 1, 1995 - v1
Checksum:iC44960E102F4244D
GO

Sequence cautioni

The sequence AAA74381.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH90067.2 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181G → V in AAA19966. (PubMed:8198588)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L37333 mRNA. Translation: AAA74381.1. Different initiation.
U07993 mRNA. Translation: AAA19966.1.
D78592 mRNA. Translation: BAA24348.1.
BC090067 mRNA. Translation: AAH90067.2. Different initiation.
PIRiJC2371.
RefSeqiNP_037230.2. NM_013098.2.
UniGeneiRn.10992.

Genome annotation databases

EnsembliENSRNOT00000028033; ENSRNOP00000028033; ENSRNOG00000036806.
GeneIDi25634.
KEGGirno:25634.
UCSCiRGD:2644. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L37333 mRNA. Translation: AAA74381.1 . Different initiation.
U07993 mRNA. Translation: AAA19966.1 .
D78592 mRNA. Translation: BAA24348.1 .
BC090067 mRNA. Translation: AAH90067.2 . Different initiation.
PIRi JC2371.
RefSeqi NP_037230.2. NM_013098.2.
UniGenei Rn.10992.

3D structure databases

ProteinModelPortali P43428.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4577794.

Chemistry

BindingDBi P43428.
ChEMBLi CHEMBL4759.

Proteomic databases

PRIDEi P43428.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000028033 ; ENSRNOP00000028033 ; ENSRNOG00000036806 .
GeneIDi 25634.
KEGGi rno:25634.
UCSCi RGD:2644. rat.

Organism-specific databases

CTDi 2538.
RGDi 2644. G6pc.

Phylogenomic databases

GeneTreei ENSGT00510000046461.
HOGENOMi HOG000264239.
HOVERGENi HBG003560.
InParanoidi P43428.
KOi K01084.
OMAi GSSINTH.

Enzyme and pathway databases

UniPathwayi UPA00138 .
SABIO-RK P43428.

Miscellaneous databases

NextBioi 607447.

Gene expression databases

ExpressionAtlasi P43428. baseline and differential.
Genevestigatori P43428.

Family and domain databases

Gene3Di 1.20.144.10. 1 hit.
InterProi IPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view ]
Pfami PF01569. PAP2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTi SM00014. acidPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF48317. SSF48317. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "High levels of glucose-6-phosphatase gene and protein expression reflect an adaptive response in proliferating liver and diabetes."
    Haber B.A., Chin S., Chuang E., Buikuisen W., Naji A., Taub R.A.
    J. Clin. Invest. 95:832-841(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP."
    Lange A.J., Argaud D.M., El-Maghrabi M.R., Pan W., Subir M., Pilkis S.J.
    Biochem. Biophys. Res. Commun. 201:302-309(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "Expression and distribution of glucose-6-phosphatase catalytic subunit messenger RNA and its changes in the diabetic state."
    Shingu R., Nakajima H., Horikawa Y., Hamaguchi T., Yamasaki T., Miyagawa J., Namba M., Hanafusa T., Matsuzawa Y.
    Res. Commun. Mol. Pathol. Pharmacol. 93:13-24(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiG6PC_RAT
AccessioniPrimary (citable) accession number: P43428
Secondary accession number(s): Q5FVC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3