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Protein

Glucose-6-phosphatase

Gene

G6pc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + H2O = D-glucose + phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei83SubstrateSequence analysis1
Active sitei119Proton donorSequence analysis1
Binding sitei170SubstrateSequence analysis1
Active sitei176NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cholesterol homeostasis Source: Ensembl
  • gluconeogenesis Source: RGD
  • glucose 6-phosphate metabolic process Source: RGD
  • glucose-6-phosphate transport Source: Ensembl
  • glucose homeostasis Source: RGD
  • glycogen catabolic process Source: Ensembl
  • lipid homeostasis Source: RGD
  • multicellular organism growth Source: Ensembl
  • phosphate-containing compound metabolic process Source: RGD
  • phosphorylated carbohydrate dephosphorylation Source: RGD
  • regulation of gene expression Source: Ensembl
  • response to food Source: RGD
  • steroid metabolic process Source: Ensembl
  • triglyceride metabolic process Source: Ensembl
  • urate metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Gluconeogenesis

Enzyme and pathway databases

ReactomeiR-RNO-70153. Glucose transport.
SABIO-RKP43428.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphatase (EC:3.1.3.9)
Short name:
G-6-Pase
Short name:
G6Pase
Gene namesi
Name:G6pc
Synonyms:G6pt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2644. G6pc.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28LumenalSequence analysisAdd BLAST28
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Topological domaini50 – 60CytoplasmicSequence analysisAdd BLAST11
Transmembranei61 – 81HelicalSequence analysisAdd BLAST21
Topological domaini82 – 117LumenalSequence analysisAdd BLAST36
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Topological domaini139 – 147CytoplasmicSequence analysis9
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 170LumenalSequence analysis2
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21
Topological domaini192 – 211CytoplasmicSequence analysisAdd BLAST20
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Topological domaini233 – 254LumenalSequence analysisAdd BLAST22
Transmembranei255 – 275HelicalSequence analysisAdd BLAST21
Topological domaini276 – 291CytoplasmicSequence analysisAdd BLAST16
Transmembranei292 – 312HelicalSequence analysisAdd BLAST21
Topological domaini313 – 320LumenalSequence analysis8
Transmembranei321 – 341HelicalSequence analysisAdd BLAST21
Topological domaini342 – 357CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4759.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000874151 – 357Glucose-6-phosphataseAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi96N-linked (GlcNAc...)By similarity1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP43428.

Expressioni

Gene expression databases

BgeeiENSRNOG00000036806.
GenevisibleiP43428. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4577794.

Chemistry databases

BindingDBiP43428.

Structurei

3D structure databases

ProteinModelPortaliP43428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi354 – 357Prevents secretion from ERSequence analysis4

Sequence similaritiesi

Belongs to the glucose-6-phosphatase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046465.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiP43428.
KOiK01084.
OMAiYLQVNYQ.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

P43428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEERMNVLHD FGIQSTRYLQ VNYEDSQDWF VLVSVIADLR NAFYVLFPIW
60 70 80 90 100
FHIQETVGIN LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP
110 120 130 140 150
LIKQFPVTCE TGPGSPSGHA MGTAGVYYVM VTSTLAIFRG KKKSTYGFRC
160 170 180 190 200
LNVVLWLGYW AVQLNVCLSR IYLAAHFPHQ VVAGVLSGIA VAETFSHIRG
210 220 230 240 250
IYNASLQRYC LITFFLFGFA LGFYLLLKGL GVDLLWTLEK AKRWCERPEW
260 270 280 290 300
VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELRK SLPFRLACIV
310 320 330 340 350
ASLGLLHLFD SLKPPSQIES IFYILSFCKS ATVPFASVSL IPYCLARLLG

QTHKKSL
Length:357
Mass (Da):40,556
Last modified:November 1, 1995 - v1
Checksum:iC44960E102F4244D
GO

Sequence cautioni

The sequence AAA74381 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH90067 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti118G → V in AAA19966 (PubMed:8198588).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37333 mRNA. Translation: AAA74381.1. Different initiation.
U07993 mRNA. Translation: AAA19966.1.
D78592 mRNA. Translation: BAA24348.1.
BC090067 mRNA. Translation: AAH90067.2. Different initiation.
PIRiJC2371.
RefSeqiNP_037230.2. NM_013098.2.
UniGeneiRn.10992.

Genome annotation databases

EnsembliENSRNOT00000028033; ENSRNOP00000028033; ENSRNOG00000051171.
ENSRNOT00000085831; ENSRNOP00000071453; ENSRNOG00000051171.
GeneIDi25634.
KEGGirno:25634.
UCSCiRGD:2644. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37333 mRNA. Translation: AAA74381.1. Different initiation.
U07993 mRNA. Translation: AAA19966.1.
D78592 mRNA. Translation: BAA24348.1.
BC090067 mRNA. Translation: AAH90067.2. Different initiation.
PIRiJC2371.
RefSeqiNP_037230.2. NM_013098.2.
UniGeneiRn.10992.

3D structure databases

ProteinModelPortaliP43428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4577794.

Chemistry databases

BindingDBiP43428.
ChEMBLiCHEMBL4759.

Proteomic databases

PRIDEiP43428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028033; ENSRNOP00000028033; ENSRNOG00000051171.
ENSRNOT00000085831; ENSRNOP00000071453; ENSRNOG00000051171.
GeneIDi25634.
KEGGirno:25634.
UCSCiRGD:2644. rat.

Organism-specific databases

CTDi2538.
RGDi2644. G6pc.

Phylogenomic databases

GeneTreeiENSGT00510000046465.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiP43428.
KOiK01084.
OMAiYLQVNYQ.

Enzyme and pathway databases

UniPathwayiUPA00138.
ReactomeiR-RNO-70153. Glucose transport.
SABIO-RKP43428.

Miscellaneous databases

PROiP43428.

Gene expression databases

BgeeiENSRNOG00000036806.
GenevisibleiP43428. RN.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG6PC_RAT
AccessioniPrimary (citable) accession number: P43428
Secondary accession number(s): Q5FVC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.