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Protein

Glucose-6-phosphatase

Gene

G6pc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + H2O = D-glucose + phosphate.

Pathway: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831SubstrateSequence Analysis
Active sitei119 – 1191Proton donorSequence Analysis
Binding sitei170 – 1701SubstrateSequence Analysis
Active sitei176 – 1761NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

  • cholesterol homeostasis Source: Ensembl
  • gluconeogenesis Source: RGD
  • glucose 6-phosphate metabolic process Source: RGD
  • glucose-6-phosphate transport Source: Ensembl
  • glucose homeostasis Source: RGD
  • glycogen catabolic process Source: Ensembl
  • lipid homeostasis Source: RGD
  • multicellular organism growth Source: Ensembl
  • phosphate-containing compound metabolic process Source: RGD
  • phosphorylated carbohydrate dephosphorylation Source: RGD
  • regulation of gene expression Source: Ensembl
  • response to food Source: RGD
  • steroid metabolic process Source: Ensembl
  • triglyceride metabolic process Source: Ensembl
  • urate metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Gluconeogenesis

Enzyme and pathway databases

ReactomeiREACT_275920. Glucose transport.
SABIO-RKP43428.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphatase (EC:3.1.3.9)
Short name:
G-6-Pase
Short name:
G6Pase
Gene namesi
Name:G6pc
Synonyms:G6pt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2644. G6pc.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828LumenalSequence AnalysisAdd
BLAST
Transmembranei29 – 4921HelicalSequence AnalysisAdd
BLAST
Topological domaini50 – 6011CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence AnalysisAdd
BLAST
Topological domaini82 – 11736LumenalSequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Topological domaini139 – 1479CytoplasmicSequence Analysis
Transmembranei148 – 16821HelicalSequence AnalysisAdd
BLAST
Topological domaini169 – 1702LumenalSequence Analysis
Transmembranei171 – 19121HelicalSequence AnalysisAdd
BLAST
Topological domaini192 – 21120CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei212 – 23221HelicalSequence AnalysisAdd
BLAST
Topological domaini233 – 25422LumenalSequence AnalysisAdd
BLAST
Transmembranei255 – 27521HelicalSequence AnalysisAdd
BLAST
Topological domaini276 – 29116CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei292 – 31221HelicalSequence AnalysisAdd
BLAST
Topological domaini313 – 3208LumenalSequence Analysis
Transmembranei321 – 34121HelicalSequence AnalysisAdd
BLAST
Topological domaini342 – 35716CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Glucose-6-phosphatasePRO_0000087415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi96 – 961N-linked (GlcNAc...)By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP43428.

Expressioni

Gene expression databases

GenevisibleiP43428. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4577794.

Structurei

3D structure databases

ProteinModelPortaliP43428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi354 – 3574Prevents secretion from ERSequence Analysis

Sequence similaritiesi

Belongs to the glucose-6-phosphatase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046465.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiP43428.
KOiK01084.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

P43428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEERMNVLHD FGIQSTRYLQ VNYEDSQDWF VLVSVIADLR NAFYVLFPIW
60 70 80 90 100
FHIQETVGIN LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP
110 120 130 140 150
LIKQFPVTCE TGPGSPSGHA MGTAGVYYVM VTSTLAIFRG KKKSTYGFRC
160 170 180 190 200
LNVVLWLGYW AVQLNVCLSR IYLAAHFPHQ VVAGVLSGIA VAETFSHIRG
210 220 230 240 250
IYNASLQRYC LITFFLFGFA LGFYLLLKGL GVDLLWTLEK AKRWCERPEW
260 270 280 290 300
VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELRK SLPFRLACIV
310 320 330 340 350
ASLGLLHLFD SLKPPSQIES IFYILSFCKS ATVPFASVSL IPYCLARLLG

QTHKKSL
Length:357
Mass (Da):40,556
Last modified:November 1, 1995 - v1
Checksum:iC44960E102F4244D
GO

Sequence cautioni

The sequence AAA74381.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH90067.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181G → V in AAA19966 (PubMed:8198588).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37333 mRNA. Translation: AAA74381.1. Different initiation.
U07993 mRNA. Translation: AAA19966.1.
D78592 mRNA. Translation: BAA24348.1.
BC090067 mRNA. Translation: AAH90067.2. Different initiation.
PIRiJC2371.
RefSeqiNP_037230.2. NM_013098.2.
UniGeneiRn.10992.

Genome annotation databases

EnsembliENSRNOT00000028033; ENSRNOP00000028033; ENSRNOG00000051171.
GeneIDi25634.
KEGGirno:25634.
UCSCiRGD:2644. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37333 mRNA. Translation: AAA74381.1. Different initiation.
U07993 mRNA. Translation: AAA19966.1.
D78592 mRNA. Translation: BAA24348.1.
BC090067 mRNA. Translation: AAH90067.2. Different initiation.
PIRiJC2371.
RefSeqiNP_037230.2. NM_013098.2.
UniGeneiRn.10992.

3D structure databases

ProteinModelPortaliP43428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4577794.

Chemistry

BindingDBiP43428.
ChEMBLiCHEMBL4759.

Proteomic databases

PRIDEiP43428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028033; ENSRNOP00000028033; ENSRNOG00000051171.
GeneIDi25634.
KEGGirno:25634.
UCSCiRGD:2644. rat.

Organism-specific databases

CTDi2538.
RGDi2644. G6pc.

Phylogenomic databases

GeneTreeiENSGT00510000046465.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiP43428.
KOiK01084.

Enzyme and pathway databases

UniPathwayiUPA00138.
ReactomeiREACT_275920. Glucose transport.
SABIO-RKP43428.

Miscellaneous databases

NextBioi607447.
PROiP43428.

Gene expression databases

GenevisibleiP43428. RN.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "High levels of glucose-6-phosphatase gene and protein expression reflect an adaptive response in proliferating liver and diabetes."
    Haber B.A., Chin S., Chuang E., Buikuisen W., Naji A., Taub R.A.
    J. Clin. Invest. 95:832-841(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP."
    Lange A.J., Argaud D.M., El-Maghrabi M.R., Pan W., Subir M., Pilkis S.J.
    Biochem. Biophys. Res. Commun. 201:302-309(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "Expression and distribution of glucose-6-phosphatase catalytic subunit messenger RNA and its changes in the diabetic state."
    Shingu R., Nakajima H., Horikawa Y., Hamaguchi T., Yamasaki T., Miyagawa J., Namba M., Hanafusa T., Matsuzawa Y.
    Res. Commun. Mol. Pathol. Pharmacol. 93:13-24(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiG6PC_RAT
AccessioniPrimary (citable) accession number: P43428
Secondary accession number(s): Q5FVC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.