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P43428

- G6PC_RAT

UniProt

P43428 - G6PC_RAT

Protein

Glucose-6-phosphatase

Gene

G6pc

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels By similarity.By similarity

    Catalytic activityi

    D-glucose 6-phosphate + H2O = D-glucose + phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei83 – 831SubstrateSequence Analysis
    Active sitei119 – 1191Proton donorSequence Analysis
    Binding sitei170 – 1701SubstrateSequence Analysis
    Active sitei176 – 1761NucleophileBy similarity

    GO - Molecular functioni

    1. glucose-6-phosphatase activity Source: RGD

    GO - Biological processi

    1. gluconeogenesis Source: RGD
    2. glucose 6-phosphate metabolic process Source: RGD
    3. glucose homeostasis Source: RGD
    4. lipid homeostasis Source: RGD
    5. phosphate-containing compound metabolic process Source: RGD
    6. phosphorylated carbohydrate dephosphorylation Source: RGD
    7. response to food Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Gluconeogenesis

    Enzyme and pathway databases

    SABIO-RKP43428.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphatase (EC:3.1.3.9)
    Short name:
    G-6-Pase
    Short name:
    G6Pase
    Gene namesi
    Name:G6pc
    Synonyms:G6pt
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi2644. G6pc.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 357357Glucose-6-phosphatasePRO_0000087415Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi96 – 961N-linked (GlcNAc...)By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP43428.

    Expressioni

    Gene expression databases

    GenevestigatoriP43428.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4577794.

    Structurei

    3D structure databases

    ProteinModelPortaliP43428.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2828LumenalSequence AnalysisAdd
    BLAST
    Topological domaini50 – 6011CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini82 – 11736LumenalSequence AnalysisAdd
    BLAST
    Topological domaini139 – 1479CytoplasmicSequence Analysis
    Topological domaini169 – 1702LumenalSequence Analysis
    Topological domaini192 – 21120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini233 – 25422LumenalSequence AnalysisAdd
    BLAST
    Topological domaini276 – 29116CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini313 – 3208LumenalSequence Analysis
    Topological domaini342 – 35716CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei29 – 4921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei61 – 8121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei118 – 13821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei148 – 16821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei171 – 19121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei212 – 23221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei255 – 27521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei292 – 31221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei321 – 34121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi354 – 3574Prevents secretion from ERSequence Analysis

    Sequence similaritiesi

    Belongs to the glucose-6-phosphatase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00510000046461.
    HOGENOMiHOG000264239.
    HOVERGENiHBG003560.
    InParanoidiP43428.
    KOiK01084.
    OMAiGSSINTH.

    Family and domain databases

    Gene3Di1.20.144.10. 1 hit.
    InterProiIPR016275. Glucose-6-phosphatase.
    IPR000326. P_Acid_Pase_2/haloperoxidase.
    [Graphical view]
    PfamiPF01569. PAP2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
    SMARTiSM00014. acidPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48317. SSF48317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P43428-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEERMNVLHD FGIQSTRYLQ VNYEDSQDWF VLVSVIADLR NAFYVLFPIW    50
    FHIQETVGIN LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP 100
    LIKQFPVTCE TGPGSPSGHA MGTAGVYYVM VTSTLAIFRG KKKSTYGFRC 150
    LNVVLWLGYW AVQLNVCLSR IYLAAHFPHQ VVAGVLSGIA VAETFSHIRG 200
    IYNASLQRYC LITFFLFGFA LGFYLLLKGL GVDLLWTLEK AKRWCERPEW 250
    VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELRK SLPFRLACIV 300
    ASLGLLHLFD SLKPPSQIES IFYILSFCKS ATVPFASVSL IPYCLARLLG 350
    QTHKKSL 357
    Length:357
    Mass (Da):40,556
    Last modified:November 1, 1995 - v1
    Checksum:iC44960E102F4244D
    GO

    Sequence cautioni

    The sequence AAA74381.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH90067.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti118 – 1181G → V in AAA19966. (PubMed:8198588)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L37333 mRNA. Translation: AAA74381.1. Different initiation.
    U07993 mRNA. Translation: AAA19966.1.
    D78592 mRNA. Translation: BAA24348.1.
    BC090067 mRNA. Translation: AAH90067.2. Different initiation.
    PIRiJC2371.
    RefSeqiNP_037230.2. NM_013098.2.
    UniGeneiRn.10992.

    Genome annotation databases

    EnsembliENSRNOT00000028033; ENSRNOP00000028033; ENSRNOG00000036806.
    GeneIDi25634.
    KEGGirno:25634.
    UCSCiRGD:2644. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L37333 mRNA. Translation: AAA74381.1 . Different initiation.
    U07993 mRNA. Translation: AAA19966.1 .
    D78592 mRNA. Translation: BAA24348.1 .
    BC090067 mRNA. Translation: AAH90067.2 . Different initiation.
    PIRi JC2371.
    RefSeqi NP_037230.2. NM_013098.2.
    UniGenei Rn.10992.

    3D structure databases

    ProteinModelPortali P43428.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4577794.

    Chemistry

    BindingDBi P43428.
    ChEMBLi CHEMBL4759.

    Proteomic databases

    PRIDEi P43428.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000028033 ; ENSRNOP00000028033 ; ENSRNOG00000036806 .
    GeneIDi 25634.
    KEGGi rno:25634.
    UCSCi RGD:2644. rat.

    Organism-specific databases

    CTDi 2538.
    RGDi 2644. G6pc.

    Phylogenomic databases

    GeneTreei ENSGT00510000046461.
    HOGENOMi HOG000264239.
    HOVERGENi HBG003560.
    InParanoidi P43428.
    KOi K01084.
    OMAi GSSINTH.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    SABIO-RK P43428.

    Miscellaneous databases

    NextBioi 607447.

    Gene expression databases

    Genevestigatori P43428.

    Family and domain databases

    Gene3Di 1.20.144.10. 1 hit.
    InterProi IPR016275. Glucose-6-phosphatase.
    IPR000326. P_Acid_Pase_2/haloperoxidase.
    [Graphical view ]
    Pfami PF01569. PAP2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000905. Glucose-6-phosphatase. 1 hit.
    SMARTi SM00014. acidPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48317. SSF48317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "High levels of glucose-6-phosphatase gene and protein expression reflect an adaptive response in proliferating liver and diabetes."
      Haber B.A., Chin S., Chuang E., Buikuisen W., Naji A., Taub R.A.
      J. Clin. Invest. 95:832-841(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP."
      Lange A.J., Argaud D.M., El-Maghrabi M.R., Pan W., Subir M., Pilkis S.J.
      Biochem. Biophys. Res. Commun. 201:302-309(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
    3. "Expression and distribution of glucose-6-phosphatase catalytic subunit messenger RNA and its changes in the diabetic state."
      Shingu R., Nakajima H., Horikawa Y., Hamaguchi T., Yamasaki T., Miyagawa J., Namba M., Hanafusa T., Matsuzawa Y.
      Res. Commun. Mol. Pathol. Pharmacol. 93:13-24(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiG6PC_RAT
    AccessioniPrimary (citable) accession number: P43428
    Secondary accession number(s): Q5FVC9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3