Reviewed, UniProtKB/Swiss-Prot P43428 (G6PC_RAT)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphatase
      Short name=G-6-Pase
      Short name=G6Pase
    EC=3.1.3.9

Gene names

Name:	G6pc
Synonyms:	G6pt

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels By similarity.
Catalytic activity	D-glucose 6-phosphate + H2O = D-glucose + phosphate.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the glucose-6-phosphatase family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	gluconeogenesis Ref.3 Inferred from direct assay. Source: RGD glucose 6-phosphate metabolic process Inferred from direct assay. Source: RGD phosphorylated carbohydrate dephosphorylation Inferred from direct assay. Source: RGD response to food Inferred from expression pattern. Source: RGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from direct assay. Source: RGD
Molecular function	glucose-6-phosphatase activity Ref.3 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Glucose-6-phosphatase		PRO_0000087415
Regions
	Topological domain	1 – 28	28	Lumenal Potential
	Transmembrane	29 – 49	21	Potential
	Topological domain	50 – 60	11	Cytoplasmic Potential
	Transmembrane	61 – 81	21	Potential
	Topological domain	82 – 117	36	Lumenal Potential
	Transmembrane	118 – 138	21	Potential
	Topological domain	139 – 147	9	Cytoplasmic Potential
	Transmembrane	148 – 168	21	Potential
	Topological domain	169 – 170	2	Lumenal Potential
	Transmembrane	171 – 191	21	Potential
	Topological domain	192 – 211	20	Cytoplasmic Potential
	Transmembrane	212 – 232	21	Potential
	Topological domain	233 – 254	22	Lumenal Potential
	Transmembrane	255 – 275	21	Potential
	Topological domain	276 – 291	16	Cytoplasmic Potential
	Transmembrane	292 – 312	21	Potential
	Topological domain	313 – 320	8	Lumenal Potential
	Transmembrane	321 – 341	21	Potential
	Topological domain	342 – 357	16	Cytoplasmic Potential
	Motif	354 – 357	4	Prevents secretion from ER Potential
Sites
	Active site	119	1	Proton donor Potential
	Active site	176	1	Nucleophile By similarity
	Binding site	83	1	Substrate Potential
	Binding site	170	1	Substrate Potential
Amino acid modifications
	Glycosylation	96	1	N-linked (GlcNAc...) By similarity
Experimental info
	Sequence conflict	118	1	G → V in AAA19966. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P43428-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C44960E102F4244D	FASTA	357	40,556
	10 20 30 40 50 60 MEERMNVLHD FGIQSTRYLQ VNYEDSQDWF VLVSVIADLR NAFYVLFPIW FHIQETVGIN 70 80 90 100 110 120 LLWVAVVGDW FNLVFKWILF GQRPYWWVLD TDYYSNSSVP LIKQFPVTCE TGPGSPSGHA 130 140 150 160 170 180 MGTAGVYYVM VTSTLAIFRG KKKSTYGFRC LNVVLWLGYW AVQLNVCLSR IYLAAHFPHQ 190 200 210 220 230 240 VVAGVLSGIA VAETFSHIRG IYNASLQRYC LITFFLFGFA LGFYLLLKGL GVDLLWTLEK 250 260 270 280 290 300 AKRWCERPEW VHLDTTPFAS LFKNLGTLLG LGLALNSSMY RKSCKGELRK SLPFRLACIV 310 320 330 340 350 ASLGLLHLFD SLKPPSQIES IFYILSFCKS ATVPFASVSL IPYCLARLLG QTHKKSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"High levels of glucose-6-phosphatase gene and protein expression reflect an adaptive response in proliferating liver and diabetes." Haber B.A., Chin S., Chuang E., Buikuisen W., Naji A., Taub R.A. J. Clin. Invest. 95:832-841(1995) [PubMed: 7860767] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP." Lange A.J., Argaud D.M., El-Maghrabi M.R., Pan W., Subir M., Pilkis S.J. Biochem. Biophys. Res. Commun. 201:302-309(1994) [PubMed: 8198588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[3]	"Expression and distribution of glucose-6-phosphatase catalytic subunit messenger RNA and its changes in the diabetic state." Shingu R., Nakajima H., Horikawa Y., Hamaguchi T., Yamasaki T., Miyagawa J., Namba M., Hanafusa T., Matsuzawa Y. Res. Commun. Mol. Pathol. Pharmacol. 93:13-24(1996) [PubMed: 8865366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L37333 mRNA. Translation: AAA74381.1. Different initiation. U07993 mRNA. Translation: AAA19966.1. D78592 mRNA. Translation: BAA24348.1. BC090067 mRNA. Translation: AAH90067.2. Different initiation.
IPI	IPI00200360.
PIR	JC2371.
RefSeq	NP_037230.2.
UniGene	Rn.10992
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P43428.
Genome annotation databases
Ensembl	ENSRNOG00000036805. Rattus norvegicus. [Contig view]
GeneID	25634.
KEGG	rno:25634.
Organism-specific databases
RGD	2644. G6pc.
Phylogenomic databases
HOVERGEN	P43428.
Enzyme and pathway databases
BRENDA	3.1.3.9. 248.
Gene expression databases
ArrayExpress	P43428.
GermOnline	ENSRNOG00000036805. Rattus norvegicus.
Family and domain databases
InterPro	IPR016275. Glucose-6-phosphatase. IPR000326. P_Acid_Pase_2/haloperoxidase. [Graphical view]
Pfam	PF01569. PAP2. 1 hit. [Graphical view]
PIRSF	PIRSF000905. Glucose-6-phosphatase. 1 hit.
SMART	SM00014. acidPPc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	607447.

Entry information

Entry name

G6PC_RAT

Accession

Primary (citable) accession number: P43428
Secondary accession number(s): Q5FVC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)