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Protein

Solute carrier family 2, facilitated glucose transporter member 5

Gene

Slc2a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a fructose transporter that has only low activity with other monosaccharides (PubMed:8333543, PubMed:9820812, PubMed:26416735). Can mediate the uptake of deoxyglucose, but with low efficiency (PubMed:8333543). Essential for fructose uptake in the small intestine. Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose. Required for the development of high blood pressure in response to high dietary fructose intake.By similarity3 Publications

Enzyme regulationi

Fructose uptake is inhibited by mercury ions (PubMed:26416735). Fructose uptake is only slightly inhibited by cytochalasin B (PubMed:8333543, PubMed:9820812).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31Fructose1 Publication1
Binding sitei166Fructose1 Publication1
Binding sitei287Fructose1 Publication1
Binding sitei386Fructose1 Publication1

GO - Molecular functioni

  • fructose binding Source: UniProtKB
  • fructose transmembrane transporter activity Source: UniProtKB
  • glucose transmembrane transporter activity Source: RGD

GO - Biological processi

  • cellular response to fructose stimulus Source: UniProtKB
  • fructose import across plasma membrane Source: UniProtKB
  • fructose transport Source: RGD
  • glucose transport Source: RGD
  • regulation of systemic arterial blood pressure mediated by a chemical signal Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-189200. Hexose transport.
R-RNO-428776. Class II GLUTs.
R-RNO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 2, facilitated glucose transporter member 5
Alternative name(s):
Fructose transporter
Glucose transporter type 5, small intestine
Short name:
GLUT-5
Gene namesi
Name:Slc2a5
Synonyms:Glut5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi68328. Slc2a5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17Cytoplasmic1 PublicationAdd BLAST17
Transmembranei18 – 38Helical; Name=11 PublicationAdd BLAST21
Topological domaini39 – 67Extracellular1 PublicationAdd BLAST29
Transmembranei68 – 90Helical; Name=21 PublicationAdd BLAST23
Topological domaini91 – 97Cytoplasmic1 Publication7
Transmembranei98 – 118Helical; Name=31 PublicationAdd BLAST21
Topological domaini119 – 125Extracellular1 Publication7
Transmembranei126 – 148Helical; Name=41 PublicationAdd BLAST23
Topological domaini149 – 160Cytoplasmic1 PublicationAdd BLAST12
Transmembranei161 – 181Helical; Name=51 PublicationAdd BLAST21
Topological domaini182 – 191Extracellular1 Publication10
Transmembranei192 – 212Helical; Name=61 PublicationAdd BLAST21
Topological domaini213 – 276Cytoplasmic1 PublicationAdd BLAST64
Transmembranei277 – 297Helical; Name=71 PublicationAdd BLAST21
Topological domaini298 – 312Extracellular1 PublicationAdd BLAST15
Transmembranei313 – 333Helical; Name=81 PublicationAdd BLAST21
Topological domaini334 – 341Cytoplasmic1 Publication8
Transmembranei342 – 362Helical; Name=91 PublicationAdd BLAST21
Topological domaini363 – 370Extracellular1 Publication8
Transmembranei371 – 393Helical; Name=101 PublicationAdd BLAST23
Topological domaini394 – 411Cytoplasmic1 PublicationAdd BLAST18
Transmembranei412 – 432Helical; Name=111 PublicationAdd BLAST21
Topological domaini433 – 438Extracellular1 Publication6
Transmembranei439 – 459Helical; Name=121 PublicationAdd BLAST21
Topological domaini460 – 502Cytoplasmic1 PublicationAdd BLAST43

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: RGD
  • sarcolemma Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166Q → E: Impairs fructose binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000503721 – 502Solute carrier family 2, facilitated glucose transporter member 5Add BLAST502

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Glycosylationi50N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

PaxDbiP43427.
PRIDEiP43427.

Expressioni

Tissue specificityi

Detected in jejunum (PubMed:8404647, PubMed:9820812). Detected in kidney, skeletal muscle, brain and adipose tissue (at protein level) (PubMed:9820812). Detected in small intestine and in kidney, and at much lower levels in brain. Detected in enterocytes in duodenum, jejunum, and ileum (PubMed:8333543).3 Publications

Developmental stagei

Detected in small intestine during embryogenesis, but expression is much higher in adult.1 Publication

Inductioni

Up-regulated in jejunum by a diet with a high fructose content (at protein level) (PubMed:8404647, PubMed:9820812). Up-regulated in kidney by a diet with a high fructose content (at protein level) (PubMed:9820812). Up-regulated in jejunum by a diet with a high fructose content (PubMed:8404647).2 Publications

Gene expression databases

BgeeiENSRNOG00000017693.
ExpressionAtlasiP43427. baseline and differential.
GenevisibleiP43427. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024054.

Structurei

Secondary structure

1502
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 37Combined sources24
Turni41 – 43Combined sources3
Helixi62 – 86Combined sources25
Helixi88 – 120Combined sources33
Helixi124 – 152Combined sources29
Helixi155 – 186Combined sources32
Helixi191 – 208Combined sources18
Helixi209 – 211Combined sources3
Helixi216 – 220Combined sources5
Helixi226 – 237Combined sources12
Beta strandi238 – 240Combined sources3
Helixi243 – 257Combined sources15
Helixi264 – 269Combined sources6
Helixi274 – 288Combined sources15
Helixi292 – 306Combined sources15
Helixi310 – 338Combined sources29
Helixi340 – 367Combined sources28
Helixi371 – 388Combined sources18
Turni389 – 392Combined sources4
Helixi393 – 401Combined sources9
Turni404 – 406Combined sources3
Helixi407 – 436Combined sources30
Helixi437 – 439Combined sources3
Helixi440 – 457Combined sources18
Helixi467 – 479Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YBQX-ray3.27A/B1-502[»]
ProteinModelPortaliP43427.
SMRiP43427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni295 – 297Fructose binding1 Publication3
Regioni418 – 419Fructose binding1 Publication2

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0569. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000119022.
HOGENOMiHOG000202871.
HOVERGENiHBG014816.
InParanoidiP43427.
KOiK08143.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR002442. Fru_transpt_5.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR01194. GLUCTRSPORT5.
PR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKEDQEKTG KLTLVLALAT FLAAFGSSFQ YGYNVAAVNS PSEFMQQFYN
60 70 80 90 100
DTYYDRNKEN IESFTLTLLW SLTVSMFPFG GFIGSLMVGF LVNNLGRKGA
110 120 130 140 150
LLFNNIFSIL PAILMGCSKI AKSFEIIIAS RLLVGICAGI SSNVVPMYLG
160 170 180 190 200
ELAPKNLRGA LGVVPQLFIT VGILVAQLFG LRSVLASEEG WPILLGLTGV
210 220 230 240 250
PAGLQLLLLP FFPESPRYLL IQKKNESAAE KALQTLRGWK DVDMEMEEIR
260 270 280 290 300
KEDEAEKAAG FISVWKLFRM QSLRWQLIST IVLMAGQQLS GVNAIYYYAD
310 320 330 340 350
QIYLSAGVKS NDVQYVTAGT GAVNVFMTMV TVFVVELWGR RNLLLIGFST
360 370 380 390 400
CLTACIVLTV ALALQNTISW MPYVSIVCVI VYVIGHAVGP SPIPALFITE
410 420 430 440 450
IFLQSSRPSA YMIGGSVHWL SNFIVGLIFP FIQVGLGPYS FIIFAIICLL
460 470 480 490 500
TTIYIFMVVP ETKGRTFVEI NQIFAKKNKV SDVYPEKEEK ELNDLPPATR

EQ
Length:502
Mass (Da):55,543
Last modified:November 1, 1995 - v1
Checksum:i871FEDEFA1E7884D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164V → A in AAA02627 (PubMed:8333543).Curated1
Sequence conflicti285A → T in BAA02983 (PubMed:8404647).Curated1
Sequence conflicti452T → S in BAA02983 (PubMed:8404647).Curated1
Sequence conflicti479K → N in AAA02627 (PubMed:8333543).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05195 mRNA. Translation: AAA02627.1.
D13871 mRNA. Translation: BAA02983.1.
D28562 mRNA. Translation: BAA05912.1.
BC076378 mRNA. Translation: AAH76378.1.
PIRiI53268.
RefSeqiNP_113929.1. NM_031741.1.
UniGeneiRn.10900.

Genome annotation databases

EnsembliENSRNOT00000024054; ENSRNOP00000024054; ENSRNOG00000017693.
GeneIDi65197.
KEGGirno:65197.
UCSCiRGD:68328. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05195 mRNA. Translation: AAA02627.1.
D13871 mRNA. Translation: BAA02983.1.
D28562 mRNA. Translation: BAA05912.1.
BC076378 mRNA. Translation: AAH76378.1.
PIRiI53268.
RefSeqiNP_113929.1. NM_031741.1.
UniGeneiRn.10900.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YBQX-ray3.27A/B1-502[»]
ProteinModelPortaliP43427.
SMRiP43427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024054.

Proteomic databases

PaxDbiP43427.
PRIDEiP43427.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024054; ENSRNOP00000024054; ENSRNOG00000017693.
GeneIDi65197.
KEGGirno:65197.
UCSCiRGD:68328. rat.

Organism-specific databases

CTDi6518.
RGDi68328. Slc2a5.

Phylogenomic databases

eggNOGiKOG0569. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000119022.
HOGENOMiHOG000202871.
HOVERGENiHBG014816.
InParanoidiP43427.
KOiK08143.

Enzyme and pathway databases

ReactomeiR-RNO-189200. Hexose transport.
R-RNO-428776. Class II GLUTs.
R-RNO-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP43427.

Gene expression databases

BgeeiENSRNOG00000017693.
ExpressionAtlasiP43427. baseline and differential.
GenevisibleiP43427. RN.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR002442. Fru_transpt_5.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR01194. GLUCTRSPORT5.
PR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGTR5_RAT
AccessioniPrimary (citable) accession number: P43427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.