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Protein

Solute carrier family 2, facilitated glucose transporter member 5

Gene

Slc2a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a fructose transporter that has only low activity with other monosaccharides (PubMed:8333543, PubMed:9820812, PubMed:26416735). Can mediate the uptake of deoxyglucose, but with low efficiency (PubMed:8333543). Essential for fructose uptake in the small intestine. Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose. Required for the development of high blood pressure in response to high dietary fructose intake.By similarity3 Publications

Enzyme regulationi

Fructose uptake is inhibited by mercury ions (PubMed:26416735). Fructose uptake is only slightly inhibited by cytochalasin B (PubMed:8333543, PubMed:9820812).3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311Fructose1 Publication
Binding sitei166 – 1661Fructose1 Publication
Binding sitei287 – 2871Fructose1 Publication
Binding sitei386 – 3861Fructose1 Publication

GO - Molecular functioni

  • fructose binding Source: UniProtKB
  • fructose transmembrane transporter activity Source: UniProtKB
  • glucose transmembrane transporter activity Source: RGD

GO - Biological processi

  • cellular response to fructose stimulus Source: UniProtKB
  • fructose import across plasma membrane Source: UniProtKB
  • fructose transport Source: RGD
  • glucose transport Source: RGD
  • regulation of systemic arterial blood pressure mediated by a chemical signal Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-189200. Hexose transport.
R-RNO-428776. Class II GLUTs.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 2, facilitated glucose transporter member 5
Alternative name(s):
Fructose transporter
Glucose transporter type 5, small intestine
Short name:
GLUT-5
Gene namesi
Name:Slc2a5
Synonyms:Glut5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi68328. Slc2a5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1717Cytoplasmic1 PublicationAdd
BLAST
Transmembranei18 – 3821Helical; Name=11 PublicationAdd
BLAST
Topological domaini39 – 6729Extracellular1 PublicationAdd
BLAST
Transmembranei68 – 9023Helical; Name=21 PublicationAdd
BLAST
Topological domaini91 – 977Cytoplasmic1 Publication
Transmembranei98 – 11821Helical; Name=31 PublicationAdd
BLAST
Topological domaini119 – 1257Extracellular1 Publication
Transmembranei126 – 14823Helical; Name=41 PublicationAdd
BLAST
Topological domaini149 – 16012Cytoplasmic1 PublicationAdd
BLAST
Transmembranei161 – 18121Helical; Name=51 PublicationAdd
BLAST
Topological domaini182 – 19110Extracellular1 Publication
Transmembranei192 – 21221Helical; Name=61 PublicationAdd
BLAST
Topological domaini213 – 27664Cytoplasmic1 PublicationAdd
BLAST
Transmembranei277 – 29721Helical; Name=71 PublicationAdd
BLAST
Topological domaini298 – 31215Extracellular1 PublicationAdd
BLAST
Transmembranei313 – 33321Helical; Name=81 PublicationAdd
BLAST
Topological domaini334 – 3418Cytoplasmic1 Publication
Transmembranei342 – 36221Helical; Name=91 PublicationAdd
BLAST
Topological domaini363 – 3708Extracellular1 Publication
Transmembranei371 – 39323Helical; Name=101 PublicationAdd
BLAST
Topological domaini394 – 41118Cytoplasmic1 PublicationAdd
BLAST
Transmembranei412 – 43221Helical; Name=111 PublicationAdd
BLAST
Topological domaini433 – 4386Extracellular1 Publication
Transmembranei439 – 45921Helical; Name=121 PublicationAdd
BLAST
Topological domaini460 – 50243Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: RGD
  • sarcolemma Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi166 – 1661Q → E: Impairs fructose binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Solute carrier family 2, facilitated glucose transporter member 5PRO_0000050372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

PaxDbiP43427.
PRIDEiP43427.

Expressioni

Tissue specificityi

Detected in jejunum (PubMed:8404647, PubMed:9820812). Detected in kidney, skeletal muscle, brain and adipose tissue (at protein level) (PubMed:9820812). Detected in small intestine and in kidney, and at much lower levels in brain. Detected in enterocytes in duodenum, jejunum, and ileum (PubMed:8333543).3 Publications

Developmental stagei

Detected in small intestine during embryogenesis, but expression is much higher in adult.1 Publication

Inductioni

Up-regulated in jejunum by a diet with a high fructose content (at protein level) (PubMed:8404647, PubMed:9820812). Up-regulated in kidney by a diet with a high fructose content (at protein level) (PubMed:9820812). Up-regulated in jejunum by a diet with a high fructose content (PubMed:8404647).2 Publications

Gene expression databases

ExpressionAtlasiP43427. baseline and differential.
GenevisibleiP43427. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024054.

Structurei

Secondary structure

1
502
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 3724Combined sources
Turni41 – 433Combined sources
Helixi62 – 8625Combined sources
Helixi88 – 12033Combined sources
Helixi124 – 15229Combined sources
Helixi155 – 18632Combined sources
Helixi191 – 20818Combined sources
Helixi209 – 2113Combined sources
Helixi216 – 2205Combined sources
Helixi226 – 23712Combined sources
Beta strandi238 – 2403Combined sources
Helixi243 – 25715Combined sources
Helixi264 – 2696Combined sources
Helixi274 – 28815Combined sources
Helixi292 – 30615Combined sources
Helixi310 – 33829Combined sources
Helixi340 – 36728Combined sources
Helixi371 – 38818Combined sources
Turni389 – 3924Combined sources
Helixi393 – 4019Combined sources
Turni404 – 4063Combined sources
Helixi407 – 43630Combined sources
Helixi437 – 4393Combined sources
Helixi440 – 45718Combined sources
Helixi467 – 47913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YBQX-ray3.27A/B1-502[»]
ProteinModelPortaliP43427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni295 – 2973Fructose binding1 Publication
Regioni418 – 4192Fructose binding1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0569. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000119022.
HOGENOMiHOG000202871.
HOVERGENiHBG014816.
InParanoidiP43427.
KOiK08143.
OMAiNTISWMP.
OrthoDBiEOG7QVM2R.

Family and domain databases

InterProiIPR002442. Fru_transpt_5.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR01194. GLUCTRSPORT5.
PR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKEDQEKTG KLTLVLALAT FLAAFGSSFQ YGYNVAAVNS PSEFMQQFYN
60 70 80 90 100
DTYYDRNKEN IESFTLTLLW SLTVSMFPFG GFIGSLMVGF LVNNLGRKGA
110 120 130 140 150
LLFNNIFSIL PAILMGCSKI AKSFEIIIAS RLLVGICAGI SSNVVPMYLG
160 170 180 190 200
ELAPKNLRGA LGVVPQLFIT VGILVAQLFG LRSVLASEEG WPILLGLTGV
210 220 230 240 250
PAGLQLLLLP FFPESPRYLL IQKKNESAAE KALQTLRGWK DVDMEMEEIR
260 270 280 290 300
KEDEAEKAAG FISVWKLFRM QSLRWQLIST IVLMAGQQLS GVNAIYYYAD
310 320 330 340 350
QIYLSAGVKS NDVQYVTAGT GAVNVFMTMV TVFVVELWGR RNLLLIGFST
360 370 380 390 400
CLTACIVLTV ALALQNTISW MPYVSIVCVI VYVIGHAVGP SPIPALFITE
410 420 430 440 450
IFLQSSRPSA YMIGGSVHWL SNFIVGLIFP FIQVGLGPYS FIIFAIICLL
460 470 480 490 500
TTIYIFMVVP ETKGRTFVEI NQIFAKKNKV SDVYPEKEEK ELNDLPPATR

EQ
Length:502
Mass (Da):55,543
Last modified:November 1, 1995 - v1
Checksum:i871FEDEFA1E7884D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641V → A in AAA02627 (PubMed:8333543).Curated
Sequence conflicti285 – 2851A → T in BAA02983 (PubMed:8404647).Curated
Sequence conflicti452 – 4521T → S in BAA02983 (PubMed:8404647).Curated
Sequence conflicti479 – 4791K → N in AAA02627 (PubMed:8333543).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05195 mRNA. Translation: AAA02627.1.
D13871 mRNA. Translation: BAA02983.1.
D28562 mRNA. Translation: BAA05912.1.
BC076378 mRNA. Translation: AAH76378.1.
PIRiI53268.
RefSeqiNP_113929.1. NM_031741.1.
UniGeneiRn.10900.

Genome annotation databases

EnsembliENSRNOT00000024054; ENSRNOP00000024054; ENSRNOG00000017693.
GeneIDi65197.
KEGGirno:65197.
UCSCiRGD:68328. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05195 mRNA. Translation: AAA02627.1.
D13871 mRNA. Translation: BAA02983.1.
D28562 mRNA. Translation: BAA05912.1.
BC076378 mRNA. Translation: AAH76378.1.
PIRiI53268.
RefSeqiNP_113929.1. NM_031741.1.
UniGeneiRn.10900.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YBQX-ray3.27A/B1-502[»]
ProteinModelPortaliP43427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024054.

Proteomic databases

PaxDbiP43427.
PRIDEiP43427.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024054; ENSRNOP00000024054; ENSRNOG00000017693.
GeneIDi65197.
KEGGirno:65197.
UCSCiRGD:68328. rat.

Organism-specific databases

CTDi6518.
RGDi68328. Slc2a5.

Phylogenomic databases

eggNOGiKOG0569. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000119022.
HOGENOMiHOG000202871.
HOVERGENiHBG014816.
InParanoidiP43427.
KOiK08143.
OMAiNTISWMP.
OrthoDBiEOG7QVM2R.

Enzyme and pathway databases

ReactomeiR-RNO-189200. Hexose transport.
R-RNO-428776. Class II GLUTs.

Miscellaneous databases

PROiP43427.

Gene expression databases

ExpressionAtlasiP43427. baseline and differential.
GenevisibleiP43427. RN.

Family and domain databases

InterProiIPR002442. Fru_transpt_5.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR01194. GLUCTRSPORT5.
PR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, tissue distribution, and functional characterization of the rat fructose transporter GLUT5."
    Rand E.B., Depaoli A.M., Davidson N.O., Bell G.I., Burant C.F.
    Am. J. Physiol. 264:G1169-G1176(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, ENZYME REGULATION.
    Strain: Sprague-Dawley.
    Tissue: Jejunum.
  2. "Cloning and increased expression with fructose feeding of rat jejunal GLUT5."
    Inukai K., Asano T., Katagiri H., Ishihara H., Anai M., Fukushima Y., Tsukuda K., Kikuchi M., Yazaki Y., Oka Y.
    Endocrinology 133:2009-2014(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY FRUCTOSE.
    Strain: Sprague-Dawley.
    Tissue: Jejunum.
  3. Kasahara T., Kasahara M.
    Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Intestine.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  5. "Biochemical and functional characterization of the GLUT5 fructose transporter in rat skeletal muscle."
    Darakhshan F., Hajduch E., Kristiansen S., Richter E.A., Hundal H.S.
    Biochem. J. 336:361-366(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION BY FRUCTOSE.
  6. Cited for: X-RAY CRYSTALLOGRAPHY (3.27 ANGSTROMS), FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, ENZYME REGULATION, MUTAGENESIS OF GLN-166.

Entry informationi

Entry nameiGTR5_RAT
AccessioniPrimary (citable) accession number: P43427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.