Reviewed, UniProtKB/Swiss-Prot P43424 (GALT_RAT)

Names and origin

Protein names

Recommended name:
    Galactose-1-phosphate uridylyltransferase
      Short name=Gal-1-P uridylyltransferase
    EC=2.7.7.12
Alternative name(s):
    UDP-glucose--hexose-1-phosphate uridylyltransferase

Gene names

Name:

Galt

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	UDP-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-galactose.
Cofactor	Binds 1 iron ion per subunit Potential. Binds 1 zinc ion per subunit Potential.
Pathway	Carbohydrate metabolism; galactose metabolism.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Ligand	Iron Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	UDP-glucose conversion Inferred from direct assay. Source: RGD galactose metabolic process Inferred from direct assay. Source: RGD
Cellular component	soluble fraction Inferred from direct assay. Source: RGD
Molecular function	UDP-glucose:hexose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: RGD galactose binding Inferred by curator. Source: RGD glucose binding Inferred by curator. Source: RGD iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Galactose-1-phosphate uridylyltransferase		PRO_0000169884
Sites
	Active site	186	1	Tele-UMP-histidine intermediate By similarity
	Metal binding	75	1	Zinc Potential
	Metal binding	184	1	Zinc Potential
	Metal binding	202	1	Iron Potential
	Metal binding	301	1	Iron Potential
	Metal binding	319	1	Iron Potential
	Metal binding	321	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	P43424-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: F217FA0A3A3E4665	FASTA	379	43,314
	10 20 30 40 50 60 MSQSGADPEQ RQQASEADAM AATFRASEHQ HIRYNPLQDE WVLVSAHRMK RPWQGQVEPQ 70 80 90 100 110 120 LLKTVPRHDP LNPLCPGATR ANGEVNPPYD GTFLFDNDFP ALQPDAPDPG PSDHPLFRVE 130 140 150 160 170 180 AARGVCKVMC FHPWSDVTLP LMSVPEIRAV IDAWASVTEE LGAQYPWVQI FENKGAMMGC 190 200 210 220 230 240 SNPHPHCQVW ASNFLPDIAQ REERSQQTYH NQHGKPLLLE YGHQELLRKE RLVLTSEYWI 250 260 270 280 290 300 VLVPFWAVWP FQTLLLPRRH VQRLPELTPA ERDDLASTMK KLLTKYDNLF ETSFPYSMGW 310 320 330 340 350 360 HGAPMGLKTG ATCDHWQLHA HYYPPLLRSA TVRKFMVGYE MLAQAQRDLT PEQAAERLRV 370 LPEVHYCLTQ KDKETAATA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat galactose-1-phosphate uridyltransferase coding sequence, transcription start site and genomic organization." Heidenreich R.A., Mallee J., Segal S. DNA Seq. 3:311-318(1993) [PubMed: 8400361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L05541 Genomic DNA. Translation: AAC37609.1. BC098756 mRNA. Translation: AAH98756.1.
IPI	IPI00555211.
PIR	A56685.
RefSeq	NP_001013107.1.
UniGene	Rn.102781
3D structure databases
HSSP	HSSP built from PDB template 1HXQ based on UniProtKB P09148.
ModBase	Search...
Protein-protein interaction databases
STRING	P43424.
Proteomic databases
PRIDE	P43424.
Genome annotation databases
Ensembl	ENSRNOT00000019886; ENSRNOP00000019886; ENSRNOG00000014766; Rattus norvegicus. [Genome view]
GeneID	298003.
KEGG	rno:298003.
UCSC	NM_001013089. rat.
Organism-specific databases
CTD	298003.
RGD	1306483. Galt.
Phylogenomic databases
HOVERGEN	P43424.
OMA	DHWQLHA.
Enzyme and pathway databases
BRENDA	2.7.7.12. 248.
Gene expression databases
ArrayExpress	P43424.
Genevestigator	P43424.
GermOnline	ENSRNOG00000014766. Rattus norvegicus.
Family and domain databases
InterPro	IPR001937. GalP_UDPtransf1. IPR019779. GalP_UDPtransf1_His-AS. IPR005850. GalP_Utransf_C. IPR005849. GalP_Utransf_N. IPR011151. His_triad_motif. [Graphical view]
Gene3D	G3DSA:3.30.428.10. His_triad_motif. 2 hits.
PANTHER	PTHR11943. GalP_UDPtransf1. 1 hit.
Pfam	PF02744. GalP_UDP_tr_C. 1 hit. PF01087. GalP_UDP_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000808. GalT. 1 hit.
ProDom	PD005051. GalP_Utransf. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00209. galT_1. 1 hit.
PROSITE	PS00117. GAL_P_UDP_TRANSF_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	642951.

Entry information

Entry name

GALT_RAT

Accession

Primary (citable) accession number: P43424
Secondary accession number(s): Q4KM61

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)