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Protein

Modification methylase BseCI

Gene

bseCIM

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.72. 623.

Protein family/group databases

REBASEi3536. M.BseCI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BseCI (EC:2.1.1.72)
Short name:
M.BseCI
Alternative name(s):
Adenine-specific methyltransferase BseCI
Gene namesi
Name:bseCIM
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 579579Modification methylase BseCIPRO_0000087944Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP43423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSVQKANTV SRQKATGAHF TPDKLAEVIA KRILDYFKGE KNRVIRVLDP
60 70 80 90 100
ACGDGELLLA INKVAQSMNI QLELIGVDFD IDAINIANER LSRSGHKNFR
110 120 130 140 150
LINKDFLEMV SEGDNYDLFN IEELEPVDII IANPPYVRTQ ILGAEKAQKL
160 170 180 190 200
REKFNLKGRV DLYQAFLVAM TQQLKSNGII GVITSNRYLT TKGGGSTRKF
210 220 230 240 250
LVSNFNILEI MDLGDSKFFE AAVLPAIFFG EKKNKEYQKE NSNVPKFFKI
260 270 280 290 300
YEQSDIEASS SVNSEFNSLI ELLEVNKSGL YSVEDKTYSI SLGKIISPEN
310 320 330 340 350
YKEPWILATE DEYEWFMKVN QNAYGFIEDF AHVKVGIKTT ADSVFIRSDW
360 370 380 390 400
GELPEEQIPE DKLLRPIISA DQANKWSVSL VGNNKKVLYT HEIRDGQIKA
410 420 430 440 450
INLEEFPRAK NYLESHKERL ASRKYVLKAN RNWYEIWVPH DPSLWDKPKI
460 470 480 490 500
IFPDTSPEPK FFYEDKGSVV DGNCYWIIPK KENSNDILFL IMGICNSKFM
510 520 530 540 550
SKYHDIAFQN KLYAGRRRYL TQYVNKYPIP DPESIYSKEI ISLVRELVNN
560 570
KKETQDINEI ENRIEKLILR AFDIESLKY
Length:579
Mass (Da):66,774
Last modified:October 31, 1995 - v1
Checksum:iA4805FBEBA01835C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79509 Genomic DNA. Translation: CAA56041.1.
PIRiI40371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79509 Genomic DNA. Translation: CAA56041.1.
PIRiI40371.

3D structure databases

ProteinModelPortaliP43423.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3536. M.BseCI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.1.1.72. 623.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of the cloned bseCIM gene: M.BseCI reveals high homology to M.BanIII."
    Rina M., Markaki M., Bouriotis V.
    Gene 150:71-73(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTC1_GEOSE
AccessioniPrimary (citable) accession number: P43423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1995
Last sequence update: October 31, 1995
Last modified: January 6, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.