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P43423 (MTC1_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BseCI

Short name=M.BseCI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase BseCI
Gene names
Name:bseCIM
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length579 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 579579Modification methylase BseCI
PRO_0000087944

Sequences

Sequence LengthMass (Da)Tools
P43423 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A4805FBEBA01835C

FASTA57966,774
        10         20         30         40         50         60 
MMSVQKANTV SRQKATGAHF TPDKLAEVIA KRILDYFKGE KNRVIRVLDP ACGDGELLLA 

        70         80         90        100        110        120 
INKVAQSMNI QLELIGVDFD IDAINIANER LSRSGHKNFR LINKDFLEMV SEGDNYDLFN 

       130        140        150        160        170        180 
IEELEPVDII IANPPYVRTQ ILGAEKAQKL REKFNLKGRV DLYQAFLVAM TQQLKSNGII 

       190        200        210        220        230        240 
GVITSNRYLT TKGGGSTRKF LVSNFNILEI MDLGDSKFFE AAVLPAIFFG EKKNKEYQKE 

       250        260        270        280        290        300 
NSNVPKFFKI YEQSDIEASS SVNSEFNSLI ELLEVNKSGL YSVEDKTYSI SLGKIISPEN 

       310        320        330        340        350        360 
YKEPWILATE DEYEWFMKVN QNAYGFIEDF AHVKVGIKTT ADSVFIRSDW GELPEEQIPE 

       370        380        390        400        410        420 
DKLLRPIISA DQANKWSVSL VGNNKKVLYT HEIRDGQIKA INLEEFPRAK NYLESHKERL 

       430        440        450        460        470        480 
ASRKYVLKAN RNWYEIWVPH DPSLWDKPKI IFPDTSPEPK FFYEDKGSVV DGNCYWIIPK 

       490        500        510        520        530        540 
KENSNDILFL IMGICNSKFM SKYHDIAFQN KLYAGRRRYL TQYVNKYPIP DPESIYSKEI 

       550        560        570 
ISLVRELVNN KKETQDINEI ENRIEKLILR AFDIESLKY 

« Hide

References

[1]"Sequence of the cloned bseCIM gene: M.BseCI reveals high homology to M.BanIII."
Rina M., Markaki M., Bouriotis V.
Gene 150:71-73(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79509 Genomic DNA. Translation: CAA56041.1.
PIRI40371.

3D structure databases

ProteinModelPortalP43423.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3536. M.BseCI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.1.1.72. 623.

Family and domain databases

InterProIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR025931. TaqI_C.
[Graphical view]
PfamPF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTC1_GEOSE
AccessionPrimary (citable) accession number: P43423
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries