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P43423

- MTC1_GEOSE

UniProt

P43423 - MTC1_GEOSE

Protein

Modification methylase BseCI

Gene

bseCIM

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BRENDAi2.1.1.72. 623.

    Protein family/group databases

    REBASEi3536. M.BseCI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase BseCI (EC:2.1.1.72)
    Short name:
    M.BseCI
    Alternative name(s):
    Adenine-specific methyltransferase BseCI
    Gene namesi
    Name:bseCIM
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 579579Modification methylase BseCIPRO_0000087944Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP43423.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view]
    PfamiPF02384. N6_Mtase. 1 hit.
    PF12950. TaqI_C. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43423-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMSVQKANTV SRQKATGAHF TPDKLAEVIA KRILDYFKGE KNRVIRVLDP    50
    ACGDGELLLA INKVAQSMNI QLELIGVDFD IDAINIANER LSRSGHKNFR 100
    LINKDFLEMV SEGDNYDLFN IEELEPVDII IANPPYVRTQ ILGAEKAQKL 150
    REKFNLKGRV DLYQAFLVAM TQQLKSNGII GVITSNRYLT TKGGGSTRKF 200
    LVSNFNILEI MDLGDSKFFE AAVLPAIFFG EKKNKEYQKE NSNVPKFFKI 250
    YEQSDIEASS SVNSEFNSLI ELLEVNKSGL YSVEDKTYSI SLGKIISPEN 300
    YKEPWILATE DEYEWFMKVN QNAYGFIEDF AHVKVGIKTT ADSVFIRSDW 350
    GELPEEQIPE DKLLRPIISA DQANKWSVSL VGNNKKVLYT HEIRDGQIKA 400
    INLEEFPRAK NYLESHKERL ASRKYVLKAN RNWYEIWVPH DPSLWDKPKI 450
    IFPDTSPEPK FFYEDKGSVV DGNCYWIIPK KENSNDILFL IMGICNSKFM 500
    SKYHDIAFQN KLYAGRRRYL TQYVNKYPIP DPESIYSKEI ISLVRELVNN 550
    KKETQDINEI ENRIEKLILR AFDIESLKY 579
    Length:579
    Mass (Da):66,774
    Last modified:November 1, 1995 - v1
    Checksum:iA4805FBEBA01835C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79509 Genomic DNA. Translation: CAA56041.1.
    PIRiI40371.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79509 Genomic DNA. Translation: CAA56041.1 .
    PIRi I40371.

    3D structure databases

    ProteinModelPortali P43423.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3536. M.BseCI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.1.1.72. 623.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view ]
    Pfami PF02384. N6_Mtase. 1 hit.
    PF12950. TaqI_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the cloned bseCIM gene: M.BseCI reveals high homology to M.BanIII."
      Rina M., Markaki M., Bouriotis V.
      Gene 150:71-73(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiMTC1_GEOSE
    AccessioniPrimary (citable) accession number: P43423
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3