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P43423

- MTC1_GEOSE

UniProt

P43423 - MTC1_GEOSE

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Protein
Modification methylase BseCI
Gene
bseCIM
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.72. 623.

Protein family/group databases

REBASEi3536. M.BseCI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BseCI (EC:2.1.1.72)
Short name:
M.BseCI
Alternative name(s):
Adenine-specific methyltransferase BseCI
Gene namesi
Name:bseCIM
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 579579Modification methylase BseCI
PRO_0000087944Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP43423.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43423-1 [UniParc]FASTAAdd to Basket

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MMSVQKANTV SRQKATGAHF TPDKLAEVIA KRILDYFKGE KNRVIRVLDP    50
ACGDGELLLA INKVAQSMNI QLELIGVDFD IDAINIANER LSRSGHKNFR 100
LINKDFLEMV SEGDNYDLFN IEELEPVDII IANPPYVRTQ ILGAEKAQKL 150
REKFNLKGRV DLYQAFLVAM TQQLKSNGII GVITSNRYLT TKGGGSTRKF 200
LVSNFNILEI MDLGDSKFFE AAVLPAIFFG EKKNKEYQKE NSNVPKFFKI 250
YEQSDIEASS SVNSEFNSLI ELLEVNKSGL YSVEDKTYSI SLGKIISPEN 300
YKEPWILATE DEYEWFMKVN QNAYGFIEDF AHVKVGIKTT ADSVFIRSDW 350
GELPEEQIPE DKLLRPIISA DQANKWSVSL VGNNKKVLYT HEIRDGQIKA 400
INLEEFPRAK NYLESHKERL ASRKYVLKAN RNWYEIWVPH DPSLWDKPKI 450
IFPDTSPEPK FFYEDKGSVV DGNCYWIIPK KENSNDILFL IMGICNSKFM 500
SKYHDIAFQN KLYAGRRRYL TQYVNKYPIP DPESIYSKEI ISLVRELVNN 550
KKETQDINEI ENRIEKLILR AFDIESLKY 579
Length:579
Mass (Da):66,774
Last modified:November 1, 1995 - v1
Checksum:iA4805FBEBA01835C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79509 Genomic DNA. Translation: CAA56041.1.
PIRiI40371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79509 Genomic DNA. Translation: CAA56041.1 .
PIRi I40371.

3D structure databases

ProteinModelPortali P43423.
ModBasei Search...

Protein family/group databases

REBASEi 3536. M.BseCI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.1.1.72. 623.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
IPR025931. TaqI_C.
[Graphical view ]
Pfami PF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the cloned bseCIM gene: M.BseCI reveals high homology to M.BanIII."
    Rina M., Markaki M., Bouriotis V.
    Gene 150:71-73(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTC1_GEOSE
AccessioniPrimary (citable) accession number: P43423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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