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P43423

- MTC1_GEOSE

UniProt

P43423 - MTC1_GEOSE

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Protein

Modification methylase BseCI

Gene

bseCIM

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.72. 623.

Protein family/group databases

REBASEi3536. M.BseCI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BseCI (EC:2.1.1.72)
Short name:
M.BseCI
Alternative name(s):
Adenine-specific methyltransferase BseCI
Gene namesi
Name:bseCIM
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 579579Modification methylase BseCIPRO_0000087944Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP43423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43423-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMSVQKANTV SRQKATGAHF TPDKLAEVIA KRILDYFKGE KNRVIRVLDP
60 70 80 90 100
ACGDGELLLA INKVAQSMNI QLELIGVDFD IDAINIANER LSRSGHKNFR
110 120 130 140 150
LINKDFLEMV SEGDNYDLFN IEELEPVDII IANPPYVRTQ ILGAEKAQKL
160 170 180 190 200
REKFNLKGRV DLYQAFLVAM TQQLKSNGII GVITSNRYLT TKGGGSTRKF
210 220 230 240 250
LVSNFNILEI MDLGDSKFFE AAVLPAIFFG EKKNKEYQKE NSNVPKFFKI
260 270 280 290 300
YEQSDIEASS SVNSEFNSLI ELLEVNKSGL YSVEDKTYSI SLGKIISPEN
310 320 330 340 350
YKEPWILATE DEYEWFMKVN QNAYGFIEDF AHVKVGIKTT ADSVFIRSDW
360 370 380 390 400
GELPEEQIPE DKLLRPIISA DQANKWSVSL VGNNKKVLYT HEIRDGQIKA
410 420 430 440 450
INLEEFPRAK NYLESHKERL ASRKYVLKAN RNWYEIWVPH DPSLWDKPKI
460 470 480 490 500
IFPDTSPEPK FFYEDKGSVV DGNCYWIIPK KENSNDILFL IMGICNSKFM
510 520 530 540 550
SKYHDIAFQN KLYAGRRRYL TQYVNKYPIP DPESIYSKEI ISLVRELVNN
560 570
KKETQDINEI ENRIEKLILR AFDIESLKY
Length:579
Mass (Da):66,774
Last modified:November 1, 1995 - v1
Checksum:iA4805FBEBA01835C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79509 Genomic DNA. Translation: CAA56041.1.
PIRiI40371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79509 Genomic DNA. Translation: CAA56041.1 .
PIRi I40371.

3D structure databases

ProteinModelPortali P43423.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3536. M.BseCI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.1.1.72. 623.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
IPR025931. TaqI_C.
[Graphical view ]
Pfami PF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the cloned bseCIM gene: M.BseCI reveals high homology to M.BanIII."
    Rina M., Markaki M., Bouriotis V.
    Gene 150:71-73(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTC1_GEOSE
AccessioniPrimary (citable) accession number: P43423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 1, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3