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P43422 (MTV1_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BstVI

Short name=M.BstVI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase BstVI
Gene names
Name:bstVIM
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BstVI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 561561Modification methylase BstVI
PRO_0000087945

Sequences

Sequence LengthMass (Da)Tools
P43422 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 4D463062E4941859

FASTA56165,702
        10         20         30         40         50         60 
MVTQHSYRMH GAVYTKPIIV DLILDLTGYT SDKNLENFKL LDPSFGDGVF LEAAVHRLMD 

        70         80         90        100        110        120 
SLIRRGYRPN ELIDHLGNCI RGIELRLEAY QAGRHRLQKV LEGYGFSKPE INWLINQWII 

       130        140        150        160        170        180 
QADFLLWQED TTEAIKFDFV VGNPPYVRQE LIQDELIKKY RKRYTTIYDR ADLYVPFIQH 

       190        200        210        220        230        240 
SLELLSEQGT LGIICSDRFT KNRYGKKLRK FITDNYKVRY IVDLHKTSPF ENEVTAYPAI 

       250        260        270        280        290        300 
YVIKTKNYDK SVVRAVYTEV ITSKVCQDAK DFLLSNQKPD QSSKEMKTYV FSEWFAGDEP 

       310        320        330        340        350        360 
WIIQSQECRE ILRRLENRFP LIEDDVHSCK IRIGVATGAD KVYIVDPQQV DIEPEVLLPL 

       370        380        390        400        410        420 
VTTADISSGR IIWSGKHVIN PFNSDGGLIN LDDFPRLKTY FQQHEEIIKN RNVAKKNPSQ 

       430        440        450        460        470        480 
WFRTIDRIYP EIVHQPKLLI PDMKNTNHIV KDDGAFYPHH NLYYILPGNW NIDILRAILL 

       490        500        510        520        530        540 
SSVVKFFIWS YATKMRGDTL RYQAQYLRKI RLPDPKSLTN DQKERLMDER VIQSQEYLDS 

       550        560 
IVAEIYQLSK TEIEIIKDAL E 

« Hide

References

[1]"Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system."
Gonzalez E., Vasquez C.
Gene 131:103-106(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: V.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07642 Genomic DNA. Translation: AAA51408.1.
PIRJN0797.

3D structure databases

ProteinModelPortalP43422.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3331. M.BstVI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR025931. TaqI_C.
[Graphical view]
PfamPF07669. Eco57I. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTV1_GEOSE
AccessionPrimary (citable) accession number: P43422
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries