Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P43422

- MTV1_GEOSE

UniProt

P43422 - MTV1_GEOSE

Protein

Modification methylase BstVI

Gene

bstVIM

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BstVI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3331. M.BstVI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase BstVI (EC:2.1.1.72)
    Short name:
    M.BstVI
    Alternative name(s):
    Adenine-specific methyltransferase BstVI
    Gene namesi
    Name:bstVIM
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 561561Modification methylase BstVIPRO_0000087945Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP43422.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view]
    PfamiPF07669. Eco57I. 1 hit.
    PF12950. TaqI_C. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43422-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTQHSYRMH GAVYTKPIIV DLILDLTGYT SDKNLENFKL LDPSFGDGVF    50
    LEAAVHRLMD SLIRRGYRPN ELIDHLGNCI RGIELRLEAY QAGRHRLQKV 100
    LEGYGFSKPE INWLINQWII QADFLLWQED TTEAIKFDFV VGNPPYVRQE 150
    LIQDELIKKY RKRYTTIYDR ADLYVPFIQH SLELLSEQGT LGIICSDRFT 200
    KNRYGKKLRK FITDNYKVRY IVDLHKTSPF ENEVTAYPAI YVIKTKNYDK 250
    SVVRAVYTEV ITSKVCQDAK DFLLSNQKPD QSSKEMKTYV FSEWFAGDEP 300
    WIIQSQECRE ILRRLENRFP LIEDDVHSCK IRIGVATGAD KVYIVDPQQV 350
    DIEPEVLLPL VTTADISSGR IIWSGKHVIN PFNSDGGLIN LDDFPRLKTY 400
    FQQHEEIIKN RNVAKKNPSQ WFRTIDRIYP EIVHQPKLLI PDMKNTNHIV 450
    KDDGAFYPHH NLYYILPGNW NIDILRAILL SSVVKFFIWS YATKMRGDTL 500
    RYQAQYLRKI RLPDPKSLTN DQKERLMDER VIQSQEYLDS IVAEIYQLSK 550
    TEIEIIKDAL E 561
    Length:561
    Mass (Da):65,702
    Last modified:November 1, 1995 - v1
    Checksum:i4D463062E4941859
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07642 Genomic DNA. Translation: AAA51408.1.
    PIRiJN0797.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07642 Genomic DNA. Translation: AAA51408.1 .
    PIRi JN0797.

    3D structure databases

    ProteinModelPortali P43422.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3331. M.BstVI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view ]
    Pfami PF07669. Eco57I. 1 hit.
    PF12950. TaqI_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system."
      Gonzalez E., Vasquez C.
      Gene 131:103-106(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: V.

    Entry informationi

    Entry nameiMTV1_GEOSE
    AccessioniPrimary (citable) accession number: P43422
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3