Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P43422

- MTV1_GEOSE

UniProt

P43422 - MTV1_GEOSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Modification methylase BstVI

Gene

bstVIM

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BstVI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3331. M.BstVI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BstVI (EC:2.1.1.72)
Short name:
M.BstVI
Alternative name(s):
Adenine-specific methyltransferase BstVI
Gene namesi
Name:bstVIM
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Modification methylase BstVIPRO_0000087945Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP43422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43422-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTQHSYRMH GAVYTKPIIV DLILDLTGYT SDKNLENFKL LDPSFGDGVF
60 70 80 90 100
LEAAVHRLMD SLIRRGYRPN ELIDHLGNCI RGIELRLEAY QAGRHRLQKV
110 120 130 140 150
LEGYGFSKPE INWLINQWII QADFLLWQED TTEAIKFDFV VGNPPYVRQE
160 170 180 190 200
LIQDELIKKY RKRYTTIYDR ADLYVPFIQH SLELLSEQGT LGIICSDRFT
210 220 230 240 250
KNRYGKKLRK FITDNYKVRY IVDLHKTSPF ENEVTAYPAI YVIKTKNYDK
260 270 280 290 300
SVVRAVYTEV ITSKVCQDAK DFLLSNQKPD QSSKEMKTYV FSEWFAGDEP
310 320 330 340 350
WIIQSQECRE ILRRLENRFP LIEDDVHSCK IRIGVATGAD KVYIVDPQQV
360 370 380 390 400
DIEPEVLLPL VTTADISSGR IIWSGKHVIN PFNSDGGLIN LDDFPRLKTY
410 420 430 440 450
FQQHEEIIKN RNVAKKNPSQ WFRTIDRIYP EIVHQPKLLI PDMKNTNHIV
460 470 480 490 500
KDDGAFYPHH NLYYILPGNW NIDILRAILL SSVVKFFIWS YATKMRGDTL
510 520 530 540 550
RYQAQYLRKI RLPDPKSLTN DQKERLMDER VIQSQEYLDS IVAEIYQLSK
560
TEIEIIKDAL E
Length:561
Mass (Da):65,702
Last modified:November 1, 1995 - v1
Checksum:i4D463062E4941859
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07642 Genomic DNA. Translation: AAA51408.1.
PIRiJN0797.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07642 Genomic DNA. Translation: AAA51408.1 .
PIRi JN0797.

3D structure databases

ProteinModelPortali P43422.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3331. M.BstVI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
IPR025931. TaqI_C.
[Graphical view ]
Pfami PF07669. Eco57I. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system."
    Gonzalez E., Vasquez C.
    Gene 131:103-106(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: V.

Entry informationi

Entry nameiMTV1_GEOSE
AccessioniPrimary (citable) accession number: P43422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 1, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3