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P43421

- T2V1_GEOSE

UniProt

P43421 - T2V1_GEOSE

Protein

Type-2 restriction enzyme BstVI

Gene

bstVIR

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi594. BstVI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme BstVI (EC:3.1.21.4)
    Short name:
    R.BstVI
    Alternative name(s):
    Endonuclease BstVI
    Type II restriction enzyme BstVI
    Gene namesi
    Name:bstVIR
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 224224Type-2 restriction enzyme BstVIPRO_0000077289Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR007636. Restrct_endonuc_II_XhoI.
    [Graphical view]
    PfamiPF04555. XhoI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P43421-1 [UniParc]FASTAAdd to Basket

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    MNKQASGKDT GNRGAVTGGK QMDGFADMLT NLLSKVGISN HEVFTSEKPH    50
    LPGYFRATKK WDFLVVKTND RGEKYLLAAV ELKSHVGPSF GNNLNNRVEE 100
    SLGSATDIWI AFREKAFKNS RAPWLGYLML LEDCPQSTKP VKVDEPHFEV 150
    FPEFKNASYA KRYELFCRKI VLERKYTSSC LLMSDKQGGL QGIYKEPAND 200
    LKIYPFLYSL LTHVATEAAL ISSS 224
    Length:224
    Mass (Da):25,155
    Last modified:November 1, 1995 - v1
    Checksum:iFE6767D6DBC72F1A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07643 Genomic DNA. Translation: AAA22281.1.
    PIRiJN0798.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07643 Genomic DNA. Translation: AAA22281.1 .
    PIRi JN0798.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 594. BstVI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR007636. Restrct_endonuc_II_XhoI.
    [Graphical view ]
    Pfami PF04555. XhoI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system."
      Gonzalez E., Vasquez C.
      Gene 131:103-106(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: V.

    Entry informationi

    Entry nameiT2V1_GEOSE
    AccessioniPrimary (citable) accession number: P43421
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3