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P43421

- T2V1_GEOSE

UniProt

P43421 - T2V1_GEOSE

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Protein

Type-2 restriction enzyme BstVI

Gene

bstVIR

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi594. BstVI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BstVI (EC:3.1.21.4)
Short name:
R.BstVI
Alternative name(s):
Endonuclease BstVI
Type II restriction enzyme BstVI
Gene namesi
Name:bstVIR
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Type-2 restriction enzyme BstVIPRO_0000077289Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR007636. Restrct_endonuc_II_XhoI.
[Graphical view]
PfamiPF04555. XhoI. 1 hit.
[Graphical view]
PIRSFiPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.

Sequencei

Sequence statusi: Complete.

P43421-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKQASGKDT GNRGAVTGGK QMDGFADMLT NLLSKVGISN HEVFTSEKPH
60 70 80 90 100
LPGYFRATKK WDFLVVKTND RGEKYLLAAV ELKSHVGPSF GNNLNNRVEE
110 120 130 140 150
SLGSATDIWI AFREKAFKNS RAPWLGYLML LEDCPQSTKP VKVDEPHFEV
160 170 180 190 200
FPEFKNASYA KRYELFCRKI VLERKYTSSC LLMSDKQGGL QGIYKEPAND
210 220
LKIYPFLYSL LTHVATEAAL ISSS
Length:224
Mass (Da):25,155
Last modified:November 1, 1995 - v1
Checksum:iFE6767D6DBC72F1A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07643 Genomic DNA. Translation: AAA22281.1.
PIRiJN0798.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07643 Genomic DNA. Translation: AAA22281.1 .
PIRi JN0798.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 594. BstVI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007636. Restrct_endonuc_II_XhoI.
[Graphical view ]
Pfami PF04555. XhoI. 1 hit.
[Graphical view ]
PIRSFi PIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system."
    Gonzalez E., Vasquez C.
    Gene 131:103-106(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: V.

Entry informationi

Entry nameiT2V1_GEOSE
AccessioniPrimary (citable) accession number: P43421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 1, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3