Reviewed, UniProtKB/Swiss-Prot P43421 (T2V1_BACST)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme BstVI
      Short name=R.BstVI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme BstVI
    Endonuclease BstVI

Gene names

Name:

bstVIR

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Sequence similarities	Belongs to the type II restriction enzyme XhoI family.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Type-2 restriction enzyme BstVI		PRO_0000077289

Sequences

	Sequence		Length	Mass (Da)
	P43421-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: FE6767D6DBC72F1A	FASTA	224	25,155
	10 20 30 40 50 60 MNKQASGKDT GNRGAVTGGK QMDGFADMLT NLLSKVGISN HEVFTSEKPH LPGYFRATKK 70 80 90 100 110 120 WDFLVVKTND RGEKYLLAAV ELKSHVGPSF GNNLNNRVEE SLGSATDIWI AFREKAFKNS 130 140 150 160 170 180 RAPWLGYLML LEDCPQSTKP VKVDEPHFEV FPEFKNASYA KRYELFCRKI VLERKYTSSC 190 200 210 220 LLMSDKQGGL QGIYKEPAND LKIYPFLYSL LTHVATEAAL ISSS

References

[1]	"Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system." Gonzalez E., Vasquez C. Gene 131:103-106(1993) [PubMed: 8370531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: V.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L07643 Genomic DNA. Translation: AAA22281.1.
PIR	JN0798.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	594. BstVI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 266715.
Family and domain databases
InterPro	IPR007636. Restrict_endonuc_II_XhoI. [Graphical view]
Pfam	PF04555. XhoI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2V1_BACST

Accession

Primary (citable) accession number: P43421

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)