P43421 (T2V1_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme BstVI
Short name=R.BstVI
EC=3.1.21.4

Alternative name(s):
Endonuclease BstVI
Type II restriction enzyme BstVI

Gene names

Name:

bstVIR

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Sequence similarities	Belongs to the type II restriction enzyme XhoI family.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Type-2 restriction enzyme BstVI		PRO_0000077289

Sequences

	Sequence		Length	Mass (Da)
	P43421 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: FE6767D6DBC72F1A	FASTA	224	25,155
	10 20 30 40 50 60 MNKQASGKDT GNRGAVTGGK QMDGFADMLT NLLSKVGISN HEVFTSEKPH LPGYFRATKK 70 80 90 100 110 120 WDFLVVKTND RGEKYLLAAV ELKSHVGPSF GNNLNNRVEE SLGSATDIWI AFREKAFKNS 130 140 150 160 170 180 RAPWLGYLML LEDCPQSTKP VKVDEPHFEV FPEFKNASYA KRYELFCRKI VLERKYTSSC 190 200 210 220 LLMSDKQGGL QGIYKEPAND LKIYPFLYSL LTHVATEAAL ISSS

References

[1]	"Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system." Gonzalez E., Vasquez C. Gene 131:103-106(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: V.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07643 Genomic DNA. Translation: AAA22281.1.
PIR	JN0798.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	594. BstVI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007636. Restrct_endonuc_II_XhoI. [Graphical view]
Pfam	PF04555. XhoI. 1 hit. [Graphical view]
PIRSF	PIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T2V1_GEOSE

Accession

Primary (citable) accession number: P43421

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program