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P43421 (T2V1_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BstVI

Short name=R.BstVI
EC=3.1.21.4
Alternative name(s):
Endonuclease BstVI
Type II restriction enzyme BstVI
Gene names
Name:bstVIR
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Sequence similarities

Belongs to the type II restriction enzyme XhoI family.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224Type-2 restriction enzyme BstVI
PRO_0000077289

Sequences

Sequence LengthMass (Da)Tools
P43421 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: FE6767D6DBC72F1A

FASTA22425,155
        10         20         30         40         50         60 
MNKQASGKDT GNRGAVTGGK QMDGFADMLT NLLSKVGISN HEVFTSEKPH LPGYFRATKK 

        70         80         90        100        110        120 
WDFLVVKTND RGEKYLLAAV ELKSHVGPSF GNNLNNRVEE SLGSATDIWI AFREKAFKNS 

       130        140        150        160        170        180 
RAPWLGYLML LEDCPQSTKP VKVDEPHFEV FPEFKNASYA KRYELFCRKI VLERKYTSSC 

       190        200        210        220 
LLMSDKQGGL QGIYKEPAND LKIYPFLYSL LTHVATEAAL ISSS 

« Hide

References

[1]"Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system."
Gonzalez E., Vasquez C.
Gene 131:103-106(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: V.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07643 Genomic DNA. Translation: AAA22281.1.
PIRJN0798.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE594. BstVI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007636. Restrct_endonuc_II_XhoI.
[Graphical view]
PfamPF04555. XhoI. 1 hit.
[Graphical view]
PIRSFPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2V1_GEOSE
AccessionPrimary (citable) accession number: P43421
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries