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P43420

- MTB6_BACSF

UniProt

P43420 - MTB6_BACSF

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Protein

Modification methylase Bsp6I

Gene

bsp6IM

Organism
Bacillus sp. (strain RFL6)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3315. M.Bsp6I.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase Bsp6I (EC:2.1.1.37)
Short name:
M.Bsp6I
Alternative name(s):
Cytosine-specific methyltransferase Bsp6I
Gene namesi
Name:bsp6IM
Encoded oniPlasmid pXH130 Publication
OrganismiBacillus sp. (strain RFL6)
Taxonomic identifieri72577 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Modification methylase Bsp6IPRO_0000087859Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP43420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 315314SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43420-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLQIASLFAG VGGIDLGFEQ TGYFETVWAN EYDKNAAITY QSNFKNKLII
60 70 80 90 100
DDIRNIKVED VPDFDVLLSG FPCTSFSVAG YRKGFEDEKS GDLFFETLRL
110 120 130 140 150
IVAKKPQVIF LENVKNLVGH DNGNTFKVIY EALESNGYHI KYQVLNAKDF
160 170 180 190 200
GNIPQNRERI YIVGFRNIEH YKNFNFPMPQ PLTLTIKDMI NLSDKLDDRF
210 220 230 240 250
YYTEDKCSFY SPLQEQMTSD ETIYQWRRKY VRENKSNVCP TLTANMGTGG
260 270 280 290 300
HNVPLVKTKH GIRKLTPREC FNFQGYPEDF ILPELAPTHL YKQAGNSVVV
310
PVIRRIAENI YKSML
Length:315
Mass (Da):36,315
Last modified:November 1, 1995 - v1
Checksum:iED2DD748AD33E44F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81638 Genomic DNA. Translation: CAA57293.1.
PIRiI40138.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81638 Genomic DNA. Translation: CAA57293.1 .
PIRi I40138.

3D structure databases

ProteinModelPortali P43420.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3315. M.Bsp6I.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and analysis of the plasmid-borne genes encoding the Bsp6I restriction and modification enzymes."
    Lubys A., Janulaitis A.
    Gene 157:25-29(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTB6_BACSF
AccessioniPrimary (citable) accession number: P43420
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 1, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3