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P43420

- MTB6_BACSF

UniProt

P43420 - MTB6_BACSF

Protein

Modification methylase Bsp6I

Gene

bsp6IM

Organism
Bacillus sp. (strain RFL6)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3315. M.Bsp6I.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase Bsp6I (EC:2.1.1.37)
    Short name:
    M.Bsp6I
    Alternative name(s):
    Cytosine-specific methyltransferase Bsp6I
    Gene namesi
    Name:bsp6IM
    Encoded oniPlasmid pXH130 Publication
    OrganismiBacillus sp. (strain RFL6)
    Taxonomic identifieri72577 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Modification methylase Bsp6IPRO_0000087859Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP43420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 315314SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43420-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQIASLFAG VGGIDLGFEQ TGYFETVWAN EYDKNAAITY QSNFKNKLII    50
    DDIRNIKVED VPDFDVLLSG FPCTSFSVAG YRKGFEDEKS GDLFFETLRL 100
    IVAKKPQVIF LENVKNLVGH DNGNTFKVIY EALESNGYHI KYQVLNAKDF 150
    GNIPQNRERI YIVGFRNIEH YKNFNFPMPQ PLTLTIKDMI NLSDKLDDRF 200
    YYTEDKCSFY SPLQEQMTSD ETIYQWRRKY VRENKSNVCP TLTANMGTGG 250
    HNVPLVKTKH GIRKLTPREC FNFQGYPEDF ILPELAPTHL YKQAGNSVVV 300
    PVIRRIAENI YKSML 315
    Length:315
    Mass (Da):36,315
    Last modified:November 1, 1995 - v1
    Checksum:iED2DD748AD33E44F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81638 Genomic DNA. Translation: CAA57293.1.
    PIRiI40138.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81638 Genomic DNA. Translation: CAA57293.1 .
    PIRi I40138.

    3D structure databases

    ProteinModelPortali P43420.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3315. M.Bsp6I.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of the plasmid-borne genes encoding the Bsp6I restriction and modification enzymes."
      Lubys A., Janulaitis A.
      Gene 157:25-29(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiMTB6_BACSF
    AccessioniPrimary (citable) accession number: P43420
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3