P43420 (MTB6_BACSF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase Bsp6I
Short name=M.Bsp6I
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase Bsp6I

Gene names

Name:

bsp6IM

Encoded on

Plasmid pXH13

Organism

Bacillus sp. (strain RFL6)

Taxonomic identifier

72577 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Modification methylase Bsp6I		PRO_0000087859
Sites
	Active site	73	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43420 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: ED2DD748AD33E44F	FASTA	315	36,315
	10 20 30 40 50 60 MLQIASLFAG VGGIDLGFEQ TGYFETVWAN EYDKNAAITY QSNFKNKLII DDIRNIKVED 70 80 90 100 110 120 VPDFDVLLSG FPCTSFSVAG YRKGFEDEKS GDLFFETLRL IVAKKPQVIF LENVKNLVGH 130 140 150 160 170 180 DNGNTFKVIY EALESNGYHI KYQVLNAKDF GNIPQNRERI YIVGFRNIEH YKNFNFPMPQ 190 200 210 220 230 240 PLTLTIKDMI NLSDKLDDRF YYTEDKCSFY SPLQEQMTSD ETIYQWRRKY VRENKSNVCP 250 260 270 280 290 300 TLTANMGTGG HNVPLVKTKH GIRKLTPREC FNFQGYPEDF ILPELAPTHL YKQAGNSVVV 310 PVIRRIAENI YKSML

References

[1]	"Cloning and analysis of the plasmid-borne genes encoding the Bsp6I restriction and modification enzymes." Lubys A., Janulaitis A. Gene 157:25-29(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81638 Genomic DNA. Translation: CAA57293.1.
PIR	I40138.
3D structure databases
ProteinModelPortal	P43420.
ModBase	Search...
Protein family/group databases
REBASE	3315. M.Bsp6I.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB6_BACSF

Accession

Primary (citable) accession number: P43420

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program