Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P43420 (MTB6_BACSF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase Bsp6I

Short name=M.Bsp6I
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase Bsp6I
Gene names
Name:bsp6IM
Encoded onPlasmid pXH13
OrganismBacillus sp. (strain RFL6)
Taxonomic identifier72577 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Modification methylase Bsp6I
PRO_0000087859

Regions

Domain2 – 315314SAM-dependent MTase C5-type

Sites

Active site731 By similarity

Sequences

Sequence LengthMass (Da)Tools
P43420 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: ED2DD748AD33E44F

FASTA31536,315
        10         20         30         40         50         60 
MLQIASLFAG VGGIDLGFEQ TGYFETVWAN EYDKNAAITY QSNFKNKLII DDIRNIKVED 

        70         80         90        100        110        120 
VPDFDVLLSG FPCTSFSVAG YRKGFEDEKS GDLFFETLRL IVAKKPQVIF LENVKNLVGH 

       130        140        150        160        170        180 
DNGNTFKVIY EALESNGYHI KYQVLNAKDF GNIPQNRERI YIVGFRNIEH YKNFNFPMPQ 

       190        200        210        220        230        240 
PLTLTIKDMI NLSDKLDDRF YYTEDKCSFY SPLQEQMTSD ETIYQWRRKY VRENKSNVCP 

       250        260        270        280        290        300 
TLTANMGTGG HNVPLVKTKH GIRKLTPREC FNFQGYPEDF ILPELAPTHL YKQAGNSVVV 

       310 
PVIRRIAENI YKSML 

« Hide

References

[1]"Cloning and analysis of the plasmid-borne genes encoding the Bsp6I restriction and modification enzymes."
Lubys A., Janulaitis A.
Gene 157:25-29(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X81638 Genomic DNA. Translation: CAA57293.1.
PIRI40138.

3D structure databases

ProteinModelPortalP43420.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3315. M.Bsp6I.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB6_BACSF
AccessionPrimary (citable) accession number: P43420
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries