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P43419

- T2B6_BACSF

UniProt

P43419 - T2B6_BACSF

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Protein

Type-2 restriction enzyme Bsp6I

Gene

bsp6IR

Organism
Bacillus sp. (strain RFL6)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence GCNGC and cleaves after C-2.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi485. Bsp6I.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme Bsp6I (EC:3.1.21.4)
Short name:
R.Bsp6I
Alternative name(s):
Endonuclease Bsp6I
Type II restriction enzyme Bsp6I
Gene namesi
Name:bsp6IR
Encoded oniPlasmid pXH130 Publication
OrganismiBacillus sp. (strain RFL6)
Taxonomic identifieri72577 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 174174Type-2 restriction enzyme Bsp6IPRO_0000077287Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019037. Restrct_endonuc_II_Bsp6I.
[Graphical view]
PfamiPF09504. RE_Bsp6I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43419-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALHDFVKID KSRFEDAVKA YFLWKELNAL IKNSHNRGIN FPETISETLL
60 70 80 90 100
CAAMGFELNR GTGGDAREPK TQAIIESKAT SNWDRDTTSF SPSENFDALY
110 120 130 140 150
FLRLNQRDDE LYIYNTGINS DDLKSIQVNK TQTLGDQQAQ GRRPRFSVIK
160 170
FIIEANDIEP VAKVELRTKK IIKL
Length:174
Mass (Da):19,905
Last modified:November 1, 1995 - v1
Checksum:i9A5DC728F04DCEC6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81638 Genomic DNA. Translation: CAA57292.1.
PIRiI40137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81638 Genomic DNA. Translation: CAA57292.1 .
PIRi I40137.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 485. Bsp6I.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019037. Restrct_endonuc_II_Bsp6I.
[Graphical view ]
Pfami PF09504. RE_Bsp6I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and analysis of the plasmid-borne genes encoding the Bsp6I restriction and modification enzymes."
    Lubys A., Janulaitis A.
    Gene 157:25-29(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT2B6_BACSF
AccessioniPrimary (citable) accession number: P43419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3