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P43419 (T2B6_BACSF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme Bsp6I

Short name=R.Bsp6I
EC=3.1.21.4
Alternative name(s):
Endonuclease Bsp6I
Type II restriction enzyme Bsp6I
Gene names
Name:bsp6IR
Encoded onPlasmid pXH13
OrganismBacillus sp. (strain RFL6)
Taxonomic identifier72577 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length174 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GCNGC and cleaves after C-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 174174Type-2 restriction enzyme Bsp6I
PRO_0000077287

Sequences

Sequence LengthMass (Da)Tools
P43419 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 9A5DC728F04DCEC6

FASTA17419,905
        10         20         30         40         50         60 
MALHDFVKID KSRFEDAVKA YFLWKELNAL IKNSHNRGIN FPETISETLL CAAMGFELNR 

        70         80         90        100        110        120 
GTGGDAREPK TQAIIESKAT SNWDRDTTSF SPSENFDALY FLRLNQRDDE LYIYNTGINS 

       130        140        150        160        170 
DDLKSIQVNK TQTLGDQQAQ GRRPRFSVIK FIIEANDIEP VAKVELRTKK IIKL 

« Hide

References

[1]"Cloning and analysis of the plasmid-borne genes encoding the Bsp6I restriction and modification enzymes."
Lubys A., Janulaitis A.
Gene 157:25-29(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X81638 Genomic DNA. Translation: CAA57292.1.
PIRI40137.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE485. Bsp6I.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019037. Restrct_endonuc_II_Bsp6I.
[Graphical view]
PfamPF09504. RE_Bsp6I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2B6_BACSF
AccessionPrimary (citable) accession number: P43419
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries